Allele Dmel\cmet^dsRNA.cGa

General Information
Symbol	Dmel\cmet^dsRNA.cGa	Species	D. melanogaster
Name		FlyBase ID	FBal0180463
Feature type	allele	Associated gene	Dmel\cmet

Allele class
Mutagen	in vitro construct - RNAi
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - RNAi (Goshima and Vale, 2003)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Template corresponding to cmet sequences. (Goshima and Vale, 2003)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In
	astral microtubule (Goshima and Vale, 2003) mitosis & nuclear chromosome (Goshima and Vale, 2003) spindle microtubule (Goshima and Vale, 2003) spindle pole (Goshima and Vale, 2003)
Detailed Description
	Statement Reference Treatment of S2 cells with cmet^dsRNA.cGa results in an increased frequency of pro-metaphase-like cells with bipolar spindles and misaligned chromosomes that are detached from the metaphase plate during mitosis. The mitotic index is slightly higher than in wild-type. cmet^dsRNA.cGa treated S2 cells exhibit discernible changes in the morphology or microtubule pattern in interphase cells. (Goshima and Vale, 2003)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference Treatment of S2 cells with cmet^dsRNA.cGa and Klp3A^dsRNA.cGa results in a more severe chromosome misalignment than in single RNAi treatment. The percentage cells in prometaphase, with at least one misaligned chromosome per bipolar spindle is almost doubled in the double RNAi mutant compared to each singular mutant. Treatment of S2 cells with cmet^dsRNA.cGa and nod^dsRNA.cGa results in a more severe chromosome misalignment than in single RNAi treatment, with ~50% of prometaphase cells exhibiting at least one misaligned chromosome in the bipolar spindle. Treatment of S2 cells with cmet^dsRNA.cGa, Klp3A^dsRNA.cGa and nod^dsRNA.cGa greatly reduces the numbers of cells with normal metaphase chromosomal alignments (3% of mitotic cells). The number of prometaphase cells with at least one misaligned chromosome observed in the bipolar spindle is increased in the triple mutant to ~72%, higher than the single or double mutants. (Goshima and Vale, 2003)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

Comments
	dsRNA has been synthesised from the template and transfected into S2 cells to assay the role of cmet in mitosis. (Goshima and Vale, 2003)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	cmet^dsRNA.cGa
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Goshima and Vale, 2003, J. Cell Biol. 162(6): 1003--1016 The roles of microtubule-based motor proteins in mitosis: comprehensive RNAi analysis in the Drosophila S2 cell line. [FBrf0162187]

Allele Dmel\cmetdsRNA.cGa

Allele Dmel\cmet^dsRNA.cGa