|Feature type||allele||Associated gene||Dmel\cmet|
|Mutagen||in vitro construct - RNAi|
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|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
|Phenotype Manifest In|
mitosis & nuclear chromosome
Treatment of S2 cells with cmetdsRNA.cGa results in an increased frequency of pro-metaphase-like cells with bipolar spindles and misaligned chromosomes that are detached from the metaphase plate during mitosis. The mitotic index is slightly higher than in wild-type. cmetdsRNA.cGa treated S2 cells exhibit discernible changes in the morphology or microtubule pattern in interphase cells.
|Phenotype Manifest In|
Treatment of S2 cells with cmetdsRNA.cGa and Klp3AdsRNA.cGa results in a more severe chromosome misalignment than in single RNAi treatment. The percentage cells in prometaphase, with at least one misaligned chromosome per bipolar spindle is almost doubled in the double RNAi mutant compared to each singular mutant. Treatment of S2 cells with cmetdsRNA.cGa and noddsRNA.cGa results in a more severe chromosome misalignment than in single RNAi treatment, with ~50% of prometaphase cells exhibiting at least one misaligned chromosome in the bipolar spindle. Treatment of S2 cells with cmetdsRNA.cGa, Klp3AdsRNA.cGa and noddsRNA.cGa greatly reduces the numbers of cells with normal metaphase chromosomal alignments (3% of mitotic cells). The number of prometaphase cells with at least one misaligned chromosome observed in the bipolar spindle is increased in the triple mutant to ~72%, higher than the single or double mutants.
|Complementation & Rescue Data|
|Stocks ( 0 )|
|Notes on Origin|
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 1 )|
|Secondary FlyBase IDs|
|References ( 1 )|