Allele Dmel\baz^{dsRNA.Sym.Scer\UAS}

General Information
Symbol	Dmel\baz^{dsRNA.Sym.Scer\UAS}	Species	D. melanogaster
Name		FlyBase ID	FBal0180866
Feature type	allele	Associated gene	Dmel\baz

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - RNAi
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Pinheiro and Montell, 2004) in vitro construct - RNAi (Pinheiro and Montell, 2004)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Sense-antisense transcription (driven by two convergent arrays of Scer\UAS promoter and regulatory sequences) of a 2 kb fragment of baz (nucleotides 28506 to 30554 of genomic clone - GenBank:AE003504) results in double stranded RNA (dsRNA) expression. (Pinheiro and Montell, 2004)
Carried in construct	P{Sym-baz} (Pinheiro and Montell, 2004)
Cytology
Phenotypic Data
Phenotypic Class
	lethal, with Scer\GAL4^Act5C.PI (Pinheiro and Montell, 2004) lethal, with Scer\GAL4^αTub84B.PL (Pinheiro and Montell, 2004)
Phenotype Manifest In
	border follicle cell, with Scer\GAL4^slbo.2.6 (Pinheiro and Montell, 2004) border follicle cell, with Scer\GAL4^slbo.2.6, baz⁴ (Pinheiro and Montell, 2004) border follicle cell, with Scer\GAL4^slbo.2.6, Scer\GAL4^upd (Pinheiro and Montell, 2004)
Detailed Description
	Statement Reference Border cell migration is incomplete in 14% of stage 10 egg chambers from (baz^Scer\UAS.Sym baz^{Scer\UAS.a.Sym})X2; Scer\GAL4^slbo.2.6 flies. This increases to 33% in flies also heterozygous for baz⁴ and is also increased by the presence of Scer\GAL4^upd in addition to Scer\GAL4^slbo.2.6. The organisation of border cell clusters is abnormal in these flies. (Pinheiro and Montell, 2004) Border cell migration is incomplete in 14% of stage 10 egg chambers from (baz[dsRNA.Sym.Scer\UAS])X2; Scer\GAL4[slbo.2.6] flies. This increases to 33% in flies also heterozygous for baz[4] and is also increased by the presence of Scer\GAL4[upd] in addition to Scer\GAL4[slbo.2.6]. The organisation of border cell clusters is abnormal in these flies. (Pinheiro and Montell, 2004)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

Comments
	P{Sym-baz} contains baz sequences flanked on both sides by Scer\UAS regulatory sequences in opposite orientation (producing alleles baz^Scer\UAS.Sym and baz^{Scer\UAS.a.Sym}). Expression from both of these Scer\UAS sequences should result in both sense and antisense copies of the baz sequences, producing dsRNA which can cause RNAi (dsRNA interference). (Pinheiro and Montell, 2004)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	baz^{dsRNA.Sym.Scer\UAS} baz^Scer\UAS.Sym
Name Synonym
Secondary FlyBase IDs
	FBal0180865
References ( 1 )
Research paper	Pinheiro and Montell, 2004, Development 131(21): 5243--5251 Requirement for Par-6 and Bazooka in Drosophila border cell migration. [FBrf0180163]

Allele Dmel\bazdsRNA.Sym.Scer\UAS

Allele Dmel\baz^{dsRNA.Sym.Scer\UAS}