Allele Dmel\Tap42^Δ305b

General Information
Symbol	Dmel\Tap42^Δ305b	Species	D. melanogaster
Name		FlyBase ID	FBal0181305
Feature type	allele	Associated gene	Dmel\Tap42

Allele class
Mutagen	P-element activity
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	P-element activity (Cygnar et al., 2005)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	P{lacW}Nnp-1^k07826 (Cygnar et al., 2005)
Nature of the lesion	Statement Reference Imprecise excision of the P{lacW}Nnp-1^k07826 element, which removes the element and 1.6kb of sequences downstream. This deletion extends through the transcription and translation start sites of Tap42 and removes the first 8 codons of the Tap42 coding region. (Cygnar et al., 2005)
Caused by aberration	Df(2L)Tap42^Δ305b (Cygnar et al., 2005)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| recessive \| third instar larval stage (Cygnar et al., 2005)
Phenotype Manifest In
	embryonic/larval brain (Cygnar et al., 2005) mitosis (Cygnar et al., 2005) spindle (Cygnar et al., 2005)
Detailed Description
	Statement Reference Tap42^Δ305b animals (Df(2L)Tap42^Δ305b homozygotes in which lack of Nnp-1 and CG6523 function has been rescued using the P{Nnp-1^t5.1} transgene) progress to the early third larval instar with a slight developmental delay and die shortly thereafter, between 96 and 120 hours after egg laying. Tap42^Δ305b larvae (Df(2L)Tap42^Δ305b homozygotes in which lack of Nnp-1 and CG6523 function has been rescued using the P{Nnp-1^t5.1} transgene) have smaller and poorly organised mitotic spindles in the central nervous system compared to controls and most mitotic chromosomes are in a highly condensed metaphase configuration (no mitotic cells in anaphase or telophase have been observed). Third larval instar mutant brains contain pyknotic nuclei. Clones of cells lacking Tap42 function (homozygous for Df(2L)Tap42^Δ305b, and with lack of Nnp-1 and CG6523 function rescued by the P{Nnp-1^t5.1} transgene) show no change in cell size compared to control cells, and the cell cycle phasing of the mutant cells is indistinguishable from that of wild-type controls. Although clones of cells lacking Tap42 function (homozygous for Df(2L)Tap42^Δ305b, and with lack of Nnp-1 and CG6523 function rescued by the P{Nnp-1^t5.1} transgene) can be readily identified in wing discs 48 hours after induction, by 72 hours after induction they are no longer present. Mutant clones induced using the Minute technique can persist to 72 hours, but show signs of massive cell death, with highly condensed pyknotic nuclei, and these clones are eliminated at later timepoints. Mutant clones in the eye imaginal discs can survive beyond the 72 hour period in the mitotically quiescent region posterior to the morphogenetic furrow, but not in the mitotically active anterior region. (Cygnar et al., 2005)
External Data
Linkouts
Interactions
Phenotypic Class
NOT Suppressor of
Statement Reference Tap42^Δ305b is a non-suppressor of increased cell size phenotype of Pten^dj189 (Cygnar et al., 2005)
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference Clones of Pten^dj189 Tap42^Δ305b double mutant cells (homozygous for Pten^dj189 and Df(2L)Tap42^Δ305b and with the lack of Nnp-1 and CG6523 function caused by Df(2L)Tap42^Δ305b rescued using the P{Nnp-1^t5.1} transgene) show the characteristic increase in cell size and S/G₂ content of Pten^dj189 single mutants. (Cygnar et al., 2005)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	Tap42^Δ305b is rescued by Scer\GAL4^tub.PU/Tap42^Scer\UAS.cCa (Cygnar et al., 2005)
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Tap42^Δ305b
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Cygnar et al., 2005, Genetics 170(2): 733--740 The phosphatase subunit tap42 functions independently of target of rapamycin to regulate cell division and survival in Drosophila. [FBrf0188576]

Allele Dmel\Tap42Δ305b

Allele Dmel\Tap42^Δ305b