Allele Dmel\Sh^mns

General Information
Symbol	Dmel\Sh^mns	Species	D. melanogaster
Name	minisleep	FlyBase ID	FBal0181553
Feature type	allele	Associated gene	Dmel\Sh
Also Known As	mns

Map ( GBrowse )
Allele class
Mutagen	ethyl methanesulfonate
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	ethyl methanesulfonate (Circelli et al., 2005)
Mutations Mapped to the Genome

Type Location Additional Notes References point mutation X:17,845,473..17,845,473 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. Position of mutation on reference sequence inferred by FlyBase curator based on author statement (see Figure 3 of FBrf0184967) evidence=experimental na_change=C17845473T pr_change=T203I\|Sh-PD,T236I\|Sh-PC,T248I\|Sh-PE,T248I\|Sh-P B,T248I\|Sh-PG,T248I\|Sh-PF,T203I\|Sh-PA reported_na_change=C?T reported_pr_change=T?I (Circelli et al., 2005)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Amino acid replacement: T?I. Nucleotide substitution: C?T. The mutation is within exon 9. (Circelli et al., 2005)
Cytology
Phenotypic Data
Phenotypic Class
	behavior defective (Yuan et al., 2006, Circelli et al., 2005, Weber et al., 2009) fertile (Circelli et al., 2005) short lived (Circelli et al., 2005) viable (Circelli et al., 2005)
Phenotype Manifest In

Detailed Description
	Statement Reference Mutant flies have normal mushroom body morphology. (Pfeiffenberger and Allada, 2012) The average amount of daily sleep in Sh[mns] flies is reduced compared to wild type (584 +/- 13 minutes for the mutant flies versus 965 +/- 15 minutes for wild type). The EC[[50]] value for the volatile anesthetics isoflurane and sevoflurane are significantly increased in the mutant flies compared to wild type. (Weber et al., 2009) Sh^mns flies respond to 5-HTP treatment with an increase in daily sleep and a significant reduction in night-time sleep bout length, compared to controls where daily sleep is increased but there is no change in night-time sleep bout length. (Yuan et al., 2006) Mutant flies exhibit a decrease in the daily sleep amount. This is mainly due to a decrease in the duration of sleep episodes rather than in their number. Mutant flies maintain a rhythmic modulation of locomotor activity with a period of about 24h. the penetrance of this phenotype is not complete. When sleep deprived, mutants (like wild-type) show an increase in sleep duration and intensity. However the increase in intensity is not as marked as seen in wild-type. Mutant flies show no obvious developmental or other behavioural phenotype. However the performance of mutant flies in complex stimulus or thermal response tests is considerably less reduced after sleep deprivation compared to wild-type. Mutant animals exhibit a transient shaking of the legs and scissoring of the wings when recovering from diethyl ether anaesthesia. This phenotype is dominant and persists in amputated legs. It is not seen when intact flies are exposed to chloroform, haloethane, isoflurane or enflurane. Mutants have a shorter lifespan than controls. (Circelli et al., 2005)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 1 )
Bloomington	24149 Sh^mns
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 4 )
Reported As
Symbol Synonym	E1174 mns (Circelli et al., 2005, Yuan et al., 2006) Sh^mns (Weber et al., 2009, Pfeiffenberger and Allada, 2012)
Name Synonym	minisleep (Circelli et al., 2005, Yuan et al., 2006, Weber et al., 2009, )
Secondary FlyBase IDs

References ( 4 )
Research paper	Pfeiffenberger and Allada, 2012, PLoS Genet. 8(10): e1003003 Cul3 and the BTB Adaptor Insomniac Are Key Regulators of Sleep Homeostasis and a Dopamine Arousal Pathway in Drosophila. [FBrf0219613] Weber et al., 2009, Anesthesiology 110(2): 313--316 Increased volatile anesthetic requirement in short-sleeping Drosophila mutants. [FBrf0206678] Yuan et al., 2006, Curr. Biol. 16(11): 1051--1062 A sleep-promoting role for the Drosophila serotonin receptor 1A. [FBrf0195242]
Review	Circelli et al., 2005, Nature 434: 1087--1092 Reduced sleep in Drosophila shaker mutants. [FBrf0184967]

Allele Dmel\Shmns

Allele Dmel\Sh^mns