FlyBase Allele Report: Dmel\Roc1b[dc3]

General Information

Symbol

Dmel\Roc1b^dc3

Species

D. melanogaster

Name

FlyBase ID

FBal0181840

Feature type

allele

Associated gene

Dmel\Roc1b

Associated Insertion(s)

Carried in Construct

Allele class

loss of function allele

Mutagen

Nature of the Allele

Allele class

loss of function allele

(Donaldson et al., 2004)

Mutagen

I-SceI

(Donaldson et al., 2004)

I-CreI

(Donaldson et al., 2004)

recombination

(Donaldson et al., 2004)

FLPase

(Donaldson et al., 2004)

ends-in gene targeting

(Donaldson et al., 2004)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Cytology

Nature of the lesion

Statement

Reference

Deletion of the Roc1b coding region

(Donaldson et al., 2004)

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Phenotypic Data

Phenotypic Class

male sterile

(Roberts et al., 2012, Donaldson et al., 2004)

male sterile (with Roc1b^F28M)

(Donaldson et al., 2004)

viable

(Roberts et al., 2012, Donaldson et al., 2004)

Phenotype Manifest In

spermatid

(Arama et al., 2007)

spermatozoon

(Donaldson et al., 2004)

Detailed Description

Statement

Reference

Roc1b^dc3 mutant clones in sensory neurons in adult wing do not display any defects in injury-induced axon degeneration (following an axotomy, the severed axons are cleared away normally).

(Neukomm et al., 2017)

Homozygous spermatids show severe defects in individualisation.

(Arama et al., 2007)

Sperm cells in the testes of Roc1b^dc3 males are completely immotile, but have completed the individualization process correctly.

(Donaldson et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Roc1b^dc3 has male sterile phenotype, suppressible by Roc1a::Roc1b^BNAR.Tag:V5

(Reynolds et al., 2008)

Roc1b^dc3 has male sterile phenotype, suppressible by Roc1a^grf.Roc1b

(Donaldson et al., 2004)

NOT suppressed by

Statement

Reference

Roc1b^dc3 has male sterile phenotype, non-suppressible by Roc2^grf.Roc1b

(Donaldson et al., 2004)

NOT Suppressor of

Statement

Reference

Roc1b^dc3 is a non-suppressor of abnormal neuroanatomy | somatic clone | adult stage phenotype of Sarm^{ΔARM.UAS.Tag:MYC}, Scer\GAL4^VGlut-OK371

(Neukomm et al., 2017)

Phenotype Manifest In

NOT Suppressor of

Statement

Reference

Roc1b^dc3 is a non-suppressor of sensory neuron | somatic clone | adult stage phenotype of Sarm^{ΔARM.UAS.Tag:MYC}, Scer\GAL4^VGlut-OK371

(Neukomm et al., 2017)

Roc1b^dc3 is a non-suppressor of axon | somatic clone | adult stage phenotype of Sarm^{ΔARM.UAS.Tag:MYC}, Scer\GAL4^VGlut-OK371

(Neukomm et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The axon degeneration in sensory neurons clones in the adult wing expressing Ect4^{ΔARM.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^VGlut-OK371 can be suppressed by combination with Roc1b^dc3.

(Neukomm et al., 2017)

Roc1a::Roc1b^{BNAR.T:SV5\V5} can rescue the male sterility caused by Roc1b^dc3.

(Reynolds et al., 2008)

The male sterility of Roc1b^dc3 flies is partially rescued by expression of the transgene Roc1a^grf.Roc1b.

(Donaldson et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Roc1b^dc3 is rescued by Roc1b^grf.Tag:V5

(Reynolds et al., 2008)

Roc1b^dc3 is rescued by Roc1b^{BNAR.T:Roc1a,T:SV5\V5}

(Reynolds et al., 2008)

Partially rescued by

Roc1b^dc3 is partially rescued by Roc1b^grf.Tag:FLAG

(Donaldson et al., 2004)

Roc1b^dc3 is partially rescued by Roc1b^+t1.17

(Donaldson et al., 2004)

Not rescued by

Roc1b^dc3 is not rescued by Roc1b^Roc1a.Tag:V5

(Reynolds et al., 2008)

Comments

Roc1b^{Roc1a.T:SV5\V5} fails to rescue the male sterility caused by Roc1b^dc3.

Roc1b^grf.T:SV5\V5 can rescue the male sterility caused by Roc1b^dc3.

(Reynolds et al., 2008)

Expression of either Roc1b^{grf.T:Zzzz\FLAG} or Roc1b^+t1.17 rescues the fertility of Roc1b^dc3 males to ~70% of wild-type levels.

(Donaldson et al., 2004)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

34051

w^*; Roc1b^dc3/TM3, P{ActGFP}JMR2, Ser¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Roc1b^dc3

(Neukomm et al., 2017, Roberts et al., 2012, Reynolds et al., 2008)

roc1b^dc3

(Arama et al., 2007)

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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