The expression of RhebScer\UAS.cPa under the control of Scer\GAL4ninaE.PU does not suppress the expansion in endosome-like vesicles and multivesicular bodies observed in the photoreceptors of PIP4K29 homozygous late pupae reared in light conditions.
The ability of Scer\GAL4insc-Mz1407-driven expression of RhebScer\UAS.cPa to induce central brain neuroblast reactivation in the absence of dietary amino acids is prevented by co-expression of ChroKK105370.
Heterozygosity for TorΔ6B in a RhebScer\UAS.cPa, Scer\GAL4elav.PLu background rescues the phototaxis phenotype and photoreceptor axon guidance abnormalities to almost control levels, and causes a significant rescue of the synapse overgrowth phenotype.
Co-expression of SNF1ATD.Scer\UAS significantly rescues the phototaxis and axon guidance phenotypes, while it enhances the synapse overgrowth and excitatory junctional potential phenotypes, of RhebScer\UAS.cPa, Scer\GAL4elav.PLu flies.
Expression of RhebScer\UAS.cPa in Hr51LL04325 MARCM neuroblast clones partially suppresses the developmental regrowth defect. In addition these double mutants exhibit severe axon guidance defects. In these clones, γ axons do not occupy the entire adult lobe but instead grow to the dorsal and ventral extremities of the adult γ-lobe and in some cases only to its ventral portion.
Overexpression of RhebScer\UAS.cPa in the developing eye enhances the phenotype seen in mutant flies co-expressing Hsap\HTT152Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP and Hsap\HTT48Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1 under the control of Scer\GAL4GMR.PF, with a severe rough eye phenotype and enhancement of pigment cell loss.
Overexpression of RhebScer\UAS.cPa in the developing eye of Hsap\HTT18Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1 mutants (both transgene under the control of Scer\GAL4GMR.PF results in a rough eye phenotype with bristle disorganization.
Co-expression of RhebScer\UAS.cPa in a Hsap\HTT98Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1 mutant brain (with both transgenes under the control of Scer\GAL4elav-C155) does not significantly increase the average number of brain aggregate inclusions compared to flies expressing Hsap\HTT98Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1 alone, but does increase the average aggregate size.