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General Information
Symbol
Dmel\RhebUAS.cPa
Species
D. melanogaster
Name
UAS construct a of Patel
FlyBase ID
FBal0181935
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-Rheb, UAS-Rheb.Pa
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt sequences regulate expression of a XhoI/BglII fragment of a full length Rheb EST (GH15143).

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Third instar larvae expressing RhebUAS.cPa under the control of Scer\GAL4AB1 do not show significant changes in the size of salivary gland cells, as compared to controls.

Expression of RhebScer\UAS.cPa under the control of Scer\GAL4insc-Mz1407 is capable of driving central brain neuroblast reactivation in the absence of dietary amino acids.

The expression of RhebScer\UAS.cPa under the control of Scer\GAL4en-e16E leads to a significant increase in the wing posterior compartment area, as compared to controls.

Expression of RhebScer\UAS.cPa under the control of Scer\GAL4phm.PO leads to decreased number of autophagic vesicles (marked with Atg8a protein).

The expression of RhebScer\UAS.cPa under the control of Scer\GAL4E(spl)m6-BFM.PF leads to significantly increased numbers of abdominal lateral and dorsal, but not ventral, adult muscle precursor cells in third instar larvae, as compared to controls; this expression does not affect the number of mitotic adult muscle precursor cells at embryonic stages, as compared to controls.

Expression via Scer\GAL4wor.PA and Scer\GAL80ase.PN does not prevent the normal shrinkage and disappearance of pupal neuroblasts.

Expression of RhebScer\UAS.cPa under the control of Scer\GAL4da.G32 results in an increase in larval body weight compared to controls.

Flies expressing RhebScer\UAS.cPa in the midgut, under the control of Scer\GAL4esg-NP5130 and Scer\GAL80ts.αTub84B at the restrictive temperature of 29[o]C for two weeks, results in considerably fewer epithelial midgut cells, accounting for 10.4% of the total population. Some of the remaining intestinal stem cells are larger.

Overexpression of RhebScer\UAS.cPa under the control of Scer\GAL4GMR.PF increases S6k phosphorylation.

Expression of RhebScer\UAS.cPa under the control of Scer\GAL4Mef2.PR is able to completely suppress the formation of autophagosomes in the muscles of chloroquine-treated larvae starved on low-nutrient food for 6 hours.

Scer\GAL4elav.PLu-mediated expression of RhebScer\UAS.cPa produces phototaxis deficits and a significantly lower phototaxis index. It also results in a failure of some R7 and R8 photoreceptors to terminate at their proper locations, produces substantial synaptic overgrowth at the larval neuromuscular junction, and increased excitatory junctional potentials.

The Scer\GAL4elav.PLu, RhebScer\UAS.cPa phototaxis deficits are significantly rescued when flies are reared on a yeast-restricted or calorie-restricted diet.

Raised at 25[o]C, the Scer\GAL4elav.PLu, RhebScer\UAS.cPa axon guidance phenotype is significantly rescued when flies are reared on a yeast-restricted or calorie-restricted or sugar-restricted diet.

Raised at 21[o]C, the Scer\GAL4elav-C155, RhebScer\UAS.cPa flies show a mild axon guidance phenotype that is significantly rescued when flies are reared on a yeast-restricted or calorie-restricted diet.

The Scer\GAL4elav.PLu, RhebScer\UAS.cPa synaptic overgrowth and increased excitatory junctional potential phenotypes cannot be rescued by dietary restriction. Flies raised on a sugar restricted diet actually show an increase in synaptic overgrowth.

Expression of RhebScer\UAS.cPa in mushroom body γ-neurons, under the control of Scer\GAL4Tab2-201Y does not affect mushroom body morphology.

Overexpression of RhebScer\UAS.cPa using either Scer\GAL4elav-C155 or Scer\GAL4P2.4.Pdf increases ethanol sensitivity.

Expression of RhebScer\UAS.cPa under the control of Scer\GAL4sca.PU in the adult thorax leads to an increase in the frequency of bristle twinning, and delay or premature arrest of the cell cycle, as compared to controls.

Overexpression of RhebScer\UAS.cPa via Scer\GAL4GMR.PF causes a hyperplastic phenotype in the eye.

Expression of RhebScer\UAS.cPa in the developing eye under the control of Scer\GAL4GMR.PF results in a rough eye phenotype with bristle disorganization.

Expression of RhebScer\UAS.cPa driven by Scer\GAL4ap-md544 in the dorsal epithelial of the wing disc results in enlargement of the dorsal compartment, which causes the wing to curve downwards. When expression of RhebScer\UAS.cPa is driven by Scer\GAL4ap-md544 in the posterior compartment of the wing, the posterior area is increased but cell density is lower and total cell number is not significantly different to wild type. Therefore, cell size is increased. The anterior compartment of these wings is reduced compared to wild type, while the cell density is increased.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Suppressed by
NOT suppressed by
Enhancer of
Suppressor of
NOT Suppressor of
Other
Phenotype Manifest In
Enhanced by
NOT Enhanced by
Suppressed by
Statement
Reference
NOT suppressed by
Enhancer of
Suppressor of
Statement
Reference
NOT Suppressor of
Other
Additional Comments
Genetic Interactions
Statement
Reference

PIP4K29 third instar larvae expressing RhebUAS.cPa under the control of Scer\GAL4AB1 show significantly bigger salivary gland cells, as compared to PIP4K29 single mutants.

The expression of RhebScer\UAS.cPa under the control of Scer\GAL4ninaE.PU does not suppress the expansion in endosome-like vesicles and multivesicular bodies observed in the photoreceptors of PIP4K29 homozygous late pupae reared in light conditions.

The ability of Scer\GAL4insc-Mz1407-driven expression of RhebScer\UAS.cPa to induce central brain neuroblast reactivation in the absence of dietary amino acids is prevented by co-expression of ChroKK105370.

The increased wing posterior compartment area induced by the expression of RhebScer\UAS.cPa under the control of Scer\GAL4en-e16E is suppressed by the co-expression of nclbNIG.6751R.

The co-expression of RhebScer\UAS.cPa does not suppress the increased midgut cell size resulting from the expression of Atg9HMS01246 under the control of Scer\GAL4Myo31DF-NP0001.

Scer\GAL4ppk.PG-mediated co-expression of pathA.Scer\UAS.T:Avic\GFP and RhebScer\UAS.cPa promote dendritic growth, whereas expression of either alone has no such effect.

Expression of RhebScer\UAS.cPa does not suppress the enlarged bouton phenotype seen when Vap-33AP58S.cRa.Scer\UAS is expressed under the control of Scer\GAL4elav-C155.

Expression of RhebScer\UAS.cPa under the control of Scer\GAL4ppk.PG enhances the dendrite pruning defects seen in Mical15256 mutant ddaC neuron clones at 16 hours after puparium formation.

Heterozygosity for TorΔ6B in a RhebScer\UAS.cPa, Scer\GAL4elav.PLu background rescues the phototaxis phenotype and photoreceptor axon guidance abnormalities to almost control levels, and causes a significant rescue of the synapse overgrowth phenotype.

Co-expression of SNF1ATD.Scer\UAS significantly rescues the phototaxis and axon guidance phenotypes, while it enhances the synapse overgrowth and excitatory junctional potential phenotypes, of RhebScer\UAS.cPa, Scer\GAL4elav.PLu flies.

Co-expression of raptorGD5138 or S6kGD6646 significantly rescues the axon guidance phenotypes of RhebScer\UAS.cPa, Scer\GAL4elav.PLu flies.

The axon guidance phenotypes of RhebScer\UAS.cPa, Scer\GAL4elav.PLu flies are not significantly rescued in a rictorΔ1/rictorΔ2 or Sin1e03756/Sin1e03756 background.

Co-expression of raptorGD5138 or S6kGD6646 does not rescue the RhebScer\UAS.cPa, Scer\GAL4elav.PLu synapse overgrowth phenotype.

The RhebScer\UAS.cPa, Scer\GAL4elav.PLu synapse overgrowth phenotype is significantly rescued in a rictorΔ1/rictorΔ2 or Sin1e03756/Sin1e03756 background.

Expression of RhebScer\UAS.cPa in Hr51LL04325 MARCM neuroblast clones partially suppresses the developmental regrowth defect. In addition these double mutants exhibit severe axon guidance defects. In these clones, γ axons do not occupy the entire adult lobe but instead grow to the dorsal and ventral extremities of the adult γ-lobe and in some cases only to its ventral portion.

Co-expression of Rab11Scer\UAS.P\T.T:Avic\GFP-YFP or nufEP3324 does not enhance the bristle twinning phenotype of flies expressing RhebScer\UAS.cPa under the control of Scer\GAL4sca.PU.

Co-expression of RhebScer\UAS.cPa enhances the bristle twinning phenotype seen in flies expressing l(2)gl3A.Scer\UAS under the control of Scer\GAL4sca.PU.

Co-expression of Sesnd04539 with RhebScer\UAS.cPa via Scer\GAL4GMR.PF suppresses the RhebScer\UAS.cPa hyperplastic eye phenotype.

Xenogenetic Interactions
Statement
Reference

Overexpression of RhebScer\UAS.cPa in the developing eye enhances the phenotype seen in mutant flies co-expressing Hsap\HTT152Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP and Hsap\HTT48Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1 under the control of Scer\GAL4GMR.PF, with a severe rough eye phenotype and enhancement of pigment cell loss.

Overexpression of RhebScer\UAS.cPa in the developing eye of Hsap\HTT18Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1 mutants (both transgene under the control of Scer\GAL4GMR.PF results in a rough eye phenotype with bristle disorganization.

Co-expression of RhebScer\UAS.cPa in a Hsap\HTT98Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1 mutant brain (with both transgenes under the control of Scer\GAL4elav-C155) does not significantly increase the average number of brain aggregate inclusions compared to flies expressing Hsap\HTT98Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1 alone, but does increase the average aggregate size.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
RhebScer\UAS.cPa
RhebUAS.cPa
Name Synonyms
UAS construct a of Patel
Secondary FlyBase IDs
    References (30)