Allele Dmel\Rheb^Scer\UAS.cPa

General Information
Symbol	Dmel\Rheb^Scer\UAS.cPa	Species	D. melanogaster
Name	UAS construct a of Patel	FlyBase ID	FBal0181935
Feature type	allele	Associated gene	Dmel\Rheb

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Patel et al., 2003)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS sequences regulate expression of a XhoI/BglII fragment of a full length Rheb EST (GH15143). (Patel et al., 2003)
Carried in construct	P{UAS-Rheb.Pa} (Patel et al., 2003, Dimitroff et al., 2012, Lee et al., 2007, Lee et al., 2010, Doumanis et al., 2009, Eddison et al., 2011, Yaniv et al., 2012)
Cytology
Phenotypic Data
Phenotypic Class
	cell size defective, with Scer\GAL4^ap-md544 (Patel et al., 2003) chemical sensitive, with Scer\GAL4^P2.4.Pdf (Eddison et al., 2011) neuroanatomy defective \| nutrition conditional, with Scer\GAL4^elav.PLu (Dimitroff et al., 2012) neuroanatomy defective \| nutrition conditional, with Scer\GAL4^elav-C155 (Dimitroff et al., 2012) neurophysiology defective, with Scer\GAL4^elav.PLu (Dimitroff et al., 2012) phototaxis defective \| nutrition conditional, with Scer\GAL4^elav.PLu (Dimitroff et al., 2012) visible, with Scer\GAL4^GMR.PF (Lee et al., 2010)
Phenotype Manifest In
	embryonic/larval neuromuscular junction, with Scer\GAL4^elav.PLu (Dimitroff et al., 2012) eye, with Scer\GAL4^GMR.PF (Lee et al., 2010) photoreceptor cell R7 \| nutrition conditional, with Scer\GAL4^elav.PLu (Dimitroff et al., 2012) photoreceptor cell R7 \| nutrition conditional, with Scer\GAL4^elav-C155 (Dimitroff et al., 2012) photoreceptor cell R8 \| nutrition conditional, with Scer\GAL4^elav.PLu (Dimitroff et al., 2012) photoreceptor cell R8 \| nutrition conditional, with Scer\GAL4^elav-C155 (Dimitroff et al., 2012) wing \| anterior compartment, with Scer\GAL4^ap-md544 (Patel et al., 2003) wing \| dorsal compartment, with Scer\GAL4^ap-md544 (Patel et al., 2003) wing \| posterior compartment, with Scer\GAL4^ap-md544 (Patel et al., 2003)
Detailed Description
	Statement Reference Scer\GAL4[elav.PLu]-mediated expression of Rheb[Scer\UAS.cPa] produces phototaxis deficits and a significantly lower phototaxis index. It also results in a failure of some R7 and R8 photoreceptors to terminate at their proper locations, produces substantial synaptic overgrowth at the larval neuromuscular junction, and increased excitatory junctional potentials. The Scer\GAL4[elav.PLu], Rheb[Scer\UAS.cPa] phototaxis deficits are significantly rescued when flies are reared on a yeast-restricted or calorie-restricted diet. Raised at 25[o]C, the Scer\GAL4[elav.PLu], Rheb[Scer\UAS.cPa] axon guidance phenotype is significantly rescued when flies are reared on a yeast-restricted or calorie-restricted or sugar-restricted diet. Raised at 21[o]C, the Scer\GAL4[elav-C155], Rheb[Scer\UAS.cPa] flies show a mild axon guidance phenotype that is significantly rescued when flies are reared on a yeast-restricted or calorie-restricted diet. The Scer\GAL4[elav.PLu], Rheb[Scer\UAS.cPa] synaptic overgrowth and increased excitatory junctional potential phenotypes cannot be rescued by dietary restriction. Flies raised on a sugar restricted diet actually show an increase in synaptic overgrowth. (Dimitroff et al., 2012) Expression of Rheb[Scer\UAS.cPa] in mushroom body γ-neurons, under the control of Scer\GAL4[Tab2-201Y] does not affect mushroom body morphology. (Yaniv et al., 2012) Overexpression of Rheb[Scer\UAS.cPa] using either Scer\GAL4[elav-C155] or Scer\GAL4[P2.4.Pdf] increases ethanol sensitivity. (Eddison et al., 2011) Overexpression of Rheb[Scer\UAS.cPa] via Scer\GAL4[GMR.PF] causes a hyperplastic phenotype in the eye. (Lee et al., 2010) Expression of Rheb[Scer\UAS.cPa] in the developing eye under the control of Scer\GAL4[GMR.PF] results in a rough eye phenotype with bristle disorganization. (Doumanis et al., 2009) Expression of Rheb^Scer\UAS.cPa driven by Scer\GAL4^ap-md544 in the dorsal epithelial of the wing disc results in enlargement of the dorsal compartment, which causes the wing to curve downwards. When expression of Rheb^Scer\UAS.cPa is driven by Scer\GAL4^ap-md544 in the posterior compartment of the wing, the posterior area is increased but cell density is lower and total cell number is not significantly different to wild type. Therefore, cell size is increased. The anterior compartment of these wings is reduced compared to wild type, while the cell density is increased. (Patel et al., 2003)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Rheb^Scer\UAS.cPa, Scer\GAL4^elav.PLu has neurophysiology defective phenotype, enhanceable by SNF1A^TD.Scer\UAS, Scer\GAL4^elav.PLu (Dimitroff et al., 2012)
Suppressed by
Statement Reference Rheb^Scer\UAS.cPa, Scer\GAL4^elav.PLu has neuroanatomy defective phenotype, suppressible by raptor^GD5138, Scer\GAL4^elav.PLu (Dimitroff et al., 2012) Rheb^Scer\UAS.cPa, Scer\GAL4^elav.PLu has neuroanatomy defective phenotype, suppressible by S6k^GD6646, Scer\GAL4^elav.PLu (Dimitroff et al., 2012) Rheb^Scer\UAS.cPa, Scer\GAL4^elav.PLu has neuroanatomy defective phenotype, suppressible by SNF1A^TD.Scer\UAS, Scer\GAL4^elav.PLu (Dimitroff et al., 2012) Rheb^Scer\UAS.cPa, Scer\GAL4^elav.PLu has neuroanatomy defective phenotype, suppressible by Tor^Δ6B/Tor[+] (Dimitroff et al., 2012) Rheb^Scer\UAS.cPa, Scer\GAL4^elav.PLu has phototaxis defective phenotype, suppressible by SNF1A^TD.Scer\UAS, Scer\GAL4^elav.PLu (Dimitroff et al., 2012) Rheb^Scer\UAS.cPa, Scer\GAL4^elav.PLu has phototaxis defective phenotype, suppressible by Tor^Δ6B/Tor[+] (Dimitroff et al., 2012) Rheb^Scer\UAS.cPa, Scer\GAL4^GMR.PF has visible phenotype, suppressible by Sesn^d04539, Scer\GAL4^GMR.PF (Lee et al., 2010)
NOT suppressed by
Statement Reference Rheb^Scer\UAS.cPa, Scer\GAL4^elav.PLu has neuroanatomy defective phenotype, non-suppressible by rictor^Δ2/rictor^Δ1 (Dimitroff et al., 2012) Rheb^Scer\UAS.cPa, Scer\GAL4^elav.PLu has neuroanatomy defective phenotype, non-suppressible by Sin1^e03756/Sin1^e03756 (Dimitroff et al., 2012)
Enhancer of
Statement Reference Rheb^Scer\UAS.cPa, Scer\GAL4^elav-C155 is an enhancer of neuroanatomy defective phenotype of Hsap\HTT^{98Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1}, Scer\GAL4^elav-C155 (Doumanis et al., 2009)
Suppressor of
Statement Reference Rheb^Scer\UAS.cPa/Scer\GAL4^Tab2-201Y is a suppressor \| partially of neuroanatomy defective \| somatic clone phenotype of Hr51^LL04325 (Yaniv et al., 2012)
Phenotype Manifest In
Enhanced by
Statement Reference Rheb^Scer\UAS.cPa, Scer\GAL4^elav.PLu has embryonic/larval neuromuscular junction phenotype, enhanceable by SNF1A^TD.Scer\UAS, Scer\GAL4^elav.PLu (Dimitroff et al., 2012)
Suppressed by
Statement Reference Rheb^Scer\UAS.cPa, Scer\GAL4^elav.PLu has embryonic/larval neuromuscular junction phenotype, suppressible by rictor^Δ2/rictor^Δ1 (Dimitroff et al., 2012) Rheb^Scer\UAS.cPa, Scer\GAL4^elav.PLu has embryonic/larval neuromuscular junction phenotype, suppressible by Sin1^e03756/Sin1^e03756 (Dimitroff et al., 2012) Rheb^Scer\UAS.cPa, Scer\GAL4^elav.PLu has embryonic/larval neuromuscular junction phenotype, suppressible by Tor^Δ6B/Tor[+] (Dimitroff et al., 2012) Rheb^Scer\UAS.cPa, Scer\GAL4^elav.PLu has photoreceptor cell R7 phenotype, suppressible by raptor^GD5138, Scer\GAL4^elav.PLu (Dimitroff et al., 2012) Rheb^Scer\UAS.cPa, Scer\GAL4^elav.PLu has photoreceptor cell R7 phenotype, suppressible by S6k^GD6646, Scer\GAL4^elav.PLu (Dimitroff et al., 2012) Rheb^Scer\UAS.cPa, Scer\GAL4^elav.PLu has photoreceptor cell R7 phenotype, suppressible by SNF1A^TD.Scer\UAS, Scer\GAL4^elav.PLu (Dimitroff et al., 2012) Rheb^Scer\UAS.cPa, Scer\GAL4^elav.PLu has photoreceptor cell R7 phenotype, suppressible by Tor^Δ6B/Tor[+] (Dimitroff et al., 2012) Rheb^Scer\UAS.cPa, Scer\GAL4^elav.PLu has photoreceptor cell R8 phenotype, suppressible by raptor^GD5138, Scer\GAL4^elav.PLu (Dimitroff et al., 2012) Rheb^Scer\UAS.cPa, Scer\GAL4^elav.PLu has photoreceptor cell R8 phenotype, suppressible by S6k^GD6646, Scer\GAL4^elav.PLu (Dimitroff et al., 2012) Rheb^Scer\UAS.cPa, Scer\GAL4^elav.PLu has photoreceptor cell R8 phenotype, suppressible by SNF1A^TD.Scer\UAS, Scer\GAL4^elav.PLu (Dimitroff et al., 2012) Rheb^Scer\UAS.cPa, Scer\GAL4^elav.PLu has photoreceptor cell R8 phenotype, suppressible by Tor^Δ6B/Tor[+] (Dimitroff et al., 2012) Rheb^Scer\UAS.cPa, Scer\GAL4^GMR.PF has eye phenotype, suppressible by Sesn^d04539, Scer\GAL4^GMR.PF (Lee et al., 2010)
NOT suppressed by
Statement Reference Rheb^Scer\UAS.cPa, Scer\GAL4^elav.PLu has embryonic/larval neuromuscular junction phenotype, non-suppressible by raptor^GD5138, Scer\GAL4^elav.PLu (Dimitroff et al., 2012) Rheb^Scer\UAS.cPa, Scer\GAL4^elav.PLu has embryonic/larval neuromuscular junction phenotype, non-suppressible by S6k^GD6646, Scer\GAL4^elav.PLu (Dimitroff et al., 2012) Rheb^Scer\UAS.cPa, Scer\GAL4^elav.PLu has photoreceptor cell R7 phenotype, non-suppressible by rictor^Δ2/rictor^Δ1 (Dimitroff et al., 2012) Rheb^Scer\UAS.cPa, Scer\GAL4^elav.PLu has photoreceptor cell R7 phenotype, non-suppressible by Sin1^e03756/Sin1^e03756 (Dimitroff et al., 2012) Rheb^Scer\UAS.cPa, Scer\GAL4^elav.PLu has photoreceptor cell R8 phenotype, non-suppressible by rictor^Δ2/rictor^Δ1 (Dimitroff et al., 2012) Rheb^Scer\UAS.cPa, Scer\GAL4^elav.PLu has photoreceptor cell R8 phenotype, non-suppressible by Sin1^e03756/Sin1^e03756 (Dimitroff et al., 2012)
Enhancer of
Statement Reference Scer\GAL4^GMR.PF, Rheb^Scer\UAS.cPa is an enhancer of eye phenotype of Hsap\HTT^{152Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP}, Hsap\HTT^{48Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1}, Scer\GAL4^GMR.PF (Doumanis et al., 2009) Scer\GAL4^GMR.PF, Rheb^Scer\UAS.cPa is an enhancer of pigment cell phenotype of Hsap\HTT^{152Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP}, Hsap\HTT^{48Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1}, Scer\GAL4^GMR.PF (Doumanis et al., 2009)
Suppressor of
Statement Reference Rheb^Scer\UAS.cPa/Scer\GAL4^Tab2-201Y is a suppressor \| partially of gamma Kenyon cell \| somatic clone phenotype of Hr51^LL04325 (Yaniv et al., 2012)
Other
Statement Reference Hr51^LL04325, Rheb^Scer\UAS.cPa, Scer\GAL4^Tab2-201Y has alpha/beta Kenyon cell \| somatic clone phenotype (Yaniv et al., 2012)
Additional Comments
Genetic Interactions
Statement Reference Heterozygosity for Tor[Δ6B] in a Rheb[Scer\UAS.cPa], Scer\GAL4[elav.PLu] background rescues the phototaxis phenotype and photoreceptor axon guidance abnormalities to almost control levels, and causes a significant rescue of the synapse overgrowth phenotype. Co-expression of SNF1A[TD.Scer\UAS] significantly rescues the phototaxis and axon guidance phenotypes, while it enhances the synapse overgrowth and excitatory junctional potential phenotypes, of Rheb[Scer\UAS.cPa], Scer\GAL4[elav.PLu] flies. Co-expression of raptor[GD5138] or S6k[GD6646] significantly rescues the axon guidance phenotypes of Rheb[Scer\UAS.cPa], Scer\GAL4[elav.PLu] flies. The axon guidance phenotypes of Rheb[Scer\UAS.cPa], Scer\GAL4[elav.PLu] flies are not significantly rescued in a rictor[Δ1]/rictor[Δ2] or Sin1[e03756]/Sin1[e03756] background. Co-expression of raptor[GD5138] or S6k[GD6646] does not rescue the Rheb[Scer\UAS.cPa], Scer\GAL4[elav.PLu] synapse overgrowth phenotype. The Rheb[Scer\UAS.cPa], Scer\GAL4[elav.PLu] synapse overgrowth phenotype is significantly rescued in a rictor[Δ1]/rictor[Δ2] or Sin1[e03756]/Sin1[e03756] background. (Dimitroff et al., 2012) Expression of Rheb[Scer\UAS.cPa] in Hr51[LL04325] MARCM neuroblast clones partially suppresses the developmental regrowth defect. In addition these double mutants exhibit severe axon guidance defects. In these clones, γ axons do not occupy the entire adult lobe but instead grow to the dorsal and ventral extremities of the adult γ-lobe and in some cases only to its ventral portion. (Yaniv et al., 2012) Co-expression of Sesn[d04539] with Rheb[Scer\UAS.cPa] via Scer\GAL4[GMR.PF] suppresses the Rheb[Scer\UAS.cPa] hyperplastic eye phenotype. (Lee et al., 2010)
Xenogenetic Interactions
Statement Reference Overexpression of Rheb[Scer\UAS.cPa] in the developing eye enhances the phenotype seen in mutant flies co-expressing Hsap\HTT[152Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP] and Hsap\HTT[48Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1] under the control of Scer\GAL4[GMR.PF], with a severe rough eye phenotype and enhancement of pigment cell loss. Overexpression of Rheb[Scer\UAS.cPa] in the developing eye of Hsap\HTT[18Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1] mutants (both transgene under the control of Scer\GAL4[GMR.PF] results in a rough eye phenotype with bristle disorganization. Co-expression of Rheb[Scer\UAS.cPa] in a Hsap\HTT[98Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1] mutant brain (with both transgenes under the control of Scer\GAL4[elav-C155]) does not significantly increase the average number of brain aggregate inclusions compared to flies expressing Hsap\HTT[98Q.Ex1.Scer\UAS.T:Avic\GFP-EGFP.T:Zzzz\nls1] alone, but does increase the average aggregate size. (Doumanis et al., 2009)
Complementation & Rescue Data
Comments
Stocks ( 2 )
Bloomington	9688 w^; P{UAS-Rheb.Pa}2 9689 w^; P{UAS-Rheb.Pa}3
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	Rheb^Scer\UAS.cPa
Name Synonym	UAS construct a of Patel (Patel et al., 2003)
Secondary FlyBase IDs

References ( 7 )
Research paper	Dimitroff et al., 2012, PLoS ONE 7(2): e30722 Diet and Energy-Sensing Inputs Affect TorC1-Mediated Axon Misrouting but Not TorC2-Directed Synapse Growth in a Drosophila Model of Tuberous Sclerosis. [FBrf0217461] Yaniv et al., 2012, Curr. Biol. 22(19): 1774--1782 Axon Regrowth during Development and Regeneration Following Injury Share Molecular Mechanisms. [FBrf0219661] Eddison et al., 2011, Neuron 70(5): 979--990 arouser Reveals a Role for Synapse Number in the Regulation of Ethanol Sensitivity. [FBrf0213908] Lee et al., 2010, Science 327(5970): 1223--1228 Sestrin as a feedback inhibitor of TOR that prevents age-related pathologies. [FBrf0210161] Doumanis et al., 2009, PLoS ONE 4(9): e7275 RNAi screening in Drosophila cells identifies new modifiers of mutant huntingtin aggregation. [FBrf0209128] Lee et al., 2007, EMBO Rep. 8(4): 360--365 ATG1, an autophagy regulator, inhibits cell growth by negatively regulating S6 kinase. [FBrf0201708] Patel et al., 2003, J. Cell Sci. 116(17): 3601--3610 Drosophila Rheb GTPase is required for cell cycle progression and cell growth. [FBrf0162194]

Allele Dmel\RhebScer\UAS.cPa

Allele Dmel\Rheb^Scer\UAS.cPa