Allele Dmel\PGRP-LE¹¹²

General Information
Symbol	Dmel\PGRP-LE¹¹²	Species	D. melanogaster
Name		FlyBase ID	FBal0182386
Feature type	allele	Associated gene	Dmel\PGRP-LE

Allele class
Mutagen	P-element activity
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	P-element activity (Takehana et al., 2004)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	PGRP-LE^GS1068 (Takehana et al., 2004)
Nature of the lesion	Statement Reference Imprecise excision of the P{GS}PGRP-LE^GS1068 insertion has created a 2542bp deletion which includes the PGRP-LE start codon. (Takehana et al., 2004)
Cytology
Phenotypic Data
Phenotypic Class
	fertile (Takehana et al., 2004) immune response defective (Takehana et al., 2004, Goto et al., 2010, Neyen et al., 2012) viable (Takehana et al., 2004)
Phenotype Manifest In

Detailed Description
	Statement Reference Mutant flies show reduced survival after infection with Erwinia carotovora carotovora by septic injury compared to controls. (Neyen et al., 2012) PGRP-LE[112] flies show lower survival rates after L. monocytogenes infection compared to controls. (Goto et al., 2010) Hemolymph clots from PGRP-LE[112] third instar larvae show normal melanization. (Bidla et al., 2007) The survival rate of mutant animals after bacterial infections is similar to that of wild-type except for infection by P.carotovorum.carotovorum, and Gram-positive bacterial infections, B.megaterium, B.subtilis, E.faecalis and M.luteus. After infection with E.coli weak melanisation is induced at the injury site in mutant animals. (Takehana et al., 2004)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference PGRP-LE¹¹² has immune response defective phenotype, enhanceable by PGRP-LC⁷⁴⁵⁴ (Takehana et al., 2004)
Suppressed by
Statement Reference PGRP-LE¹¹² has immune response defective phenotype, suppressible by Listericin^Scer\UAS.cGa/Scer\GAL4^Cg.PA (Goto et al., 2010)
Enhancer of
Statement Reference PGRP-LE¹¹² is an enhancer of immune response defective phenotype of PGRP-LC⁷⁴⁵⁴ (Takehana et al., 2004)
Suppressor of
Statement Reference PGRP-LE¹¹² is a suppressor of immune response defective phenotype of PGRP-LB^Δ (Bosco-Drayon et al., 2012) PGRP-LE¹¹² is a suppressor of partially lethal - majority die phenotype of PGRP-LF²⁰⁰ (Maillet et al., 2008)
NOT Suppressor of
Statement Reference PGRP-LE¹¹² is a non-suppressor of visible \| adult stage phenotype of PGRP-LF²⁰⁰ (Maillet et al., 2008)
Other
Statement Reference PGRP-LC^ΔE, PGRP-LE¹¹² has immune response defective phenotype (Aggarwal et al., 2008)
Phenotype Manifest In
NOT Suppressor of
Statement Reference PGRP-LE¹¹² is a non-suppressor of wing phenotype of PGRP-LF²⁰⁰ (Maillet et al., 2008)
Additional Comments
Genetic Interactions
Statement Reference The reduced survival of PGRP-LB[Δ] flies after infection with Erwinia carotovora carotovora is suppressed if they are also mutant for PGRP-LE[112]. (Bosco-Drayon et al., 2012) Expression of Listericin[Scer\UAS.cGa] using Scer\GAL4[Cg.PA] restores the survival phenotype against L. monocytogenes infection in a PGRP-LE[112] background. (Goto et al., 2010) PGRP-LE[112];PGRP-LC[ΔE] double mutant flies are rapidly killed by infection with the gram-negative pathogen Erwinia carotovora carotovora. (Aggarwal et al., 2008) PGRP-LE[112] does not suppress the notching phenotypes and low adult viability seen in homozygous PGRP-LF[200] mutants. (Maillet et al., 2008) Unlike single mutants, PGRP-LC⁷⁴⁵⁴/PGRP-LE¹¹² double mutant animals exhibit a reduced resistance to E.coli and B.megaterium infection, but not E.faecalis and M.luteus infection. (Takehana et al., 2004)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 1 )
Bloomington	33055 y¹ w^67c23 PGRP-LE¹¹²
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	LE¹¹² (Goto et al., 2010) PGRP-LE¹¹² (Tsuzuki et al., 2012, Davis et al., 2008, Neyen et al., 2012, Aggarwal et al., 2008, Goto et al., 2010, Bidla et al., 2007, Maillet et al., 2008, Bosco-Drayon et al., 2012)
Name Synonym
Secondary FlyBase IDs

References ( 9 )
Research paper	Bosco-Drayon et al., 2012, Cell Host Microbe 12(2): 153--165 Peptidoglycan Sensing by the Receptor PGRP-LE in the Drosophila Gut Induces Immune Responses to Infectious Bacteria and Tolerance to Microbiota. [FBrf0219236] Neyen et al., 2012, J. Immunol. 189(4): 1886--1897 Tissue- and Ligand-Specific Sensing of Gram-Negative Infection in Drosophila by PGRP-LC Isoforms and PGRP-LE. [FBrf0219094] Tsuzuki et al., 2012, Sci. Rep. 2: 210 Drosophila growth-blocking peptide-like factor mediates acute immune reactions during infectious and non-infectious stress. [FBrf0217467] Goto et al., 2010, J. Biol. Chem. 285(21): 15731--15738 Cooperative regulation of the induction of the novel antibacterial Listericin by peptidoglycan recognition protein LE and the JAK-STAT pathway. [FBrf0210770] Aggarwal et al., 2008, PLoS Pathog. 4(8): e1000120 Rudra interrupts receptor signaling complexes to negatively regulate the IMD pathway. [FBrf0205806] Davis et al., 2008, Mol. Cell. Biol. 28(15): 4883--4895 A member of the p38 mitogen-activated protein kinase family is responsible for transcriptional induction of Dopa decarboxylase in the epidermis of Drosophila melanogaster during the innate immune response. [FBrf0215839] Maillet et al., 2008, Cell Host Microbe 3(5): 293--303 The Drosophila peptidoglycan recognition protein PGRP-LF blocks PGRP-LC and IMD/JNK pathway activation. [FBrf0204914] Bidla et al., 2007, J. Cell Sci. 120(7): 1209--1215 Crystal cell rupture after injury in Drosophila requires the JNK pathway, small GTPases and the TNF homolog Eiger. [FBrf0192653] Takehana et al., 2004, EMBO J. 23(23): 4690--4700 Peptidoglycan recognition protein (PGRP)-LE and PGRP-LC act synergistically in Drosophila immunity. [FBrf0180202]

Allele Dmel\PGRP-LE112

Allele Dmel\PGRP-LE¹¹²