Allele Dmel\Nedd4^T119FS

General Information
Symbol	Dmel\Nedd4^T119FS	Species	D. melanogaster
Name		FlyBase ID	FBal0182538
Feature type	allele	Associated gene	Dmel\Nedd4

Map ( GBrowse )
Allele class
Mutagen	ethyl methanesulfonate
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	ethyl methanesulfonate (Sakata, 2004)
Mutations Mapped to the Genome

Type Location Additional Notes References complex substitution 3L:17,532,556..17,532,558 evidence=experimental comment=Frameshift at codon 119. The P{GSV6}Nedd4^GS13178 insertion is still present on the chromosome. (Sakata et al., 2004)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	P{GSV6}Nedd4^GS13178 (Sakata, 2004)
Nature of the lesion	Statement Reference Frameshift at position T119. (Sakata et al., 2004) Amino acid alteration. In addition, the P{GSV6}Nedd4^GS13178 insertion is still present on the chromosome. (Sakata, 2004)
Caused by insertion	P{GSV6}Nedd4^GS13178 (Sakata, 2004)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| embryonic stage \| recessive (Zhong et al., 2011) viable, with Scer\GAL4^da.G32 (Sakata, 2004)
Phenotype Manifest In

Detailed Description
	Statement Reference Homozygous clones in the wing and leg discs survive without significant developmental defects. (Sakata et al., 2004)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement Reference Scer\GAL4[-]/Nedd4^T119FS is a suppressor of visible \| dominant phenotype of N^264-39 (Sakata et al., 2004)
Phenotype Manifest In
Enhancer of
Statement Reference Nedd4[+]/Nedd4^T119FS is an enhancer of wing phenotype of Scer\GAL4^da.G32, Sep4^Scer\UAS.cMa (Munoz-Soriano et al., 2012)
Suppressor of
Statement Reference Scer\GAL4[-]/Nedd4^T119FS is a suppressor of wing margin phenotype of N^264-39 (Sakata et al., 2004)
Additional Comments
Genetic Interactions
Statement Reference Heterozygosity for Nedd4[T119FS] enhances the wing phenotype caused by expression of two copies of Sep4[Scer\UAS.cMa] under the control of Scer\GAL4[da.G32]. (Munoz-Soriano et al., 2012) The nicked wing phenotype seen in N^264-39/+ flies is suppressed by Nedd4^T119FS/+. (Sakata et al., 2004)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Partially rescued by	Nedd4^T119FS is partially rescued by Nedd4^{Scer\UAS.Short.T:Zzzz\FLAG}/Scer\GAL4^Act5C.PI (Zhong et al., 2011)
Not rescued by	Nedd4^T119FS is not rescued by Scer\GAL4^Act5C.PI/Nedd4^{Scer\UAS.Long.T:Zzzz\FLAG} (Zhong et al., 2011)
Comments	Expression of Nedd4[Scer\UAS.Long.T:Zzzz\FLAG] throughout the embryo driven by Scer\GAL4[Act5C.PI] fails to rescue the lethality of homozygous Nedd4[T119FS] mutant embryos. Expression of Nedd4[Scer\UAS.Short.T:Zzzz\FLAG] throughout the embryo driven by Scer\GAL4[Act5C.PI] partially rescues the lethality of homozygous Nedd4[T119FS] mutant embryos. (Zhong et al., 2011)
Stocks ( 1 )
Kyoto	109169 y¹ w^67c23; Nedd4^T119FS P{FRT(w^hs)}2A / TM6B
Notes on Origin
Discoverer
	Selected as: a revertant of the larval lethality caused by expression of Nedd4^GS13178 under the control of Scer\GAL4^da.G32. (Sakata, 2004)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 4 )
Reported As
Symbol Synonym	dNedd4^T121FS (Zhong et al., 2011) Nedd4^T119FS nedd4^T119FS (Munoz-Soriano et al., 2012) unnamed (Sakata, 2004)
Name Synonym
Secondary FlyBase IDs

References ( 4 )
Research paper	Munoz-Soriano et al., 2012, J. Mol. Neurosci. 48(1): 136--143 Septin 4, the Drosophila Ortholog of Human CDCrel-1, Accumulates in parkin Mutant Brains and is Functionally Related to the Nedd4 E3 Ubiquitin Ligase. [FBrf0219099] Zhong et al., 2011, PLoS ONE 6(11): e27007 A Splice Isoform of DNedd4, DNedd4-Long, Negatively Regulates Neuromuscular Synaptogenesis and Viability in Drosophila. [FBrf0216790] Sakata et al., 2004, Curr. Biol. 14(24): 2228--2236 Drosophila Nedd4 regulates endocytosis of Notch and suppresses its ligand-independent activation. [FBrf0183817]
Supplementary material	Sakata, 2004, Curr. Biol. 14(24): Supplemental Data. [FBrf0188485]

Allele Dmel\Nedd4T119FS

Allele Dmel\Nedd4^T119FS