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General Information
Symbol
Dmel\MbsN300.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0182792
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-MbsN300, UAS-mbsN300
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Expression of a constitutively active form of Mbs, which consists of the N-terminal 300 amino acids, is governed by UASt regulatory sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Stage 16 MbsN300.Scer\UAS embryos under the control of the Scer\GAL4how-24B driver show laterality defects in the proventriculus and anterior midgut. The laterality of the other parts of the embryonic gut, including the hindgut and the posterior part of the midgut is normal in these mutants. The proventriculus and the anterior midgut do not rotate (as in wild-type) in these mutants at stages 15 to 17.

Stage 16 MbsN300.Scer\UAS embryos under the control of either Scer\GAL4NP5021 or Scer\GAL4NP221 do not show any loss of rotation in the proventriculus and anterior midgut.

Expression of MbsN300.Scer\UAS in larval epidermal cells, under the control of Scer\GAL4NP0056, results in a significant proportion of these cells failing to extrude and remaining within the abdominal epidermis at postmetamorphosis periods. This results in cuticular clefts in the adult abdomen.

Expression of MbsN300.Scer\UAS, under the control of Scer\GAL4da.G32, causes lethality at or before the pupal stage. When its expression is driven in the eye disc by Scer\GAL4ey.PH or Scer\GAL4GMR.PF, there are no apparent defects in eye receptor differentiation.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhancer of
Statement
Reference

MbsN300.UAS/Scer\GAL4GMR.PU is an enhancer of retina phenotype of eys1, prom[+]/prom1

Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference

Expression of MbsN300.Scer\UAS under the control of Scer\GAL4GMR.PU enhances the rhabdomere fusion seen in eys1 prom1 double heterozygotes.

Overexpression of both rokCAT.Scer\UAS and MbsN300.Scer\UAS, under the control of Scer\GAL4da.G32, allow ~30% of flies to survive to adulthood, instead of dying as pupae.

Xenogenetic Interactions
Statement
Reference

Co-expression of MbsN300.Scer\UAS and BacA\p35Scer\UAS.cHa in the dorsal compartment of the wing disc (under the temperature-regulated control of Scer\GAL4ap-md544 by Scer\GAL80ts.αTub84B) does not alter apical-basal cell length in mutant cells compared with control cells.

The shorter cell length observed in wing disc tissue expressing DadScer\UAS.cTa and BacA\p35Scer\UAS.cHa (under the temperature-regulated control of Scer\GAL4ap-md544 by Scer\GAL80ts.αTub84B), is rescued by co-expression of MbsN300.Scer\UAS.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
Comments
Comments

Encodes a constitutively activated form of Mbs.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
MbsN300.UAS
Name Synonyms
Secondary FlyBase IDs
    References (10)