Allele Dmel\Klp67A^322b24

General Information
Symbol	Dmel\Klp67A^322b24	Species	D. melanogaster
Name		FlyBase ID	FBal0183014
Feature type	allele	Associated gene	Dmel\Klp67A

Allele class
Mutagen	Delta2-3
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	Delta2-3 (Gandhi et al., 2004)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	P{EP}Klp67A^EP3516 (Gandhi et al., 2004)
Nature of the lesion	Statement Reference Local hopping of the P{EP} element insertion, causing a small deletion of <35bp within the promoter region of Klp67A. This causes transcription to begin at the P{EP} element instead of at the normal transcription initiation site. (Gandhi et al., 2004)
Caused by insertion	P{EP}Klp67A^322b24 (Gandhi et al., 2004)
Cytology
Phenotypic Data
Phenotypic Class
	cytokinesis defective \| male (with Df(3L)29A6) (Gandhi et al., 2004) female semi-sterile (with Df(3L)29A6) (Gandhi et al., 2004, Wang et al., 2010) fertile (with Klp67A^{Ubi-p63E.T:Avic\GFP}), with Df(3L)29A6 (Wang et al., 2010, Wang et al., 2010) male sterile (with Df(3L)29A6) (Gandhi et al., 2004, Wang et al., 2010) meiotic cell cycle defective \| male (with Df(3L)29A6) (Gandhi et al., 2004) mitotic cell cycle defective (with Df(3L)29A6) (Gandhi et al., 2004)
Phenotype Manifest In
	centrosome \| blastoderm stage \| maternal effect (with Df(3L)29A6) (Gandhi et al., 2004) meiosis I \| male (with Df(3L)29A6) (Gandhi et al., 2004) meiosis II \| male (with Df(3L)29A6) (Gandhi et al., 2004) mitotic anaphase B \| blastoderm stage \| maternal effect (with Df(3L)29A6) (Gandhi et al., 2004) mitotic metaphase \| blastoderm stage \| maternal effect (with Df(3L)29A6) (Gandhi et al., 2004) spermatid & Nebenkern (with Df(3L)29A6) (Gandhi et al., 2004) spermatid & nucleus (with Df(3L)29A6) (Gandhi et al., 2004) spermatocyte & aster \| ectopic (with Df(3L)29A6) (Gandhi et al., 2004) spermatocyte & astral microtubule (with Df(3L)29A6) (Gandhi et al., 2004) spermatocyte & centrosome \| supernumerary (with Df(3L)29A6) (Gandhi et al., 2004) spermatocyte & condensed nuclear chromosome (with Df(3L)29A6) (Gandhi et al., 2004) spermatocyte & contractile ring (with Df(3L)29A6) (Gandhi et al., 2004) spermatocyte & spindle \| supernumerary (with Df(3L)29A6) (Gandhi et al., 2004) spindle \| blastoderm stage \| maternal effect (with Df(3L)29A6) (Gandhi et al., 2004) spindle \| embryonic stage 4 (with Df(3L)29A6) (Wang et al., 2010) spindle microtubule \| blastoderm stage \| maternal effect (with Df(3L)29A6) (Gandhi et al., 2004)
Detailed Description
	Statement Reference Klp67A[322b24]/Df(3L)29A6 flies are male sterile and female semi-sterile. Klp67A[322b24]/Df(3L)29A6 mutant syncytial embryos display premature mitotic spindle elongation. Klp67A[Ubi-p63E.T:Avic\GFP] restores fertility and viability to Klp67A[322b24]/Df(3L)29A6 mutants. The major mitotic defect caused by depletion of Klp67A in the mutant embryos is rescued by Klp67A[Ubi-p63E.T:Avic\GFP]. Klp67A[Ubi-p63E.T:Avic\GFP] restores wild-type spindle morphology and geometry and the dynamics of mitotic spindle poles in the rescued flies is virtually identical to that seen in wild-type embryos. (Wang et al., 2010) Klp67A^322b24/Df(3L)29A6 mutant spermatocytes show defects throughout meiosis. In late prophase I, and to a lesser extent in late prophase II, asters ectopically localize close together in the cytoplasm, instead of at opposite sides of the nucleus. Despite this defect, the majority of primary spermatocytes assemble a bipolar spindle, form a metaphase plate and proceed through anaphase. The spindles of anaphase and metaphase I and II have astral microtubules that are longer than wild type and show abnormal chromosome segregation. In telophase I and II the astral microtubules are even longer and the central spindle is often absent or much less dense than in wild type. A minority of spermatocytes in anaphase I and II have two nuclei of different sizes. Almost half of the cells in ana-telophase II contain two spindles within the same cytoplasm, suggesting cytokinesis failure during the first meiotic division. These cells also have long astral microtubules that overlap, causing highly disorganized spindle architecture. Spermatocytes in telophase I with a normal central spindle display a regular contractile ring; those with a poorly organized central spindle appear unable to assemble a contractile ring. Klp67A^322b24/Df(3L)29A6 mutant spermatids often have an unusually large nebenkern associated with two or four nuclei, consistent with a failure in meiotic cytokinesis. Additionally, some spermatids contain nuclei of irregular sizes, consistent with defects in chromosome segregation during meiosis. Some mutants have polyploid spermatocytes with four not two centrosomes, suggesting that Klp67A is involved with cytokinesis in the gonial mitoses that precede meiotic division. Blastoderm embryos from Klp67A^322b24/Df(3L)29A6 mothers show abnormal spindle formation and architecture throughout mitosis. During prophase, most centrosomes do not complete migration to opposite sides of the nucleus and the period from centrosome migration to nuclear breakdown is longer than in wild-type embryos. The incomplete centrosome separation causes spindles to become distorted and banana-shaped to accommodate extended microtubules from spindle poles. In some metaphase spindles, centrosomes detach from the spindle poles. Additionally, the metaphase spindle is increased in length, whether centrosome separation is normal or not. The interval between nuclear envelope breakdown and the appearance of a central spindle is longer than wild type due to an increase in time spent in metaphase and anaphase B. Unlike in wild type, mutant spindles continue to increase in length during metaphase and pole separation occurs later in anaphase B. During telophase, most spindles are missing a normal central spindle or have a greatly reduced number of midzone microtubules, which are not organized in the typical dense lateral array seen in wild type. Following telophase, two daughter spindles can form during ensuing divisions. Despite the above defects, chromosome segregation appears to proceed as in wild type. (Gandhi et al., 2004)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	Df(3L)29A6/Klp67A^322b24 is rescued by Klp67A^{Ubi-p63E.T:Avic\GFP} (Wang et al., 2010) Df(3L)29A6/Klp67A^322b24 is rescued by Klp67A^{Ubi-p63E.T:Hsap\MYC} (Gandhi et al., 2004)
Comments	Klp67A[Ubi-p63E.T:Avic\GFP] restores fertility and viability to Klp67A[322b24]/Df(3L)29A6 mutants. The major mitotic defect caused by depletion of Klp67A in the mutant embryos is rescued by Klp67A[Ubi-p63E.T:Avic\GFP]. Klp67A[Ubi-p63E.T:Avic\GFP] restores wild-type spindle morphology and geometry and the dynamics of mitotic spindle poles in the rescued flies is virtually identical to that seen in wild-type embryos. (Wang et al., 2010)
Stocks ( 1 )
Bloomington	35507 w^*; P{EP}Klp67A^322b24/TM6B, Tb¹
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	Klp67A^322b24 Klp67A^332b24 (Wang et al., 2010)
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Wang et al., 2010, Cytoskeleton (Hoboken) 67(11): 715--728 Coupling between microtubule sliding, plus-end growth and spindle length revealed by kinesin-8 depletion. [FBrf0212142] Gandhi et al., 2004, Mol. Biol. Cell 15(1): 121--131 The Drosophila kinesin-like protein KLP67A is essential for mitotic and male meiotic spindle assembly. [FBrf0167863]

Allele Dmel\Klp67A322b24

Allele Dmel\Klp67A^322b24