FlyBase Allele Report: Hsap\APP[UAS.Tag:MYC]

General Information

Symbol

Hsap\APP^UAS.Tag:MYC

Species

H. sapiens

Name

FlyBase ID

FBal0183229

Feature type

allele

Associated gene

Hsap\APP

Associated Insertion(s)

Carried in Construct

P{UAS-Hsap\APP.T:Myc}

Also Known As

UAS-APP

Key Links

Transgenic product class

wild_type

(Merdes et al., 2004)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Merdes et al., 2004)

Mutagen

in vitro construct

(Merdes et al., 2004)

Progenitor genotype

Carried in construct

P{UAS-Hsap\APP.T:Myc}

Cytology

Description

UAS sequences drive expression of Hsap\APP tagged with Tag:MYC.

(Merdes et al., 2004)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Hsap\APP

Tagged with

Tag:MYC

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Alzheimer's disease

CEA

(Rusu et al., 2007)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^P2.4.Pdf

(Leyssen et al., 2005)

abnormal neurophysiology, with Scer\GAL4^179Y

(Rusu et al., 2007)

abnormal neurophysiology, with Scer\GAL4^elav-C155

(Rusu et al., 2007)

Phenotype Manifest In

mechanosensory sensory organ, with Scer\GAL4^sca-537.4

(Merdes et al., 2004)

transport vesicle, with Scer\GAL4^179Y

(Rusu et al., 2007)

transport vesicle, with Scer\GAL4^elav-C155

(Rusu et al., 2007)

ventral adult lateral neuron & axon, with Scer\GAL4^P2.4.Pdf

(Leyssen et al., 2005)

Detailed Description

Statement

Reference

The axons of larvae expressing Hsap\APP^{Scer\UAS.T:Hsap\MYC} (under the control of either Scer\GAL4^elav-C155 or Scer\GAL4^179Y) exhibit vesicle accumulation, indicating a defect in axonal transport.

The amplitude of basal EJC and spontaneous mEJPs is unchanged in larvae that express Hsap\APP^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^179Y or Scer\GAL4^elav-C155 compared to wild-type.

Post-tetanic potentiation (PTP) is enhanced in axons from nerves expressing Hsap\APP^{Scer\UAS.T:Hsap\MYC} (under the control of either Scer\GAL4^elav-C155 or Scer\GAL4^179Y); after the early PTP, their EJC amplitude folows the common decay for approximately 1 minute but then remains relatively stable at approximately 115% of the baseline level.

(Rusu et al., 2007)

Expressing Hsap\APP^{Scer\UAS.T:Hsap\MYC}, under the control of Scer\GAL4^P2.4.Pdf, has no effect on the early development of the axonal pattern of sLNv neurons. However, Scer\GAL4^P2.4.Pdf>Hsap\APP^{Scer\UAS.T:Hsap\MYC} has a largely postdevelopmental effect on the neurite arborization of the Drosophila brain. Scer\GAL4^P2.4.Pdf>Hsap\APP^{Scer\UAS.T:Hsap\MYC} expression results in a large increase in axon outgrowth and arborization of the sLNv. This effect is greater at 28^oC than at 18 or 25^oC, which corresponds to higher levels of Scer\GAL4^P2.4.Pdf-driven Hsap\APP^{Scer\UAS.T:Hsap\MYC} expression at higher temperatures.

(Leyssen et al., 2005)

When Hsap\APP^{Scer\UAS.T:Hsap\MYC} is driven by Scer\GAL4^sca-537.4, a phenotype in the mechanosensory organs is seen.

(Merdes et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Hsap\APP^UAS.Tag:MYC, Scer\GAL4^P2.4.Pdf has abnormal neuroanatomy phenotype, suppressible by Abl¹/Abl[+]

(Leyssen et al., 2005)

Hsap\APP^UAS.Tag:MYC, Scer\GAL4^P2.4.Pdf has abnormal neuroanatomy phenotype, suppressible by Abl⁴/Abl[+]

(Leyssen et al., 2005)

Hsap\APP^UAS.Tag:MYC, Scer\GAL4^P2.4.Pdf has abnormal neuroanatomy phenotype, suppressible by Abl^K417N.UAS

(Leyssen et al., 2005)

Hsap\APP^UAS.Tag:MYC, Scer\GAL4^P2.4.Pdf has abnormal neuroanatomy phenotype, suppressible by bsk^DN.UAS

(Leyssen et al., 2005)

Hsap\APP^UAS.Tag:MYC, Scer\GAL4^P2.4.Pdf has abnormal neuroanatomy phenotype, suppressible by chic[+]/chic⁰¹³²⁰

(Leyssen et al., 2005)

Hsap\APP^UAS.Tag:MYC, Scer\GAL4^P2.4.Pdf has abnormal neuroanatomy phenotype, suppressible by chic²²¹

(Leyssen et al., 2005)

Hsap\APP^UAS.Tag:MYC, Scer\GAL4^P2.4.Pdf has abnormal neuroanatomy phenotype, suppressible by kay¹/kay[+]

(Leyssen et al., 2005)

Hsap\APP^UAS.Tag:MYC, Scer\GAL4^P2.4.Pdf has abnormal neuroanatomy phenotype, suppressible | partially by Jra^IA109/Jra[+]

(Leyssen et al., 2005)

NOT suppressed by

Statement

Reference

Hsap\APP^UAS.Tag:MYC, Scer\GAL4^P2.4.Pdf has abnormal neuroanatomy phenotype, non-suppressible by ena⁰²⁰²⁹/ena[+]

(Leyssen et al., 2005)

Hsap\APP^UAS.Tag:MYC, Scer\GAL4^P2.4.Pdf has abnormal neuroanatomy phenotype, non-suppressible by tsr[+]/tsr^k05633

(Leyssen et al., 2005)

Phenotype Manifest In

Enhanced by

Statement

Reference

Hsap\APP^UAS.Tag:MYC, Scer\GAL4^sca-537.4 has mechanosensory sensory organ phenotype, enhanceable by H^P8

(Merdes et al., 2004)

Hsap\APP^UAS.Tag:MYC, Scer\GAL4^sca-537.4 has mechanosensory sensory organ phenotype, enhanceable by numb¹⁵

(Merdes et al., 2004)

Suppressed by

Statement

Reference

Hsap\APP^UAS.Tag:MYC, Scer\GAL4^P2.4.Pdf has ventral adult lateral neuron & axon phenotype, suppressible by kay¹/kay[+]

(Leyssen et al., 2005)

Hsap\APP^UAS.Tag:MYC, Scer\GAL4^P2.4.Pdf has ventral adult lateral neuron & axon phenotype, suppressible | partially by Jra^IA109/Jra[+]

(Leyssen et al., 2005)

Hsap\APP^UAS.Tag:MYC, Scer\GAL4^P2.4.Pdf has ventral adult lateral neuron & axon phenotype, suppressible by Abl¹/Abl[+]

(Leyssen et al., 2005)

Hsap\APP^UAS.Tag:MYC, Scer\GAL4^P2.4.Pdf has ventral adult lateral neuron & axon phenotype, suppressible by Abl⁴/Abl[+]

(Leyssen et al., 2005)

Hsap\APP^UAS.Tag:MYC, Scer\GAL4^P2.4.Pdf has ventral adult lateral neuron & axon phenotype, suppressible by Abl^K417N.UAS

(Leyssen et al., 2005)

Hsap\APP^UAS.Tag:MYC, Scer\GAL4^P2.4.Pdf has ventral adult lateral neuron & axon phenotype, suppressible by bsk^DN.UAS

(Leyssen et al., 2005)

Hsap\APP^UAS.Tag:MYC, Scer\GAL4^P2.4.Pdf has ventral adult lateral neuron & axon phenotype, suppressible by chic[+]/chic⁰¹³²⁰

(Leyssen et al., 2005)

Hsap\APP^UAS.Tag:MYC, Scer\GAL4^P2.4.Pdf has ventral adult lateral neuron & axon phenotype, suppressible by chic²²¹

(Leyssen et al., 2005)

Hsap\APP^UAS.Tag:MYC, Scer\GAL4^sca-537.4 has mechanosensory sensory organ phenotype, suppressible by N^55e11

(Merdes et al., 2004)

NOT suppressed by

Statement

Reference

Hsap\APP^UAS.Tag:MYC, Scer\GAL4^P2.4.Pdf has ventral adult lateral neuron & axon phenotype, non-suppressible by ena⁰²⁰²⁹/ena[+]

(Leyssen et al., 2005)

Hsap\APP^UAS.Tag:MYC, Scer\GAL4^P2.4.Pdf has ventral adult lateral neuron & axon phenotype, non-suppressible by tsr[+]/tsr^k05633

(Leyssen et al., 2005)

Enhancer of

Statement

Reference

Hsap\APP^UAS.Tag:MYC, Scer\GAL4^sca-537.4 is an enhancer of mechanosensory sensory organ phenotype of Hsap\APLP2::Hsap\APP^UAS.Tag:MYC, Scer\GAL4^sca-537.4

(Merdes et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Heterozygosity for the following alleles suppresses the Scer\GAL4^P2.4.Pdf>Hsap\APP^{Scer\UAS.T:Hsap\MYC}-induced increase in sLNv axonal arborization: Abl¹, Abl⁴, chic⁰¹³²⁰, chic²²¹ and kay¹. Jra^IA109/+ partially suppresses this phenotype. Coexpression of Abl^{K417N.Scer\UAS} or bsk^DN.Scer\UAS in the sLNv also reduces in the Hsap\APP^{Scer\UAS.T:Hsap\MYC}-induced axonal arborization. Heterozygosity for kay¹ or ena⁰²⁰²⁹ does not affect the arborization phenotype.

(Leyssen et al., 2005)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Transfected into S2 cells to study the effect of the expressed protein on cell clustering.

(Soba et al., 2005)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Hsap\APP^{Scer\UAS.T:Hsap\MYC}

Hsap\APP^UAS.Tag:MYC

Name Synonyms

Secondary FlyBase IDs

References (4)

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