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General Information
Symbol
Dmel\GluRIIAhypo.tQa
Species
D. melanogaster
Name
FlyBase ID
FBal0183419
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
GluRIIAhypo
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

A genomic fragment that encompasses the promoter region and the whole open reading frame of GluRIIA but lacks most of the 3' UTR.

Allele components
Product class / Tool use(s)
Regulatory region(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of GluRIIAhypo.Scer\UAS under the control of Scer\GAL4RapGAP1-OK6 in a GluRIIA GluRIIB double mutant background (through Df(2L)cl-h4/Df(2L)SP22) results in larvae that are only moderately limited in mobility. Synaptic active zones appear to form correctly in these mutants.

Flies expressing GluRIIAhypo.Scer\UAS under the control of Scer\GAL4RapGAP1-OK6 (in a Df(2L)cl-h4/Df(2L)SP22, GluRIIA GluRIIB double mutant background) exhibit a chronic increase in presynaptic release, as shown by paired-pulse stimulation of the synaptic junction leading to an atypical depression. In addition, the decay time constant of evoked responses is increased in these mutants. The rise time of evoked junctional currents is not significantly altered.

Flies expressing GluRIIAhypo.Scer\UAS under the control of Scer\GAL4RapGAP1-OK6 (in a Df(2L)cl-h4/Df(2L)SP22, GluRIIA GluRIIB double mutant background) exhibit a reduction in both the number of synapses per neuromuscular junction and the number of boutons in each synapse. In addition, some boutons appear abnormally round. Within neuromuscular junctions of mature larvae, synaptic vesicles and typical organelles such as mitochondria, seem unaffected. Moreover, presynaptic T-bars are found at apparently normal frequency, consistent with the preservation of active zone function.

The synapses of flies expressing GluRIIAhypo.Scer\UAS under the control of Scer\GAL4RapGAP1-OK6 (in a Df(2L)cl-h4/Df(2L)SP22, GluRIIA GluRIIB double mutant background) exhibit severe defects in membrane organisation. Normally, presynaptic and postsynaptic membranes are more electron dense than neighboring extra-synaptic membranes, and show a flat and linear apposition. Neuromuscular junctions in these mutants show both presynaptic and postsynaptic membranes with either no or reduced electron density. Most notably, the area of close apposition between presynaptic and postsynaptic membrane is clearly reduced or sometimes totally absent at these synapses.

Expression of GluRIIAhypo.Scer\UAS under the control of Scer\GAL4elav-C155 rescues approximately 43% of expected mendelian rate of adult flies in a Df(2L)cl-h4 (GluRIIA GluRIIB double mutant).

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

In a GluRIIB null background (Df(2L)SP22/Df(2L)cl-h4), GluRIIAhypo.Scer\UAS larvae show clear signs of paralysis. Spontaneous mEJCs are below the detection limit, indicating an extreme drop in postsynaptic glutamate receptor function.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
GluRIIAhypo.Scer\UAS
GluRIIAhypo.tQa
GluRIIAhypo
Name Synonyms
Secondary FlyBase IDs
    References (2)