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General Information
Symbol
Dmel\GαoGTP.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0183487
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-Go-GTP
Allele class
Mutagen
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference
The FRT cassette has been excised, juxtaposing the UAS regulatory sequences and mutant form of the Gαo class II isoform. Encodes a form of Gαo which is maintained in the GTP bound state.
Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
Scer\GAL4Bx-MS1096-mediated expression of GαoGTP.Scer\UAS results in clearly visible defects in stout sensory bristle formation on the adult wing margin.
Approximately 50% of flies expressing constitutively active G-oα47AGTP.Scer\UAS in LN[[v]] neurons under the control of Scer\GAL4P0.5.Pdf are arrhythmic The remaining rhythmic flies have short period rhythms (22.3hr), although with a weak power.
The response of the ab1C neuron to CO[[2]] is unaffected in animals expressing G-oα47AGTP.Scer\UAS under the control of Scer\GAL4Gr21a.9.323. The odour responses of the ab1A, ab2A and ab3A olfactory receptor neurons are unaffected in animals expressing G-oα47AGTP.Scer\UAS under the control of Scer\GAL4Orco.T:Hsim\VP16.
Expression of G-oα47AGTP.Scer\UAS, under the control of Scer\GAL4pnr-MD237 results in bristle orientation defects and bald regions of the thorax. Scer\GAL4pnr-MD237 drives expression of G-oα47AGTP.Scer\UAS in eight thoracic macrochaetae, and on average, 15% of these macrochaetae are lost per thorax, with all thoraces exhibiting loss of at least one macrochaeta. Defects, due to aberrations in asymmetric division, including the absence of external cells, duplicated sockets and/or hairs, and bristles devoid of sockets or hairs are observed in G-oα47AGTP.Scer\UAS Scer\GAL4pnr-MD237 mutants. Approximately 8% of G-oα47AGTP.Scer\UAS Scer\GAL4pnr-MD237 bristles exhibit hair/socket duplications. Socket-only phenotypes occur in 28% of cases. Flies expressing G-oα47AGTP.Scer\UAS under the control of Scer\GAL4pnr-MD237 exhibit defective wing margin stout bristles. Defective bristles exhibit a range of phenotypes, they may contain: two sockets/two hairs, one socket/three hairs, one socket/two hairs, two sockets/one hair, no socket/two hairs, no socket/one hair, or one or two sockets/no hair. Flies expressing G-oα47AGTP.Scer\UAS under the control of Scer\GAL4Bx-MS1096 exhibit defects in approximately 3% of wing margin bristles. In flies expressing G-oα47AGTP.Scer\UAS under the control of Scer\GAL4sca-537.4, the internal cells of sensory organ precursors (SOPs) can duplicate at the expense of external cells, forming two neuronal and two sheath cells, in approximately 10% of bristles. In approximately 1% of cases, four neuronal cells are formed. This is consistent with the bald regions observed on the adult thorax in this genotype, resulting from pIIa-to-pIIb transformations. External cells can also be duplicated in this genotype. In approximately 4% of cases, the SOP cluster duplicates the socket cells at the expense of neuronal specification.
Expression of G-oα47AGTP.Scer\UAS under the control of Scer\GAL4Bx-MS1096 induces multiple wing hairs. This phenotype is cell autonomous.
Expression of G-oα47AGTP.Scer\UAS in glia of embryos, driven by Scer\GAL4repo, causes defects in the formation of the blood-brain barrier, as assessed by the penetrance of a fluorescent dye into the nerve cord.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
NOT suppressed by
Statement
Reference
NOT Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference
Co-expression of DhitKK109401, or a Dhitex183/Dhitex274 background, enhances the frequency of wing margin bristle defects in Scer\GAL4Bx-MS1096 GαoGTP.Scer\UAS flies.
Co-expression of Gnf1GD4455 with G-oα47AGTP.Scer\UAS in LN[[v]] neurons under the control of Scer\GAL4P0.5.Pdf does not affect the arrhythmicity or weak rhythms seen in G-oα47AGTP.Scer\UAS-expressing flies. Co-expression of dncScer\UAS.cCa with G-oα47AGTP.Scer\UAS in LN[[v]] neurons under the control of Scer\GAL4P0.5.Pdf results in low power short-period rhythms. Co-expression of Plc21CGD11359 with G-oα47AGTP.Scer\UAS in LN[[v]] neurons under the control of Scer\GAL4P0.5.Pdf results in strong rhythms. These flies exhibit longer period rhythms (24.7hr) than control flies.
The effects of overexpression of G-oα47AGTP.Scer\UAS are enhanced in fzunspecified homozygous wings. Co-overexpression of fzScer\UAS.cSa and G-oα47AGTP.Scer\UAS enhances the asymmetric division defects seen in G-oα47AGTP.Scer\UAS mutants.
The frequency of multiple wing hairs induced by expression of G-oα47AGTP.Scer\UAS under the control of Scer\GAL4Bx-MS1096 is not altered if the flies also carry fzunspecified.
Xenogenetic Interactions
Statement
Reference
Expression of GαoGTP.Scer\UAS does not suppress the rough eye phenotype seen when Hsap\APPAβ42.Scer\UAS is expressed pan-neuronally under the control of Scer\GAL4GMR.PU.
Co-expression of Mmus\Cd8aScer\UAS.T:Avic\GFP with G-oα47AGTP.Scer\UAS in LN[[v]] neurons under the control of Scer\GAL4P0.5.Pdf does not affect the arrhythmicity or weak rhythms seen in G-oα47AGTP.Scer\UAS-expressing flies.
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
G-oα47AGTP.Scer\UAS
GαoGTP.Scer\UAS
GαoGTP.UAS
Name Synonyms
Secondary FlyBase IDs
    References (9)