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General Information
Symbol
Dmel\GαqUAS.cRa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct of Ratnaparkhi
FlyBase ID
FBal0183499
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-Gαq
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of the wild-type Gαq 3 splice variant cDNA.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Gα49BScer\UAS.cRa; Scer\GAL4Ddc.PL flies a have slightly defective wing posture.

In embryos expressing Gα49BScer\UAS.cRa under the control of Scer\GAL4elav-C155, the pattern of the central nervous system fascicles and commissures appears normal, when visualised with BP102. Overexpression of Gα49BScer\UAS.cRa under the regulation of Scer\GAL4elav-C155 has no discernible effect on the embryonic stage central nervous system neuronal projection pattern, when visualised with Fas2.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Co-expression of Gr21aScer\UAS.cKa and Gr63aScer\UAS.cKa under the control of Scer\GAL4Or22a.8010 in the "empty neuron" system ("Δhalo" embryos - Dp(2;2)dppd21 + Df(2L)dp-79b - in which Or22a and Or22b are deleted) confers a modest response to CO[[2]] in the ab3A olfactory receptor neuron (ORN). Additional co-expression of Gα49BScer\UAS.cRa dramatically increases the response of the ab3A ORN to CO[[2]], to a level comparable to that of the endogenous CO[[2]] responsive neuron (ab1C).

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Co-expression of Gα49BScer\UAS.cRa restores a normal response of the ab1C olfactory receptor neuron to CO[[2]] in animals expressing either Gα49BdsRNA.Scer\UAS or Gα49BdsRNA.Scer\UAS.1f1 under the control of Scer\GAL4Gr21a.9.323.

Expression of Gα49BScer\UAS.cRa under the control of Scer\GAL4Or83b.2.642.T:Hsim\VP22 rescues the reduced electroantennogram amplitude in response to odours of animals containing large Gα49B221c or Gα49B1370 homozygous clones in the antenna.

Expression of Gα49BScer\UAS.cRa under the control of Scer\GAL4Or83b.2.642.T:Hsim\VP22 only during adulthood (expression is inhibited before this stage by expression of Scer\GAL80ts.αTub84B at 18[o]C, and the animals are shifted to 29[o]C to inactivate Scer\GAL80ts.αTub84B a few hours before eclosion) also rescues the reduced electroantennogram amplitude in response to odours of animals containing large Gα49B221c or Gα49B1370 homozygous clones in the antenna.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
Gα49BScer\UAS.cRa
GαqScer\UAS.cRa
GαqUAS.cRa
Name Synonyms
Saccharomyces cerevisiae UAS construct of Ratnaparkhi
Secondary FlyBase IDs
    References (8)