Allele Dmel\DopR^f02676

General Information
Symbol	Dmel\DopR^f02676	Species	D. melanogaster
Name		FlyBase ID	FBal0184074
Feature type	allele	Associated gene	Dmel\DopR

Map ( GBrowse )	Untitled Document
Allele class
Mutagen	piggyBac transposase
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	piggyBac transposase (Gene Disruption Project members and Exelixis, 2005)
Mutations Mapped to the Genome

Type Location Additional Notes References transposable element insertion site 3R:10,032,116..10,032,116 (Gene Disruption Project members and Exelixis, 2005, Kim et al., 2007, Inagaki et al., 2012, Kong et al., 2010, Liu et al., 2012)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference
Caused by insertion	PBac{WH}DopR^f02676 (Gene Disruption Project members and Exelixis, 2005, Kim et al., 2007, Inagaki et al., 2012, Kong et al., 2010, Liu et al., 2012)
Cytology
Phenotypic Data
Phenotypic Class
	hyperactive \| chemical conditional (Kong et al., 2010) hypoactive \| chemical conditional (with Df(3R)ED5634) (Kong et al., 2010) learning defective (with In(3LR)234) (Kim et al., 2007) learning defective \| recessive (Kim et al., 2007)
Phenotype Manifest In

Detailed Description
	Statement Reference DopR[f02676] mutant flies show a similar increase in sugar sensitivity in response to L-Dopa feeding to wild controls. (Inagaki et al., 2012) The decreased night-time sleep seen when wild-type flies are fed L-dopa is markedly suppressed in a DopR[f02676]/DopR[f02676] background. (Liu et al., 2012) DopR[f02676] mutants show reduced ethanol-induced hyperactivity and no change in ethanol sedation sensitivity or ethanol absorption. DopR[f02676] also show increased activity prior to ethanol exposure. DopR[f02676]/Df(3R)ED5634 flies show reduced ethanol-induced hyperactivity. DopR[f02676] mutants perform indistinguishably from wild-type in a rapid tolerance paradigm, showing both increased ethanol-induced hyperactivity and increased resistance to the sedating effects of ethanol during a second exposure to ethanol vapor. (Kong et al., 2010) DopR[f02676]/DopR[f02676] and DopR[f02676]/In(3LR)234 adults fail to learn to avoid specific odors after conditioning by concurrent shock and odor exposure. The same results are seen when testing is done immediately after conditioning or 1 hour after conditioning and with a variety of odors. These animals also have significantly reduced scores in tests for sugar reward mediated olfactory conditioning. (Kim et al., 2007)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement Reference DopR^f02676/DopR^f02676 is a suppressor of sleep defective \| heat sensitive phenotype of Scer\GAL4^ple.PF, TrpA1^Scer\UAS.cKa (Liu et al., 2012, Liu et al., 2012)
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference The reduction in nighttime sleep seen in flies expressing TrpA1[Scer\UAS.cKa] under the control of Scer\GAL4[ple.PF] at 27[o]C is fully suppressed by DopR[f02676]/DopR[f02676]. (Liu et al., 2012)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	DopR^f02676 is rescued by DopR^f02676/Scer\GAL4^Gr5a.8.5 (Inagaki et al., 2012, Inagaki et al., 2012) DopR^f02676 is rescued by DopR^Scer\UAS.cKa/Scer\GAL4^5.30 (Kong et al., 2010) DopR^f02676 is rescued by DopR^Scer\UAS.cKa/Scer\GAL4^11.148 (Kong et al., 2010) DopR^f02676 is rescued by DopR^Scer\UAS.cKa/Scer\GAL4^lilli-189Y (Kong et al., 2010) DopR^f02676 is rescued by Scer\GAL4^Mef2.247/Scer\GAL4^Mef2.247/DopR^f02676 (Kim et al., 2007, Kim et al., 2007, Kim et al., 2007) In(3LR)234/DopR^f02676 is rescued by Scer\GAL4^Mef2.247/Scer\GAL4^Mef2.247/DopR^f02676 (Kim et al., 2007, Kim et al., 2007)
Partially rescued by	DopR^f02676 is partially rescued by Scer\GAL4^104Y/DopR^f02676 (Liu et al., 2012, Liu et al., 2012) DopR^f02676 is partially rescued by Scer\GAL4^c205/DopR^f02676 (Liu et al., 2012, Liu et al., 2012) DopR^f02676 is partially rescued by Scer\GAL4^elav-C155/DopR^f02676 (Kim et al., 2007, Kim et al., 2007, Kim et al., 2007) In(3LR)234/DopR^f02676 is partially rescued by Scer\GAL4^elav-C155/DopR^f02676 (Kim et al., 2007, Kim et al., 2007)
Not rescued by	DopR^f02676 is not rescued by DopR^Scer\UAS.cKa/Scer\GAL4^17d (Kong et al., 2010) DopR^f02676 is not rescued by Scer\GAL4^Aph-4-c232/DopR^Scer\UAS.cKa (Kong et al., 2010) DopR^f02676 is not rescued by Scer\GAL4^c547/DopR^f02676 (Liu et al., 2012, Liu et al., 2012) DopR^f02676 is not rescued by Scer\GAL4^ey-OK107/DopR^f02676 (Liu et al., 2012, Liu et al., 2012) DopR^f02676 is not rescued by Scer\GAL4^Tab2-201Y/DopR^Scer\UAS.cKa (Kong et al., 2010)
Rescues	DopR^f02676/Scer\GAL4^Gr5a.8.5 rescues DopR^f02676 (Inagaki et al., 2012, Inagaki et al., 2012) Scer\GAL4^Mef2.247, Scer\GAL4^Mef2.247, DopR^f02676 rescues DopR^f02676 (Kim et al., 2007, Kim et al., 2007, Kim et al., 2007) Scer\GAL4^Mef2.247, Scer\GAL4^Mef2.247, DopR^f02676 rescues In(3LR)234/DopR^f02676 (Kim et al., 2007, Kim et al., 2007)
Partially rescues	Scer\GAL4^104Y/DopR^f02676 partially rescues DopR^f02676 (Liu et al., 2012, Liu et al., 2012) Scer\GAL4^c205/DopR^f02676 partially rescues DopR^f02676 (Liu et al., 2012, Liu et al., 2012) Scer\GAL4^elav-C155/DopR^f02676 partially rescues DopR^f02676 (Kim et al., 2007, Kim et al., 2007, Kim et al., 2007) Scer\GAL4^elav-C155/DopR^f02676 partially rescues In(3LR)234/DopR^f02676 (Kim et al., 2007, Kim et al., 2007)
Fails to rescue	Scer\GAL4^c547/DopR^f02676 fails to rescue DopR^f02676 (Liu et al., 2012, Liu et al., 2012) Scer\GAL4^ey-OK107/DopR^f02676 fails to rescue DopR^f02676 (Liu et al., 2012, Liu et al., 2012)
Comments	Expressing DopR[f02676] in Gr5a gustatory receptor neurons under the control of Scer\GAL4[Gr5a.8.5] rescues the defects in the sugar-sensitive proboscis extension reflex (PER) seen in DopR[f02676] mutant females. (Inagaki et al., 2012) Expression of DopR (from the Scer\UAS sequences in the PBac{WH}DopR[f02676] insertion) under the control of either Scer\GAL4[104Y] or Scer\GAL4[c205] significantly restores the the reduced sleep caused by dopamine signaling in DopR[f02676]/DopR[f02676] flies fed L-dopa. This effect is not seen if expression is driven either by Scer\GAL4[ey-OK107] or by Scer\GAL4[c547]. (Liu et al., 2012) Flies of the genotype Scer\GAL4[5.30]/+; DopR[f02676]/DopR[f02676] show selectively restored expression of DopR. Those flies that had restored expression in patterns that include the R2/R4 class ellipsoid body neurons exhibit rescue of ethanol-induced hyperactivity. Restoring DopR expression in the R3 neurons of the ellipsoid body neurons (through Scer\GAL4[lilli-189Y]) results in a modest but statistically significant increase in ethanol-induced hyperactivity. However, expression with Scer\GAL4[Aph-4-c232] has no effect on ethanol induced hyperactivity in DopR[f02676] homozygous mutants. (Kong et al., 2010) The failure of DopR[f02676]/In(3LR)234 or DopR[f02676]/DopR[f02676] adults to learn to avoid specific odors after conditioning by concurrent shock and odor exposure is partially rescued by Scer\GAL4[elav-C155] and completely rescued by Scer\GAL4[Mef2.247]. Scer\GAL4[elav-C155] and Scer\GAL4[Mef2.247] also completely rescued learning by sugar-reward conditioning in these flies. (In all cases, rescue was tested in the presence of Scer\GAL80[ts.αTub84B] and rescued was seen at 30'C but not at room temperature.) (Kim et al., 2007)
Stocks ( 1 )
Harvard	- PBac{WH}DopR^f02676
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	DopR^f02676 (Ueno et al., 2013, Liu et al., 2012) dumb² (Kim et al., 2007)
Name Synonym
Secondary FlyBase IDs

References ( 6 )
Research paper	Ueno et al., 2013, J. Physiol. 591(1): 287--302 Long-term enhancement of synaptic transmission between antennal lobe and mushroom body in cultured Drosophila brain. [FBrf0220358] Inagaki et al., 2012, Cell 148(3): 583--595 Visualizing Neuromodulation In Vivo: TANGO-Mapping of Dopamine Signaling Reveals Appetite Control of Sugar Sensing. [FBrf0217362] Liu et al., 2012, Curr. Biol. 22(22): 2114--2123 Two dopaminergic neurons signal to the dorsal fan-shaped body to promote wakefulness in Drosophila. [FBrf0220041] Kong et al., 2010, PLoS ONE 5(4): e9954 A pair of dopamine neurons target the D1-like dopamine receptor DopR in the central complex to promote ethanol-stimulated locomotion in Drosophila. [FBrf0210514] Kim et al., 2007, J. Neurosci. 27(29): 7640--7647 D1 dopamine receptor dDA1 is required in the mushroom body neurons for aversive and appetitive learning in Drosophila. [FBrf0202085]
Personal communication to FlyBase	Gene Disruption Project members and Exelixis, 2005, Genomic mapping of Exelixis insertion collection. (Computer file) Genomic mapping of Exelixis insertion collection. (Computer file) [FBrf0184340]

Allele Dmel\DopRf02676

Allele Dmel\DopR^f02676