FB2025_01 , released February 20, 2025
Allele: Dmel\Cdc42V12.UAS.Tag:MYC
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General Information
Symbol
Dmel\Cdc42V12.UAS.Tag:MYC
Species
D. melanogaster
Name
FlyBase ID
FBal0184361
Feature type
allele
Associated gene
Dmel\Cdc42
Associated Insertion(s)
Carried in Construct
P{UAS-cdc42.V12.myc}
Also Known As
UAS-myc::Dcdc42V12
Key Links
Transgenic product class
missense_variant
(Kim et al., 2002)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Kim et al., 2002)
Mutagen
in vitro construct
(Kim et al., 2003, Kim et al., 2002)
Progenitor genotype
Carried in construct
P{UAS-cdc42.V12.myc}
Cytology
Description

UASt regulatory sequences drive expression of a mutated form of Cdc42 which carries the V12 constitutive activation mutation. The protein is tagged with two copies of Tag:MYC.

(Kim et al., 2002)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Cdc42
Tagged with
Tag:MYC
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In

      abdominal posterior fascicle neuron & growth cone & filopodium, with Scer\GAL4elav.PLu

      (Kim et al., 2003)

      larval abdominal segmental nerve, with Scer\GAL4elav.PLu

      (Kim et al., 2003)

      mesothoracic les3 neuron & growth cone & filopodium, with Scer\GAL4elav.PLu

      (Kim et al., 2003)

      metathoracic les3 neuron & growth cone & filopodium, with Scer\GAL4elav.PLu

      (Kim et al., 2003)
      Detailed Description
      Statement
      Reference

      In Cdc42V12.Scer\UAS.T:Hsap\MYC; Scer\GAL4elav.PLu embryos, segmental nerve (SN)a motor axons (part of the abdominal posterior fascicle) misroute or stall before reaching their target muscles. SNb motoneuron axons (also part of the abdominal posterior fascicle) also stall prematurely, whereas intersegmental nerve (ISN) axons (anterior fascicle ) are normal. In Cdc42V12.Scer\UAS.T:Hsap\MYC; Scer\GAL4elav.PLu embryos, segmental nerve (SN)b motor growth cones have significantly longer filopodia, with higher rates of extension and retraction compared to wild-type. These animals also exhibit a significant increase in filopodial activity in LBD sensory neuron (innervating the Kolbchen) growth cones.

      (Kim et al., 2003)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT suppressed by
      Statement
      Reference

      Cdc42V12.UAS.Tag:MYC, Scer\GAL4elav.PLu has abnormal neuroanatomy phenotype, non-suppressible by Pak11/Pak6

      (Kim et al., 2003)
      Phenotype Manifest In
      NOT suppressed by
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The axon guidance defects seen in Cdc42V12.Scer\UAS.T:Hsap\MYC; Scer\GAL4elav.PLu embryos are unaffected by the background Pak6/Pak11.

      (Kim et al., 2003)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer

      Kim

      (Kim et al., 2003)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Cdc42V12.Scer\UAS.T:Hsap\MYC
      Cdc42V12.UAS.Tag:MYC
      n
      (Kim et al., 2003)
      Name Synonyms
      Secondary FlyBase IDs
        References (3)