Allele Dmel\ArfGAP3^e01250

General Information
Symbol	Dmel\ArfGAP3^e01250	Species	D. melanogaster
Name		FlyBase ID	FBal0185122
Feature type	allele	Associated gene	Dmel\ArfGAP3

Map ( GBrowse )	Untitled Document
Allele class
Mutagen	piggyBac transposase
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	piggyBac transposase (Gene Disruption Project members and Exelixis, 2005)
Mutations Mapped to the Genome

Type Location Additional Notes References transposable element insertion site 3L:22,710,551..22,710,551 (Gene Disruption Project members and Exelixis, 2005, Johnson et al., 2011)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference
Caused by insertion	PBac{RB}ArfGAP3^e01250 (Gene Disruption Project members and Exelixis, 2005, Johnson et al., 2011)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In
	ommatidium (Johnson et al., 2011) retina (Johnson et al., 2011)
Detailed Description
	Statement Reference Flies homozygous for ArfGAP3[e01250] that survive to 27 h after puparium formation (APF) display mild patterning defects in their retina. (Johnson et al., 2011)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhancer of
Statement Reference ArfGAP3^e01250/CG6838[+] is an enhancer of cone cell phenotype of Arf51F^GD13822, Dcr-2^Scer\UAS.cDa, Scer\GAL4^GMR.PF (Johnson et al., 2011) ArfGAP3^e01250/CG6838[+] is an enhancer of inter-ommatidial precursor cell phenotype of Arf51F^GD13822, Dcr-2^Scer\UAS.cDa, Scer\GAL4^GMR.PF (Johnson et al., 2011) ArfGAP3^e01250/CG6838[+] is an enhancer of ommatidium phenotype of Arf51F^GD13822, Dcr-2^Scer\UAS.cDa, Scer\GAL4^GMR.PF (Johnson et al., 2011) ArfGAP3^e01250/CG6838[+] is an enhancer of ommatidium phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, cindr^{dsRNA.PC.PD.Scer\UAS} (Johnson et al., 2011) ArfGAP3^e01250/CG6838[+] is an enhancer of primary pigment cell phenotype of Arf51F^GD13822, Dcr-2^Scer\UAS.cDa, Scer\GAL4^GMR.PF (Johnson et al., 2011) ArfGAP3^e01250/CG6838[+] is an enhancer of retina phenotype of Arf51F^GD13822, Dcr-2^Scer\UAS.cDa, Scer\GAL4^GMR.PF (Johnson et al., 2011) ArfGAP3^e01250/CG6838[+] is an enhancer of secondary pigment cell phenotype of Arf51F^GD13822, Dcr-2^Scer\UAS.cDa, Scer\GAL4^GMR.PF (Johnson et al., 2011) ArfGAP3^e01250/CG6838[+] is an enhancer of tertiary pigment cell phenotype of Arf51F^GD13822, Dcr-2^Scer\UAS.cDa, Scer\GAL4^GMR.PF (Johnson et al., 2011)
Additional Comments
Genetic Interactions
Statement Reference The eye mis-patterning phenotype resulting from the co-expression of Arf51F[GD13822] with Dcr-2[Scer\UAS.cDa] under the control of Scer\GAL4[GMR.PF] is significantly enhanced in ArfGAP3[e01250] heterozygotes. The number of correctly patterned secondary and tertiary cell is reduced. The total number of interommatidial precursor cells (IPCs) does not differ markedly from the wild-type number of 12, indicating that the enhancement is due to specifically to increased disorder in IPC patterning. Additionally, errors in the placement of three bristle groups about each ommatidium and errors in primary pigment cell and cone cell patterning and ommatidial rotation are modestly enhanced. The ommatidial patterning errors resulting from the expression of cindr[dsRNA.PC.PD.Scer\UAS] via Scer\GAL4[GMR.PF] are strongly increased in ArfGAP3[e01250] heterozygotes. (Johnson et al., 2011)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 1 )
Harvard	- PBac{RB}ArfGAP3^e01250
Notes on Origin
Discoverer
	Separable from: PBac{RB}e01250. (Gene Disruption Project members and Exelixis, 2005)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	ArfGAP3^e01250 CG6838^e01250
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Johnson et al., 2011, Mol. Biol. Cell 22(23): 4513--4526 Role for a Cindr-Arf6 axis in patterning emerging epithelia. [FBrf0216708]
Personal communication to FlyBase	Gene Disruption Project members and Exelixis, 2005, Genomic mapping of Exelixis insertion collection. (Computer file) Genomic mapping of Exelixis insertion collection. (Computer file) [FBrf0184340]

Allele Dmel\ArfGAP3e01250

Allele Dmel\ArfGAP3^e01250