FlyBase Allele Report: Dmel\Lrrk[e03680]

(Inoshita et al., 2022, Fellgett et al., 2021, Afsari et al., 2014, Kanao et al., 2012, Lee et al., 2010, Lin et al., 2010, Gene Disruption Project members and Exelixis, 2005)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Parkinson's disease

CEA

(Lin et al., 2015)

model of Parkinson's disease 8

CEA

(Fellgett et al., 2021, Sarkar et al., 2021, Lee et al., 2007)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Parkinson's disease

modeled by Pink1^B9

(Tain et al., 2009)

modeled by park²⁵

(Tain et al., 2009)

exacerbates Parkinson's disease 1

modeled by Hsap\SNCA^QUAS.cOa

(Sarkar et al., 2021)

model of Parkinson's disease 8

is ameliorated by tsr^{UAS.Tag:polyHis}

(Sarkar et al., 2021)

Comments on Models/Modifiers Based on Experimental Evidence ( 1 )

Dendritic phenotypes are observed when Lrrk^e03680 is in combination with Df(3R)del6.

(Lin et al., 2015)

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior

(Lee et al., 2007)

abnormal neuroanatomy

(Lee et al., 2010)

abnormal neuroanatomy (with Df(3R)del6)

(Lin et al., 2015)

abnormal neuroanatomy (with Df^Lrrk)

(Lee et al., 2010)

abnormal neuroanatomy | dominant | third instar larval stage

(Inoshita et al., 2017)

abnormal neuroanatomy | recessive | third instar larval stage

(Penney et al., 2016)

abnormal neuroanatomy | third instar larval stage (with Lrrk^ex1)

(Penney et al., 2016)

abnormal neurophysiology

(Lee et al., 2010)

abnormal neurophysiology | adult stage | conditional

(Soukup et al., 2016)

abnormal neurophysiology | third instar larval stage

(Matta et al., 2012)

decreased body size

(Chuang et al., 2014)

decreased cell number | adult stage | conditional

(Soukup et al., 2016)

decreased fecundity | female | progressive

(Lee et al., 2007)

female semi-sterile

(Dodson et al., 2012)

female semi-sterile (with Df(3R)BSC141)

(Dodson et al., 2012)

female sterile

(Lee et al., 2007)

viable

(Dodson et al., 2012)

viable (with Df(3R)BSC141)

(Dodson et al., 2012)

visible

(Chuang et al., 2014)

Phenotype Manifest In

autophagosome | increased number

(Sarkar et al., 2021)

autophagosome | third instar larval stage | nutrition conditional

(Soukup et al., 2016)

bouton | third instar larval stage

(Penney et al., 2016)

bouton | third instar larval stage (with Lrrk^ex1)

(Penney et al., 2016)

dendrite (with Df(3R)del6)

(Lin et al., 2015)

dendritic arborizing neuron (with Df(3R)del6)

(Lin et al., 2015)

dopaminergic neuron

(Lee et al., 2007)

embryonic/larval neuromuscular junction | third instar larval stage

(Penney et al., 2016)

embryonic/larval neuromuscular junction | third instar larval stage (with Lrrk^ex1)

(Penney et al., 2016)

eye

(Chuang et al., 2014)

Golgi stack (with Df(3R)del6)

(Lin et al., 2015)

lamina | adult stage | conditional

(Soukup et al., 2016)

late endosome

(Dodson et al., 2014)

mitochondrion

(Lee et al., 2010)

neuromuscular junction

(Lee et al., 2010)

neuromuscular junction (with Df^Lrrk)

(Lee et al., 2010)

NMJ bouton

(Lee et al., 2010)

NMJ bouton (with Df^Lrrk)

(Lee et al., 2010)

ommatidium

(Chuang et al., 2014)

ommatidium | adult stage | conditional

(Soukup et al., 2016)

outer medulla | adult stage

(Sarkar et al., 2021)

photoreceptor neuron | adult stage | conditional

(Soukup et al., 2016)

rhabdomere | adult stage | conditional

(Soukup et al., 2016)

type I terminal bouton | third instar larval stage

(Inoshita et al., 2017)

Detailed Description

Statement

Reference

Lrrk^e03680 homozygotes show an increased number of autophagosomes (Atg8a-positive puncta) in the anterior medulla.

(Sarkar et al., 2021)

Lrrk^e03680 heterozygotes exhibit a significant increase in the number of type Ib synaptic boutons at the third instar larval neuromuscular junction, as compared to wild-type controls.

(Inoshita et al., 2017)

Lrrk^e03680/+, Lrrk^e03680/Lrrk^e03680 or Lrrk^e03680/Lrrk^ex1 mutant larval neuromuscular junctions do not exhibit any significant difference in amplitude of miniature excitatory junction currents (mEJCs), EJCs, or quantal content, as compared to controls. Lrrk^e03680/Lrrk^e03680 and Lrrk^e03680/Lrrk^ex1, but not Lrrk^e03680/+, mutant larval neuromuscular junctions exhibit an increase in the total number of synaptic boutons, but Lrrk^e03680/Lrrk^e03680 mutants do not show a difference in the total number of presynaptic release sites per NMJ.

(Penney et al., 2016)

In Lrrk^e03680 homozygotes, the presynaptic region of third instar larval neuromuscular junctions fails to present the expected starvation-induced formation of autophagosomes (detected as fluorescent puncta of Atg8 or Lamp in confocal microscopy images), but presents no defects in autophagosome density under normal diet, as compared to controls; In adults reared under constant light, but not under constant dark, the eye exhibits a significant decrease in depolarization amplitude in response to light pulses (at day 3 of light exposure) and significant decreases in the rhabdomere number per ommatidium and in the photoreceptor terminal number per lamina cartridge (at day 7 of light exposure), as compared to controls;

(Soukup et al., 2016)

Df(3R)del6/Lrrk^e03680 mutant dendritic arborizing (da) neurons contain fewer stationary golgi outposts (GOPs) than in controls, and fewer than in Df(3R)del6 heterozygotes alone. Most of the dynamic outposts move preferentially in the anterograde direction (towards dendritic ends) rather than the retrograde shift (towards cell bodies) seen in Df(3R)del6/+ alone. Displacement in the anterograde direction is significantly increased compared to controls, but in the retrograde direction is slightly but not significantly decreased. Velocity of movement is increased in both directions compared with controls. The distribution of GOPs is shifted distally compared with Df(3R)del6/+ but the number of GOPs is not significantly different.

Terminal dendrites of Df(3R)del6/Lrrk^e03680 mutant dendritic arborising (da) neurons show no significant difference in the displacement of extension or retraction, but are more frequent in extension and less frequent in retraction than Df(3R)del6/+ controls. Dendritic arborization is more complex than in controls, with increased terminal branches. This increase in dendrites is seen in class IV neurons, but not in class I.

(Lin et al., 2015)

The overall organization and structure of homozygous Lrrk^e03680 mutant flies appears normal except reduced body size, including compound eyes. There are fewer ommatidia in Lrrk^e03680 mutant flies compared to controls.

(Chuang et al., 2014)

Lrrk^e03680 homozygous flies do not show premature follicle cell death.

Lrrk^e03680 homozygous flies show a mild increase in the size of the late endosomal compartment in a subset of cells, but no difference in lysosome morphology.

(Dodson et al., 2014)

Homozygous and Lrrk^e03680/Df(3R)BSC141 females show a dramatic reduction in fertility compared to wild type.

(Dodson et al., 2012)

The amplitude of the excitatory junctional potential (EJP) at the neuromuscular junction of mutant third instar larvae does not show a difference compared to controls at low-frequency stimulation in 2mM external calcium. However the mutants fail to maintain release during intense (10Hz) stimulation in 2mM calcium. mEJP amplitude in 0.5mM Ca[2+] is markedly increased compared to controls.

Ultrastructurally, mutant boutons stimulated using 60mM Kcl for 1 minute prior to fixation show an increased density of cisternal structures and larger vesicles at the expense of normal-sized synaptic vesicles compared to wild-type stimulated boutons.

(Matta et al., 2012)

Transheterozygous Lrrk^e03680/Df^Lrrk mutants display neuromuscular junction overgrowth and irregularly shaped boutons. These mutants exhibit a 41% increase in type I boutons compared to controls. Type Ib bouton number is not significantly changed, whereas type Is or irregularly shaped small-sized boutons are significantly increased. No significant change in muscle size is observed in these mutants. Average bouton size in Lrrk^e03680/Df^Lrrk mutants is noticeably smaller. Neuromuscular junction length and branch number in Lrrk^e03680/Df^Lrrk mutants after normalisation with muscle size are increased by 56 and 38%, respectively. Lrrk^e03680 homozygous mutants show similar phenotypes, although the bouton number increase is slightly less than that seen in Lrrk^e03680/Df^Lrrk mutants.

Lrrk^e03680 mutants exhibit a significant increase of futsch-positive MT loops, suggesting that Lrrk controls the organisation of MT cytoskeleton.

Newly synthesized tubulin inside synaptic boutons appears more elaborate and tangled in Lrrk^e03680 mutants.

Lrrk^e03680 mutants exhibit an increase in mitochondria number in terminal boutons.

Lrrk^e03680 mutants do not display any significant difference in mEJC amplitude compared with wild-type controls. mEJC frequency is significantly increased in Lrrk^e03680 mutants. Lrrk^e03680 heterozygous animals show a moderate increase of mEJC frequency. In terms of EJC amplitude, Lrrk^e03680 mutants show a significant reduction. Quantal content, a representation of synaptic junction efficacy, is also significantly reduced in Lrrk^e03680 mutants.

(Lee et al., 2010)

Dendrite arborization appears normal in dendritic arborization (DA) neurons of homozygous Lrrk^e03680 larvae.

(Lin et al., 2010)

Lrrk^e03680 females show a marked reduction in fertility and fecundity, both of which become severer with age. Homozygous males are fully fertile. Animals show severely impaired locomotor activity.

Dopaminergic neurons in the dorsomedial region of Lrrk^e03680 mutant brains display a shrunken morphology.

(Lee et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Lrrk^e03680 has abnormal neuroanatomy | dominant | third instar larval stage phenotype, suppressible by Vps35^MH20/Vps35[+]

(Inoshita et al., 2017)

Df(3R)del6/Lrrk^e03680 has abnormal neuroanatomy phenotype, suppressible by Hsap\LRRK2^{UAS.cLa.Tag:FLAG}/Scer\GAL4^ppk.PU

(Lin et al., 2015)

Lrrk^e03680 has visible phenotype, suppressible by Hsap\LRRK2^UAS.cCa/Scer\GAL4^GMR.PU

(Chuang et al., 2014)

Lrrk^e03680 has abnormal neurophysiology | third instar larval stage phenotype, suppressible by endoA[+]/EndoA^Δ4

(Matta et al., 2012)

Lrrk^e03680 has abnormal neuroanatomy phenotype, suppressible by Thor^k13517

(Lee et al., 2010)

Lrrk^e03680 has abnormal neuroanatomy phenotype, suppressible by futsch^N94/futsch[+]

(Lee et al., 2010)

Enhancer of

Statement

Reference

Lrrk^e03680/Lrrk^e03680 is an enhancer of abnormal locomotor behavior | adult stage phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Sarkar et al., 2021)

Lrrk^e03680/Lrrk^e03680 is an enhancer of decreased cell number | adult stage phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Sarkar et al., 2021)

Lrrk[+]/Lrrk^e03680 is an enhancer of visible | adult stage phenotype of Delta³

(Imai et al., 2015)

Suppressor of

Statement

Reference

Lrrk[+]/Lrrk^e03680 is a suppressor of abnormal size | third instar larval stage phenotype of Vps35^MH20/Vps35¹

(Inoshita et al., 2017)

Lrrk[+]/Lrrk^e03680 is a suppressor of abnormal circadian behavior | dominant | adult stage phenotype of Vps35¹

(Inoshita et al., 2017)

Lrrk[+]/Lrrk^e03680 is a suppressor of abnormal neuroanatomy | dominant | third instar larval stage phenotype of Vps35^MH20

(Inoshita et al., 2017)

Lrrk[+]/Lrrk^e03680 is a suppressor of abnormal neurophysiology | third instar larval stage phenotype of Scer\GAL4^Mhc.PW, mTor^{WT.UAS.Tag:FLAG}

(Penney et al., 2016)

Lrrk[+]/Lrrk^e03680 is a suppressor | partially of abnormal neuroanatomy phenotype of Scer\GAL4^109(2)80, lva^DN.UAS

(Lin et al., 2015)

Lrrk[+]/Lrrk^e03680 is a suppressor of abnormal neurophysiology | progressive phenotype of Hsap\LRRK2^{G2019S.UAS.Tag:FLAG}, Scer\GAL4^ple.PF

(Hindle et al., 2013)

Lrrk[+]/Lrrk^e03680 is a suppressor | partially of abnormal neuroanatomy | third instar larval stage phenotype of Avic\GFP^{UAS.S65T.I167T.Tag:MT(btau)}, Scer\GAL4^109(2)80

(Lin et al., 2010)

Lrrk[+]/Lrrk^e03680 is a suppressor | partially of abnormal neuroanatomy | third instar larval stage phenotype of Avic\GFP^{UAS.S65T.I167T.Tag:MT(btau)}, Scer\GAL4^ppk.PG

(Lin et al., 2010)

Lrrk^e03680 is a suppressor of abnormal neuroanatomy | adult stage phenotype of Pink1^B9

(Tain et al., 2009)

Lrrk^e03680 is a suppressor | partially of abnormal flight | recessive phenotype of Pink1^B9

(Tain et al., 2009)

Lrrk^e03680 is a suppressor | partially of abnormal locomotor behavior | recessive | adult stage phenotype of Pink1^B9

(Tain et al., 2009)

Lrrk^e03680 is a suppressor of abnormal neuroanatomy | adult stage phenotype of park²⁵

(Tain et al., 2009)

Lrrk^e03680 is a suppressor of abnormal flight | recessive phenotype of park²⁵

(Tain et al., 2009)

Lrrk^e03680 is a suppressor of abnormal locomotor behavior | recessive | adult stage phenotype of park²⁵

(Tain et al., 2009)

NOT Suppressor of

Statement

Reference

Lrrk^e03680/Lrrk^e03680 is a non-suppressor of lethal - all die before end of prepupal stage phenotype of Vps35^MH20/Vps35¹

(Inoshita et al., 2017)

Lrrk[+]/Lrrk^e03680 is a non-suppressor of visible | adult stage phenotype of N^55e11

(Imai et al., 2015)

Lrrk^e03680/Lrrk^e03680 is a non-suppressor of visible phenotype of Scer\GAL4^GMR.PF, for^P1.UAS, foxo^UAS.cPa

(Kanao et al., 2012)

Other

Statement

Reference

GluRIIA^SP16/Df(2L)cl-h4, Lrrk^e03680 has abnormal neurophysiology | third instar larval stage phenotype

(Penney et al., 2016)

GluRIIA^{M614R.UAS.Tag:MYC}, Lrrk[+]/Lrrk^e03680, Scer\GAL4^Mhc.PW has abnormal neurophysiology | third instar larval stage phenotype

(Penney et al., 2016)

Phenotype Manifest In

Suppressed by

Statement

Reference

Lrrk^e03680 has outer medulla | adult stage phenotype, suppressible | partially by Scer\GAL4^nSyb.PP/tsr^{UAS.Tag:polyHis}

(Sarkar et al., 2021)

Lrrk^e03680 has autophagosome | increased number phenotype, suppressible | partially by Scer\GAL4^nSyb.PP/tsr^{UAS.Tag:polyHis}

(Sarkar et al., 2021)

Lrrk^e03680 has type I bouton | third instar larval stage phenotype, suppressible by Vps35^MH20/Vps35[+]

(Inoshita et al., 2017)

Lrrk^e03680 has autophagosome | third instar larval stage | nutrition conditional phenotype, suppressible by Scer\GAL4^Toll-6-D42/Hsap\LRRK2^UAS.Tag:V5

(Soukup et al., 2016)

Lrrk^e03680 has autophagosome | third instar larval stage | nutrition conditional phenotype, suppressible by EndoA^S75D.CyPet

(Soukup et al., 2016)

Df(3R)del6/Lrrk^e03680 has dendritic arborizing neuron phenotype, suppressible by Hsap\LRRK2^{UAS.cLa.Tag:FLAG}/Scer\GAL4^ppk.PU

(Lin et al., 2015)

Df(3R)del6/Lrrk^e03680 has dendrite phenotype, suppressible by Hsap\LRRK2^{UAS.cLa.Tag:FLAG}/Scer\GAL4^ppk.PU

(Lin et al., 2015)

Df(3R)del6/Lrrk^e03680 has Golgi stack phenotype, suppressible by Hsap\LRRK2^{UAS.cLa.Tag:FLAG}/Scer\GAL4^ppk.PU

(Lin et al., 2015)

Lrrk[+]/Lrrk^e03680, Scer\GAL4^109(2)80, lva^DN.UAS has dendritic arborizing neuron phenotype, suppressible | partially by Df(3R)del6/+

(Lin et al., 2015)

Lrrk^e03680 has eye phenotype, suppressible by Hsap\LRRK2^UAS.cCa/Scer\GAL4^GMR.PU

(Chuang et al., 2014)

Lrrk^e03680 has neuromuscular junction phenotype, suppressible by Thor^k13517

(Lee et al., 2010)

Lrrk^e03680 has NMJ bouton phenotype, suppressible by Thor^k13517

(Lee et al., 2010)

Lrrk^e03680 has neuromuscular junction phenotype, suppressible by futsch^N94/futsch[+]

(Lee et al., 2010)

Lrrk^e03680 has NMJ bouton phenotype, suppressible by futsch^N94/futsch[+]

(Lee et al., 2010)

NOT suppressed by

Statement

Reference

Lrrk^e03680 has autophagosome | third instar larval stage | nutrition conditional phenotype, non-suppressible by EndoA^S75A.CyPet

(Soukup et al., 2016)

Enhancer of

Statement

Reference

Lrrk^e03680/Lrrk^e03680 is an enhancer of outer medulla | adult stage phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Sarkar et al., 2021)

Lrrk^e03680/Lrrk^e03680 is an enhancer of medullary intrinsic neuron | adult stage | decreased number phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Sarkar et al., 2021)

Lrrk^e03680/Lrrk^e03680 is an enhancer of dopaminergic medulla neuron | adult stage | decreased number phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Sarkar et al., 2021)

Lrrk[+]/Lrrk^e03680 is an enhancer of wing vein | adult stage phenotype of Delta³

(Imai et al., 2015)

Lrrk^e03680 is an enhancer of lysosome phenotype of Rab7^{Q67L.UASp.YFP}, Scer\GAL4^CY2

(Dodson et al., 2012)

Suppressor of

Statement

Reference

Lrrk[+]/Lrrk^e03680 is a suppressor of type I terminal bouton | third instar larval stage phenotype of Vps35^MH20/Vps35¹

(Inoshita et al., 2017)

Lrrk[+]/Lrrk^e03680 is a suppressor of synaptic vesicle | third instar larval stage phenotype of Vps35^MH20/Vps35¹

(Inoshita et al., 2017)

Lrrk[+]/Lrrk^e03680 is a suppressor of type I bouton | third instar larval stage phenotype of Vps35^MH20

(Inoshita et al., 2017)

Lrrk[+]/Lrrk^e03680 is a suppressor of embryonic/larval neuromuscular junction | third instar larval stage phenotype of Scer\GAL4^Mhc.PW, mTor^{WT.UAS.Tag:FLAG}

(Penney et al., 2016)

Lrrk^e03680/Lrrk^e03680 is a suppressor | partially of wing margin phenotype of neur^{ΔRING.UAS.EGFP}

(Imai et al., 2015)

Lrrk[+]/Lrrk^e03680 is a suppressor | partially of dendritic arborizing neuron phenotype of Scer\GAL4^109(2)80, lva^DN.UAS

(Lin et al., 2015)

Lrrk[+]/Lrrk^e03680 is a suppressor | partially of dendritic arborizing neuron | third instar larval stage phenotype of Avic\GFP^{UAS.S65T.I167T.Tag:MT(btau)}, Scer\GAL4^109(2)80

(Lin et al., 2010)

Lrrk[+]/Lrrk^e03680 is a suppressor | partially of dendrite | third instar larval stage phenotype of Avic\GFP^{UAS.S65T.I167T.Tag:MT(btau)}, Scer\GAL4^109(2)80

(Lin et al., 2010)

Lrrk[+]/Lrrk^e03680 is a suppressor | partially of microtubule phenotype of Avic\GFP^{UAS.S65T.I167T.Tag:MT(btau)}, Scer\GAL4^ppk.PG

(Lin et al., 2010)

Lrrk[+]/Lrrk^e03680 is a suppressor | partially of larval multidendritic class IV neuron | third instar larval stage phenotype of Avic\GFP^{UAS.S65T.I167T.Tag:MT(btau)}, Scer\GAL4^ppk.PG

(Lin et al., 2010)

Lrrk^e03680 is a suppressor of dopaminergic PPL1 neuron phenotype of Pink1^B9

(Tain et al., 2009)

Lrrk^e03680 is a suppressor of dopaminergic PPL1 neuron phenotype of park²⁵

(Tain et al., 2009)

NOT Suppressor of

Statement

Reference

Lrrk[+]/Lrrk^e03680 is a non-suppressor of wing vein | adult stage phenotype of N^55e11

(Imai et al., 2015)

Lrrk^e03680/Lrrk^e03680 is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PF, for^P1.UAS, foxo^UAS.cPa

(Kanao et al., 2012)

Other

Statement

Reference

GluRIIA^SP16/Df(2L)cl-h4, Lrrk^e03680 has embryonic/larval neuromuscular junction | third instar larval stage phenotype

(Penney et al., 2016)

GluRIIA^{M614R.UAS.Tag:MYC}, Lrrk[+]/Lrrk^e03680, Scer\GAL4^Mhc.PW has embryonic/larval neuromuscular junction | third instar larval stage phenotype

(Penney et al., 2016)

EndoA^S75D.CyPet, Lrrk^e03680 has autophagosome | third instar larval stage | nutrition conditional phenotype

(Soukup et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Lrrk^e03680 homozygosity rescues the increased size and decreased number of synaptic vesicles in the third instar larval neuromuscular junction of Vps35¹/Vps35^MH20 transheterozygotes does not rescue their prepupal lethality.

Lrrk^e03680 heterozygosity suppresses the decreased sleep bouts observed in Vps35¹ heterozygous adults, even leading to their significant increase as compared to controls.

Double heterozygosity for Lrrk^e03680 and Vps35^MH20 suppresses the increased number of type Ib synaptic boutons observed in the third instar larval neuromuscular junction of Vps35^MH20 or Lrrk^e03680 heterozygotes.

(Inoshita et al., 2017)

Lrrk^e03680/+ blocks the increased quantal content seen in larval NMJs expressing GluRIIA^{M614R.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Mhc.PW, via a decrease in excitatory junction current (EJC) amplitude.

Lrrk^e03680/Lrrk^e03680 blocks the increased quantal content seen in GluRIIA^SP16/Df(2L)cl-h4 mutant larval NMJs, via a decrease in excitatory junction current (EJC) amplitude.

Lrrk^e03680/+ suppresses the increase in excitatory junction current amplitude and quantal content seen in larvae NMJs expressing Tor^{WT.Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^Mhc.PW.

(Penney et al., 2016)

The failure in starvation-induced autophagosome formation observed at the presynaptic region of Lrrk^e03680-homozygous third instar larval neuromuscular junctions is rescued by one copy of EndoA^{S75D.T:Avic\GFP-CyPet}, but not by one copy of EndoA^{S75A.T:Avic\GFP-CyPet}; under normal diet conditions, however, the presence of one copy of EndoA^{S75D.T:Avic\GFP-CyPet} leads to a significant increase in the density of autophagosomes at the same site.

(Soukup et al., 2016)

The vein thickening and ectopic veins phenotype characteristic for N^55e11 mutant adults is not modified by combination with a single copy of Lrrk^e03680.

The frequency of the vein thickening phenotype observed in Dl³ heterozygote adults is significantly increased by combination with a single copy of Lrrk^e03680.

The wing notching phenotype characteristic for adults expressing neur^{ΔRING.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^dpp.PU is partially suppressed by combination with Lrrk^e03680 in homozygous state.

(Imai et al., 2015)

One copy of Lrrk^e03680 suppresses the reduction in dendritic ends seen when lva^DN.Scer\UAS is expressed in dendritic arborizing neurons under the control of Scer\GAL4^109(2)80. Adding one copy of Df(3R)del6 further suppresses the phenotype.

(Lin et al., 2015)

The increased clustering of lysosomes which is seen in the follicle cells of females expressing Rab7^{Q67L.Scer\UAS.P\T.T:Avic\GFP-YFP} under the control of Scer\GAL4^CY2 is enhanced in a Lrrk^e03680 background.

(Dodson et al., 2012)

Co-expression of foxo^Scer\UAS.cPa and for^P1.Scer\UAS under the control of Scer\GAL4^GMR.PF results in degeneration of the eye. The severity of this phenotype is not suppressed byLrrk^e03680/Lrrk^e03680.

(Kanao et al., 2012)

endoA^Δ4/+ suppresses the reduced excitatory junctional potential amplitude which is seen at the neuromuscular junction of Lrrk^e03680 third instar larvae during 10Z stimulation in 2mM calcium.

(Matta et al., 2012)

Expression of foxo^Scer\UAS.cKb under the control of Scer\GAL4^{elav.Switch.PO} in a Lrrk^e03680/Lrrk^e03680 background in the presence of RU486 throughout the lifespan has no effect on lifespan.

(Kanao et al., 2010)

The synaptic overgrowth phenotype seen in Lrrk^e03680 homozygotes is suppressed by the simultaneous loss of Thor in a homozygous Thor^k13517 background.

Removal of futsch activity in a heterozygous futsch^N94 background rescues the synaptic overgrowth phenotype found in Lrrk^e03680 heterozygotes.

The significant increase of futsch-positive MT loops seen in Lrrk^e03680 mutants is unaffected by a Thor^k13517 mutant background.

(Lee et al., 2010)

Lrrk^e03680 suppresses flight and climbing defects as well as dopaminergic PPL1 neuron loss in park²⁵/park²⁵ or Pink1^B9/Pink1^B9 flies.

(Tain et al., 2009)

Xenogenetic Interactions

Statement

Reference

The failure of Lrrk^e03680 homozygotes to exhibit starvation-induced formation of autophagosomes at the third instar larval neuromuscular junction presynaptic region is suppressed by the expression of Hsap\LRRK2^{Scer\UAS.T:SV5\V5} under the control of Scer\GAL4^Toll-6-D42.

(Soukup et al., 2016)

Expression of Hsap\LRRK2^{Scer\UAS.cLa.T:Zzzz\FLAG} in dendritic arborizing neurons under the control of either Scer\GAL4^109(2)80 or Scer\GAL4^ppk.PU suppresses the dendrite overgrowth seen in Df(3R)del6/Lrrk^e03680 mutants.

Expression of Hsap\LRRK2^{Scer\UAS.cLa.T:Zzzz\FLAG} in dendritic arborizing class IV neurons under the control of Scer\GAL4^ppk.PU suppresses the preference for anterograde movement (towards dendritic ends) seen in Df(3R)del6/Lrrk^e03680 mutant golgi outposts (GOPs), with the resulting GOPs showing the retrograde preference (towards cell bodies) seen in the Scer\GAL4^ppk.PU control.

(Lin et al., 2015)

Expression of Hsap\LRRK2^Scer\UAS.cCa under the control of Scer\GAL4^GMR.PU rescues the reduced eye size seen in Lrrk^e03680 mutant flies.

(Chuang et al., 2014)

Lrrk^e03680/+ partially suppresses the reduction in electroretinogram amplitude seen in 28 day old flies expressing Hsap\LRRK2^{G2019S.Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^ple.PG.

(Hindle et al., 2013)

Lrrk^e03680/+ partially suppresses the dendrite degeneration phenotype resulting from the expression of Scer\GAL4^109(2)80>Avic\GFP^{Scer\UAS.S65T.I167T.T:Btau\MAPT}. Dendrites of the Avic\GFP^{Scer\UAS.S65T.I167T.T:Btau\MAPT}-expressing neurons are longer and the number of dendritic ends is increased. The suppression by Lrrk^e03680/+ is also observed in inclusion formation and microtubule fragmentation.

(Lin et al., 2010)

Complementation and Rescue Data

Rescued by

Lrrk^e03680 is rescued by Lrrk^{+tCH322-120O10}

(Soukup et al., 2016)

Df(3R)del6/Lrrk^e03680 is rescued by Scer\GAL4^ppk.PU/Lrrk^UAS.Venus

(Lin et al., 2015)

Df(3R)BSC141/Lrrk^e03680 is rescued by Scer\GAL4^CY2/Lrrk^UAS.cDa

(Dodson et al., 2012)

Df(3R)BSC141/Lrrk^e03680 is rescued by Scer\GAL4^CY2/Lrrk^G1914S.UAS

(Dodson et al., 2012)

Lrrk^e03680 is rescued by Scer\GAL4^Mhc.PW/Lrrk^UAS.cIa

(Lee et al., 2010)

Lrrk^e03680 is rescued by Scer\GAL4^elav-C155/Lrrk^UAS.cIa

(Lee et al., 2010)

Lrrk^e03680 is rescued by Lrrk^{WT.UAS.Tag:FLAG}/Scer\GAL4^hs.PB

(Lee et al., 2007)

Partially rescued by

Lrrk^e03680 is partially rescued by Lrrk^Tag:MYC

(Dodson et al., 2012)

Not rescued by

Lrrk^e03680 is not rescued by Scer\GAL4^Mhc.PW/Lrrk^3KD.UAS

(Lee et al., 2010)

Lrrk^e03680 is not rescued by Scer\GAL4^elav-C155/Lrrk^3KD.UAS

(Lee et al., 2010)

Comments

The failure of Lrrk^e03680 homozygotes to exhibit starvation-induced formation of autophagosomes at the third instar larval neuromuscular junction presynaptic region is rescued by one copy of Lrrk^{+tCH322-120O10}.

(Soukup et al., 2016)

Expression of Lrrk^{Scer\UAS.T:Avic\GFP-YFP.Venus} in class IV dendritic arborizing neurons under the control of Scer\GAL4^ppk.PU rescues the reduction in golgi outposts in the dendrites of Lrrk^e03680/Df(3R)del6 mutants. The preference for anterograde movement (towards dendritic ends) is reverted to the preference for retrograde movement (towards cell bodies) seen in Df(3R)del6/+ and the increased anterograde displacement is suppressed. The mean velocities are returned to close to control levels. The increase in dendritic arborization seen in Lrrk^e03680/Df(3R)del6 mutants is suppressed when Lrrk^{Scer\UAS.T:Avic\GFP-YFP.Venus} is expressed in all da neurons under the control of Scer\GAL4^109(2)80.

(Lin et al., 2015)

A single copy of Lrrk^T:Hsap\MYC partially rescues the reduced fertility of Lrrk^e03680 homozygous females.

Expression of Lrrk^Scer\UAS.cDa under the control of Scer\GAL4^CY2 fully rescues the reduced fertility of Lrrk^e03680/Df(3R)BSC141 females.

(Dodson et al., 2012)

Muscle expression of Lrrk^Scer\UAS.cIa (under the control of Scer\GAL4^Mhc.PW) rescues the synaptic morphology phenotype and average bouton size found in Lrrk^e03680 homozygous mutants.

Neuronal expression of Lrrk^Scer\UAS.cIa (under the control of Scer\GAL4^elav-C155 results in a partial but still significant rescue of synaptic morphology and average bouton size in Lrrk^e03680 mutants.

Muscle or neuronal expression of Lrrk^3KD.Scer\UAS (under the control of Scer\GAL4^Mhc.PW or Scer\GAL4^elav-C155 respectively) fails to rescue the synaptic morphology phenotype and average bouton size found in Lrrk^e03680 homozygous mutants.

Muscle expression of Lrrk^Scer\UAS.cIa (through Scer\GAL4^Mhc.PW) fails to rescue the alterations of mEJC frequency, EJC amplitude, or quantal content in Lrrk^e03680 mutants.

Neuronal expression of Lrrk^Scer\UAS.cIa (through Scer\GAL4^elav-C155) partially rescues EJC amplitude in Lrrk^e03680 mutants, but fails to significantly rescue quantal content.

(Lee et al., 2010)

The climbing defects of Lrrk^e03680 mutant animals is rescued by expression of Lrrk^{WT.Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^hs.PB.

(Lee et al., 2007)

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Mutant

Wild-type

Stocks (1)

Bloomington

85160

w¹¹¹⁸; PBac{RB}Lrrk^e03680

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (14)

Reported As

Symbol Synonym

CG5483^e03680

LRRK^P1

(Lee et al., 2007)

LRRK^e03680

(Tain et al., 2009)

Lrrk^P1

(Soukup et al., 2016, Matta et al., 2012)

Lrrk^P1e03680

(Soukup et al., 2016)

Lrrk^e03680

(Inoshita et al., 2022, Sarkar et al., 2021)

LrrkP1

(Hernandez-Diaz et al., 2022)

dLRRK e03680

(Fellgett et al., 2021)

dLRRK(e03680)

(Hindle et al., 2013)

dLRRK^-

(Imai et al., 2015)

dLRRK^e03680

(Schaffner et al., 2019, Inoshita et al., 2017, Imai et al., 2015, Martin et al., 2014)

dLrrk^e03680

(Inoshita et al., 2022, Penney et al., 2016)

e03680

(Hernandez-Diaz et al., 2022, Matta et al., 2012, Kanao et al., 2010, Lin et al., 2010, Lee et al., 2007)

lrrk^e03680

(Dodson et al., 2014, Dodson et al., 2012)

Name Synonyms

Secondary FlyBase IDs

References (25)

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