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General Information
Symbol
Dmel\Gitf03586
Species
D. melanogaster
Name
FlyBase ID
FBal0186996
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Insertion components
PBac{WH}Gitf03586
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Gitex10/Gitf03586 flies have significant defects in climbing ability compared to controls. Postsynaptic glutamate receptor fields are enlarged in neuromuscular junctions of Gitex10/Gitf03586 third instar larvae. Gitex10/Gitf03586 third instar larval NMJs (type 1b) are similar to controls in terms of synaptic vesicle number, docked vesicle number, active zone size or T-bar morphology; however, stimulated mutants display an accumulation of endosomal structures and vacuoles (>80nm vesicles or cisternae). Gitex10/Gitf03586 third instar larval NMJs show significant reductions in evoked excitatory junctional currents (eEJCs) compared to controls (no difference in rise times or decay constants). Gitex10/Gitf03586 larval NMJs show defects in synaptic vesicle recycling, and specific defects in the slow phase of endocytosis and synaptic vesicle reacidification. Under conditions of elevated extracellular calcium, evoked neurotransmission of Gitex10/Gitf03586 larvae is similar to controls but FM1-43 uptake is significantly reduced (impaired endocytosis or synaptic vesicle recycling).

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Expression of GitScer\UAS.cPa or GitScer\UAS.cPa.T:Avic\GFP-EGFP driven by Scer\GAL4elav-C155 rescues climbing defects seen in Gitex10/Gitf03586 flies. Expression of GitScer\UAS.cPa driven by Scer\GAL4RapGAP1-OK6 rescues enlarged postsynaptic glutamate receptor fields and reduced eEJCs seen at the NMJ in Gitex10/Gitf03586 third instar larvae. Expression of GitScer\UAS.cPa driven by Scer\GAL4elav-C155 rescues increased endosome area per bouton seen at the NMJ in Gitex10/Gitf03586 third instar larvae. Expression of GitScer\UAS.cPa or GitScer\UAS.cPa.T:Avic\GFP-EGFP driven by Scer\GAL4RapGAP1-OK6 rescues synaptic vesicle recycling defects at the NMJ in Gitex10/Gitf03586 third instar larvae.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
CG16728f03586
Gitf03586
Name Synonyms
Secondary FlyBase IDs
    References (3)