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General Information
Symbol
Dmel\Vrp1f06715
Species
D. melanogaster
Name
FlyBase ID
FBal0187335
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Insertion components
PBac{WH}Vrp1f06715
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Longitudinal muscle development is not significantly disturbed in Vrp1f06715/Vrp1D30 transheterozygote embryos.

Homozygous embryos show defects in myoblast fusion.

Vrp1f06715 and Vrp1f06715/Df(2R)ED3943 mutants display a severe embryonic somatic muscle phenotype with strong muscle fusion defects.

In the visceral mesoderm of Vrp1f06715 embryos the founder cells and the fusion competent myoblasts appear to fuse despite the absence of Vrp1. The visceral mesoderm of Vrp1f06715 mutants appears slightly disorganised at early stages, however the development of the gut proceeds, and Vrp1f06715 mutants develop a gut structure with midgut constrictions at later stages.

At stage 12, in both Vrp1f06715 and control embryos the longitudinal muscles surround the circular musculature, and at later stage, both in mutants and controls, the longitudinal muscles form a characteristic longitudinal pattern, indicating that longitudinal muscle development is not affected in Vrp1f06715 mutants.

Some myoblast fusion takes place in Vrp1f06715 mutants, but growing myotubes are still surrounded by numerous unfused myoblasts at the end of embryogenesis.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

SCARΔ37;Vrp1f06715 double homozygous mutant embryos show normal gut constriction but aberrant morphology. Longitudinal visceral muscle founder cells show aberrant migration and severe muscle fusion defects are observed: mostly mononucleated and binucleated muscle cells are seen at late embryonic stages. These defects are weaker in SCARΔ37/SCARΔ37;Vrp1f06715/+ embryos (founder cell migration mostly normal though fusion defects are still observed) and not much improved in SCARΔ37/+;Vrp1f06715/Vrp1f06715 embryos (both founder cell migration and muscle fusion defects observed).

dock04723 enhances the myoblast fusion defects seen in Vrp1f06715 embryos.

WASp3 enhances the myoblast fusion defects seen in Vrp1f06715 embryos.

Loss of Vrp1 through a Vrp1f06715 mutant background does not affect fusion in Alk10 mutants, as mutant cells of the visceral mesoderm are able to both migrate and fuse with cells of the somatic mesoderm.

SCARΔ37 Vrp1f06715 double mutants exhibit a dramatically increased myoblast-fusion defect compared to the single mutants.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Expression of Vrp1Scer\UAS.fl.T:Hsap\MYC in fusion-competent myoblasts under the control of Scer\GAL4sns.PS fully rescues the muscle fusion defects found in Vrp1f06715 mutants.

Expression of Vrp1Scer\UAS.fl.T:Hsap\MYC in muscle cells under the control of Scer\GAL4twi.PG fully rescues the muscle fusion defects found in Vrp1f06715 mutants.

Expression of Vrp1Δ2xWH2.Scer\UAS.T:Hsap\MYC in fusion-competent myoblasts under the control of Scer\GAL4sns.PS fully rescues the muscle fusion defects found in Vrp1f06715 mutants.

Expression of Vrp1Δ2xWH2.Scer\UAS.T:Hsap\MYC in muscle cells under the control of Scer\GAL4twi.PG fully rescues the muscle fusion defects found in Vrp1f06715 mutants.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
Reported As
Symbol Synonym
CG13503f06715
Vrp1f06715
Name Synonyms
Secondary FlyBase IDs
    References (6)