FB2025_01 , released February 20, 2025
Allele: Dmel\ALiXf03094
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General Information
Symbol
Dmel\ALiXf03094
Species
D. melanogaster
Name
FlyBase ID
FBal0187416
Feature type
allele
Associated gene
Dmel\ALiX
Associated Insertion(s)
PBac{WH}ALiXf03094
Carried in Construct
Also Known As
alix1
Key Links
Genomic Maps

Allele class

amorphic allele - molecular evidence

amorphic allele - genetic evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - genetic evidence

(Eikenes et al., 2015)

amorphic allele - molecular evidence

(Eikenes et al., 2015)
Mutagen
piggyBac activity
(Gene Disruption Project members and Exelixis, 2005)
Progenitor genotype
Associated Insertion(s)
PBac{WH}ALiXf03094
Cytology
Description
Allele components
Component
Use(s)
Inserted element
PBac{WH}
Regulatory region(s)
UAS
Also carries
FRT
Mutations Mapped to the Genome
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      ALiXf03094 individuals show cytokinetic abscission defects in the germarium, as shown by the near complete absence of spectrosomes and reciprocal over-representation of cells without midbody or midbody rings.

      (Lie-Jensen et al., 2019)

      Approximately 60% of mutant egg chambers contain 32 germ cells. These egg chambers contain an oocyte with 5 ring canals.

      Mutant females mated to wild-type males show a strongly reduced rate of egg laying (approximately 10%) compared to controls. Less than 10% of the eggs laid hatch.

      Eggs laid by wild-type females mated to mutant males show a hatch rate of only approximately 40%.

      The fraction of mutant female germline stem cells (GSCs) which contain a spectrosome is smaller than wild type. The fraction of mutant GSC-cystoblast (CB) pairs with a midbody ring is reduced and mutant GSC-CB pairs with a midbody are only very rarely seen. More than half of the mutant female GSCs show abscission defects: linear or branched chains of GSCs are seen in approximately 10% and 30% of cases and approximately 30% of the cells are polyploid.

      Approximately 20% of mutant male germline stem cells are found interconnected to chains of daughter cells.

      (Eikenes et al., 2015)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      ALiXf03094 is rescued by Scer\GAL4nanos.PG/ALiXUASp.EGFP

      (Lie-Jensen et al., 2019)

      ALiXf03094 is rescued by ALiXCH322-119C06

      (Eikenes et al., 2015)

      ALiXf03094 is rescued by ALiXCH321-50C24

      (Eikenes et al., 2015)
      Partially rescued by

      ALiXf03094 is partially rescued by Scer\GAL4nanos.PG/ALiXP809V.P810D.UASp.EGFP

      (Lie-Jensen et al., 2019)

      ALiXf03094 is partially rescued by Scer\GAL4nanos.PG/ALiXUASp.EGFP

      (Eikenes et al., 2015)
      Not rescued by

      ALiXf03094 is not rescued by Scer\GAL4nanos.PG/ALiXL683D.UASp.EGFP

      (Lie-Jensen et al., 2019)

      ALiXf03094 is not rescued by Scer\GAL4nanos.PG/ALiXF676D.UASp.EGFP

      (Lie-Jensen et al., 2019)

      ALiXf03094 is not rescued by Scer\GAL4nanos.PG/ALiXF198D.UASp.EGFP

      (Eikenes et al., 2015)

      ALiXf03094 is not rescued by Scer\GAL4nanos.PG/ALiXI211D.UASp.EGFP

      (Eikenes et al., 2015)
      Comments

      Expression of ALiXScer\UAS.P\T.T:Avic\GFP-EGFP under the control of Scer\GAL4nos.PG partially rescues the germline stem cell abscission defects seen in ALiXf03094 females. The fraction of egg chambers containing 32 germ cells is also reduced.

      Expression of either ALiXF198D.Scer\UAS.P\T.T:Avic\GFP-EGFP or ALiXI211D.Scer\UAS.P\T.T:Avic\GFP-EGFP under the control of Scer\GAL4nos.PG fails to rescue the germline stem cell abscission defects seen in ALiXf03094 females. The 32-cell egg chamber phenotype is also not rescued.

      (Eikenes et al., 2015)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      ALiXf03094
      CG12876f03094
      alix1
      (Hermant et al., 2024, Mathieu et al., 2022, Lie-Jensen et al., 2019, Eikenes et al., 2015)
      Name Synonyms
      Secondary FlyBase IDs
        References (5)