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General Information
Symbol
Dmel\Ank2f00518
Species
D. melanogaster
Name
FlyBase ID
FBal0188204
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
ank2518, ank2null
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Insertion components
PBac{WH}Ank2f00518
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Larval muscle 4 neuromuscular junctions in Ank2XLΔ homozygotes display a significant decrease in the number of synaptic boutons, in parallel with bouton fusion and pronounced microtubule accumulation in the nerve terminal, as compared to controls; heterozygotes, however, do not show significant changes in synaptic bouton number, as compared to controls.

Ank2f00518/Ank2f00518 mutant larvae exhibit lethality, and neuromuscular junctions exhibit defects in synaptic morphology and stability, and defects in microtubule organization.

Expression of Ank2XL-Δall or Ank2XL-ΔC in a Ank2f00518/Ank2f00518 background in larvae results in neuromuscular junctions exhibiting defective microtubule organization and synapse morphology, and the type 1b axons innervating NMJs exhibit aberrant axonal microtubule accumulations, as compared to controls.

Expression of Ank2XL-5xR, Ank2XL-ΔR, or Ank2XL-20xR, in a Ank2f00518/Ank2f00518 background in larvae results in type 1b axons innervating NMJs exhibit aberrant axonal microtubule accumulations, as compared to controls.

Ank2f00518 homozygous mutants exhibit a synaptic retraction phenotype.

Homozygous and Ank2f00518/Df(3L)RM5-2 animals show severe synaptic retractions at 29[o]C of neuromuscular junctions.

Ank2f02001/Ank2f00518 animals die as third instar larvae/early pupae, and they show severe synaptic retractions at the neuromuscular junctions.

Ank2f00518/Df(3L)RM5-2 second instar larvae show defects in synaptic transmission and motoneuron excitability at the NMJ. Defects in in synapse morphology are also seen at the NMJ, with loss of the narrow interbouton regions and loss of discrete, evenly spaced boutons. The presynaptic microtubule cytoskeleton is severely disorganised.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Suppressed by
NOT suppressed by
Statement
Reference
Enhancer of
Statement
Reference
Suppressor of
NOT Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference

futschK68/futschK68 fails to suppress the defective synaptic stability of Ank2f00518/Ank2f00518 mutant larval neuromuscular junctions.

Presynaptic expression of CkIIαScer\UAS.N.T:Ivir\HA1 in Ank2f00518 homozygous mutants fails to alleviate the synaptic retraction phenotype.

Presynaptic expression of CkIIαK66M.Scer\UAS.N.T:Ivir\HA1 in Ank2f00518 homozygous mutants does not affect the synaptic retraction phenotype.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Fails to complement
Partially rescued by

Ank2f00518 is partially rescued by Ank2XL-5xR

Ank2f00518 is partially rescued by Ank2XL-5xR

Ank2f00518 is partially rescued by Ank2XL-ΔR

Ank2f00518 is partially rescued by Ank2XL-ΔR

Comments

Ank2XL-ΔR, Ank2XL-20xR or Ank2XL-5xR partially rescues the defective synapse morphology of Ank2f00518/Ank2f00518 mutant larvae.

Ank2+t82 fully rescues the lethality, synaptic morphology and stability, and microtubule organization of Ank2f00518/Ank2f00518 mutant larvae.

Ank2XL-Δall rescues the lethality of Ank2f00518/Ank2f00518 mutant larvae.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (6)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (9)