FlyBase Allele Report: Dmel\Act5C[UASp.R.GFP]

General Information

Symbol

Dmel\Act5C^UASp.R.GFP

Species

D. melanogaster

Name

FlyBase ID

FBal0188266

Feature type

allele

Associated gene

Dmel\Act5C

Associated Insertion(s)

Carried in Construct

P{UASp-GFP.Act5C}

Also Known As

UAS-Act5C-GFP, UAS-actin-GFP, UAS-act5c::GFP

Key Links

Transgenic product class

wild_type

(Roper et al., 2005)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Roper et al., 2005)

Mutagen

in vitro construct

(Roper et al., 2005)

Progenitor genotype

Carried in construct

P{UASp-GFP.Act5C}

Cytology

Description

UASp regulatory sequences drive expression of Act5C tagged with GFP at its N terminus.

(Roper et al., 2005)

Allele components

Component

Use(s)

Regulatory region(s)

UASp

binary expression system - regulatory region

Encoded product / tool

Act5C

Tagged with

GFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates synucleinopathy

modeled by Hsap\SNCA^QUAS.cOa

(Ordonez et al., 2018)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle, with Scer\GAL4^{VP16.nanos.UTR}

(Roper et al., 2005)

lethal, with Scer\GAL4^Mef2.PU

(Min et al., 2017)

Phenotype Manifest In

germline cyst, with Scer\GAL4^{VP16.nanos.UTR}

(Roper et al., 2005)

Detailed Description

Statement

Reference

The expression of Act5C^{Scer\UAS.P\T.R.T:Avic\GFP} under the control of Scer\GAL4^elav-C155 has no significant effect on the numbers of neurons or in the number of rod-shaped actin-rich inclusions in the adult brain, as compared to controls.

(Ordonez et al., 2018)

The expression of Act5C^{Scer\UAS.P\T.R.T:Avic\GFP} under the control of Scer\GAL4^Mef2.PU induces lethality before adulthood.

(Min et al., 2017)

When Act5C^{Scer\UAS.P\T.T:Avic\GFP} is driven by Scer\GAL4^{nos.UTR.T:Hsim\VP16} a failure in the formation of germline cysts with the correct number of germ cells is seen in a fraction of ovarioles.

(Roper et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Act5C^UASp.R.GFP, Scer\GAL4^Mef2.PU has lethal phenotype, non-suppressible by mst^JF03280, Scer\GAL4^Mef2.PU

(Min et al., 2017)

Enhancer of

Statement

Reference

Scer\GAL4^elav-C155/Act5C^UASp.R.GFP is an enhancer of abnormal locomotor behavior | adult stage phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Ordonez et al., 2018)

Scer\GAL4^elav-C155/Act5C^UASp.R.GFP is an enhancer of decreased cell number | adult stage phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Ordonez et al., 2018)

Phenotype Manifest In

Enhancer of

Statement

Reference

Scer\GAL4^elav-C155/Act5C^UASp.R.GFP is an enhancer of adult brain phenotype of Hsap\SNCA^QUAS.cOa, Ncra\QF^QF2.nSyb

(Ordonez et al., 2018)

Additional Comments

Genetic Interactions

Statement

Reference

The lethality induced by the expression of Act5C^{Scer\UAS.P\T.R.T:Avic\GFP} under the control of Scer\GAL4^Mef2.PU is not suppressed by the co-expression of mst^JF03280.

(Min et al., 2017)

Xenogenetic Interactions

Statement

Reference

The adult locomotor activity defects, the adult brain vacuolization, the decreased number of neurons (including TH-immunoreactive dopaminergic neurons) and the increased number of rod-shaped actin-rich inclusions in the adult brain resulting from the expression of Hsap\SNCA^{Ncra\QUAS.cOa} under the control of Ncra\QF^QF2.nSyb are enhanced by the additional expression of Act5C^{Scer\UAS.P\T.R.T:Avic\GFP} under the control of Scer\GAL4^elav-C155.

(Ordonez et al., 2018)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

9257

w^*; P{UASp-GFP.Act5C}3

9258

w^*; P{UASp-GFP.Act5C}2-1