The Npc1a2 mutant alone can be used to recapitulate developmental phenotypes, but as these flies are lethal it is not possible to use this model to look at adults. Overexpressing Npc1aScer\UAS.T:Avic\GFP-EYFP in the ring gland is sufficient to overcome this lethality, producing a model that can be used to examine phenotypes in various adult tissues.
Expression of NPC1Scer\UAS.T:Avic\GFP-EYFP in the ring and salivary glands, driven by Scer\GAL42-286, rescues the lethality of NPC12 mutants, allowing them to survive to adulthood. The subcellular accumulation of sterols in many tissues other than the ring gland is not reduced in the rescued mutants. Sterol accumulation is most severe in the Malpighian tubules, which show ultrastructural defects. Expression of NPC1Scer\UAS.T:Avic\GFP-EYFP in the ring gland, salivary glands, trachea, midgut and Malpighian tubules, driven by Scer\GAL4Feb36, also rescues NPC12 lethality, although some mutants still die as third instar larvae.