FlyBase Allele Report: Dmel\Dronc[L32]

General Information

Symbol

Dmel\Dronc^L32

Species

D. melanogaster

Name

FlyBase ID

FBal0189653

Feature type

allele

Associated gene

Dmel\Dronc

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

hypomorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Xu et al., 2005)

loss of function allele

(Arama et al., 2006)

Mutagen

ethyl methanesulfonate

(Arama et al., 2006, Xu et al., 2005)

Progenitor genotype

Cytology

Description

Nucleotide substitution: T?A.

(Bergman, 2007.4.12, Bergman, 2006.7.7)

Amino acid replacement: L24Q.

(Bergman, 2007.4.12)

Amino acid replacement: L25E.

(Arama et al., 2006, Xu et al., 2005)

Amino acid replacement: L25Q.

(Bergman, 2006.7.7)

FlyBase curator comment: the leucine at 25 is in fact mutated to a glutamine, rather than a glutamate residue.

(Arama et al., 2006, Xu et al., 2005)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:9,968,961..9,968,961 [+]

Nucleotide change:

T9968961A

Amino acid change:

L25Q | Dronc-PA

Reported amino acid change:

L32E

Comment:

The leucine at 25 is in fact mutated to a glutamine, rather than a glutamate residue.

(Xu et al., 2005)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of testicular cancer

CEA with Dronc^I29

(Napoletano et al., 2017)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

cystic bulge

(Arama et al., 2006)

cystic bulge (with Dronc^I29)

(Napoletano et al., 2017)

male germline cell (with Dronc^I24)

(Yacobi-Sharon et al., 2013)

male germline cell (with Dronc^I29)

(Yacobi-Sharon et al., 2013)

spermatid

(Arama et al., 2006)

spermatogonial cyst (with Dronc^I24)

(Yacobi-Sharon et al., 2013)

spermatogonium (with Dronc^I29)

(Napoletano et al., 2017)

testis | adult stage (with Dronc^I29)

(Napoletano et al., 2017)

waste bag

(Arama et al., 2006)

waste bag (with Dronc^I29)

(Napoletano et al., 2017)

Detailed Description

Statement

Reference

Dronc^I29/Dronc^L32 transheterozygotes exhibit a significant increase in the proportion of hyperplastic testes as compared to controls; these testes exhibit a significant decrease in necrosis at their apical tip (as shown by the number of spermatogonial cysts bearing Propidium Iodine-positive or TUNEL-positive cells), as compared to controls. During spermatogenesis, cystic bulges and waste bags are largely absent and actin investment cones in the individualization complex are uncoordinated, as compared to controls.

(Napoletano et al., 2017)

Nc^I24/Nc^L32 mutant males exhibit ~40% less spermatogonial cyst death compared to controls.

Nc^I29/Nc^L32 mutant males exhibit ~60% less spermatogonial cyst death compared to controls.

(Yacobi-Sharon et al., 2013)

Nc^L32 mutants display severe defects during the spermatid individualization process. The cystic bulges are frequently reduced in size or appear flat due to a failure in the appropriate collection of the cytoplasm of the spermatids . The retained cytoplasm is clearly visualized as a trail along the entire length of what was supposed to have been the post-individualized portion of the spermatids. Frequently a large portion of the spermatid cytoplasm is retained in a 'mini' cystic bulge structure, which often contains part of the individualization complex. Waste bags are also reduced in size.

(Arama et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Dronc^L32/Dronc^L32 is a suppressor of visible | somatic clone phenotype of hid^GMR.PG

(Xu et al., 2005)

Dronc^L32/Dronc^L32 is a suppressor of visible | somatic clone phenotype of rpr^GMR.PW

(Xu et al., 2005)

Phenotype Manifest In

Suppressor of

Statement

Reference

Dronc^I24/Dronc^L32 is a suppressor | partially of ovariole phenotype of Diap1^6B/Diap1⁸

(Xu et al., 2005)

Dronc^L32/Dronc^L32 is a suppressor of eye | somatic clone phenotype of hid^GMR.PG

(Xu et al., 2005)

Dronc^L32/Dronc^L32 is a suppressor of eye | somatic clone phenotype of rpr^GMR.PW

(Xu et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Dronc^I29/Dronc^L32 is rescued by Dronc^pro.UAS

(Napoletano et al., 2017)

Dronc^I29/Dronc^L32 is rescued by Dronc^pro.C-A.UAS

(Napoletano et al., 2017)

Dronc^I29/Dronc^L32 is rescued by Dronc^ΔN.UAS

(Napoletano et al., 2017)

Dronc^I29/Dronc^L32 is rescued by Dronc^ΔN.C-A.UAS

(Napoletano et al., 2017)

Partially rescued by

Dronc^I29/Dronc^L32 is partially rescued by Dronc^WT.5'3'UTR

(Napoletano et al., 2017)

Dronc^I29/Dronc^L32 is partially rescued by Dronc^C-A.5'3'UTR

(Napoletano et al., 2017)

Not rescued by

Dronc^I29/Dronc^L32 is not rescued by Dronc^CARD.UAS

(Napoletano et al., 2017)

Comments

Both the significant proportion of hyperplastic adult testes and the increased necrosis at the apical tip of the testes observed in Dronc^I29/Dronc^L32 transheterozygotes are rescued by the expression of Dronc^pro.Scer\UAS, Dronc^{pro.C-A.Scer\UAS}, Dronc^ΔN.Scer\UAS, or Dronc^{ΔN.C-A.Scer\UAS}, but not Dronc^{CARD.Scer\UAS}, under the control of Scer\GAL4^nos.PU. The significant proportion of hyperplastic adult testes is also rescued by the expression of either Dronc^WT.5'3'UTR or Dronc^C-A.5'3'UTR.

The sperm individualization defects observed in Dronc^I29/Dronc^L32 transheterozygotes are not rescued by the expression of either Dronc^WT.5'3'UTR or Dronc^C-A.5'3'UTR.

(Napoletano et al., 2017)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Selected as: a recessive suppressor of the W^GMR.PG eye ablation phenotype.

(Xu et al., 2005)

Selected as: a mutant that recessively suppresses the eye ablation phenotype caused by eye-specific overexpression of W.

(Arama et al., 2006)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Dronc^L32

Nc^L32

(Bergman, 2007.4.12, Bergman, 2006.7.7)

dronc^L32

(Napoletano et al., 2017, Yacobi-Sharon et al., 2013, Shapiro et al., 2008, Arama et al., 2006, Xu et al., 2005)

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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