Allele Dmel\br^Z3.Scer\UAS

General Information
Symbol	Dmel\br^Z3.Scer\UAS	Species	D. melanogaster
Name		FlyBase ID	FBal0189741
Feature type	allele	Associated gene	Dmel\br

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Schubiger et al., 2005, Zhou et al., 2009)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of cDNA clone "dm797" which encodes the br Z3 isoform. (Zhou et al., 2009) Construct: Scer\UAS regulatory sequences drive expression of br-Z3 isoform coding sequences. (Schubiger et al., 2005)
Carried in construct	P{UAS-br.Z3} (Schubiger et al., 2005, Costantino et al., 2008, Mirth et al., 2009, Zhou et al., 2009, Zhou et al., 2004, Xiang et al., 2010)
Cytology
Phenotypic Data
Phenotypic Class
	lethal, with Scer\GAL4^Feb211 (Zhou et al., 2004) lethal, with Scer\GAL4^P0206 (Zhou et al., 2004) lethal \| embryonic stage, with Scer\GAL4^elav-C155 (Zhou et al., 2009) lethal \| first instar larval stage, with Scer\GAL4^elav-C155 (Zhou et al., 2009) lethal \| pharate adult stage, with Scer\GAL4^Feb204 (Zhou et al., 2004) lethal \| pupal stage P7, with Scer\GAL4^Feb204 (Zhou et al., 2004) locomotor behavior defective \| first instar larval stage, with Scer\GAL4^elav-C155 (Zhou et al., 2009) locomotor behavior defective \| second instar larval stage, with Scer\GAL4^elav-C155 (Zhou et al., 2009) neuroanatomy defective \| adult stage, with Scer\GAL4^P2.4.Pdf (Zhou et al., 2009)
Phenotype Manifest In
	LNv neuron, with Scer\GAL4^P2.4.Pdf (Zhou et al., 2009) prothoracic gland, with Scer\GAL4^P0206 (Zhou et al., 2004)
Detailed Description
	Statement Reference Animals expressing br[Z3.Scer\UAS] under the control of Scer\GAL4[elav-C155] that hatch into larvae show normal morphology but sluggish movements. Most fail to feed and die during the first instar. Some larvae are able to feed and survive to the second instar, but they show little coordinated movement. Less than 1% of larvae expressing br[Z3.Scer\UAS] (using the P{UAS-br.Z3}216-1 line) under the control of Scer\GAL4[elav-C155] hatch, and they show reduced crawling even after stimulation. Unhatched embryos expressing br[Z3.Scer\UAS] under the control of Scer\GAL4[elav-C155] have no gross anatomical defects. Animals expressing br[Z3.Scer\UAS] under the control of Scer\GAL4[P2.4.Pdf] show abnormalities in the LN[[v]] neurons at metamorphosis, with the neurons in the mutant animals having a diffuse spray of neurites that extend to the midline (in contrast to the compact projection pattern of wild-type neurons which terminates well before the midline). (Zhou et al., 2009) Animals expressing br[Z3.Scer\UAS] under the control of Scer\GAL4[Feb211] undergo normal larval and pupal moults. However, these animals fail to eclose properly, with their abdomens remaining in the puparial cases. Most animals expressing br[Z3.Scer\UAS] under the control of Scer\GAL4[Feb204] form puparia. All of the prepupae successfully pupate, with approximately 18% of them arrested at stage P7, 12% arrested as pharate adults, 14% partially eclosed and 56% fully eclosed. Approximately 53% of larvae expressing br[Z3.Scer\UAS] under the control of Scer\GAL4[P0206] become permanent first instar larvae which survive for 4-6 days. Of those larvae that ecdyse to the second instar, 81% are permanently arrested in the second instar, 19% ecdyse to the third instar and 1% form "L2 prepupae" which are characterised by their uneverted, single club-shaped anterior spiracles and small mouthhooks with a few teeth (characteristic of wild-type second instar larvae). Expression of br[Z3.Scer\UAS] under the control of Scer\GAL4[P0206] causes degeneration of the prothoracic gland portion of the ring gland by 2 days after ecdysis to the second instar. The corpora allata are unaffected. 63% of animals expressing br[Z3.Scer\UAS] under the control of Scer\GAL4[12.1] remain as first or second instar larvae. (Zhou et al., 2004)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	br^Z3.Scer\UAS
Name Synonym
Secondary FlyBase IDs

References ( 6 )
Research paper	Xiang et al., 2010, Dev. Biol. 344(2): 800--808 br regulates the expression of the ecdysone biosynthesis gene npc1. [FBrf0211381] Mirth et al., 2009, Development 136(14): 2345--2353 The Ecdysone receptor controls the post-critical weight switch to nutrition-independent differentiation in Drosophila wing imaginal discs. [FBrf0208188] Zhou et al., 2009, Neural Dev. 4(1): 39 Temporal patterns of broad isoform expression during the development of neuronal lineages in Drosophila. [FBrf0209341] Costantino et al., 2008, PLoS Genet. 4(6): e1000102 A novel ecdysone receptor mediates steroid-regulated developmental events during the mid-third instar of Drosophila. [FBrf0205257] Schubiger et al., 2005, Development 132(23): 5239--5248 Ligand-dependent de-repression via EcR/USP acts as a gate to coordinate the differentiation of sensory neurons in the Drosophila wing. [FBrf0190319] Zhou et al., 2004, J. Exp. Biol. 207(7): 1151--1161 Overexpression of broad: a new insight into its role in the Drosophila prothoracic gland cells. [FBrf0174866]

Allele Dmel\brZ3.Scer\UAS

Allele Dmel\br^Z3.Scer\UAS