FB2025_01 , released February 20, 2025
Allele: Avic\GFPGr66a.3x.IRES.2x
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General Information
Symbol
Avic\GFPGr66a.3x.IRES.2x
Species
A. victoria
Name
FlyBase ID
FBal0189764
Feature type
allele
Associated gene
Avic\GFP
Associated Insertion(s)
Carried in Construct
P{Gr66a-GFP.IRES.GFP.IRES.GFP}
Also Known As
Gr66a-GFP, Gr66a-I-GFP, Gr66a-IRES-GFP, Gr66a-GFP-IRES-GFP-IRES-GFP
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Wang et al., 2004)
Progenitor genotype
Avic\GFP2x.EGFP.UAS
(Wang et al., 2004)
Carried in construct
P{Gr66a-GFP.IRES.GFP.IRES.GFP}
Cytology
Description

A 3.15kb fragment including the Gr66a regulatory drives expression of three copies of GFP separated by IRES sites.

(Wang et al., 2004)
Allele components
Component
Use(s)
Regulatory region(s)
Gr66a
Encoded product / tool
GFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      third instar larval stage
      larval gustatory receptor neuron of the dorsal pharyngeal sense organ | subset
      (Mishra et al., 2013)
      larval gustatory receptor neuron of the ventral pharyngeal sense organ | subset
      (Mishra et al., 2013)
      larval gnathal ganglion | restricted
      (Mishra et al., 2013)
      gustatory receptor neuron of terminal organ | subset
      (Mishra et al., 2013)
      high salt/bitter-sensing neuron | subset of terminal organ
      (Alves et al., 2014)
      gustatory receptor neuron of terminal organ dorsolateral group | subset
      (Alves et al., 2014)
      adult stage
      high salt/bitter-sensing neuron of labellar taste bristle
      (Miyamoto et al., 2012, Gordon and Scott, 2009)
      gustatory receptor neuron of labellar taste bristle | subset
      (Fischler et al., 2007)
      gustatory receptor neuron | subset of adult gnathal ganglion
      (Fischler et al., 2007)
      high salt/bitter-sensing neuron
      (Cameron et al., 2010)
      gustatory receptor neuron | subset of labellar taste bristle
      (Cameron et al., 2010)
      gustatory receptor neuron of proboscis
      (Wang et al., 2004)
      Additional Information
      Statement
      Reference

      AvicGFPGr66a.3x.IRES.2x is expressed in four gustatory receptor neurons of the terminal organ, which co-express ScerGAL4sano, a marker for salt-responsive neurons. Expression is also driven in two terminal organ neurons of the dorsal organ ganglion.

      (Alves et al., 2014)

      AvicGFPGr66a.3x.IRES.2x is expressed in gustatory receptor neurons of the dorsal and ventral the larval pharyngeal sense organ projecting to the embryonic/larval subesophageal ganglion.

      (Mishra et al., 2013)

      AvicGFPGr66a.3x.IRES.2x labels the bitter-sensing neurons (L2 GRN) of the taste bristles of the proboscis. Neurons labeled with AvicGFPGr66a.3x.IRES.2x do not overlap with expression of ScerGAL4ppk28.PC, which labels the water-sensing neurons.

      (Cameron et al., 2010)

      The dendrites of the AvicGFPGr66a.3x.IRES.2x bitter-sensitive neurons of gustatory sensilla and the ScerGAL4E49 labelled motor neurons are not in close proximity.

      (Gordon and Scott, 2009)

      AvicGFPGr66a.3x.IRES.2x is expressed in the bitter-sensitive gustatory receptor neurons of the labellar taste bristles of the proboscis. Expression of AvicGFPGr66a.3x.IRES.2x in the proboscis is distinct from the expression of ScerGAL4E409. The projection of the neurons labeled by AvicGFPGr66a.3x.IRES.2x in the subesophageal ganglion does not overlap with the projections labeled by ScerGAL4E409.

      (Fischler et al., 2007)
       
      Marker for
      high salt/bitter-sensing neuron
      (Jeong et al., 2013, Miyamoto et al., 2012, Cameron et al., 2010)
      Reflects expression of
      Gr66a
      (Alves et al., 2014, Miyamoto et al., 2012, Cameron et al., 2010, Gordon and Scott, 2009, Fischler et al., 2007, Wang et al., 2004)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Avic\GFPGr66a.3x.IRES.2x
      Name Synonyms
      Secondary FlyBase IDs
        References (19)