Allele Scer\GAL4^phm.PO

General Information
Symbol	Scer\GAL4^phm.PO	Species	S. cerevisiae
Name	phantom construct of O'Connor	FlyBase ID	FBal0189907
Feature type	allele	Associated gene	Scer\GAL4

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02	Controlled Vocabulary Terms prepupal stage P2 mouth hooks pupal stage feeding behavior defective visible
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Mirth et al., 2005, Fluegel et al., 2006)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference A ~1.1kb sequence from upstream of the phm translational start codon drives expression of Scer\GAL4. (Ono et al., 2006) Construct: A phm promoter drives expression of Scer\GAL4. (Mirth et al., 2005)
Carried in construct	P{phm-GAL4.O} (Mirth et al., 2005, Walker et al., 2006, Ono et al., 2006, Serpe and O'Connor, 2006, Fluegel et al., 2006, Gesualdi and Haerry, 2007, Kugler et al., 2011, Lin et al., 2011, Mirth et al., 2009, Rewitz et al., 2009, Niwa et al., 2010, Mazock et al., 2010, Tennessen et al., 2011, Cáceres et al., 2011, Sundram et al., 2012, Talamillo et al., 2008, Grillo et al., 2012, Ou et al., 2011)
Cytology
Phenotypic Data
Phenotypic Class
	cell size defective, with Pi3K92E^{Scer\UAS.T:Hsap\MYC} (Mirth et al., 2005) developmental rate defective, with Cyp6t3^GD5748 (Ou et al., 2011) developmental rate defective, with Cyp6t3^KK103702 (Ou et al., 2011) developmental rate defective, with dib^KK106954 (Ou et al., 2011) developmental rate defective, with Nos^Mac.Scer\UAS (Cáceres et al., 2011) developmental rate defective, with phl^GD9831 (Rewitz et al., 2009) developmental rate defective, with ph-p^KK108787 (Ou et al., 2011) developmental rate defective, with Pi3K92E^{Scer\UAS.T:Hsap\MYC} (Mirth et al., 2005) developmental rate defective, with Ras85D^GD12553 (Rewitz et al., 2009) developmental rate defective, with rl^GD4697 (Rewitz et al., 2009) developmental rate defective, with tor^GD14396 (Rewitz et al., 2009) developmental rate defective \| first instar larval stage, with Hr4^Scer\UAS.cQa (Ou et al., 2011) developmental rate defective \| larval stage, with Eip75B^{A.Scer\UAS.cCa} (Cáceres et al., 2011) developmental rate defective \| larval stage, with Hr46^NIG.12208R (Cáceres et al., 2011) developmental rate defective \| larval stage, with Nos^{dsRNA.Scer\UAS.IR-X} (Cáceres et al., 2011) developmental rate defective \| larval stage, with Ras85D^V12.Scer\UAS (Ou et al., 2011) developmental rate defective \| larval stage, with tsl^{dsRNA.Scer\UAS} (Grillo et al., 2012) developmental rate defective \| third instar larval stage, with smt3^{dsRNA.Scer\UAS} (Talamillo et al., 2008) eclosion defective, with Nos^{dsRNA.Scer\UAS.IR-X} (Cáceres et al., 2011) eclosion rhythm defective, with lark^{dsRNA.Scer\UAS.cSa} (Sundram et al., 2012) eclosion rhythm defective, with lark^{dsRNA.Scer\UAS.cSb} (Sundram et al., 2012) eclosion rhythm defective, with lark^NIG.8597R (Sundram et al., 2012) feeding behavior defective, with Cyp6t3^GD5748 (Ou et al., 2011) increased cell size, with Nos^{dsRNA.Scer\UAS.IR-X} (Cáceres et al., 2011) large body, with Cyp6t3^GD5748 (Ou et al., 2011) large body, with Cyp6t3^KK103702 (Ou et al., 2011) large body, with dib^KK106954 (Ou et al., 2011) large body, with rl^GD4697 (Rewitz et al., 2009) large body, with tor^GD14396 (Rewitz et al., 2009) large body \| larval stage, with Eip75B^{A.Scer\UAS.cCa} (Cáceres et al., 2011) large body \| larval stage, with Hr46^NIG.12208R (Cáceres et al., 2011) large body \| larval stage, with Nos^{dsRNA.Scer\UAS.IR-X} (Cáceres et al., 2011) large body \| pupal stage, with ph-p^KK108787 (Ou et al., 2011) lethal \| first instar larval stage, with Pten^Scer\UAS.cGb (Mirth et al., 2005) lethal \| first instar larval stage, with spok^{dsRNA.Scer\UAS} (Ono et al., 2006) lethal \| larval stage, with Nos^Mac.Scer\UAS (Cáceres et al., 2011) lethal \| larval stage, with β-Spec^{M.Scer\UAS.T:Hsap\MYC} (Mazock et al., 2010) lethal \| third instar larval stage, with smt3^{dsRNA.Scer\UAS} (Talamillo et al., 2008) photoperiod response variant, with Pi3K92E^{Scer\UAS.T:Hsap\MYC} (Mirth et al., 2005) small body \| larval stage, with Nos^Mac.Scer\UAS (Cáceres et al., 2011) small body \| pharate adult stage, with InR^Scer\UAS.cHa (Mirth et al., 2005) small body \| pupal stage, with Ras85D^V12.Scer\UAS (Ou et al., 2011) small body \| second instar larval stage, with Pi3K92E^{Scer\UAS.T:Hsap\MYC} (Mirth et al., 2005) small body \| third instar larval stage, with Pi3K92E^{Scer\UAS.T:Hsap\MYC} (Mirth et al., 2005) visible \| prepupal stage P2, with Cyp6t3^KK103702 (Ou et al., 2011)
Phenotype Manifest In
	embryonic/larval fat body, with Nos^{dsRNA.Scer\UAS.IR-X} (Cáceres et al., 2011) lipid particle, with smt3^{dsRNA.Scer\UAS} (Talamillo et al., 2008) mouth hooks, with Cyp6t3^KK103702 (Ou et al., 2011) mouth hooks, with dib^KK106954 (Ou et al., 2011) mouth hooks, with ph-p^KK108787 (Ou et al., 2011) prothoracic gland, with Nos^{dsRNA.Scer\UAS.IR-X} (Cáceres et al., 2011) prothoracic gland, with Pi3K92E^{Scer\UAS.T:Hsap\MYC} (Mirth et al., 2005) prothoracic gland, with Ras85D^V12.Scer\UAS (Ou et al., 2011) prothoracic gland, with smt3^{dsRNA.Scer\UAS} (Talamillo et al., 2008) pupa, with Cyp6t3^GD5748 (Ou et al., 2011) pupa, with Cyp6t3^KK103702 (Ou et al., 2011) pupa, with dib^KK106954 (Ou et al., 2011) pupa, with ph-p^KK108787 (Ou et al., 2011) pupa, with tor^GD14396 (Rewitz et al., 2009) puparium, with Pi3K92E^{Scer\UAS.T:Hsap\MYC} (Mirth et al., 2005) vesicle, with Nos^{dsRNA.Scer\UAS.IR-X} (Cáceres et al., 2011) vesicle, with Nos^Mac.Scer\UAS (Cáceres et al., 2011)
Detailed Description
	Statement Reference
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhanced by
Statement Reference Hr46^NIG.12208R, Scer\GAL4^phm.PO has developmental rate defective \| larval stage phenotype, non-enhanceable by Hr46^NIG.12208R, Scer\GAL4^phm.PO (Cáceres et al., 2011, Cáceres et al., 2011) Nos^{dsRNA.Scer\UAS.IR-X}, Scer\GAL4^phm.PO has developmental rate defective \| larval stage phenotype, non-enhanceable by Eip75B^{A.Scer\UAS.cCa}, Scer\GAL4^phm.PO (Cáceres et al., 2011, Cáceres et al., 2011) Nos^{dsRNA.Scer\UAS.IR-X}, Scer\GAL4^phm.PO has developmental rate defective \| larval stage phenotype, non-enhanceable by Hr46^NIG.12208R, Scer\GAL4^phm.PO (Cáceres et al., 2011, Cáceres et al., 2011)
Suppressed by
Statement Reference Nos^{dsRNA.Scer\UAS.IR-X}, Scer\GAL4^phm.PO has developmental rate defective \| larval stage phenotype, suppressible by Eip75B^GD1434, Scer\GAL4^phm.PO (Cáceres et al., 2011, Cáceres et al., 2011) Nos^{dsRNA.Scer\UAS.IR-X}, Scer\GAL4^phm.PO has developmental rate defective \| larval stage phenotype, suppressible by Hr46^Scer\UAS.cTa, Scer\GAL4^phm.PO (Cáceres et al., 2011, Cáceres et al., 2011) Nos^{dsRNA.Scer\UAS.IR-X}, Scer\GAL4^phm.PO has eclosion defective phenotype, suppressible by Eip75B^GD1434, Scer\GAL4^phm.PO (Cáceres et al., 2011, Cáceres et al., 2011) Nos^{dsRNA.Scer\UAS.IR-X}, Scer\GAL4^phm.PO has eclosion defective phenotype, suppressible by Hr46^Scer\UAS.cTa, Scer\GAL4^phm.PO (Cáceres et al., 2011, Cáceres et al., 2011) Nos^{dsRNA.Scer\UAS.IR-X}, Scer\GAL4^phm.PO has increased cell size phenotype, suppressible by Eip75B^GD1434, Scer\GAL4^phm.PO (Cáceres et al., 2011, Cáceres et al., 2011) Nos^{dsRNA.Scer\UAS.IR-X}, Scer\GAL4^phm.PO has increased cell size phenotype, suppressible by Hr46^Scer\UAS.cTa, Scer\GAL4^phm.PO (Cáceres et al., 2011, Cáceres et al., 2011) Nos^{dsRNA.Scer\UAS.IR-X}, Scer\GAL4^phm.PO has large body \| larval stage phenotype, suppressible by Eip75B^GD1434, Scer\GAL4^phm.PO (Cáceres et al., 2011, Cáceres et al., 2011) Nos^{dsRNA.Scer\UAS.IR-X}, Scer\GAL4^phm.PO has large body \| larval stage phenotype, suppressible by Hr46^Scer\UAS.cTa, Scer\GAL4^phm.PO (Cáceres et al., 2011, Cáceres et al., 2011) Ras85D^V12.Scer\UAS, Scer\GAL4^phm.PO has developmental rate defective \| larval stage phenotype, suppressible by Scer\GAL4^P0206/Hr4^Scer\UAS.cQa (Ou et al., 2011) Ras85D^V12.Scer\UAS, Scer\GAL4^phm.PO has small body \| pupal stage phenotype, suppressible by Scer\GAL4^P0206/Hr4^Scer\UAS.cQa (Ou et al., 2011) Scer\GAL4^phm.PO, tor^GD14396 has developmental rate defective phenotype, suppressible by Ras85D^V12.Scer\UAS, Scer\GAL4^phm.PO (Rewitz et al., 2009, Rewitz et al., 2009) Scer\GAL4^phm.PO, tor^GD14396 has large body phenotype, suppressible by Ras85D^V12.Scer\UAS, Scer\GAL4^phm.PO (Rewitz et al., 2009, Rewitz et al., 2009)
NOT suppressed by
Statement Reference Hr46^NIG.12208R, Scer\GAL4^phm.PO has developmental rate defective \| larval stage phenotype, non-suppressible by Hr46^NIG.12208R, Scer\GAL4^phm.PO (Cáceres et al., 2011, Cáceres et al., 2011) Nos^{dsRNA.Scer\UAS.IR-X}, Scer\GAL4^phm.PO has developmental rate defective \| larval stage phenotype, non-suppressible by Eip75B^{A.Scer\UAS.cCa}, Scer\GAL4^phm.PO (Cáceres et al., 2011, Cáceres et al., 2011) Nos^{dsRNA.Scer\UAS.IR-X}, Scer\GAL4^phm.PO has developmental rate defective \| larval stage phenotype, non-suppressible by Hr46^NIG.12208R, Scer\GAL4^phm.PO (Cáceres et al., 2011, Cáceres et al., 2011)
NOT Enhancer of
Statement Reference Eip75B^{A.Scer\UAS.cCa}, Scer\GAL4^phm.PO is a non-enhancer of developmental rate defective \| larval stage phenotype of Nos^{dsRNA.Scer\UAS.IR-X}, Scer\GAL4^phm.PO (Cáceres et al., 2011, Cáceres et al., 2011) Hr46^NIG.12208R, Scer\GAL4^phm.PO is a non-enhancer of developmental rate defective \| larval stage phenotype of Hr46^NIG.12208R, Scer\GAL4^phm.PO (Cáceres et al., 2011, Cáceres et al., 2011) Hr46^NIG.12208R, Scer\GAL4^phm.PO is a non-enhancer of developmental rate defective \| larval stage phenotype of Nos^{dsRNA.Scer\UAS.IR-X}, Scer\GAL4^phm.PO (Cáceres et al., 2011, Cáceres et al., 2011)
Suppressor of
Statement Reference Cyp6t3^Scer\UAS.cDa/Scer\GAL4^phm.PO is a suppressor of developmental rate defective phenotype of Hr4¹ (Ou et al., 2011) Eip75B^GD1434, Scer\GAL4^phm.PO is a suppressor of developmental rate defective \| larval stage phenotype of Nos^{dsRNA.Scer\UAS.IR-X}, Scer\GAL4^phm.PO (Cáceres et al., 2011, Cáceres et al., 2011) Eip75B^GD1434, Scer\GAL4^phm.PO is a suppressor of eclosion defective phenotype of Nos^{dsRNA.Scer\UAS.IR-X}, Scer\GAL4^phm.PO (Cáceres et al., 2011, Cáceres et al., 2011) Eip75B^GD1434, Scer\GAL4^phm.PO is a suppressor of increased cell size phenotype of Nos^{dsRNA.Scer\UAS.IR-X}, Scer\GAL4^phm.PO (Cáceres et al., 2011, Cáceres et al., 2011) Eip75B^GD1434, Scer\GAL4^phm.PO is a suppressor of large body \| larval stage phenotype of Nos^{dsRNA.Scer\UAS.IR-X}, Scer\GAL4^phm.PO (Cáceres et al., 2011, Cáceres et al., 2011) Hr46^Scer\UAS.cTa, Scer\GAL4^phm.PO is a suppressor of developmental rate defective \| larval stage phenotype of Nos^{dsRNA.Scer\UAS.IR-X}, Scer\GAL4^phm.PO (Cáceres et al., 2011, Cáceres et al., 2011) Hr46^Scer\UAS.cTa, Scer\GAL4^phm.PO is a suppressor of eclosion defective phenotype of Nos^{dsRNA.Scer\UAS.IR-X}, Scer\GAL4^phm.PO (Cáceres et al., 2011, Cáceres et al., 2011) Hr46^Scer\UAS.cTa, Scer\GAL4^phm.PO is a suppressor of increased cell size phenotype of Nos^{dsRNA.Scer\UAS.IR-X}, Scer\GAL4^phm.PO (Cáceres et al., 2011, Cáceres et al., 2011) Hr46^Scer\UAS.cTa, Scer\GAL4^phm.PO is a suppressor of large body \| larval stage phenotype of Nos^{dsRNA.Scer\UAS.IR-X}, Scer\GAL4^phm.PO (Cáceres et al., 2011, Cáceres et al., 2011) Ras85D^V12.Scer\UAS, Scer\GAL4^phm.PO is a suppressor of developmental rate defective phenotype of Scer\GAL4^phm.PO, tor^GD14396 (Rewitz et al., 2009, Rewitz et al., 2009) Ras85D^V12.Scer\UAS, Scer\GAL4^phm.PO is a suppressor of large body phenotype of Scer\GAL4^phm.PO, tor^GD14396 (Rewitz et al., 2009, Rewitz et al., 2009)
NOT Suppressor of
Statement Reference Eip75B^{A.Scer\UAS.cCa}, Scer\GAL4^phm.PO is a non-suppressor of developmental rate defective \| larval stage phenotype of Nos^{dsRNA.Scer\UAS.IR-X}, Scer\GAL4^phm.PO (Cáceres et al., 2011, Cáceres et al., 2011) Hr46^NIG.12208R, Scer\GAL4^phm.PO is a non-suppressor of developmental rate defective \| larval stage phenotype of Hr46^NIG.12208R, Scer\GAL4^phm.PO (Cáceres et al., 2011, Cáceres et al., 2011) Hr46^NIG.12208R, Scer\GAL4^phm.PO is a non-suppressor of developmental rate defective \| larval stage phenotype of Nos^{dsRNA.Scer\UAS.IR-X}, Scer\GAL4^phm.PO (Cáceres et al., 2011, Cáceres et al., 2011) Scer\GAL4^phm.PO/spok^Scer\UAS.cOa is a non-suppressor of lethal \| first instar larval stage phenotype of mld⁴⁴²⁵/mld⁴⁷ (Ono et al., 2006) spo^Scer\UAS.cOa/Scer\GAL4^phm.PO is a non-suppressor of lethal \| first instar larval stage phenotype of mld⁴⁴²⁵/mld⁴⁷ (Ono et al., 2006)
Phenotype Manifest In
Suppressed by
Statement Reference Ras85D^V12.Scer\UAS, Scer\GAL4^phm.PO has prothoracic gland phenotype, suppressible by Scer\GAL4^P0206/Hr4^Scer\UAS.cQa (Ou et al., 2011)
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	Scer\GAL4^phm.PO/daw^Scer\UAS.cGa rescues daw^aact1 (Gesualdi and Haerry, 2007) Scer\GAL4^phm.PO/Npc1a^Scer\UAS.cFa rescues Npc1a^57A (Fluegel et al., 2006) Scer\GAL4^phm.PO/tok^{Scer\UAS.cSa.T:Ivir\HA1} rescues tok^Δ2-41/tok^E1 (Serpe and O'Connor, 2006)
Fails to rescue	ERR^Scer\UAS.cTa, ERR², Scer\GAL4^phm.PO fails to rescue ERR¹ (Tennessen et al., 2011) Nf1^Scer\UAS.cWa/Scer\GAL4^phm.PO fails to rescue Nf1^unspecified (Walker et al., 2006)
Comments
Stocks ( 0 )

Notes on Origin
Discoverer	M. O'Connor

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	Scer\GAL4^phm.PO
Name Synonym	phantom construct of O'Connor (Mirth et al., 2005)
Secondary FlyBase IDs

References ( 18 )
Research paper	Grillo et al., 2012, Sci. Rep. 2: 762 Conserved and divergent elements in Torso RTK activation in Drosophila development. [FBrf0219757] Sundram et al., 2012, J. Biol. Rhythms 27(3): 183--195 Cellular Requirements for LARK in the Drosophila Circadian System. [FBrf0218481] Cáceres et al., 2011, Genes Dev. 25(14): 1476--1485 Nitric oxide coordinates metabolism, growth, and development via the nuclear receptor E75. [FBrf0214403] Kugler et al., 2011, Genetics 188(1): 127--139 The Putzig-NURF Nucleosome Remodeling Complex Is Required for Ecdysone Receptor Signaling and Innate Immunity in Drosophila melanogaster. [FBrf0213648] Lin et al., 2011, PLoS Genet. 7(12): e1002408 Drosophila Ribosomal Protein Mutants Control Tissue Growth Non-Autonomously via Effects on the Prothoracic Gland and Ecdysone. [FBrf0217066] Ou et al., 2011, PLoS Biol. 9(9): e1001160 Nuclear Receptor DHR4 Controls the Timing of Steroid Hormone Pulses During Drosophila Development. [FBrf0216318] Tennessen et al., 2011, Cell Metab. 13(2): 139--148 The Drosophila Estrogen-Related Receptor Directs a Metabolic Switch that Supports Developmental Growth. [FBrf0212927] Mazock et al., 2010, Mol. Biol. Cell 21(16): 2860--2868 Transgene Rescue Identifies an Essential Function for Drosophila {beta} Spectrin in the Nervous System and a Selective Requirement for Ankyrin-2-binding Activity. [FBrf0211527] Niwa et al., 2010, Development 137(12): 1991--1999 Non-molting glossy/shroud encodes a short-chain dehydrogenase/reductase that functions in the 'Black Box' of the ecdysteroid biosynthesis pathway. [FBrf0210924] Mirth et al., 2009, Development 136(14): 2345--2353 The Ecdysone receptor controls the post-critical weight switch to nutrition-independent differentiation in Drosophila wing imaginal discs. [FBrf0208188] Rewitz et al., 2009, Science 326(5958): 1403--1405 The insect neuropeptide PTTH activates receptor tyrosine kinase torso to initiate metamorphosis. [FBrf0209466] Talamillo et al., 2008, Development 135(9): 1659--1668 Smt3 is required for Drosophila melanogaster metamorphosis. [FBrf0205327] Gesualdi and Haerry, 2007, Fly 1(4): 212--221 Distinct signaling of Drosophila Activin/TGF-beta family members. [FBrf0205031] Fluegel et al., 2006, Genetics 172(1): 185--196 Mutations of a Drosophila NPC1 gene confer sterol and ecdysone metabolic defects. [FBrf0190720] Ono et al., 2006, Dev. Biol. 298(2): 555--570 Spook and Spookier code for stage-specific components of the ecdysone biosynthetic pathway in Diptera. [FBrf0200145] Serpe and O'Connor, 2006, Development 133(24): 4969--4979 The metalloprotease tolloid-related and its TGF-beta-like substrate Dawdle regulate Drosophila motoneuron axon guidance. [FBrf0194328] Walker et al., 2006, Genes Dev. 20(23): 3311--3323 Reduced growth of Drosophila neurofibromatosis 1 mutants reflects a non-cell-autonomous requirement for GTPase-Activating Protein activity in larval neurons. [FBrf0193208] Mirth et al., 2005, Curr. Biol. 15(20): 1796--1807 The role of the prothoracic gland in determining critical weight for metamorphosis in Drosophila melanogaster. [FBrf0190035]

Allele Scer\GAL4phm.PO

Allele Scer\GAL4^phm.PO