Allele Dmel\Sh^{SDN.Scer\UAS.T:Avic\GFP-GL}

General Information
Symbol	Dmel\Sh^{SDN.Scer\UAS.T:Avic\GFP-GL}	Species	D. melanogaster
Name		FlyBase ID	FBal0189960
Feature type	allele	Associated gene	Dmel\Sh

Allele class
Mutagen	in vitro construct - deletion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - deletion (Mosca et al., 2005)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	Sh^{EKO.Scer\UAS.T:Avic\GFP-GL} (Mosca et al., 2005)
Nature of the lesion	Statement Reference Construct: All Sh sequence 3' to the S1 transmembrane helix (residue 246 and C-wards) have been deleted from Sh^{EKO.Scer\UAS.T:Avic\GFP-GL}. (Mosca et al., 2005)
Carried in construct	P{UAS-SDN.GFP} (Mosca et al., 2005)
Tagged with	T:Avic\GFP^GL (Mosca et al., 2005)
Cytology
Phenotypic Data
Phenotypic Class
	hyperactive \| RU486 conditional, with Scer\GAL4^{elav.Switch.PO} (Mosca et al., 2005) hyperactive \| RU486 conditional, with Scer\GAL4^{Mhc.Switch.PO} (Mosca et al., 2005) locomotor behavior defective \| RU486 conditional, with Scer\GAL4^{Mhc.Switch.PO} (Mosca et al., 2005) neurophysiology defective \| RU486 conditional, with Scer\GAL4^{elav.Switch.PO} (Mosca et al., 2005) neurophysiology defective \| RU486 conditional, with Scer\GAL4^{Mhc.Switch.PO} (Mosca et al., 2005) uncoordinated \| RU486 conditional, with Scer\GAL4^{elav.Switch.PO} (Mosca et al., 2005) uncoordinated \| RU486 conditional, with Scer\GAL4^{Mhc.Switch.PO} (Mosca et al., 2005) viable \| RU486 conditional, with Scer\GAL4^{elav.Switch.PO} (Mosca et al., 2005) viable \| RU486 conditional, with Scer\GAL4^{Mhc.Switch.PO} (Mosca et al., 2005)
Phenotype Manifest In
	muscle fiber \| larval stage \| RU486 conditional, with Scer\GAL4^{Mhc.Switch.PO} (Mosca et al., 2005)
Detailed Description
	Statement Reference When Sh^{SDN.Scer\UAS.T:Avic\GFP-GL} is driven by Scer\GAL4^{Mhc.Switch.PO} or Scer\GAL4^{elav.Switch.PO}, and the animals exposed to RU486, no significant reduction in viability is seen. When Sh^{SDN.Scer\UAS.T:Avic\GFP-GL} is driven by Scer\GAL4^{Mhc.Switch.PO} , and the animals exposed to RU486, the voltage-activated transient K⁺ current (I_A) in the larval muscle fibres is potently suppressed. I_K is unaffected. The frequency of spontaneous synaptic activity in Sh^{SDN.Scer\UAS.T:Avic\GFP-GL}, Scer\GAL4^{elav.Switch.PO} mutants exposed to RU486, is nearly twice that of wild-type. Excitatory postsynaptic potential (EPSP) duration is increased by ~49% when Scer\GAL4^{elav.Switch.PO} is driven by Scer\GAL4^{elav.Switch.PO} and exposed to RU486. When driven by Scer\GAL4^{Mhc.Switch.PO}, a 37% increase is seen. EPSP amplitude is unaffected. Mutant adults exhibit both leg shaking and wing scissoring under ether anaesthetization, when driven by Scer\GAL4^{Mhc.Switch.PO} or Scer\GAL4^{Mhc.Switch.PO} and exposed to RU486. This behaviour persists in severed legs. When Sh^{SDN.Scer\UAS.T:Avic\GFP-GL} is driven by Scer\GAL4^{Mhc.Switch.PO} mutant larvae (also exposed to RU486) crawl more slowly than wild-type. This affect is not seen if driven by Scer\GAL4^{elav.Switch.PO}. When Sh^{SDN.Scer\UAS.T:Avic\GFP-GL} is driven by Scer\GAL4^{elav.Switch.PO} or Scer\GAL4^{Mhc.Switch.PO} and mutants are exposed to RU486 no effect is seen on arborisation. (Mosca et al., 2005)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference Scer\GAL4^{elav.Switch.PO}, Sh^{SDN.Scer\UAS.T:Avic\GFP-GL} has hyperactive \| RU486 conditional phenotype, suppressible by mle^nap-ts1 (Mosca et al., 2005) Scer\GAL4^{elav.Switch.PO}, Sh^{SDN.Scer\UAS.T:Avic\GFP-GL} has uncoordinated \| RU486 conditional phenotype, suppressible by mle^nap-ts1 (Mosca et al., 2005)
Phenotype Manifest In
Other
Statement Reference Scer\GAL4^{elav.Switch.PO}, Sh^{SDN.Scer\UAS.T:Avic\GFP-GL}, eag¹ has NMJ bouton \| supernumerary phenotype (Mosca et al., 2005, Mosca et al., 2005)
Additional Comments
Genetic Interactions
Statement Reference When Sh^{SDN.Scer\UAS.T:Avic\GFP-GL} is driven by Scer\GAL4^{Mhc.Switch.PO} in a eag¹ and mutants are exposed to RU486 no effect is seen on arborisation. If driven by Scer\GAL4^{elav.Switch.PO} in a eag¹ background synaptic arbor growth is significantly increased. The mean bouton count in NMJ boutons is almost doubled in these mutants. (Mosca et al., 2005)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Sh^{SDN.Scer\UAS.T:Avic\GFP-GL}
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Mosca et al., 2005, Proc. Natl. Acad. Sci. U.S.A. 102(9): 3477--3482 Dissection of synaptic excitability phenotypes by using a dominant-negative Shaker K+ channel subunit. [FBrf0191470]

Allele Dmel\ShSDN.Scer\UAS.T:Avic\GFP-GL

Allele Dmel\Sh^{SDN.Scer\UAS.T:Avic\GFP-GL}