Allele Hsap\PLCD1^{βTub85D.T:Avic\GFP-EGFP}

General Information
Symbol	Hsap\PLCD1^{βTub85D.T:Avic\GFP-EGFP}	Species	H. sapiens
Name		FlyBase ID	FBal0189987
Feature type	allele	Associated gene	Hsap\PLCD1

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - coding region fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - coding region fusion (Wong, 2005) in vitro construct - regulatory fusion (Wong, 2005)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: A βTub85D promoter drives expression of T:Avic\GFP^EGFP, fused to the pleckstrin homology (PH) domain of Hsap\PLCD1. (Wong, 2005)
Carried in construct	P{βTub85D-PLCD1.GFP} (Wong, 2005, Wei et al., 2008, Goldbach et al., 2010, Fabian et al., 2010, Montembault et al., 2010)
Tagged with	T:Avic\GFP^EGFP (Wong et al., 2005, Wong, 2005)
Cytology
Phenotypic Data
Phenotypic Class
	cytokinesis defective \| male (Wong, 2005)
Phenotype Manifest In
	spermatid & nucleus \| supernumerary (Wong, 2005)
Detailed Description
	Statement Reference Expression of the Hsap\PLCD1^{βTub85D.T:Avic\GFP-EGFP} transgene causes a dramatic cytokinesis defect, with 47.8-72% of spermatids containing multiple nuclei. The majority of the multinucleate cells contain three or four nuclei. (Wong, 2005)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

Comments
	Used to titrate out PIP2. (Wong, 2005)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Hsap\PLCD1^{βTub85D.T:Avic\GFP-EGFP}
Name Synonym
Secondary FlyBase IDs

References ( 6 )
Research paper	Fabian et al., 2010, Mol. Biol. Cell 21(9): 1546--1555 Phosphatidylinositol 4,5-bisphosphate directs spermatid cell polarity and exocyst localization in Drosophila. [FBrf0210667] Goldbach et al., 2010, Mol. Biol. Cell 21(9): 1482--1493 Stabilization of the actomyosin ring enables spermatocyte cytokinesis in Drosophila. [FBrf0210683] Montembault et al., 2010, J. Cell Biol. 191(7): 1351--1365 Nessun Dorma, a novel centralspindlin partner, is required for cytokinesis in Drosophila spermatocytes. [FBrf0212581] Wei et al., 2008, J. Cell Sci. 121(7): 1076--1084 Depletion of plasma membrane PtdIns(4,5)P2 reveals essential roles for phosphoinositides in flagellar biogenesis. [FBrf0204429] Wong et al., 2005, Curr. Biol. 15(15): 1401--1406 PIP2 hydrolysis and calcium release are required for cytokinesis in Drosophila spermatocytes. [FBrf0187340]
Supplementary material	Wong, 2005, Curr. Biol. 15(15): [title not yet available] [FBrf0191781]

Allele Hsap\PLCD1βTub85D.T:Avic\GFP-EGFP

Allele Hsap\PLCD1^{βTub85D.T:Avic\GFP-EGFP}