Allele Dmel\Duox^{dsRNA.Scer\UAS}

General Information
Symbol	Dmel\Duox^{dsRNA.Scer\UAS}	Species	D. melanogaster
Name		FlyBase ID	FBal0190061
Feature type	allele	Associated gene	Dmel\Duox

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - RNAi
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Ha et al., 2005, Ha et al., 2005) in vitro construct - RNAi (Ha et al., 2005, Ha et al., 2005)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS regulatory sequences drive expression of a head-to-tail inverted repeat of a 510bp fragment encoding amino acids 976 to 1145 of Duox. The inverted repeats are separated by an intronic spacer. (Ha et al., 2005)
Carried in construct	P{UAS-Duox.RNAi} (Ha et al., 2005, Ha et al., 2005, Ha et al., 2009, Ha et al., 2009, Ryu et al., 2006, Ha et al., 2009, Anh et al., 2011, Chakrabarti et al., 2012)
Cytology
Phenotypic Data
Phenotypic Class
	immune response defective, with Scer\GAL4^cad-em459 (Ha et al., 2005, Ha et al., 2005) immune response defective, with Scer\GAL4^da.G32 (Ha et al., 2005, Ha et al., 2005, Ha et al., 2009, Ha et al., 2009) increased cell death \| third instar larval stage, with Scer\GAL4^en.PU (Anh et al., 2011) lethal, with Scer\GAL4^Act5C.PU (Anh et al., 2011) lethal, with Scer\GAL4^tub.PU (Anh et al., 2011) lethal \| pupal stage, with Scer\GAL4^c179 (Anh et al., 2011)
Phenotype Manifest In
	ommatidium, with Scer\GAL4^GMR.PU (Anh et al., 2011) wing, with Scer\GAL4^Bx-MS1096 (Anh et al., 2011) wing, with Scer\GAL4^en.PU (Anh et al., 2011) wing vein, with Scer\GAL4^en.PU (Anh et al., 2011)
Detailed Description
	Statement Reference Expression of Duox[dsRNA.Scer\UAS] using Scer\GAL4[GMR.PU] results in dark circles inside ommatidia. Expression of Duox[dsRNA.Scer\UAS] using Scer\GAL4[ey.PU], Scer\GAL4[tey-5053A] or Scer\GAL4[rho.PL] does not produce detectable phenotypes. Expression of Duox[dsRNA.Scer\UAS] using Scer\GAL4[en.PU] or Scer\GAL4[Bx-MS1096] results in held-out and fragile wings. With Scer\GAL4[en.PU], wing veins are also indistinct and the posterior compartment is paler. In flies containing 2 copies of both Scer\GAL4[en.PU] and Duox[dsRNA.Scer\UAS], the posterior area of wings appears lightly crinkled and damaged around 3 days after hatching. After 6 days, the damage increases in severity. Penetrance is 100% after 6 days. Expression of Duox[dsRNA.Scer\UAS] using Scer\GAL4[en.PU] eliminates reactive oxygen species in the posterior compartment of the adult wing. Expression of Duox[dsRNA.Scer\UAS] using Scer\GAL4[en.PU] results in increased apoptosis in the posterior compartment of the wing disc. The amino acid composition of and the average amount of amino acids in Duox[dsRNA.Scer\UAS], Scer\GAL4[en.PU] adult wings is similar to controls. However, the catechol component (which is an important precursor in cuticular sclerotization and melanization) in the mutant wings is significantly lower than in controls wings. Mutant wings also show a decreased dityrosine component in hydrolysis samples, indicating reduced dityrosine linkages in the cuticle. (Anh et al., 2011) Flies expressing Duox[dsRNA.Scer\UAS] under the control of Scer\GAL4[da.G32] show a normal lifespan on dead yeast medium, but show reduced survival compared to controls on live yeast medium. Flies expressing Duox[dsRNA.Scer\UAS] under the control of Scer\GAL4[da.G32] and raised on live yeast medium for 1 hour have a similar number of living yeast in the gut (assayed as the number of colony-forming units (CFUs) from homogenates of surface-sterilized intestines) immediately after the live yeast feeding period, but in contrast to wild-type flies (where the number of initial CFUs decreases to a low level starting from 24 hours after ingestion), the initial CFUs in the mutant gut are maintained for 48 hours after ingestion and increase steadily thereafter. (Ha et al., 2009) Flies expressing Duox[dsRNA.Scer\UAS] under the control of Scer\GAL4[da.G32] show reduced survival after gut infection (via feeding) with Erwinia carotovora carotovora strain 15 compared to control flies. (Ha et al., 2009) Flies expressing Duox^{dsRNA.Scer\UAS} under the control of Scer\GAL4^da.G32 or Scer\GAL4^cad-em459 show a reduced survival rate compared to control flies when naturally infected with "Ecc15" (P.carotovorum.carotovorum). Flies expressing Duox^{dsRNA.Scer\UAS} under the control of Scer\GAL4^c564 show a normal survival rate when naturally infected with "Ecc15" (P.carotovorum.carotovorum). Ingested "Ecc15" (P.carotovorum.carotovorum) bacteria persist and are able to proliferate throughout the intestinal tracts of flies expressing Duox^{dsRNA.Scer\UAS} under the control of Scer\GAL4^da.G32, in contrast to normal flies. (Ha et al., 2005) Flies expressing Duox^{dsRNA.Scer\UAS} under the control of Scer\GAL4^cad-em459 show a reduced survival rate compared to control flies when naturally infected with E.coli, S.cerevisiae, M.luteus or S.typhimurium. Flies expressing Duox^{dsRNA.Scer\UAS} under the control of Scer\GAL4^da.G32 show a reduced survival rate compared to control flies when naturally infected with "Ecc15" (P.carotovorum.carotovorum). (Ha et al., 2005)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference Duox^{dsRNA.Scer\UAS}, Scer\GAL4^cad-em459 has immune response defective phenotype, suppressible by Hsap\DUOX1^Scer\UAS.cHa, Scer\GAL4^cad-em459 (Ha et al., 2005) Duox^{dsRNA.Scer\UAS}, Scer\GAL4^cad-em459 has immune response defective phenotype, suppressible by Hsap\DUOX2^Scer\UAS.cHa, Scer\GAL4^cad-em459 (Ha et al., 2005) Duox^{dsRNA.Scer\UAS}, Scer\GAL4^cad-em459 has immune response defective phenotype, suppressible by Irc^{dsRNA.Scer\UAS}, Scer\GAL4^cad-em459 (Ha et al., 2005) Duox^{dsRNA.Scer\UAS}, Scer\GAL4^en.PU has increased cell death \| third instar larval stage phenotype, suppressible by Df(3L)H99/+ (Anh et al., 2011)
Suppressor of
Statement Reference Duox^{dsRNA.Scer\UAS}, Scer\GAL4^cad-em459 is a suppressor of immune response defective phenotype of Irc^{dsRNA.Scer\UAS}, Scer\GAL4^cad-em459 (Ha et al., 2005) Duox^{dsRNA.Scer\UAS}, Scer\GAL4^da.G32 is a suppressor of increased cell death phenotype of Mkp3^GD8219, Scer\GAL4^da.G32 (Ha et al., 2009) Duox^{dsRNA.Scer\UAS}, Scer\GAL4^da.G32 is a suppressor of short lived phenotype of Mkp3^GD8219, Scer\GAL4^da.G32 (Ha et al., 2009)
Phenotype Manifest In
Suppressed by
Statement Reference Duox^{dsRNA.Scer\UAS}, Scer\GAL4^en.PU has wing phenotype, suppressible by Df(3L)H99/+ (Anh et al., 2011) Duox^{dsRNA.Scer\UAS}, Scer\GAL4^en.PU has wing vein phenotype, suppressible by Df(3L)H99/+ (Anh et al., 2011)
Suppressor of
Statement Reference Duox^{dsRNA.Scer\UAS}, Scer\GAL4^da.G32 is a suppressor of adult posterior midgut epithelium phenotype of Mkp3^GD8219, Scer\GAL4^da.G32 (Ha et al., 2009)
Additional Comments
Genetic Interactions
Statement Reference Df(3L)H99/+ suppresses the adult wing phenotype and the increased apoptosis in posterior wing discs seen in Duox[dsRNA.Scer\UAS], Scer\GAL4[en.PU] flies. (Anh et al., 2011) The shortened lifespan and increased apoptosis seen in the posterior midgut of flies expressing Mkp3[GD8219] under the control of Scer\GAL4[da.G32] is significantly rescued by co-expression of Duox[dsRNA.Scer\UAS]. (Ha et al., 2009) Flies co-expressing Irc^{dsRNA.Scer\UAS} and Duox^{dsRNA.Scer\UAS} under the control of Scer\GAL4^cad-em459 show a normal survival rate when naturally infected with "Ecc15" (P.carotovorum.carotovorum). (Ha et al., 2005)
Xenogenetic Interactions
Statement Reference The survival rates of flies expressing Duox^{dsRNA.Scer\UAS} under the control of Scer\GAL4^cad-em459 after natural infection with "Ecc15" (P.carotovorum.carotovorum) are markedly improved if they co-express either Hsap\DUOX1^Scer\UAS.cHa or Hsap\DUOX2^Scer\UAS.cHa. (Ha et al., 2005)
Complementation & Rescue Data
Rescued by	Duox^{dsRNA.Scer\UAS} is rescued by Duox^Scer\UAS.cHa/Scer\GAL4^cad-em459 (Ha et al., 2005) Duox^{dsRNA.Scer\UAS} is rescued by Scer\GAL4^da.G32/Duox^Scer\UAS.cHa (Ha et al., 2005, Ha et al., 2009)
Not rescued by	Duox^{dsRNA.Scer\UAS} is not rescued by Duox^{ΔPHD.Scer\UAS}/Scer\GAL4^cad-em459 (Ha et al., 2005) Duox^{dsRNA.Scer\UAS} is not rescued by Duox^{ΔPHD.Scer\UAS}/Scer\GAL4^da.G32 (Ha et al., 2005)
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Duox^{dsRNA.Scer\UAS}
Name Synonym
Secondary FlyBase IDs

References ( 8 )
Research paper	Chakrabarti et al., 2012, Cell Host Microbe 12(1): 60--70 Infection-induced host translational blockage inhibits immune responses and epithelial renewal in the Drosophila gut. [FBrf0218984] Anh et al., 2011, J. Biol. Chem. 286(38): 33244--33251 Essential role of duox in stabilization of Drosophila wing. [FBrf0215569] Ha et al., 2009, Nat. Immunol. 10(9): 949--957 Coordination of multiple dual oxidase-regulatory pathways in responses to commensal and infectious microbes in drosophila gut. [FBrf0208708] Ha et al., 2009, Dev. Cell 16(3): 386--397 Regulation of DUOX by the Galphaq-phospholipase Cbeta-Ca2+ pathway in Drosophila gut immunity. [FBrf0207552] Ryu et al., 2006, EMBO J. 25(15): 3693--3701 An essential complementary role of NF-kappaB pathway to microbicidal oxidants in Drosophila gut immunity. [FBrf0193652] Ha et al., 2005, Science 310(5749): 847--850 A direct role for dual oxidase in Drosophila gut immunity. [FBrf0190609]
Supplementary material	Ha et al., 2009, Nat. Immunol. 10(9): Online methods. [FBrf0209482] Ha et al., 2005, Science 310(5749): A direct role for dual oxidase in Drosophila gut immunity. [FBrf0191535]

Allele Dmel\DuoxdsRNA.Scer\UAS

Allele Dmel\Duox^{dsRNA.Scer\UAS}