FlyBase Allele Report: Dmel\tio[UAS.cLa]

General Information

Symbol

Dmel\tio^UAS.cLa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Laugier

FlyBase ID

FBal0190154

Feature type

allele

Associated gene

Dmel\tio

Associated Insertion(s)

Carried in Construct

P{UAS-tio.L}

Also Known As

UAS-tio

Key Links

Transgenic product class

wild_type

(Laugier et al., 2005)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Laugier et al., 2005)

Mutagen

in vitro construct

(Laugier et al., 2005)

Progenitor genotype

Carried in construct

P{UAS-tio.L}

Cytology

Description

UASt sequences drive expression of an EcoRI fragment of tio.

(Laugier et al., 2005)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

tio

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Alzheimer's disease 1

modeled by Hsap\APP^{Aβ42.UAS.cUa}

(Moran et al., 2013)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^c329b

(Datta et al., 2009)

visible, with Scer\GAL4^C833

(Datta et al., 2009)

visible, with Scer\GAL4^cb26

(Datta et al., 2009)

visible, with Scer\GAL4^cb41

(Datta et al., 2009)

visible, with Scer\GAL4^dpp.PS

(Datta et al., 2009)

visible, with Scer\GAL4^GMR.PF

(Datta et al., 2009)

visible, with Scer\GAL4^MJ33a

(Datta et al., 2009)

visible, with Scer\GAL4^Ser.PGF

(Datta et al., 2009)

visible, with Scer\GAL4^T98

(Datta et al., 2009)

visible | homeotic, with Scer\GAL4^69B

(Laugier et al., 2005)

Phenotype Manifest In

antennal disc | ectopic | third instar larval stage, with Scer\GAL4^c329b

(Datta et al., 2009)

antennal disc | ectopic | third instar larval stage, with Scer\GAL4^C833

(Datta et al., 2009)

antennal disc | ectopic | third instar larval stage, with Scer\GAL4^cb26

(Datta et al., 2009)

antennal disc | ectopic | third instar larval stage, with Scer\GAL4^cb41

(Datta et al., 2009)

antennal disc | ectopic | third instar larval stage, with Scer\GAL4^dpp.PS

(Datta et al., 2009)

antennal disc | ectopic | third instar larval stage, with Scer\GAL4^MJ33a

(Datta et al., 2009)

antennal disc | ectopic | third instar larval stage, with Scer\GAL4^Ser.PGF

(Datta et al., 2009)

antennal disc | ectopic | third instar larval stage, with Scer\GAL4^T98

(Datta et al., 2009)

denticle | heat sensitive, with Scer\GAL4^69B

(Laugier et al., 2005)

eye | ectopic, with Scer\GAL4^c329b

(Datta et al., 2009)

eye | ectopic, with Scer\GAL4^C833

(Datta et al., 2009)

eye | ectopic, with Scer\GAL4^cb26

(Datta et al., 2009)

eye | ectopic, with Scer\GAL4^cb41

(Datta et al., 2009)

eye | ectopic, with Scer\GAL4^dpp.PS

(Datta et al., 2009)

eye | ectopic, with Scer\GAL4^MJ33a

(Datta et al., 2009)

eye | ectopic, with Scer\GAL4^Ser.PGF

(Datta et al., 2009)

eye | ectopic, with Scer\GAL4^T98

(Datta et al., 2009)

eye photoreceptor cell, with Scer\GAL4^GMR.PF

(Datta et al., 2009)

larval labial segment, with Scer\GAL4^69B

(Laugier et al., 2005)

T1 beard | ectopic, with Scer\GAL4^69B

(Laugier et al., 2005)

Detailed Description

Statement

Reference

tio^Scer\UAS.cLa under the control of Scer\GAL4^GMR.PF results in roughening of the posterior portion of the adult eye as a result of nonspecific loss of photoreceptor cells within each ommatidium.

tio^Scer\UAS.cLa under the control of Scer\GAL4^dpp.PS induces ectopic eye formation in the third-instar antennal imaginal disc and adult. The antennal discs have a distorted shape and are significantly larger in size than wildtype.

tio^Scer\UAS.cLa under the control of Scer\GAL4^cb26 induces ectopic eye formation in the third-instar antennal imaginal disc and adult. The antennal discs have a distorted shape and are significantly larger in size than wildtype.

tio^Scer\UAS.cLa under the control of Scer\GAL4^cb41 induces ectopic eye formation in the third-instar antennal imaginal disc and adult. The antennal discs have a distorted shape and are significantly larger in size than wildtype.

tio^Scer\UAS.cLa under the control of Scer\GAL4^Ser.PGF often induces multiple ectopic eyes within a single third-instar antennal imaginal disc, and adult. The antennal discs have a distorted shape and are significantly larger in size than wildtype.

tio^Scer\UAS.cLa under the control of Scer\GAL4^c329b often induces multiple ectopic eyes within a single third-instar antennal imaginal disc, and adult. The antennal discs have a distorted shape and are significantly larger in size than wildtype.

tio^Scer\UAS.cLa under the control of Scer\GAL4^C833 induces ectopic eye formation in the third-instar antennal imaginal disc and adult. The antennal discs have a distorted shape and are significantly larger in size than wildtype.

tio^Scer\UAS.cLa under the control of Scer\GAL4^MJ33a often induces multiple ectopic eyes within a single third-instar antennal imaginal disc, and adult. The antennal discs have a distorted shape and are significantly larger in size than wildtype.

tio^Scer\UAS.cLa under the control of Scer\GAL4^T98 induces ectopic eye formation in the adult but ectopic tissue is not seen in the third-instar antennal imaginal disc. The antennal discs have a distorted shape and are significantly larger in size than wildtype.

(Datta et al., 2009)

Ectopic expression of tio^Scer\UAS.cLa in the epidermis at 25^oC or 29^oC, driven by Scer\GAL4^69B, causes a homeotic labial to T1 segment transformation, which is visible due to the formation of a denticle T1 "beard" in the labial segment. When mutants are maintained at 29^oC, they experience a loss of denticles on the trunk, an effect that is not seen at 25^oC.

(Laugier et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

tio^UAS.cLa/Scer\GAL4^tsh-c724 is a suppressor | partially of partially lethal phenotype of Scer\GAL4^tsh-c724, tsh^JF02856

(Denholm et al., 2013)

tio^UAS.cLa/Scer\GAL4^tsh-c724 is a suppressor of visible phenotype of Scer\GAL4^tsh-c724, tsh^JF02856

(Denholm et al., 2013)

tio^UAS.cLa/Scer\GAL4^69B is a suppressor of visible | homeotic phenotype of tsh⁸

(Laugier et al., 2005)

Phenotype Manifest In

Suppressor of

Statement

Reference

tio^UAS.cLa/Scer\GAL4^tsh-c724 is a suppressor of adult Malpighian tubule Type II cell phenotype of Scer\GAL4^tsh-c724, tsh^JF02856

(Denholm et al., 2013)

tio^UAS.cLa/Scer\GAL4^tsh-c724 is a suppressor of adult abdomen phenotype of Scer\GAL4^tsh-c724, tsh^JF02856

(Denholm et al., 2013)

tio^UAS.cLa/Scer\GAL4^69B is a suppressor of denticle row 3 phenotype of tsh⁸

(Laugier et al., 2005)

tio^UAS.cLa/Scer\GAL4^69B is a suppressor of denticle row 4 phenotype of tsh⁸

(Laugier et al., 2005)

tio^UAS.cLa/Scer\GAL4^69B is a suppressor of prothoracic segment phenotype of tsh⁸

(Laugier et al., 2005)

NOT Suppressor of

Statement

Reference

tio^UAS.cLa/Scer\GAL4^69B is a non-suppressor of denticle row 1 phenotype of tsh⁸

(Laugier et al., 2005)

tio^UAS.cLa/Scer\GAL4^69B is a non-suppressor of denticle row 2 phenotype of tsh⁸

(Laugier et al., 2005)

Other

Statement

Reference

Scer\GAL4^69B, tio^UAS.cLa, tsh⁸ has larval head segment & denticle | ectopic phenotype

(Laugier et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of tio^Scer\UAS.cLa restores stellate cell morphology is restored in adults expressing tsh^JF02856 under the control of Scer\GAL4^tsh-c724. In addition, adult viability is partially rescued (22% of adults eclose) and the adults that emerge do not have bloated abdomens.

(Denholm et al., 2013)

Expression of tio^Scer\UAS.cLa, under the control of Scer\GAL4^69B, partially rescues the denticle row phenotype of tsh⁸ mutants at 25^oC, but fails to rescue this phenotype at 22^oC. At 25^oC, the partially-rescued larvae have five denticle rows, but types 1 and 2 are missing and types 3-6 are not in the same sequential order seen in wild-type larvae. The identity of the T1 segment is restored, shown by the presence of a T1 beard. However, expression of tio^Scer\UAS.cLa causes a new phenotype: ectopic denticles appear in the head region.

(Laugier et al., 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

tio^Scer\UAS.cLa

tio^UAS.cLa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Laugier

(Laugier et al., 2005)

Secondary FlyBase IDs

References (4)

Report Sections

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