Open Close
General Information
Symbol
Dmel\tioUAS.cLa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Laugier
FlyBase ID
FBal0190154
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-tio
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt sequences drive expression of an EcoRI fragment of tio.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

tioScer\UAS.cLa under the control of Scer\GAL4GMR.PF results in roughening of the posterior portion of the adult eye as a result of nonspecific loss of photoreceptor cells within each ommatidium.

tioScer\UAS.cLa under the control of Scer\GAL4dpp.PS induces ectopic eye formation in the third-instar antennal imaginal disc and adult. The antennal discs have a distorted shape and are significantly larger in size than wildtype.

tioScer\UAS.cLa under the control of Scer\GAL4cb26 induces ectopic eye formation in the third-instar antennal imaginal disc and adult. The antennal discs have a distorted shape and are significantly larger in size than wildtype.

tioScer\UAS.cLa under the control of Scer\GAL4cb41 induces ectopic eye formation in the third-instar antennal imaginal disc and adult. The antennal discs have a distorted shape and are significantly larger in size than wildtype.

tioScer\UAS.cLa under the control of Scer\GAL4Ser.PGF often induces multiple ectopic eyes within a single third-instar antennal imaginal disc, and adult. The antennal discs have a distorted shape and are significantly larger in size than wildtype.

tioScer\UAS.cLa under the control of Scer\GAL4c329b often induces multiple ectopic eyes within a single third-instar antennal imaginal disc, and adult. The antennal discs have a distorted shape and are significantly larger in size than wildtype.

tioScer\UAS.cLa under the control of Scer\GAL4C833 induces ectopic eye formation in the third-instar antennal imaginal disc and adult. The antennal discs have a distorted shape and are significantly larger in size than wildtype.

tioScer\UAS.cLa under the control of Scer\GAL4MJ33a often induces multiple ectopic eyes within a single third-instar antennal imaginal disc, and adult. The antennal discs have a distorted shape and are significantly larger in size than wildtype.

tioScer\UAS.cLa under the control of Scer\GAL4T98 induces ectopic eye formation in the adult but ectopic tissue is not seen in the third-instar antennal imaginal disc. The antennal discs have a distorted shape and are significantly larger in size than wildtype.

Ectopic expression of tioScer\UAS.cLa in the epidermis at 25oC or 29oC, driven by Scer\GAL469B, causes a homeotic labial to T1 segment transformation, which is visible due to the formation of a denticle T1 "beard" in the labial segment. When mutants are maintained at 29oC, they experience a loss of denticles on the trunk, an effect that is not seen at 25oC.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressor of
NOT Suppressor of
Statement
Reference

tioUAS.cLa/Scer\GAL469B is a non-suppressor of denticle row 1 phenotype of tsh8

tioUAS.cLa/Scer\GAL469B is a non-suppressor of denticle row 2 phenotype of tsh8

Other
Statement
Reference

Scer\GAL469B, tioUAS.cLa, tsh8 has larval head segment & denticle | ectopic phenotype

Additional Comments
Genetic Interactions
Statement
Reference

Co-expression of tioScer\UAS.cLa restores stellate cell morphology is restored in adults expressing tshJF02856 under the control of Scer\GAL4tsh-c724. In addition, adult viability is partially rescued (22% of adults eclose) and the adults that emerge do not have bloated abdomens.

Expression of tioScer\UAS.cLa, under the control of Scer\GAL469B, partially rescues the denticle row phenotype of tsh8 mutants at 25oC, but fails to rescue this phenotype at 22oC. At 25oC, the partially-rescued larvae have five denticle rows, but types 1 and 2 are missing and types 3-6 are not in the same sequential order seen in wild-type larvae. The identity of the T1 segment is restored, shown by the presence of a T1 beard. However, expression of tioScer\UAS.cLa causes a new phenotype: ectopic denticles appear in the head region.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
tioScer\UAS.cLa
tioUAS.cLa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Laugier
Secondary FlyBase IDs
    References (3)