Allele Dmel\DJ-1α^{dsRNA.Scer\UAS}

General Information
Symbol	Dmel\DJ-1α^{dsRNA.Scer\UAS}	Species	D. melanogaster
Name		FlyBase ID	FBal0190191
Feature type	allele	Associated gene	Dmel\DJ-1α

Allele class
Mutagen	in vitro construct - RNAi
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - RNAi (Yang et al., 2005, Yang, 2005)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS sequences drive expression of genomic DJ-1α sequence fused with an inverted DJ-1α cDNA fragment corresponding to the same region to generate dsRNA. (Yang et al., 2005) Construct: Scer\UAS regulatory sequences drive expression of dsRNA-encoding DJ-1α sequences. (Yang, 2005)
Carried in construct	P{UAS-DJ-1α-dsRNA} (Yang, 2005, Yang et al., 2005, Yang et al., 2008, Venderova et al., 2009)
Cytology
Phenotypic Data
Phenotypic Class
	chemical sensitive, with Scer\GAL4^elav-C155 (Yang et al., 2005) increased cell death, with Scer\GAL4^GMR.PF (Yang et al., 2005) lethal \| larval stage, with Scer\GAL4^Act5C.PU (Yang et al., 2005) lethal \| larval stage, with Scer\GAL4^da.G32 (Yang et al., 2005) visible, with Scer\GAL4^GMR.PF (Venderova et al., 2009)
Phenotype Manifest In
	dopaminergic neuron, with Scer\GAL4^Ddc.PL (Yang et al., 2005) dopaminergic neuron, with Scer\GAL4^elav-C155 (Yang et al., 2005) dopaminergic neuron, with Scer\GAL4^ple.PF (Yang et al., 2005) eye, with Df(2R)CX1, Scer\GAL4^GMR.PF (Yang et al., 2005) eye, with Scer\GAL4^GMR.PF (Yang et al., 2005, Venderova et al., 2009) eye photoreceptor cell, with Scer\GAL4^GMR.PF (Yang et al., 2005) ommatidium, with Scer\GAL4^GMR.PF (Yang et al., 2005)
Detailed Description
	Statement Reference Flies expressing DJ-1α[dsRNA.Scer\UAS] under the control of Scer\GAL4[GMR.PF] at 29[o]C have black lesions on the eye surface. (Venderova et al., 2009) Induction of DJ-1α^{dsRNA.Scer\UAS} in the developing eye using Scer\GAL4^GMR.PF produces a rough eye phenotype, caused through a loss of photoreceptor neurons and some ommatidia. This RNAi effect is dosage dependent as increasing the copy number of Scer\GAL4^GMR.PF and DJ-1α^{dsRNA.Scer\UAS} results in a more severe degeneration of the eye. In a Df(2R)CX1 heterozygous background (Df(2R)CX1 removes DJ-1α), the eye phenotype of DJ-1α^{dsRNA.Scer\UAS}/Scer\GAL4^GMR.PF is significantly enhanced. Expression of DJ-1α^{dsRNA.Scer\UAS}, under the control of Scer\GAL4^Ddc.PL results in an age-dependent reduction in the number of ple-positive (dopaminergic) neurons in the dorsomedial clusters. In 1-day-old flies, a normal complement of ple positive neurons (approximately 18) is present, but, in 35-day and old flies, only 10-12 of these neurons can be detected. Induction of DJ-1α^{dsRNA.Scer\UAS} with Scer\GAL4^ple.PF or Scer\GAL4^elav-C155 results in a reduction in ple-positive dopaminergic neurons in the dorsomedial clusters. In newly eclosed flies the numbers of dopaminergic neurons is comparable between control and DJ-1α^{dsRNA.Scer\UAS} (Scer\GAL4^ple.PF) flies. However, 1 day after eclosion, DJ-1α^{dsRNA.Scer\UAS} flies exhibit significantly reduced dopaminergic neuron levels compared to wild-type. At 4,7 and 10 dyas of age, control flies and DJ-1α^{dsRNA.Scer\UAS} flies exhibit an age-dependent decline in dopaminergic neurons, although DJ-1α^{dsRNA.Scer\UAS} flies consistently exhibit more reduction than in controls. When treated with 1% H₂O₂, the time to reach 50% mortality is reduced by 27% in DJ-1α^{dsRNA.Scer\UAS} (under the control of Scer\GAL4^elav-C155) compared to controls. DJ-1α^{dsRNA.Scer\UAS} (under the control of Scer\GAL4^elav-C155) flies are more sensitive to 3-AT (a known inhibitor of catalase, which converts H₂O₂ to H₂0) exposure compared to control flies. (Yang et al., 2005) Induction of DJ-1α^{dsRNA.Scer\UAS} in aduly fly dopaminergic neurons (through Scer\GAL4^ple.PF leads to an elevation in the levels of reactive oxygen species (ROS). (Yang, 2005)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference DJ-1α^{dsRNA.Scer\UAS}, Scer\GAL4^Ddc.PL has increased cell death phenotype, enhanceable by Pi3K92E^{D954A.Scer\UAS.T:Hsap\MYC}, Scer\GAL4^Ddc.PL (Yang et al., 2005) DJ-1α^{dsRNA.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has increased cell death phenotype, enhanceable by Pi3K92E^{D954A.Scer\UAS.T:Hsap\MYC}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Yang et al., 2005)
Suppressed by
Statement Reference DJ-1α^{dsRNA.Scer\UAS}, Scer\GAL4^Ddc.PL has increased cell death phenotype, suppressible by Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^Ddc.PL (Yang et al., 2005) DJ-1α^{dsRNA.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has increased cell death phenotype, suppressible \| partially by Hsap\PARK7^Scer\UAS.cYa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Yang et al., 2005) DJ-1α^{dsRNA.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has increased cell death phenotype, suppressible by Akt1^{Scer\UAS.T:Ivir\HA1}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Yang et al., 2005) DJ-1α^{dsRNA.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has increased cell death phenotype, suppressible by Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Yang et al., 2005)
Other
Statement Reference DJ-1α^{dsRNA.Scer\UAS}, Pten^Scer\UAS.cHa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has increased cell death phenotype (Yang et al., 2005, Yang et al., 2005)
Phenotype Manifest In
Enhanced by
Statement Reference DJ-1α^{dsRNA.Scer\UAS}, Scer\GAL4^Ddc.PL has dopaminergic neuron phenotype, enhanceable by Pi3K92E^{D954A.Scer\UAS.T:Hsap\MYC}, Scer\GAL4^Ddc.PL (Yang et al., 2005) DJ-1α^{dsRNA.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has eye phenotype, enhanceable by Pi3K92E^{D954A.Scer\UAS.T:Hsap\MYC}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Yang et al., 2005) DJ-1α^{dsRNA.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has eye phenotype, enhanceable by Pten^Scer\UAS.cHa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Yang et al., 2005) DJ-1α^{dsRNA.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has eye photoreceptor cell phenotype, enhanceable by Pi3K92E^{D954A.Scer\UAS.T:Hsap\MYC}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Yang et al., 2005) DJ-1α^{dsRNA.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has ommatidium phenotype, enhanceable by Pi3K92E^{D954A.Scer\UAS.T:Hsap\MYC}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Yang et al., 2005)
Suppressed by
Statement Reference DJ-1α^{dsRNA.Scer\UAS}, Scer\GAL4^Ddc.PL has dopaminergic neuron phenotype, suppressible by Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^Ddc.PL (Yang et al., 2005) DJ-1α^{dsRNA.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has eye phenotype, suppressible \| partially by Hsap\PARK7^Scer\UAS.cYa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Yang et al., 2005) DJ-1α^{dsRNA.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has eye phenotype, suppressible by Akt1^{Scer\UAS.T:Ivir\HA1}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Yang et al., 2005) DJ-1α^{dsRNA.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has eye phenotype, suppressible by Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Yang et al., 2005) DJ-1α^{dsRNA.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has eye photoreceptor cell phenotype, suppressible \| partially by Hsap\PARK7^Scer\UAS.cYa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Yang et al., 2005) DJ-1α^{dsRNA.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has eye photoreceptor cell phenotype, suppressible by Akt1^{Scer\UAS.T:Ivir\HA1}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Yang et al., 2005) DJ-1α^{dsRNA.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has eye photoreceptor cell phenotype, suppressible by Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Yang et al., 2005) DJ-1α^{dsRNA.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has ommatidium phenotype, suppressible \| partially by Hsap\PARK7^Scer\UAS.cYa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Yang et al., 2005) DJ-1α^{dsRNA.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has ommatidium phenotype, suppressible by Akt1^{Scer\UAS.T:Ivir\HA1}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Yang et al., 2005) DJ-1α^{dsRNA.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has ommatidium phenotype, suppressible by Pi3K92E^{Scer\UAS.T:Hsap\MYC}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Yang et al., 2005)
NOT suppressed by
Statement Reference DJ-1α^{dsRNA.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has eye phenotype, non-suppressible by Avic\GFP^Scer\UAS.cUa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Yang et al., 2005) DJ-1α^{dsRNA.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has eye photoreceptor cell phenotype, non-suppressible by Avic\GFP^Scer\UAS.cUa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Yang et al., 2005) DJ-1α^{dsRNA.Scer\UAS}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has ommatidium phenotype, non-suppressible by Avic\GFP^Scer\UAS.cUa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Yang et al., 2005)
Other
Statement Reference DJ-1α^{dsRNA.Scer\UAS}, Pten^Scer\UAS.cHa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF has ommatidium phenotype (Yang et al., 2005, Yang et al., 2005)
Additional Comments
Genetic Interactions
Statement Reference Coexpression of Pten^Scer\UAS.cHa with DJ-1α^{dsRNA.Scer\UAS}, under the control of Scer\GAL4^GMR.PF enhances the DJ-1α^{dsRNA.Scer\UAS}-induced eye phenotype. The resulting eyes are dramatically reduced in size, with collapsed and fused ommatidia and necrotic spots, which are not present in DJ-1α^{dsRNA.Scer\UAS} flies. There is also a near complete loss of photoreceptor neurons. Coexpression of Pi3K92E^{D954A.Scer\UAS.T:Hsap\MYC} with DJ-1α^{dsRNA.Scer\UAS}, under the control of Scer\GAL4^GMR.PF enhances the DJ-1α^{dsRNA.Scer\UAS}-induced eye phenotype. Coexpression of Pi3K92E^{D954A.Scer\UAS.T:Hsap\MYC} with DJ-1α^{dsRNA.Scer\UAS}, under the control of Scer\GAL4^Ddc.PL results in a statistically significant enhancement of the dopaminergic (DA) degeneration phenotype of DJ-1α^{dsRNA.Scer\UAS} mutants. Coexpression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} with DJ-1α^{dsRNA.Scer\UAS}, under the control of Scer\GAL4^GMR.PF suppresses the DJ-1α^{dsRNA.Scer\UAS}-induced eye phenotype. The eyes are restored to normal size, and the organisation of the ommatidia is significantly improved. Coexpression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} with DJ-1α^{dsRNA.Scer\UAS}, under the control of Scer\GAL4^Ddc.PL completely suppresses the dopaminergic (DA) degeneration phenotype of DJ-1α^{dsRNA.Scer\UAS} mutants. The number of DA neurons remains consistent throughout. Coexpression of Akt1^{Scer\UAS.T:Ivir\HA1} with DJ-1α^{dsRNA.Scer\UAS}, under the control of Scer\GAL4^GMR.PF suppresses the DJ-1α^{dsRNA.Scer\UAS}-induced eye phenotype. (Yang et al., 2005) DJ-1α^{dsRNA.Scer\UAS} flies coexpressing Pi3K92E^{Scer\UAS.T:Hsap\MYC} (both in the dopaminergic neurons through Scer\GAL4^ple.PF) exhibit basal levels of reactive oxygen species (ROS), as in wild-type. DJ-1α^{dsRNA.Scer\UAS} flies coexpressing Pten^Scer\UAS.cHa (both in the dopaminergic neurons through Scer\GAL4^ple.PF) exhibit higher levels of reactive oxygen species (ROS), compared to flies expressing DJ-1α^{dsRNA.Scer\UAS} alone. (Yang, 2005)
Xenogenetic Interactions
Statement Reference Co-expression of Hsap\DJ-1A^Scer\UAS.cYa with DJ-1α^{dsRNA.Scer\UAS}, under the regulation of Scer\GAL4^GMR.PF partially suppresses the eye degeneration phenotype observed in DJ-1α^{dsRNA.Scer\UAS} (Scer\GAL4^GMR.PF) mutants. Co-expression of Avic\GFP^Scer\UAS with DJ-1α^{dsRNA.Scer\UAS}, under the regulation of Scer\GAL4^GMR.PF has no effect on the DJ-1α^{dsRNA.Scer\UAS} eye degeneration phenotype. (Yang et al., 2005)
Complementation & Rescue Data
Partially rescued by	DJ-1α^{dsRNA.Scer\UAS} is partially rescued by DJ-1α^Scer\UAS.cYa/Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Yang et al., 2005)
Comments	Expression of a DJ-1α^Scer\UAS.cYa transgene partially rescues the DJ-1α^{dsRNA.Scer\UAS} (Scer\GAL4^GMR.PF) eye degeneration phenotype. (Yang et al., 2005)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	DJ-1α^{dsRNA.Scer\UAS}
Name Synonym
Secondary FlyBase IDs

References ( 4 )
Research paper	Venderova et al., 2009, Hum. Mol. Genet. 18(22): 4390--4404 Leucine-Rich Repeat Kinase 2 interacts with Parkin, DJ-1 and PINK-1 in a Drosophila melanogaster model of Parkinson's disease. [FBrf0209058] Yang et al., 2008, Proc. Natl. Acad. Sci. U.S.A. 105(19): 7070--7075 Pink1 regulates mitochondrial dynamics through interaction with the fission/fusion machinery. [FBrf0205047] Yang et al., 2005, Proc. Natl. Acad. Sci. U.S.A. 102(38): 13670--13675 Inactivation of Drosophila DJ-1 leads to impairments of oxidative stress response and phosphatidylinositol 3-kinase/Akt signaling. [FBrf0188284]
Supplementary material	Yang, 2005, Proc. Natl. Acad. Sci. USA 102(38): [title not yet available] [FBrf0191581]

Allele Dmel\DJ-1αdsRNA.Scer\UAS

Allele Dmel\DJ-1α^{dsRNA.Scer\UAS}