FlyBase Allele Report: Dmel\DJ-1α[RNAi.UAS]

General Information

Symbol

Dmel\DJ-1α^RNAi.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0190191

Feature type

allele

Associated gene

Dmel\DJ-1α

Associated Insertion(s)

Carried in Construct

P{UAS-DJ-1α-RNAi}

Also Known As

DJ-1A RNAi

Key Links

Transgenic product class

RNAi_reagent

(Yang et al., 2005)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Yang et al., 2005)

Mutagen

in vitro construct

(Yang, 2005, Yang et al., 2005)

Progenitor genotype

Carried in construct

P{UAS-DJ-1α-RNAi}

Cytology

Description

UAS sequences drive expression of genomic DJ-1α sequence fused with an inverted DJ-1α cDNA fragment corresponding to the same region to generate dsRNA.

(Yang et al., 2005)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

DJ-1α

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Parkinson's disease

CEA

(Faust et al., 2009, Yang et al., 2005)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of Parkinson's disease

is exacerbated by Pi3K92E^{D954A.UAS.Tag:MYC}

(Yang et al., 2005)

is ameliorated by Pi3K92E^UAS.Tag:MYC

(Yang et al., 2005)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

dopaminergic neuron, with Scer\GAL4^Ddc.PL

(Yang et al., 2005)

dopaminergic neuron, with Scer\GAL4^elav-C155

(Yang et al., 2005)

dopaminergic neuron, with Scer\GAL4^ple.PF

(Yang et al., 2005)

dopaminergic neuron | adult stage | progressive, with Scer\GAL4^Ddc.PL

(Faust et al., 2009)

eye, with Df(2R)CX1, Scer\GAL4^GMR.PF

(Yang et al., 2005)

eye, with Scer\GAL4^GMR.PF

(Venderova et al., 2009, Yang et al., 2005)

eye photoreceptor cell, with Scer\GAL4^GMR.PF

(Yang et al., 2005)

ommatidium, with Scer\GAL4^GMR.PF

(Yang et al., 2005)

Detailed Description

Statement

Reference

Expression of DJ-1α^{dsRNA.Scer\UAS} under the control of Scer\GAL4^Ddc.PL results in accelerated age-dependent loss of dopaminergic neurons in adult flies (reared on a yeast-paste diet) aged flies also display decreased climbing ability relative to controls.

(Faust et al., 2009)

Flies expressing DJ-1α^{dsRNA.Scer\UAS} under the control of Scer\GAL4^GMR.PF at 29[o]C have black lesions on the eye surface.

(Venderova et al., 2009)

Induction of DJ-1α^{dsRNA.Scer\UAS} in the developing eye using Scer\GAL4^GMR.PF produces a rough eye phenotype, caused through a loss of photoreceptor neurons and some ommatidia. This RNAi effect is dosage dependent as increasing the copy number of Scer\GAL4^GMR.PF and DJ-1α^{dsRNA.Scer\UAS} results in a more severe degeneration of the eye. In a Df(2R)CX1 heterozygous background (Df(2R)CX1 removes DJ-1α), the eye phenotype of DJ-1α^{dsRNA.Scer\UAS}/Scer\GAL4^GMR.PF is significantly enhanced. Expression of DJ-1α^{dsRNA.Scer\UAS}, under the control of Scer\GAL4^Ddc.PL results in an age-dependent reduction in the number of ple-positive (dopaminergic) neurons in the dorsomedial clusters. In 1-day-old flies, a normal complement of ple positive neurons (approximately 18) is present, but, in 35-day and old flies, only 10-12 of these neurons can be detected. Induction of DJ-1α^{dsRNA.Scer\UAS} with Scer\GAL4^ple.PF or Scer\GAL4^elav-C155 results in a reduction in ple-positive dopaminergic neurons in the dorsomedial clusters. In newly eclosed flies the numbers of dopaminergic neurons is comparable between control and DJ-1α^{dsRNA.Scer\UAS} (Scer\GAL4^ple.PF) flies. However, 1 day after eclosion, DJ-1α^{dsRNA.Scer\UAS} flies exhibit significantly reduced dopaminergic neuron levels compared to wild-type. At 4,7 and 10 dyas of age, control flies and DJ-1α^{dsRNA.Scer\UAS} flies exhibit an age-dependent decline in dopaminergic neurons, although DJ-1α^{dsRNA.Scer\UAS} flies consistently exhibit more reduction than in controls. When treated with 1% H₂O₂, the time to reach 50% mortality is reduced by 27% in DJ-1α^{dsRNA.Scer\UAS} (under the control of Scer\GAL4^elav-C155) compared to controls. DJ-1α^{dsRNA.Scer\UAS} (under the control of Scer\GAL4^elav-C155) flies are more sensitive to 3-AT (a known inhibitor of catalase, which converts H₂O₂ to H₂0) exposure compared to control flies.

(Yang et al., 2005)

Induction of DJ-1α^{dsRNA.Scer\UAS} in aduly fly dopaminergic neurons (through Scer\GAL4^ple.PF leads to an elevation in the levels of reactive oxygen species (ROS).

(Yang, 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

DJ-1α^RNAi.UAS, Scer\GAL4^Ddc.PL has increased cell death phenotype, enhanceable by Pi3K92E^{D954A.UAS.Tag:MYC}, Scer\GAL4^Ddc.PL

(Yang et al., 2005)

DJ-1α^RNAi.UAS, Scer\GAL4^GMR.PF has increased cell death phenotype, enhanceable by Pi3K92E^{D954A.UAS.Tag:MYC}, Scer\GAL4^GMR.PF

(Yang et al., 2005)

Suppressed by

Statement

Reference

DJ-1α^RNAi.UAS, Scer\GAL4^Ddc.PL has abnormal neuroanatomy | adult stage | progressive phenotype, suppressible by Hsap\PARK7^UAS.cYa, Scer\GAL4^Ddc.PL

(Faust et al., 2009)

DJ-1α^RNAi.UAS, Scer\GAL4^Ddc.PL has abnormal locomotor behavior | adult stage phenotype, suppressible by Hsap\PARK7^UAS.cYa, Scer\GAL4^Ddc.PL

(Faust et al., 2009)

DJ-1α^RNAi.UAS, Scer\GAL4^GMR.PF has increased cell death phenotype, suppressible by Akt^UAS.Tag:HA, Scer\GAL4^GMR.PF

(Yang et al., 2005)

DJ-1α^RNAi.UAS, Scer\GAL4^Ddc.PL has increased cell death phenotype, suppressible by Pi3K92E^UAS.Tag:MYC, Scer\GAL4^Ddc.PL

(Yang et al., 2005)

DJ-1α^RNAi.UAS, Scer\GAL4^GMR.PF has increased cell death phenotype, suppressible by Pi3K92E^UAS.Tag:MYC, Scer\GAL4^GMR.PF

(Yang et al., 2005)

DJ-1α^RNAi.UAS, Scer\GAL4^GMR.PF has increased cell death phenotype, suppressible | partially by Hsap\PARK7^UAS.cYa, Scer\GAL4^GMR.PF

(Yang et al., 2005)

Other

Statement

Reference

DJ-1α^RNAi.UAS, Pten^UAS.cHa, Scer\GAL4^GMR.PF has increased cell death phenotype

(Yang et al., 2005)

Phenotype Manifest In

Enhanced by

Statement

Reference

DJ-1α^RNAi.UAS, Scer\GAL4^Ddc.PL has dopaminergic neuron phenotype, enhanceable by Pi3K92E^{D954A.UAS.Tag:MYC}, Scer\GAL4^Ddc.PL

(Yang et al., 2005)

DJ-1α^RNAi.UAS, Scer\GAL4^GMR.PF has eye photoreceptor cell phenotype, enhanceable by Pi3K92E^{D954A.UAS.Tag:MYC}, Scer\GAL4^GMR.PF

(Yang et al., 2005)

DJ-1α^RNAi.UAS, Scer\GAL4^GMR.PF has eye phenotype, enhanceable by Pi3K92E^{D954A.UAS.Tag:MYC}, Scer\GAL4^GMR.PF

(Yang et al., 2005)

DJ-1α^RNAi.UAS, Scer\GAL4^GMR.PF has ommatidium phenotype, enhanceable by Pi3K92E^{D954A.UAS.Tag:MYC}, Scer\GAL4^GMR.PF

(Yang et al., 2005)

DJ-1α^RNAi.UAS, Scer\GAL4^GMR.PF has eye phenotype, enhanceable by Pten^UAS.cHa, Scer\GAL4^GMR.PF

(Yang et al., 2005)

Suppressed by

Statement

Reference

DJ-1α^RNAi.UAS, Scer\GAL4^Ddc.PL has dopaminergic neuron | adult stage | progressive phenotype, suppressible by Hsap\PARK7^UAS.cYa, Scer\GAL4^Ddc.PL

(Faust et al., 2009)

DJ-1α^RNAi.UAS, Scer\GAL4^Ddc.PL has dopaminergic neuron phenotype, suppressible by Pi3K92E^UAS.Tag:MYC, Scer\GAL4^Ddc.PL

(Yang et al., 2005)

DJ-1α^RNAi.UAS, Scer\GAL4^GMR.PF has eye photoreceptor cell phenotype, suppressible by Akt^UAS.Tag:HA, Scer\GAL4^GMR.PF

(Yang et al., 2005)

DJ-1α^RNAi.UAS, Scer\GAL4^GMR.PF has eye photoreceptor cell phenotype, suppressible by Pi3K92E^UAS.Tag:MYC, Scer\GAL4^GMR.PF

(Yang et al., 2005)

DJ-1α^RNAi.UAS, Scer\GAL4^GMR.PF has eye phenotype, suppressible by Akt^UAS.Tag:HA, Scer\GAL4^GMR.PF

(Yang et al., 2005)

DJ-1α^RNAi.UAS, Scer\GAL4^GMR.PF has eye phenotype, suppressible by Pi3K92E^UAS.Tag:MYC, Scer\GAL4^GMR.PF

(Yang et al., 2005)

DJ-1α^RNAi.UAS, Scer\GAL4^GMR.PF has ommatidium phenotype, suppressible by Akt^UAS.Tag:HA, Scer\GAL4^GMR.PF

(Yang et al., 2005)

DJ-1α^RNAi.UAS, Scer\GAL4^GMR.PF has ommatidium phenotype, suppressible by Pi3K92E^UAS.Tag:MYC, Scer\GAL4^GMR.PF

(Yang et al., 2005)

DJ-1α^RNAi.UAS, Scer\GAL4^GMR.PF has eye photoreceptor cell phenotype, suppressible | partially by Hsap\PARK7^UAS.cYa, Scer\GAL4^GMR.PF

(Yang et al., 2005)

DJ-1α^RNAi.UAS, Scer\GAL4^GMR.PF has eye phenotype, suppressible | partially by Hsap\PARK7^UAS.cYa, Scer\GAL4^GMR.PF

(Yang et al., 2005)

DJ-1α^RNAi.UAS, Scer\GAL4^GMR.PF has ommatidium phenotype, suppressible | partially by Hsap\PARK7^UAS.cYa, Scer\GAL4^GMR.PF

(Yang et al., 2005)

NOT suppressed by

Statement

Reference

DJ-1α^RNAi.UAS, Scer\GAL4^GMR.PF has eye photoreceptor cell phenotype, non-suppressible by Avic\GFP^UAS.cUa, Scer\GAL4^GMR.PF

(Yang et al., 2005)

DJ-1α^RNAi.UAS, Scer\GAL4^GMR.PF has eye phenotype, non-suppressible by Avic\GFP^UAS.cUa, Scer\GAL4^GMR.PF

(Yang et al., 2005)

DJ-1α^RNAi.UAS, Scer\GAL4^GMR.PF has ommatidium phenotype, non-suppressible by Avic\GFP^UAS.cUa, Scer\GAL4^GMR.PF

(Yang et al., 2005)

Other

Statement

Reference

DJ-1α^RNAi.UAS, Pten^UAS.cHa, Scer\GAL4^GMR.PF has ommatidium phenotype

(Yang et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

Coexpression of Pten^Scer\UAS.cHa with DJ-1α^{dsRNA.Scer\UAS}, under the control of Scer\GAL4^GMR.PF enhances the DJ-1α^{dsRNA.Scer\UAS}-induced eye phenotype. The resulting eyes are dramatically reduced in size, with collapsed and fused ommatidia and necrotic spots, which are not present in DJ-1α^{dsRNA.Scer\UAS} flies. There is also a near complete loss of photoreceptor neurons. Coexpression of Pi3K92E^{D954A.Scer\UAS.T:Hsap\MYC} with DJ-1α^{dsRNA.Scer\UAS}, under the control of Scer\GAL4^GMR.PF enhances the DJ-1α^{dsRNA.Scer\UAS}-induced eye phenotype. Coexpression of Pi3K92E^{D954A.Scer\UAS.T:Hsap\MYC} with DJ-1α^{dsRNA.Scer\UAS}, under the control of Scer\GAL4^Ddc.PL results in a statistically significant enhancement of the dopaminergic (DA) degeneration phenotype of DJ-1α^{dsRNA.Scer\UAS} mutants. Coexpression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} with DJ-1α^{dsRNA.Scer\UAS}, under the control of Scer\GAL4^GMR.PF suppresses the DJ-1α^{dsRNA.Scer\UAS}-induced eye phenotype. The eyes are restored to normal size, and the organisation of the ommatidia is significantly improved. Coexpression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} with DJ-1α^{dsRNA.Scer\UAS}, under the control of Scer\GAL4^Ddc.PL completely suppresses the dopaminergic (DA) degeneration phenotype of DJ-1α^{dsRNA.Scer\UAS} mutants. The number of DA neurons remains consistent throughout. Coexpression of Akt1^{Scer\UAS.T:Ivir\HA1} with DJ-1α^{dsRNA.Scer\UAS}, under the control of Scer\GAL4^GMR.PF suppresses the DJ-1α^{dsRNA.Scer\UAS}-induced eye phenotype.

(Yang et al., 2005)

DJ-1α^{dsRNA.Scer\UAS} flies coexpressing Pi3K92E^{Scer\UAS.T:Hsap\MYC} (both in the dopaminergic neurons through Scer\GAL4^ple.PF) exhibit basal levels of reactive oxygen species (ROS), as in wild-type. DJ-1α^{dsRNA.Scer\UAS} flies coexpressing Pten^Scer\UAS.cHa (both in the dopaminergic neurons through Scer\GAL4^ple.PF) exhibit higher levels of reactive oxygen species (ROS), compared to flies expressing DJ-1α^{dsRNA.Scer\UAS} alone.

(Yang, 2005)

Xenogenetic Interactions

Statement

Reference

The accelerated age-dependent loss of dopaminergic neurons as well as the decrease in climbing ability in aged adults characteristic for flies expressing DJ-1α^{dsRNA.Scer\UAS} under the control of Scer\GAL4^Ddc.PL (reared on a yeast-paste diet) is rescued by co-expression of Hsap\PARK7^Scer\UAS.cYa.

(Faust et al., 2009)

Co-expression of Hsap\DJ-1A^Scer\UAS.cYa with DJ-1α^{dsRNA.Scer\UAS}, under the regulation of Scer\GAL4^GMR.PF partially suppresses the eye degeneration phenotype observed in DJ-1α^{dsRNA.Scer\UAS} (Scer\GAL4^GMR.PF) mutants. Co-expression of Avic\GFP^Scer\UAS with DJ-1α^{dsRNA.Scer\UAS}, under the regulation of Scer\GAL4^GMR.PF has no effect on the DJ-1α^{dsRNA.Scer\UAS} eye degeneration phenotype.

(Yang et al., 2005)

Complementation and Rescue Data

Partially rescued by

DJ-1α^RNAi.UAS is partially rescued by DJ-1α^UAS.cYa/Scer\GAL4^GMR.PF

(Yang et al., 2005)

Comments

Expression of a DJ-1α^Scer\UAS.cYa transgene partially rescues the DJ-1α^{dsRNA.Scer\UAS} (Scer\GAL4^GMR.PF) eye degeneration phenotype.

(Yang et al., 2005)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

DJ-1α^RNAi.UAS

DJ-1α^{dsRNA.Scer\UAS}

DJ-1α^dsRNA.UAS

Name Synonyms

Secondary FlyBase IDs

References (7)

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