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General Information
Symbol
Dmel\cv-cC524
Species
D. melanogaster
Name
FlyBase ID
FBal0190240
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:

C14395005T

Amino acid change:

R369term | cv-c-PA; R1703term | cv-c-PC; R1703term | cv-c-PD

Reported amino acid change:

R369term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Mutation results in a truncation of the protein before the GAP domain.

Amino acid replacement: R369term.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

cv-cC524/cv-cMB03717 transheterozygous adults reared under 12h light:12h dark cycles exhibit sleep defects, as shown by significant decreases in sleep time and in sleep bout during both light and dark periods, a significant increase in the latency time to sleep from the onset of darkness, and a significantly lower sleep recovery after overnight sleep deprivation, but do not exhibit circadian rhythm defects, as shown by the insignificant difference in mean circadian locomotor period (and the absence of arrhythmic individuals) in permanent dark conditions after training in 12h light:12h dark cycles, and do not exhibit signs of hyperactivity, as shown by the insignificant differences in the number of walking events and in the arousal threshold by vibrational force, as compared to heterozygous and wild-type controls. These mutant adults exhibit a significant decrease in short-term olfactory memory, assessed by aversive tests to the 4-methylcyclohexanol odorant after electric shock-coupled training, under ad libitum sleep, but not sleep deprivation, conditions, but do not exhibit significant differences in electric shock sensitivity, assessed by maximal motor velocities, nor significant differences in the preference score of naive individuals to the 4-methylcyclohexanol odorant, under ad libitum sleep or sleep deprivation conditions, as compared to heterozygous and wild-type controls; additional expression of cv-cScer\UAS.cDa under the control of Scer\GAL4GMR23E10 in these heterozygotes does not result in significant changes in the sensitivity to electric shocks, as assessed by maximal motor velocities, or in the preference score to the 4-methylcyclohexanol odorant in ad libitum sleep conditions, as compared to controls.

The dorsal fan-shaped neurons of cv-cC524/cv-cMB03717 transheterozygous adults exhibit electrophysiological defects, as shown by the significant decreases in input resistance, membrane time constant and spiking activity at ad libitum sleep conditions, and by the failure to significantly increase the input resistance, the membrane time constant and the spiking activity in response to overnight sleep deprivation, but exhibit no obvious defects in morphology or enervation pattern, as compared to controls. Neurotransmission in olfactory projection neurons of cv-cC524/cv-cMB03717 transheterozygous adults, however, does not display significant changes in input resistance and membrane time constant at ad libitum sleep conditions, as compared to controls.

cv-cC524/cv-cMB01956 and cv-cC524/cv-cDG20401 transheterozygous adults reared under 12h light:12h dark cycles exhibit a significant decrease in sleep time, as compared to heterozygous and wild-type controls.

The amplitudes of excitatory junction potentials (EJPs) evoked by stimulation at 0.3 Hz and recorded at third instar larval neuromuscular junctions of heterozygous cv-cC524/+ and cv-c1/cv-cC524 mutants are significantly increased above control levels. As a result, quantal content (QC) is about 70% higher than the QC in the wild-type control.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescued by
Comments

cv-cC524/cv-cMB03717 transheterozygous adults reared under 12h light:12h dark cycles present a decreased mean sleep time, which is fully rescued by the expression of cv-cScer\UAS.cDa under the control of either Scer\GAL4104Y or Scer\GAL4GMR23E10; cv-cC524/cv-cMB03717 transheterozygous adults also present a lower sleep recovery after overnight sleep deprivation and a short-term olfactory memory decrease under ad libitum sleep conditions, which are also rescued by the expression of cv-cScer\UAS.cDa under the control of Scer\GAL4GMR23E10.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
    References (3)