Mutation results in a truncation of the protein before the GAP domain.
Amino acid replacement: R369term.
C14395005T
R369term | cv-c-PA; R1703term | cv-c-PC; R1703term | cv-c-PD
R369term
Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.
cv-cC524/cv-cMB03717 transheterozygous adults reared under 12h light:12h dark cycles exhibit sleep defects, as shown by significant decreases in sleep time and in sleep bout during both light and dark periods, a significant increase in the latency time to sleep from the onset of darkness, and a significantly lower sleep recovery after overnight sleep deprivation, but do not exhibit circadian rhythm defects, as shown by the insignificant difference in mean circadian locomotor period (and the absence of arrhythmic individuals) in permanent dark conditions after training in 12h light:12h dark cycles, and do not exhibit signs of hyperactivity, as shown by the insignificant differences in the number of walking events and in the arousal threshold by vibrational force, as compared to heterozygous and wild-type controls. These mutant adults exhibit a significant decrease in short-term olfactory memory, assessed by aversive tests to the 4-methylcyclohexanol odorant after electric shock-coupled training, under ad libitum sleep, but not sleep deprivation, conditions, but do not exhibit significant differences in electric shock sensitivity, assessed by maximal motor velocities, nor significant differences in the preference score of naive individuals to the 4-methylcyclohexanol odorant, under ad libitum sleep or sleep deprivation conditions, as compared to heterozygous and wild-type controls; additional expression of cv-cScer\UAS.cDa under the control of Scer\GAL4GMR23E10 in these heterozygotes does not result in significant changes in the sensitivity to electric shocks, as assessed by maximal motor velocities, or in the preference score to the 4-methylcyclohexanol odorant in ad libitum sleep conditions, as compared to controls.
The dorsal fan-shaped neurons of cv-cC524/cv-cMB03717 transheterozygous adults exhibit electrophysiological defects, as shown by the significant decreases in input resistance, membrane time constant and spiking activity at ad libitum sleep conditions, and by the failure to significantly increase the input resistance, the membrane time constant and the spiking activity in response to overnight sleep deprivation, but exhibit no obvious defects in morphology or enervation pattern, as compared to controls. Neurotransmission in olfactory projection neurons of cv-cC524/cv-cMB03717 transheterozygous adults, however, does not display significant changes in input resistance and membrane time constant at ad libitum sleep conditions, as compared to controls.
cv-cC524/cv-cMB01956 and cv-cC524/cv-cDG20401 transheterozygous adults reared under 12h light:12h dark cycles exhibit a significant decrease in sleep time, as compared to heterozygous and wild-type controls.
The amplitudes of excitatory junction potentials (EJPs) evoked by stimulation at 0.3 Hz and recorded at third instar larval neuromuscular junctions of heterozygous cv-cC524/+ and cv-c1/cv-cC524 mutants are significantly increased above control levels. As a result, quantal content (QC) is about 70% higher than the QC in the wild-type control.
cv-cC524/cv-cMB03717 is rescued by Scer\GAL4104Y/cv-cUAS.cDa
cv-cC524/cv-cMB03717 is partially rescued by Scer\GAL4GMR23E10/cv-cUAS.cDa
cv-cC524/cv-cMB03717 transheterozygous adults reared under 12h light:12h dark cycles present a decreased mean sleep time, which is fully rescued by the expression of cv-cScer\UAS.cDa under the control of either Scer\GAL4104Y or Scer\GAL4GMR23E10; cv-cC524/cv-cMB03717 transheterozygous adults also present a lower sleep recovery after overnight sleep deprivation and a short-term olfactory memory decrease under ad libitum sleep conditions, which are also rescued by the expression of cv-cScer\UAS.cDa under the control of Scer\GAL4GMR23E10.
