FlyBase Allele Report: Dmel\Dronc[I29]

Dronc^I29/Dronc^L32 transheterozygotes exhibit a significant increase in the proportion of hyperplastic testes as compared to controls; these testes exhibit a significant decrease in necrosis at their apical tip (as shown by the number of spermatogonial cysts bearing Propidium Iodine-positive or TUNEL-positive cells), as compared to controls. During spermatogenesis, cystic bulges and waste bags are largely absent and actin investment cones in the individualization complex are uncoordinated, as compared to controls.

(Napoletano et al., 2017)

Homozygous Nc^I29 escapers are unhealthy and do not survive long after eclosion. The programmed cell death of bursCCAP neurons seen in the adult ventral nerve cord of wild type flies after eclosion is partially suppressed. Highly variable numbers of bursCCAP neurons are seen at 3-5 days.

(Lee et al., 2013)

Nc^I24/Nc^I29 mutant males exhibit ~40% less spermatogonial cyst death compared to controls.

Nc^I29/Nc^L32 mutant males exhibit ~60% less spermatogonial cyst death compared to controls.

(Yacobi-Sharon et al., 2013)

Adult eyes mosaic for Nc^I29 are predominantly white, indicating that Nc^I29 mutant cells (that are white) proliferate faster than wild-type cells (that are red) and out-compete them.

Nc^I29 mutant eye clones are significantly larger than their wild-type twin spots.

Nc^I29 mutant eye clones induce ectopic cell proliferation posterior to the second mitotic wave in the eye-antennal disc.

Nc^I29 mutant eye clones exhibit extra ommatidial pigment cells in the pupal retina.

(Verghese et al., 2012)

Nc^I29 homozygotes and Nc^I29/Nc⁵¹ transheterozygotes exhibit a delay in vCrz neuron apoptosis. At 7 hours after puparium formation 75% vCrz neurons are still present although these all die by 48 hours after puparium formation.

(Lee et al., 2011)

Ovaries containing homozygous germline clones have slight defects in programmed cell death in mid-oogenesis, with some egg chambers having condensed nurse cell nuclei but no somatic follicle cells. 10% of stage 14 egg chambers contain persisting nurse cell nuclei.

(Baum et al., 2007)

Salivary glands are degraded by 24 hours after puparium formation (as occurs in wild-type controls) in Nc^I24/Nc^I29 mutants that develop normally.

(Berry and Baehrecke, 2007)

100% of Nc^I29/Nc^I24 male escapers have a rotated genitalia defect. 100% of Nc^I29/Nc^I24 adult escapers have a ballooned wing phenotype.

(Krieser et al., 2007)

Nc^I29 mutants display severe defects during the spermatid individualization process. The cystic bulges are frequently reduced in size or appear flat due to a failure in the appropriate collection of the cytoplasm of the spermatids . The retained cytoplasm is clearly visualized as a trail along the entire length of what was supposed to have been the post-individualized portion of the spermatids. Frequently a large portion of the spermatid cytoplasm is retained in a 'mini' cystic bulge structure, which often contains part of the individualization complex. Waste bags are also reduced in size.

(Arama et al., 2006)

Nc⁵¹/Nc^I29 mutants exhibit a delay in programmed cell death in Crz-expressing neurons in the ventral nerve cord during pupation.

(Choi et al., 2006)

Homozygous clones in the eye result in an excess of interommatidial cells in the retina at 42 hours after puparium formation compared to wild type. Mutant clones at the edge of the eye have extra perimeter ommatidial cells that are never eliminated.

(Mendes et al., 2006)

Homozygous escapers have wings that are less transparent than normal and are curved. Homozygous embryos derived from females carrying homozygous germline clones have a head defect. These embryos show a substantial reduction in the number of apoptotic cells compared to wild type. Stage 17 embryos contain additional midline glia cells compared to wild type. There is a general enlargement of the central nervous system in these embryos. Each chordotonal organ cluster contains on average about 3 additional neurons.

(Xu et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Dronc^I29 has abnormal cell death phenotype, enhanceable by Dcp-1^Prev1

(Lee et al., 2011)

Enhancer of

Statement

Reference

Dronc^I29 is an enhancer of abnormal cell death phenotype of Dcp-1^Prev1

(Lee et al., 2011)

Suppressor of

Statement

Reference

Dronc^I29 is a suppressor of visible | adult stage phenotype of hid^GMR.PG

(Neukomm et al., 2017)

Dronc^I29 is a suppressor of increased cell death | somatic clone | third instar larval stage phenotype of Vps4^3B1

(Legent et al., 2015)

Dronc^I29/Dronc^I29 is a suppressor of increased cell death | third instar larval stage phenotype of Ipk2^20B

(Seeds et al., 2015)

Dronc[+]/Dronc^I29 is a suppressor of visible phenotype of Scer\GAL4^GMR.PU, Srrm1^GE25979

(Fan et al., 2014)

Dronc^I29 is a suppressor of increased cell death phenotype of Scer\GAL4^ey.PU, Ubr3^KK100920

(Huang et al., 2014)

Dronc^I29 is a suppressor | somatic clone of increased cell death | third instar larval stage phenotype of Scer\GAL4^GMR.PU, p53^GUS.PB

(Fan et al., 2010)

Dronc^I29/Dronc^I29 is a suppressor of visible phenotype of Diap1^RNAi.UAS.cLa, Scer\GAL4^GMR.PF

(Leulier et al., 2006)

Dronc^I29/Dronc^I29 is a suppressor of visible | somatic clone phenotype of hid^GMR.PG

(Xu et al., 2005)

NOT Suppressor of

Statement

Reference

Dronc^I29/Dronc^I29 is a non-suppressor of abnormal cell number | somatic clone | spermatogenesis phenotype of Rbf^GL01293, Scer\GAL4^Tub.PU

(Sainz de la Maza et al., 2022)

Dronc^I29/Dronc^I29 is a non-suppressor of visible | adult stage phenotype of Ire1^UAS.Tag:V5, Scer\GAL4^GMR.long

(Yan et al., 2019)

Dronc^I29 is a non-suppressor of FlyBase_internaltransgene_features:cell lethal:cell lethal | somatic clone phenotype of Df(1)su(s)R194, RpL36^+t4

(Kale et al., 2015)

Dronc^I29 is a non-suppressor | somatic clone of decreased cell number | third instar larval stage phenotype of Scer\GAL4^GMR.PU, p53^GUS.PB

(Fan et al., 2010)

Other

Statement

Reference

Dronc^I29, Strica⁴ has female fertile | germline clone phenotype

(Baum et al., 2007)

Phenotype Manifest In

Enhanced by

Statement

Reference

Dronc^I29 has neuron phenotype, enhanceable by Dcp-1^Prev1

(Lee et al., 2011)

Enhancer of

Statement

Reference

Dronc^I29 is an enhancer of neuron phenotype of Dcp-1^Prev1

(Lee et al., 2011)

Dronc^I24/Dronc^I29 is an enhancer of embryonic/larval salivary gland phenotype of Pi3K92E^UAS.Tag:MYC, Scer\GAL4^fkh.PH

(Berry and Baehrecke, 2007)

Suppressor of

Statement

Reference

Dronc^I29 is a suppressor of eye phenotype of hid^GMR.PG

(Neukomm et al., 2017)

Dronc^I29 is a suppressor of eye disc | somatic clone | third instar larval stage phenotype of Vps4^3B1

(Legent et al., 2015)

Dronc^I29 is a suppressor of photoreceptor cell R8 | somatic clone | third instar larval stage phenotype of Vps4^3B1

(Legent et al., 2015)

Dronc^I29/Dronc^I29 is a suppressor of wing disc | third instar larval stage phenotype of Ipk2^20B

(Seeds et al., 2015)

Dronc[+]/Dronc^I29 is a suppressor of eye phenotype of Scer\GAL4^GMR.PU, Srrm1^GE25979

(Fan et al., 2014)

Dronc^I29 is a suppressor | somatic clone of eye disc | posterior | third instar larval stage phenotype of Scer\GAL4^GMR.PU, p53^GUS.PB

(Fan et al., 2010)

Dronc^I29/Dronc^I29 is a suppressor of eye phenotype of Diap1^RNAi.UAS.cLa, Scer\GAL4^GMR.PF

(Leulier et al., 2006)

Dronc^I29/Dronc^I29 is a suppressor of interommatidial bristle phenotype of Diap1^RNAi.UAS.cLa, Scer\GAL4^GMR.PF

(Leulier et al., 2006)

Dronc^I29 is a suppressor of prothoracic leg disc phenotype of Scer\GAL4^Act5C.PI, wg^UAS.cLa

(Wells et al., 2006)

Dronc^I29/Dronc^I29 is a suppressor of eye | somatic clone phenotype of hid^GMR.PG

(Xu et al., 2005)

NOT Suppressor of

Statement

Reference

Dronc^I29/Dronc^I29 is a non-suppressor of somatic cell of testis | somatic clone phenotype of Rbf^GL01293, Scer\GAL4^Tub.PU

(Sainz de la Maza et al., 2022)

Dronc^I29/Dronc^I29 is a non-suppressor of eye phenotype of Ire1^UAS.Tag:V5, Scer\GAL4^GMR.long

(Yan et al., 2019)

shi^7C7/Dronc^I29 is a non-suppressor of eye disc | somatic clone | third instar larval stage phenotype of Vps4^3B1

(Legent et al., 2015)

shi^7C7/Dronc^I29 is a non-suppressor of photoreceptor | somatic clone | third instar larval stage phenotype of Vps4^3B1

(Legent et al., 2015)

shi^FL54/Dronc^I29 is a non-suppressor of eye disc | somatic clone | third instar larval stage phenotype of Vps4^3B1

(Legent et al., 2015)

shi^FL54/Dronc^I29 is a non-suppressor of photoreceptor | somatic clone | third instar larval stage phenotype of Vps4^3B1

(Legent et al., 2015)

Dronc^I29 is a non-suppressor | somatic clone of photoreceptor cell R7 | somatic clone | third instar larval stage phenotype of Scer\GAL4^GMR.PU, p53^GUS.PB

(Fan et al., 2010)

Other

Statement

Reference

Dronc^I29, Strica⁴ has egg chamber | germline clone phenotype

(Baum et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

The small eye phenotype resulting from the cell death induced by expression of hid^GMR.PG is partially suppressed by combination with Dronc^I29.

(Neukomm et al., 2017)

Dronc^I29 largely suppresses the increase in apoptosis seen in Vps4^3B1 mutant eye disc clones. The reduction in R8 photoreceptor number is also suppressed.

Vps4^3B1 suppresses the increase in photoreceptor number seen in shi^7C7 mutant eye disc clones, with the double mutants showing the photoreceptor loss seen in Vps4^3B1 mutants alone. The same is seen in a Dronc^I29 mutant background.

shi^FL54 does not suppress the photoreceptor loss seen in Vps4^3B1 mutant eye disc clones (in which cell death has been prevented using Dronc^I29).

(Legent et al., 2015)

Dronc^I29/Dronc^I29 suppresses increased cell death seen in Ipk2^20B/Ipk2^20B third instar larval wing discs.

(Seeds et al., 2015)

One copy of Dronc^I29 slightly suppresses the eye roughness seen when SRm160^GE25979 is expressed under the control of Scer\GAL4^GMR.PU.

(Fan et al., 2014)

Dcp-1^Prev1; Nc^I29 double mutants exhibit an arrest of vCrz programmed cell death with all 16 vCrz neurons retained at 7 and 16 hours after puparium formation, compared to apoptosis of these neurons in wild-type controls.

(Lee et al., 2011)

Induction of Nc^I29 third instar larval eyes disc clones blocks the cell death seen when p53^GUS.PB is expressed under the control of Scer\GAL4^GMR.PU. However the reduction in R7 photoreceptors is not rescued.

(Fan et al., 2010)

Dcp-1^Prev1 ; Nc^I24/Nc^I29 Ice^Δ1 triple mutant larvae undergo cell death with morphology similar to the midgut of wild-type animals when analysed from -4 to -1 hours relative to puparium formation.

dream⁴ ; Nc^I24/Nc^I29 Ice^Δ1 triple mutant larvae show high levels of TUNEL staining at -4 to -1 hours relative to puparium formation, suggesting that they are undergoing cell death.

(Denton et al., 2009)

Females containing dream⁴ ; Nc^I29 double homozygous germline clones are fertile, but their ovaries contain 46% of mid-stage egg chambers with missing follicle cells and large surviving nurse cells. Many of these egg chambers contain excessive numbers of nurse cells. There is also an increase in mid-stage egg chambers containing condensed nurse cell nuclei but lacking follicle cells. 21% of stage 14 egg chambers contain persisting nurse cell nuclei.

No significant defects is seen in the egg chambers of females containing dream²/dream⁴ ; Nc^I29 double homozygous germline clones.

(Baum et al., 2007)

The persistence of salivary gland tissue that is seen at 24 hours hours after puparium formation when Pi3K92E^{Scer\UAS.T:Hsap\MYC} is expressed under the control of Scer\GAL4^fkh.PH is significantly enhanced if the animals are also carrying Nc^I24/Nc^I29.

(Berry and Baehrecke, 2007)

While 59% of Scer\GAL4^Act5C.PI>wg^Scer\UAS.cLa prothoracic leg discs form a blastema, only 15% of Scer\GAL4^Act5C.PI>wg^Scer\UAS.cLa, Nc^I29 legs form a blastema.

(Wells et al., 2006)

Xenogenetic Interactions

Statement

Reference

Nc^I29/+ suppresses the increase in the number of TUNEL-positive cells seen in the wing discs in larvae expressing Rcom\RA^cs2.Scer\UAS under the control of Scer\GAL4^en-e16E at 30[o]C. It also largely rescues the non-autonomous increase in BrdU incorporation which is seen in the non-expressing compartment in discs expressing Rcom\RA^cs2.Scer\UAS under the control of Scer\GAL4^en-e16E at 30[o]C. Nc^I29/+ does not rescue the autonomous or the non-autonomous reduction in wing area caused by expression of Rcom\RA^cs2.Scer\UAS under the control of Scer\GAL4^en-e16E.

(Mesquita et al., 2010)

'Undead' Scer\GAL4^en-e16E>W^Scer\UAS.cYa, BacA\p35^Scer\UAS.cHa cells in the posterior of the wing disc show no overgrowth phenotype in a Nc^I29 background.

(Wells et al., 2006)

Complementation and Rescue Data

Rescued by

Dronc^I29/Dronc^L32 is rescued by Dronc^pro.UAS

(Napoletano et al., 2017)

Dronc^I29/Dronc^L32 is rescued by Dronc^pro.C-A.UAS

(Napoletano et al., 2017)

Dronc^I29/Dronc^L32 is rescued by Dronc^ΔN.UAS

(Napoletano et al., 2017)

Dronc^I29/Dronc^L32 is rescued by Dronc^ΔN.C-A.UAS

(Napoletano et al., 2017)

Partially rescued by

Dronc^I29/Dronc^L32 is partially rescued by Dronc^WT.5'3'UTR

(Napoletano et al., 2017)

Dronc^I29/Dronc^L32 is partially rescued by Dronc^C-A.5'3'UTR

(Napoletano et al., 2017)

Not rescued by

Dronc^I29/Dronc^L32 is not rescued by Dronc^CARD.UAS

(Napoletano et al., 2017)

Comments

Both the significant proportion of hyperplastic adult testes and the increased necrosis at the apical tip of the testes observed in Dronc^I29/Dronc^L32 transheterozygotes are rescued by the expression of Dronc^pro.Scer\UAS, Dronc^{pro.C-A.Scer\UAS}, Dronc^ΔN.Scer\UAS, or Dronc^{ΔN.C-A.Scer\UAS}, but not Dronc^{CARD.Scer\UAS}, under the control of Scer\GAL4^nos.PU. The significant proportion of hyperplastic adult testes is also rescued by the expression of either Dronc^WT.5'3'UTR or Dronc^C-A.5'3'UTR.

The sperm individualization defects observed in Dronc^I29/Dronc^L32 transheterozygotes are not rescued by the expression of either Dronc^WT.5'3'UTR or Dronc^C-A.5'3'UTR.

(Napoletano et al., 2017)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

99695

w^*; Dronc^I29 P{neoFRT}80B/TM3, Sb¹

98453

w^*; Dronc^I29 P{FRT(w^hs)}2A/TM3, Sb¹

Notes on Origin

Discoverer

Selected as: a recessive suppressor of the W^GMR.PG eye ablation phenotype.

(Xu et al., 2005)

Selected as: a mutant that recessively suppresses the eye ablation phenotype caused by eye-specific overexpression of W.

(Arama et al., 2006)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

Dronc^I29

(Sainz de la Maza et al., 2022, Arthurton et al., 2020, Yan et al., 2019, Vishal et al., 2018, Suisse et al., 2017, Ding et al., 2016, Legent et al., 2015, Huang et al., 2014)

Dronc^L29

(Mesquita et al., 2010)

Nc^I29

(Yacobi-Sharon et al., 2013)

drc^I29

(Legent et al., 2015)

dronc¹²⁹

(Krieser et al., 2007)

dronc^l29

(Mesquita et al., 2010)

Name Synonyms

droncI29

(Leulier et al., 2006)

droncl29

(Leulier et al., 2006)

Secondary FlyBase IDs

References (37)

Report Sections

Open Close