FlyBase Allele Report: Dmel\Myc[UAS.Tag:HA]

General Information

Symbol

Dmel\Myc^UAS.Tag:HA

Species

D. melanogaster

Name

FlyBase ID

FBal0190361

Feature type

allele

Associated gene

Dmel\Myc

Associated Insertion(s)

Carried in Construct

P{UAS-Myc.HA}

Also Known As

UAS-HA-dMyc, UAS-HAdm

Key Links

Transgenic product class

wild_type

(Bellosta et al., 2005)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Bellosta et al., 2005)

Mutagen

in vitro construct

(Bellosta et al., 2005)

Progenitor genotype

Carried in construct

P{UAS-Myc.HA}

Cytology

Description

UAS regulatory sequences drive expression of Myc tagged with Tag:HA.

(Bellosta et al., 2005)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Myc

Tagged with

Tag:HA

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of carcinoma

CEA with l(2)gl^HMS01522

(Sollazzo et al., 2022)

model of cancer

CEA

(Sollazzo et al., 2018)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates carcinoma

modeled by l(2)gl⁴

(Sollazzo et al., 2022)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal starvation stress response, with Scer\GAL4^Cg.PA

(Parisi et al., 2013)

increased body size, with Scer\GAL4^Cg.PA

(Parisi et al., 2013)

increased cell number, with Scer\GAL4^Cg.PA

(Parisi et al., 2013)

increased cell size, with Scer\GAL4^Cg.PA

(Parisi et al., 2013)

increased cell size, with Scer\GAL4^ppl.PU

(Parisi et al., 2013)

Phenotype Manifest In

lipid droplet, with Scer\GAL4^Cg.PA

(Parisi et al., 2013)

ommatidium, with Scer\GAL4^Cg.PA

(Parisi et al., 2013)

wing, with Scer\GAL4^Cg.PA

(Parisi et al., 2013)

wing, with Scer\GAL4^ppl.PU

(Parisi et al., 2013)

Detailed Description

Statement

Reference

Scer\GAL4^Cg.PA Myc^{Scer\UAS.T:Ivir\HA1} animals develop with a similar rate as controls, but they reach pupation at a significantly bigger size than controls. Adults are 17% heavier than controls. Scer\GAL4^Cg.PA Myc^{Scer\UAS.T:Ivir\HA1} flies have larger wings owing to an increased size and number of cells. Scer\GAL4^Cg.PA Myc^{Scer\UAS.T:Ivir\HA1} flies also have larger ommatidia. Scer\GAL4^ppl.PU Myc^{Scer\UAS.T:Ivir\HA1} flies have larger wings comprising larger cells.

Scer\GAL4^Hml.PG- or Scer\GAL4^srp.Hemo-mediated expression of Myc^{Scer\UAS.T:Ivir\HA1} does not affect developmental timing or body/wing size.

Fat cells in the fat bodies of Scer\GAL4^Cg.PA Myc^{Scer\UAS.T:Ivir\HA1} animals contain cytoplasmic lipid droplets smaller in size than controls. The fat cells are larger and contain more triglyceride compared to controls.

The half-life of Scer\GAL4^Cg.PA Myc^{Scer\UAS.T:Ivir\HA1} animals during starvation is increased compared to controls.

(Parisi et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Myc^UAS.Tag:HA, Scer\GAL4^Cg.PA has abnormal starvation stress response phenotype, suppressible by Desat1^GD2950, Scer\GAL4^Cg.PA

(Parisi et al., 2013)

Myc^UAS.Tag:HA, Scer\GAL4^Cg.PA has abnormal size phenotype, suppressible by Ilp5¹/Df(3L)Ilp2-3

(Parisi et al., 2013)

Myc^UAS.Tag:HA, Scer\GAL4^Cg.PA has increased body size | pupal stage phenotype, suppressible by Desat1^GD2950, Scer\GAL4^Cg.PA

(Parisi et al., 2013)

NOT suppressed by

Statement

Reference

Myc^UAS.Tag:HA, Scer\GAL4^Cg.PA has increased body size | pupal stage phenotype, non-suppressible by Pyk^GD16178, Scer\GAL4^Cg.PA

(Parisi et al., 2013)

Myc^UAS.Tag:HA, Scer\GAL4^Cg.PA has increased body size | pupal stage phenotype, non-suppressible by Hex-C^GD12378, Scer\GAL4^Cg.PA

(Parisi et al., 2013)

NOT Enhancer of

Statement

Reference

Scer\GAL4^Tub.PU/Myc^UAS.Tag:HA is a non-enhancer of decreased size | larval stage | somatic clone phenotype of Shmt^m3-5

(Silva et al., 2023)

NOT Suppressor of

Statement

Reference

Scer\GAL4^Tub.PU/Myc^UAS.Tag:HA is a non-suppressor of decreased size | somatic clone | larval stage phenotype of Shmt^m3-5

(Silva et al., 2023)

Other

Statement

Reference

Myc^UAS.Tag:HA, Scer\GAL4^Tub.PU, l(2)gl⁴ has increased cell size | somatic clone phenotype

(Froldi et al., 2010)

Myc^UAS.Tag:HA, Scer\GAL4^Tub.PU, l(2)gl⁴ has increased cell number phenotype

(Froldi et al., 2010)

Myc^UAS.Tag:HA, Scer\GAL4^Tub.PU, l(2)gl⁴ has increased cell death | somatic clone | cell non-autonomous phenotype

(Froldi et al., 2010)

Myc^UAS.Tag:HA, Scer\GAL4^Tub.PU, l(2)gl⁴ has lethal - all die before end of pupal stage | somatic clone phenotype

(Froldi et al., 2010)

Phenotype Manifest In

Suppressed by

Statement

Reference

Myc^UAS.Tag:HA, Scer\GAL4^Cg.PA has wing phenotype, suppressible by Ilp5¹/Df(3L)Ilp2-3

(Parisi et al., 2013)

Myc^UAS.Tag:HA, Scer\GAL4^Cg.PA has wing phenotype, suppressible by Desat1^GD2950, Scer\GAL4^Cg.PA

(Parisi et al., 2013)

NOT suppressed by

Statement

Reference

Myc^UAS.Tag:HA, Scer\GAL4^Cg.PA has wing phenotype, non-suppressible by Pyk^GD16178, Scer\GAL4^Cg.PA

(Parisi et al., 2013)

Myc^UAS.Tag:HA, Scer\GAL4^Cg.PA has wing phenotype, non-suppressible by Hex-C^GD12378, Scer\GAL4^Cg.PA

(Parisi et al., 2013)

NOT Enhancer of

Statement

Reference

Scer\GAL4^Tub.PU/Myc^UAS.Tag:HA is a non-enhancer of optic lobe neuroepithelial cell | larval stage | somatic clone phenotype of Shmt^m3-5

(Silva et al., 2023)

NOT Suppressor of

Statement

Reference

Scer\GAL4^Tub.PU/Myc^UAS.Tag:HA is a non-suppressor of optic lobe neuroepithelial cell | somatic clone | larval stage phenotype of Shmt^m3-5

(Silva et al., 2023)

Other

Statement

Reference

Myc^UAS.Tag:HA, Scer\GAL4^Tub.PU, l(2)gl⁴ has wing disc | somatic clone phenotype

(Froldi et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Scer\GAL4^Cg.PA-mediated expression of Myc^{Scer\UAS.T:Ivir\HA1} does not result in large wings in a Df(3L)Ilp2-3, Ilp5¹ background.

Co-expression of Desat1^GD2950, but not PyK^GD16178 or Hex-C^GD12378, prevents the Scer\GAL4^Cg.PA-mediated expression of Myc^{Scer\UAS.T:Ivir\HA1} resulting in abnormally large pupae and large wings. Co-expression of Desat1^GD2950 also prevents the increased half-life upon starvation seen in Scer\GAL4^Cg.PA Myc^{Scer\UAS.T:Ivir\HA1} animals.

(Parisi et al., 2013)

Expression of Myc^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^tub.PU suppresses the reduction in l(2)gl⁴ mutant wing disc clone size compared with controls. The clones are round in shape and massively overgrown, with the largest clones located in the proximal regions of the disc (the hinge and pleura). Cells in these clones are larger than in l(2)gl⁴ clones, but this gain in cell size only accounts for a small fraction of clone expansion. The majority of the large clones show little or no apoptosis, but some apoptosis is seen in smaller clones and just outside of the overgrown clones. The cells exhibit defects in apical-basal polarity. There is some discontinuity across the basement membrane, suggesting the cells have invasive properties.

(Froldi et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^Cg.PA/Myc^UAS.Tag:HA rescues Myc^JF01762

(Parisi et al., 2013)

Comments

Co-expression of Myc^{Scer\UAS.T:Ivir\HA1} rescues the small size and lethality of Myc^JF01762 Scer\GAL4^Cg.PA animals.

(Parisi et al., 2013)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Myc^{Scer\UAS.T:Ivir\HA1}

Myc^UAS.Tag:HA

dm^{Scer\UAS.T:Ivir\HA1}

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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