FlyBase Allele Report: Hsap\RUNX1::Hsap\RUNX1T1[UAS.cMa]

General Information

Symbol

Hsap\RUNX1::Hsap\RUNX1T1^UAS.cMa

Species

H. sapiens

Name

Saccharomyces cerevisiae UAS construct a of Mathey-Prevot

FlyBase ID

FBal0190422

Feature type

allele

Associated gene

Hsap\RUNX1::Hsap\RUNX1T1

Associated Insertion(s)

Carried in Construct

P{UAS-Hsap\RUNX1::Hsap\RUNX1T1.M}

Also Known As

UAS-AML1-ETO, UAS-aml1eto

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Wildonger and Mann, 2005)

Progenitor genotype

Carried in construct

P{UAS-Hsap\RUNX1::Hsap\RUNX1T1.M}

Cytology

Description

UAS sequences drive expression of Hsap\RUNX1::Hsap\RUNX1T1. Hsap\RUNX1::Hsap\RUNX1T1 is a fusion protein that contains the N-terminus and RD domain of Hsap\RUNX1 and almost the entire coding region of Hsap\RUNX1T1.

(Wildonger and Mann, 2005)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Hsap\RUNX1::Hsap\RUNX1T1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of acute myeloid leukemia

CEA

(Osman et al., 2009)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of acute myeloid leukemia

is ameliorated by CalpB^NIG.cUa

(Osman et al., 2009)

is ameliorated by CalpB^e04062

(Osman et al., 2009)

is ameliorated by pont^NIG.4003R

(Breig et al., 2014)

is ameliorated by pont^KK101103

(Breig et al., 2014)

Comments on Models/Modifiers Based on Experimental Evidence ( 1 )

The Drosophila eye has been used as an in vivo system to investigate the molecular function of the AML1-ETO chimeric protein (which is associated with acute myelogenous leukemia in humans). The AML1-ETO chimeric protein appears to act as a constitutive transcriptional repressor in this system.

(Wildonger and Mann, 2005)

Disease-implicated variant(s)

This allele represents a human variant implicated in disease.

(Wildonger and Mann, 2005)

RUNX1/RUNX1T1_fusion

(FlyBase curators, 2019-)

Variants Synonym(s)

Associated human disease model(s)

acute myeloid leukemia, RUNX1-RUNX1T1 fusion

External database links

COSMIC_fusion: RUNX1-RUNX1T1

Comments concerning this variant

Phenotypic Data

Phenotypic Class

hyperplasia | third instar larval stage, with Scer\GAL4^lz-gal4

(Bras et al., 2012)

lethal - all die before end of pupal stage, with Scer\GAL4^lz-gal4

(Osman et al., 2009, Wildonger and Mann, 2005)

some die during pupal stage, with Scer\GAL4^lz-gal4

(Osman et al., 2009, Wildonger and Mann, 2005)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of Hsap\RUNX1::Hsap\RUNX1T1^UAS.cMa in the larval blood cells under the control of Scer\GAL4^lz-gal4 causes an expansion of this hematopoietic lineage and blocks its differentiation into crystal cells.

(Breig et al., 2014)

Expression of Hsap\RUNX1::Hsap\RUNX1T1^Scer\UAS.cMa under the control of Scer\GAL4^lz-gal4 has leukemogenic activity in third instar larvae.

(Bras et al., 2012)

Expression of Hsap\RUNX1::Hsap\RUNX1T1^Scer\UAS.cMa under the control of Scer\GAL4^srp.D.cCa does not affect plasmatocyte development in the embryo, but inhibits crystal cell differentiation.

Expression of Hsap\RUNX1::Hsap\RUNX1T1^Scer\UAS.cMa under the control of Scer\GAL4^lz-gal4 prevents crystal cell differentiation in the embryo and larval lymph gland.

The number of circulating plasmatocytes in larvae expressing Hsap\RUNX1::Hsap\RUNX1T1^Scer\UAS.cMa under the control of Scer\GAL4^lz-gal4 is similar to that of control larvae. The circulating blood cells in the mutant larvae contain 19% differentiated crystal cells and 81% crystal cell progenitors, in contrast to wild type larvae (87% and 13% respectively). Differentiation of crystal cells can be partially restored in larvae expressing Hsap\RUNX1::Hsap\RUNX1T1^Scer\UAS.cMa under the control of Scer\GAL4^lz-gal4 if Scer\GAL80^ts.αTub84B is used to inhibit expression at the early third instar larval stage.

(Osman et al., 2009)

Flies expressing Hsap\RUNX1::Hsap\RUNX1T1^Scer\UAS.cMa under the control of Scer\GAL4^lz-gal4 that survive to late pupal stages have glazed eyes in which individual ommatidia cannot be observed. In these flies, part of the eye is frequently covered by scar tissue. In flies that express Hsap\RUNX1::Hsap\RUNX1T1^Scer\UAS.cMa under the control of Scer\GAL4^GMR.PF, eyes are greatly reduced in size and no ommatidia are visible. There is no evidence of a cone cell-to-photoreceptor transformation in eye discs that express Hsap\RUNX1::Hsap\RUNX1T1^Scer\UAS.cMa under the control of Scer\GAL4^GMR.PF. There is a small reduction in the number of cone cells in larval and pupal eyes expressing Hsap\RUNX1::Hsap\RUNX1T1^Scer\UAS.cMa under the control of Scer\GAL4^lz-gal4.

(Wildonger and Mann, 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Hsap\RUNX1::Hsap\RUNX1T1^UAS.cMa, Scer\GAL4^lz-gal4 has lethal - all die before end of pupal stage phenotype, suppressible by pont^NIG.4003R, Scer\GAL4^lz-gal4

(Breig et al., 2014)

Hsap\RUNX1::Hsap\RUNX1T1^UAS.cMa, Scer\GAL4^lz-gal4 has lethal - all die before end of pupal stage phenotype, suppressible by pont^KK101103, Scer\GAL4^lz-gal4

(Breig et al., 2014)

Hsap\RUNX1::Hsap\RUNX1T1^UAS.cMa, Scer\GAL4^lz-gal4 has hyperplasia | third instar larval stage phenotype, suppressible by Mlf^Δ5-3

(Bras et al., 2012)

Hsap\RUNX1::Hsap\RUNX1T1^UAS.cMa, Scer\GAL4^lz-gal4 has lethal | pupal stage phenotype, suppressible by CalpB^NIG.cUa, Scer\GAL4^lz-gal4

(Osman et al., 2009)

Phenotype Manifest In

Enhanced by

Statement

Reference

Hsap\RUNX1::Hsap\RUNX1T1^UAS.cMa, Scer\GAL4^GMR.PF has eye phenotype, enhanceable by Bro^UAS.cLa, Scer\GAL4^GMR.PF

(Wildonger and Mann, 2005)

Hsap\RUNX1::Hsap\RUNX1T1^UAS.cMa, Scer\GAL4^GMR.PF has eye phenotype, enhanceable by Bgb^UAS.cLa, Scer\GAL4^GMR.PF

(Wildonger and Mann, 2005)

Suppressed by

Statement

Reference

Hsap\RUNX1::Hsap\RUNX1T1^UAS.cMa, Scer\GAL4^lz-gal4 has embryonic/larval crystal cell | larval stage phenotype, suppressible by pont^KK101103, Scer\GAL4^lz-gal4

(Breig et al., 2014)

Hsap\RUNX1::Hsap\RUNX1T1^UAS.cMa, Scer\GAL4^lz-gal4 has embryonic/larval hemocyte | increased number | larval stage phenotype, suppressible by pont^NIG.4003R, Scer\GAL4^lz-gal4

(Breig et al., 2014)

Hsap\RUNX1::Hsap\RUNX1T1^UAS.cMa, Scer\GAL4^lz-gal4 has embryonic/larval crystal cell | larval stage phenotype, suppressible by pont^NIG.4003R, Scer\GAL4^lz-gal4

(Breig et al., 2014)

Hsap\RUNX1::Hsap\RUNX1T1^UAS.cMa, Scer\GAL4^lz-gal4 has embryonic/larval hemocyte | increased number | larval stage phenotype, suppressible by pont^KK101103, Scer\GAL4^lz-gal4

(Breig et al., 2014)

Hsap\RUNX1::Hsap\RUNX1T1^UAS.cMa, Scer\GAL4^lz-gal4 has embryonic/larval crystal cell phenotype, suppressible by CalpB^NIG.cUa, Scer\GAL4^lz-gal4

(Osman et al., 2009)

Hsap\RUNX1::Hsap\RUNX1T1^UAS.cMa, Scer\GAL4^lz-gal4 has embryonic/larval crystal cell phenotype, suppressible by Df(3L)CalpB^-/Df(3L)CalpB^-

(Osman et al., 2009)

Hsap\RUNX1::Hsap\RUNX1T1^UAS.cMa, Scer\GAL4^lz-gal4 has embryonic/larval crystal cell phenotype, suppressible by lz^UAS.cBa, Scer\GAL4^lz-gal4

(Osman et al., 2009)

Hsap\RUNX1::Hsap\RUNX1T1^UAS.cMa, Scer\GAL4^GMR.PF has eye phenotype, suppressible | partially by +/Df(3L)Bgb-K4

(Wildonger and Mann, 2005)

Hsap\RUNX1::Hsap\RUNX1T1^UAS.cMa, Scer\GAL4^GMR.PF has eye phenotype, suppressible by lz^UAS.cBa, Scer\GAL4^GMR.PF

(Wildonger and Mann, 2005)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Expression of pont^NIG.4003R suppresses the lethality seen when Hsap\RUNX1::Hsap\RUNX1T1^UAS.cMa is expressed under the control of Scer\GAL4^lz-gal4. The increase in lz positive larval hematopoietic cells is suppressed and differentiation into crystal cells is partially restored.

Expression of pont^KK101103 suppresses the lethality seen when Hsap\RUNX1::Hsap\RUNX1T1^UAS.cMa is expressed under the control of Scer\GAL4^lz-gal4. The increase in lz positive larval hematopoietic cells is suppressed and differentiation into crystal cells is partially restored.

(Breig et al., 2014)

The leukemogenic phenotype of Hsap\RUNX1::Hsap\RUNX1T1^Scer\UAS.cMa-expression via Scer\GAL4^lz-gal4 is almost abolished in Mlf^Δ5-3 mutant larvae.

(Bras et al., 2012)

Differentiation of crystal cells is restored in larvae expressing Hsap\RUNX1::Hsap\RUNX1T1^Scer\UAS.cMa under the control of Scer\GAL4^lz-gal4 if they are also co-expressing CalpB^NIG.cUa.

Differentiation of crystal cells is significantly restored in larvae expressing Hsap\RUNX1::Hsap\RUNX1T1^Scer\UAS.cMa under the control of Scer\GAL4^lz-gal4 if they are also co-expressing lz^Scer\UAS.cBa.

Differentiation of crystal cells is restored in larvae expressing Hsap\RUNX1::Hsap\RUNX1T1^Scer\UAS.cMa under the control of Scer\GAL4^lz-gal4 in a Df(3L)CalpB^-/Df(3L)CalpB^- background.

(Osman et al., 2009)

Coexpression of Bro^Scer\UAS.cLa or Bgb^Scer\UAS.cLa increases the severity of the Scer\GAL4^GMR.PF>Hsap\RUNX1::Hsap\RUNX1T1^Scer\UAS.cMa phenotype at 22^oC leading to eyes that are greatly reduced in size.

In flies heterozygous for Df(3L)Bgb-K4, the Scer\GAL4^GMR.PF>Hsap\RUNX1::Hsap\RUNX1T1^Scer\UAS.cMa phenotype is partially suppressed.

Coexpression of lz^Scer\UAS.cBa suppresses the Scer\GAL4^GMR.PF>Hsap\RUNX1::Hsap\RUNX1T1^Scer\UAS.cMa eye phenotype.

(Wildonger and Mann, 2005)

Expressing Bro^Scer\UAS.cLa or Bgb^Scer\UAS.cLa increases the severity of the Scer\GAL4^GMR.PF>Hsap\RUNX1::Hsap\RUNX1T1^Scer\UAS.cMa phenotype at 22^oC leading to eyes that are greatly reduced in size. In flies that carry one copy of the Df(3L)Bgb-K4 mutation, the Scer\GAL4^GMR.PF>Hsap\RUNX1::Hsap\RUNX1T1^Scer\UAS.cMa phenotype is partially suppressed. Coexpression of lz^Scer\UAS.cBa suppresses the Scer\GAL4^GMR.PF>Hsap\RUNX1::Hsap\RUNX1T1^Scer\UAS.cMa eye phenotype

(Wildonger and Mann, 2005)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

B. Mathey-Prevot

(Wildonger and Mann, 2005)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Hsap\RUNX1::Hsap\RUNX1T1^Scer\UAS.cMa

Hsap\RUNX1::Hsap\RUNX1T1^UAS.cMa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Mathey-Prevot

(Wildonger and Mann, 2005)

Secondary FlyBase IDs

References (5)

Report Sections

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