FB2025_01 , released February 20, 2025
Allele: Dmel\Gcn5RNAi.UAS
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General Information
Symbol
Dmel\Gcn5RNAi.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0190529
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Transgenic product class
Nature of the Allele
Transgenic product class
Progenitor genotype
Carried in construct
Cytology
Description

UAS sequences drive expression of a 850bp fragment of Gcn5 (nts 1203-2053 of GenBank Accession NM140329) organised as an inverted repeat.

Allele components
Component
Use(s)
Encoded product / tool
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DNA sequence
Protein sequence
 
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

The posterior compartments od PcafdsRNA.Scer\UAS; Scer\GAL4en-e16E wing discs are often smaller than wild-type and appear flatter than anterior compartments. This change in compartment size is not due to decreased cell division - there is actually an increase in cells in mitosis and S-phase in the posterior of these discs. However there is a significant increase in apoptosis. Although most animals of this genotype die as pupae, a few survive to adulthood. In these animals, the posterior of the wings is reduced and has abnormal veins. There is no change in cell density in the posterior compartments of these wings, indicating that cell number is reduced. In the most dramatic cases, a bubble indicative of abnormal adhesion between the dorsal and ventral wing epithelial sheets is present. PcafdsRNA.Scer\UAS; Scer\GAL4vg.boundary animals exhibit loss of wing margin structures and reduction in size of the distal tarsus. PcafdsRNA.Scer\UAS; Scer\GAL4esg-NP5153 animals die as pharate adults with partial to complete absence of abdominal cuticle.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
Gcn5RNAi.UAS
Gcn5dsRNA.Scer\UAS
Gcn5dsRNA.UAS
PcafdsRNA.Scer\UAS
Name Synonyms
Secondary FlyBase IDs
    References (7)