cuticle & abdomen & pharate adult, with Scer\GAL4NP5153
The posterior compartments od PcafdsRNA.Scer\UAS; Scer\GAL4en-e16E wing discs are often smaller than wild-type and appear flatter than anterior compartments. This change in compartment size is not due to decreased cell division - there is actually an increase in cells in mitosis and S-phase in the posterior of these discs. However there is a significant increase in apoptosis. Although most animals of this genotype die as pupae, a few survive to adulthood. In these animals, the posterior of the wings is reduced and has abnormal veins. There is no change in cell density in the posterior compartments of these wings, indicating that cell number is reduced. In the most dramatic cases, a bubble indicative of abnormal adhesion between the dorsal and ventral wing epithelial sheets is present. PcafdsRNA.Scer\UAS; Scer\GAL4vg.boundary animals exhibit loss of wing margin structures and reduction in size of the distal tarsus. PcafdsRNA.Scer\UAS; Scer\GAL4esg-NP5153 animals die as pharate adults with partial to complete absence of abdominal cuticle.