Hsap\RELA^{AD.R92A10.T:Zzzz\Zip+} Scer\GAL4^{DBD.R17D06.T:Zzzz\Zip-}-mediated expression of Bhal\NaChBac^Scer\UAS.cNa does not trigger basal rhythmic depolarization in electroretinograms.

Expression of Hsap\KCNJ2^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^P2.4.Pdf results in the induction of large, long-duration action potentials in the large lateral ventral circadian clock neurons (lLN[[V]]s).

Expression of Bhal\NaChBac^Scer\UAS.cNa in TrpA1 neurons, under the control of Scer\GAL4^TrpA1-GAL4, results in reduced morning activity, even though TrpA1 is not expressed in the morning cells. These animals show a significant reduction in MAI compared to wild-type, but the EAI of these flies is comparable to controls.

Morning peaks in locomotor activity are present in Scer\GAL4^TrpA1-GAL4->Bhal\NaChBac^Scer\UAS.cNa flies at 25[o]C and 29[o]C, although they are nearly absent at 18[o]C. The morning activity of these flies at 25[o]C and 29[o]C is sustained during daytime and the evening peaks are sharply increased. Approximately 35% of Scer\GAL4^TrpA1-GAL4->Bhal\NaChBac^Scer\UAS.cNa flies show arrhythmic behaviour, whereas the remaining flies show low rhythmic power at 29[o]C.

Expression of Bhal\NaChBac^Scer\UAS.cNa under the control of Scer\GAL4^Ih.3.2 results in a wing extension defect, with 48% of flies having unexpanded wings and 9% of flies having partially extended wings.

Expression of Bhal\NaChBac^Scer\UAS.cNa under the control of Scer\GAL4^Ast.2.1 significantly reduces the fraction of flies that feed within 1 hour following 24 hours starvation. This reduced feeding behaviour is largely (but not completely) rescued if the flies also carry Scer\GAL4^npf.1.

Flies expressing Bhal\NaChBac^Scer\UAS.cNa under the control of Scer\GAL4^Ast.2.1 show normal average locomotor activity and starvation-enhanced locomotion. Energy store levels in these flies under starvation conditions are not significantly different from wild type.

Expression of Bhal\NaChBac^Scer\UAS.cNa under the control of Scer\GAL4^npf.1 significantly increases the fraction of flies that feed within 1 hour following 24 hours starvation.

Fed flies expressing Bhal\NaChBac^Scer\UAS.cNa under the control of Scer\GAL4^Ast.2.1 show a normal proboscis extension reflex (PER) across a range of sucrose concentrations. However, after starvation, these flies largely fail to show the enhanced PER response to sucrose seen in wild-type flies under these conditions.

The preference of starved Bhal\NaChBac^Scer\UAS.cNa, Scer\GAL4^Ast.2.1 flies for 5mM vs 1mM sucrose in a two-choice assay is not significantly different from wild type.

Expression of Bhal\NaChBac^Scer\UAS.cNa under the control of Scer\GAL4^upd-E132 results in defects in locomotor activity rhythms (only 26.7% of flies show rhythmic behaviour under constant darkness conditions).

Foraging third instar larvae expressing Bhal\NaChBac^Scer\UAS.cNa under the control of Scer\GAL4^P0.5.Pdf show robust light avoidance at 30 lux, with more than 60% of larvae choosing the dark side of a petri dish in a choice assay, while control larvae are not able to distinguish between light and dark at this low light level.

Males expressing Bhal\NaChBac^Scer\UAS.cNa under the control of Scer\GAL4^ppk23.2.336 show courtship towards wild-type males. Courtship towards females is unaltered.

Expression of Bhal\NaChBac^Scer\UAS.cNa in DA neurons under the control of Scer\GAL4^ple.PF causes a phase shift in circadian locomotor activity and gradually extends the circadian periodicity.

Expression of Bhal\NaChBac^Scer\UAS.cNa under the control of Scer\GAL4^repo.PU during the adult stage under constant darkness conditions results in the flies becoming arrhythmic within 1 day of expression (the temperature sensitive Scer\GAL80^ts.αTub84B allele is used to control the timing of expression by shifting the adults to 30[o]C at the beginning of the subjective daytime on day 1 of constant darkness).

Ventral lateral LN(v) neurons expressing Bhal\NaChBac^Scer\UAS.cNa display numerous peaks in calcium signals regardless of the presence or absence of light stimulation, indicative of elevated spontaneous activities compared with wild-type.

Expression of Bhal\NaChBac^Scer\UAS.cNa under the control of Scer\GAL4^Gad1.PS results in flies that are arrhythmic or that have significantly weaker rhythms than control flies.

 :Male flies expressing Bhal\NaChBac^Scer\UAS.cNa under the control of Scer\GAL4^Or67d-GAL4-1 show increased male-male aggression (they show a significantly increased number of lunges compared to controls).

Male flies expressing Bhal\NaChBac^Scer\UAS.cNa under the control of Scer\GAL4^Or65a.PF do not show a significantly increased number of lunges compared to controls.

Grouped flies expressing Bhal\NaChBac^Scer\UAS.cNa under the control of Scer\GAL4^Tdc2.PC during the adult stage show increased aggression after shifting to the restrictive temperature (which inactivates Scer\GAL80^ts.αTub84B and allows expression driven by Scer\GAL4^Tdc2.PC), with shorter fighting latency and higher lunging and high-intensity fighting (holding, boxing and tussling) frequencies.

Expression of Bhal\NaChBac^Scer\UAS.cNa, under the regulation of Scer\GAL4^P2.4.Pdf abolishes the cyclic accumulation of Pdf in dorsomedial lateral ventral subset LN^v neurons. Flies expressing Bhal\NaChBac^Scer\UAS.cNa in dorsomedial lateral ventral subset LN^v neurons, under the regulation of Scer\GAL4^P2.4.Pdf exhibit multiple, superimposed free-running circadian rhythms of locomotor activity with different periods, some of which are longer than 24hrs (e.g. 25.5hrs), some shorter (e.g. 22hrs). Some of the Bhal\NaChBac^Scer\UAS.cNa-expressing flies exhibit three significant rhythms, with periods of approximately 21 hrs, 22.5 hrs, and 25 hrs. There are no significant differents between controls and Bhal\NaChBac^Scer\UAS.cNa (Scer\GAL4^P2.4.Pdf) flies in overall locomotor activity.

Bhal\NaChBac^UAS.cNa, Scer\GAL4^AstA.2.1 has abnormal starvation stress response phenotype, suppressible by Hsap\KCNJ2^UAS.EGFP, Scer\GAL4^AstA.2.1

Bhal\NaChBac^UAS.cNa, Scer\GAL4^P2.4.Pdf has abnormal locomotor rhythm phenotype, suppressible by Hsap\KCNJ2^UAS.EGFP, Scer\GAL4^P2.4.Pdf

Scer\GAL4^Ilp2.PU/Bhal\NaChBac^UAS.cNa is a suppressor of abnormal developmental rate phenotype of vaha^del

Hsap\RELA^AD.R92A10, Bhal\NaChBac^UAS.cNa, Scer\GAL4^DBD.R17D06 is a suppressor | partially of abnormal neurophysiology phenotype of Ih^f03355

Bhal\NaChBac^UAS.cNa, Scer\GAL4^odd-MC is a suppressor of abnormal chemosensory behavior | larval stage phenotype of Hsap\KCNJ2^UAS.EGFP, Scer\GAL4^odd-MC

Bhal\NaChBac^UAS.cNa, Scer\GAL4^odd-MC is a suppressor of abnormal locomotor behavior | larval stage phenotype of Hsap\KCNJ2^UAS.EGFP, Scer\GAL4^odd-MC

Bhal\NaChBac^UAS.cNa, Scer\GAL4^P2.4.Pdf is a suppressor of abnormal locomotor rhythm phenotype of Hsap\KCNJ2^UAS.EGFP, Scer\GAL4^P2.4.Pdf