Expression of Bhal\NaChBacScer\UAS.cNa in TrpA1 neurons, under the control of Scer\GAL4TrpA1-GAL4, results in reduced morning activity, even though TrpA1 is not expressed in the morning cells. These animals show a significant reduction in MAI compared to wild-type, but the EAI of these flies is comparable to controls.
Morning peaks in locomotor activity are present in Scer\GAL4TrpA1-GAL4->Bhal\NaChBacScer\UAS.cNa flies at 25[o]C and 29[o]C, although they are nearly absent at 18[o]C. The morning activity of these flies at 25[o]C and 29[o]C is sustained during daytime and the evening peaks are sharply increased. Approximately 35% of Scer\GAL4TrpA1-GAL4->Bhal\NaChBacScer\UAS.cNa flies show arrhythmic behaviour, whereas the remaining flies show low rhythmic power at 29[o]C.
Expression of Bhal\NaChBacScer\UAS.cNa under the control of Scer\GAL4Ih.3.2 results in a wing extension defect, with 48% of flies having unexpanded wings and 9% of flies having partially extended wings.
Expression of Bhal\NaChBacScer\UAS.cNa under the control of Scer\GAL4Ast.2.1 significantly reduces the fraction of flies that feed within 1 hour following 24 hours starvation. This reduced feeding behaviour is largely (but not completely) rescued if the flies also carry Scer\GAL4npf.1.
Flies expressing Bhal\NaChBacScer\UAS.cNa under the control of Scer\GAL4Ast.2.1 show normal average locomotor activity and starvation-enhanced locomotion. Energy store levels in these flies under starvation conditions are not significantly different from wild type.
Expression of Bhal\NaChBacScer\UAS.cNa under the control of Scer\GAL4npf.1 significantly increases the fraction of flies that feed within 1 hour following 24 hours starvation.
Fed flies expressing Bhal\NaChBacScer\UAS.cNa under the control of Scer\GAL4Ast.2.1 show a normal proboscis extension reflex (PER) across a range of sucrose concentrations. However, after starvation, these flies largely fail to show the enhanced PER response to sucrose seen in wild-type flies under these conditions.
The preference of starved Bhal\NaChBacScer\UAS.cNa, Scer\GAL4Ast.2.1 flies for 5mM vs 1mM sucrose in a two-choice assay is not significantly different from wild type.
Expression of Bhal\NaChBacScer\UAS.cNa under the control of Scer\GAL4upd-E132 results in defects in locomotor activity rhythms (only 26.7% of flies show rhythmic behaviour under constant darkness conditions).
Foraging third instar larvae expressing Bhal\NaChBacScer\UAS.cNa under the control of Scer\GAL4P0.5.Pdf show robust light avoidance at 30 lux, with more than 60% of larvae choosing the dark side of a petri dish in a choice assay, while control larvae are not able to distinguish between light and dark at this low light level.
Expression of Bhal\NaChBacScer\UAS.cNa under the control of Scer\GAL4repo.PU during the adult stage under constant darkness conditions results in the flies becoming arrhythmic within 1 day of expression (the temperature sensitive Scer\GAL80ts.αTub84B allele is used to control the timing of expression by shifting the adults to 30[o]C at the beginning of the subjective daytime on day 1 of constant darkness).
Ventral lateral LN(v) neurons expressing Bhal\NaChBacScer\UAS.cNa display numerous peaks in calcium signals regardless of the presence or absence of light stimulation, indicative of elevated spontaneous activities compared with wild-type.
Grouped flies expressing Bhal\NaChBacScer\UAS.cNa under the control of Scer\GAL4Tdc2.PC during the adult stage show increased aggression after shifting to the restrictive temperature (which inactivates Scer\GAL80ts.αTub84B and allows expression driven by Scer\GAL4Tdc2.PC), with shorter fighting latency and higher lunging and high-intensity fighting (holding, boxing and tussling) frequencies.
Expression of Bhal\NaChBacScer\UAS.cNa, under the regulation of Scer\GAL4P2.4.Pdf abolishes the cyclic accumulation of Pdf in dorsomedial lateral ventral subset LNv neurons. Flies expressing Bhal\NaChBacScer\UAS.cNa in dorsomedial lateral ventral subset LNv neurons, under the regulation of Scer\GAL4P2.4.Pdf exhibit multiple, superimposed free-running circadian rhythms of locomotor activity with different periods, some of which are longer than 24hrs (e.g. 25.5hrs), some shorter (e.g. 22hrs). Some of the Bhal\NaChBacScer\UAS.cNa-expressing flies exhibit three significant rhythms, with periods of approximately 21 hrs, 22.5 hrs, and 25 hrs. There are no significant differents between controls and Bhal\NaChBacScer\UAS.cNa (Scer\GAL4P2.4.Pdf) flies in overall locomotor activity.