DJ-1αΔ72/+ dj-1βΔ93/dj-1βΔ93 significantly suppresses the small rough eye phenotype in RetMEN2A.GMR flies. DJ-1αΔ72/+, DJ-1αΔ72/DJ-1αΔ72 or DJ-1αΔ72/DJ-1αΔ72 dj-1βΔ93/+ significantly suppresses the small rough eye phenotype in RetMEN2B.GMR flies. DJ-1αΔ72/+ or DJ-1αΔ72/DJ-1αΔ72 significantly suppresses the increase in ommatidia size in RetMEN2B.GMR flies. DJ-1αΔ72/+ does not suppress the decreases in eye size seen in flies with expression of PtenScer\UAS.cKa driven by Scer\GAL4GMR.PU. dj-1βΔ93/+ DJ-1αΔ72/+ suppresses eye phenotypes (including increase in R cell number per ommatidium) in Ras85DG12V.sev flies.
DJ-1αΔ72/DJ-1αΔ72, dj-1βΔ93/dj-1βΔ93 double mutant flies have a reduced lifespan; display decreased spontaneous movement and climbing activity over time; have reduced fertility in both males and females; but have normal mitochondrial morphology, as compared with controls. Sperm from DJ-1αΔ72/DJ-1αΔ72, dj-1βΔ93/dj-1βΔ93 males appear morphologically normal but are immotile, have abnormal vacuoles in the Nebenkern, irregularities of mitochondrial derivatives, and misoriented and asynchronous progression of the investment cones during spermatogenesis. Mitochondria in DJ-1αΔ72/DJ-1αΔ72, dj-1βΔ93/dj-1βΔ93 double mutant flies show no difference in generating and maintaining cross-membrane potential, but do show a decline in mitochondrial function as the flies age, as measured by comparing the respiratory control ratio in 3 day- and 30 day-old whole flies.
DJ-1αΔ72; dj-1βΔ93 flies are viable, fertile and have no striking external phenotypes. There are no differences in the number of DA neurons in the fly brain between young (1 day) or aged (30 days) flies. DJ-1αΔ72; dj-1βΔ93 flies show a higher sensitivity to the oxidative agents paraquat and H2O2 than wild-type flies. However, the sensitivity of these double mutants to paraquat is not significantly higher than in dj-1βΔ93 single mutants. The double mutants do not show an increase in sensitivity to chemical agents that induce the unfolded protein response or that impair proteasome function. Expression of the either the dj-1βScer\UAS.cMa transgene or the DJ-1αScer\UAS.cMa transgene, under the control of Scer\GAL4αTub84B.PL, rescues the sensitivity of dj-1βΔ93; DJ-1αΔ72 mutants to paraquat.