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General Information
Symbol
Dmel\DJ-1αΔ72
Species
D. melanogaster
Name
FlyBase ID
FBal0190611
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

The entire coding region of DJ-1α is deleted.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

DJ-1αΔ72 mutant flies show elevated response across a range of spatiotemporal frequencies of contrast-reversing sine-wave gratings as compared to responses from control flies.

No rhabdomere loss is observed in DJ-1αΔ72 mutant flies.

DJ-1αΔ72/DJ-1αΔ72 mutants display normal eye development and ultrastructure.

DJ-1αΔ72/DJ-1αΔ72 mutants do not display any significant decrease in fertility.

DJ-1αΔ72 mutant adults (reared on a yeast-paste diet) show significantly decreased survival rate compared to wild-type controls.

DJ-1αΔ72 flies show no striking external phenotypes. These flies show no increase in sensitivity to agents that increase oxidative stress, or chemicals that induce the unfolded protein response or that impair proteasome function.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhancer of
Statement
Reference
Suppressor of
Statement
Reference

DJ-1alpha[+]/DJ-1αΔ72, dj-1βΔ93/dj-1βΔ93 is a suppressor of visible phenotype of RetMEN2A.GMR

dj-1βΔ93/dj-1beta[+], DJ-1αΔ72/DJ-1αΔ72 is a suppressor of visible phenotype of RetMEN2B.GMR

dj-1βΔ93, dj-1beta[+], DJ-1alpha[+], DJ-1αΔ72 is a suppressor of visible phenotype of Ras85DG12V.sev, Scer\GAL4GMR.PU

dj-1βΔ93, dj-1beta[+], DJ-1alpha[+], DJ-1αΔ72 is a suppressor of increased cell number | adult stage phenotype of Ras85DG12V.sev

DJ-1alpha[+]/DJ-1αΔ72 is a suppressor of visible phenotype of RetMEN2B.GMR

DJ-1alpha[+]/DJ-1αΔ72 is a suppressor of increased cell size | adult stage phenotype of RetMEN2B.GMR

NOT Suppressor of
Statement
Reference

DJ-1alpha[+]/DJ-1αΔ72 is a non-suppressor of visible phenotype of PtenUAS.cKa, Scer\GAL4GMR.PU

Other
Phenotype Manifest In
Suppressor of
Statement
Reference

DJ-1alpha[+]/DJ-1αΔ72, dj-1βΔ93/dj-1βΔ93 is a suppressor of eye | adult stage phenotype of RetMEN2A.GMR

dj-1βΔ93, dj-1beta[+], DJ-1alpha[+], DJ-1αΔ72 is a suppressor of eye phenotype of Ras85DG12V.sev

dj-1βΔ93, dj-1beta[+], DJ-1alpha[+], DJ-1αΔ72 is a suppressor of eye photoreceptor cell | supernumerary | adult stage phenotype of Ras85DG12V.sev

dj-1βΔ93, dj-1beta[+], DJ-1alpha[+], DJ-1αΔ72 is a suppressor of ommatidium phenotype of Ras85DG12V.sev

dj-1βΔ93/dj-1beta[+], DJ-1αΔ72/DJ-1αΔ72 is a suppressor of eye | adult stage phenotype of RetMEN2B.GMR

DJ-1alpha[+]/DJ-1αΔ72 is a suppressor of eye | adult stage phenotype of RetMEN2B.GMR

DJ-1alpha[+]/DJ-1αΔ72 is a suppressor of ommatidium | adult stage phenotype of RetMEN2B.GMR

NOT Suppressor of
Statement
Reference

DJ-1alpha[+]/DJ-1αΔ72 is a non-suppressor of eye phenotype of PtenUAS.cKa, Scer\GAL4GMR.PU

Other
Additional Comments
Genetic Interactions
Statement
Reference

DJ-1αΔ72/+ dj-1βΔ93/dj-1βΔ93 significantly suppresses the small rough eye phenotype in RetMEN2A.GMR flies. DJ-1αΔ72/+, DJ-1αΔ72/DJ-1αΔ72 or DJ-1αΔ72/DJ-1αΔ72 dj-1βΔ93/+ significantly suppresses the small rough eye phenotype in RetMEN2B.GMR flies. DJ-1αΔ72/+ or DJ-1αΔ72/DJ-1αΔ72 significantly suppresses the increase in ommatidia size in RetMEN2B.GMR flies. DJ-1αΔ72/+ does not suppress the decreases in eye size seen in flies with expression of PtenScer\UAS.cKa driven by Scer\GAL4GMR.PU. dj-1βΔ93/+ DJ-1αΔ72/+ suppresses eye phenotypes (including increase in R cell number per ommatidium) in Ras85DG12V.sev flies.

DJ-1αΔ72/DJ-1αΔ72, dj-1βΔ93/dj-1βΔ93 double mutant flies have a reduced lifespan; display decreased spontaneous movement and climbing activity over time; have reduced fertility in both males and females; but have normal mitochondrial morphology, as compared with controls. Sperm from DJ-1αΔ72/DJ-1αΔ72, dj-1βΔ93/dj-1βΔ93 males appear morphologically normal but are immotile, have abnormal vacuoles in the Nebenkern, irregularities of mitochondrial derivatives, and misoriented and asynchronous progression of the investment cones during spermatogenesis. Mitochondria in DJ-1αΔ72/DJ-1αΔ72, dj-1βΔ93/dj-1βΔ93 double mutant flies show no difference in generating and maintaining cross-membrane potential, but do show a decline in mitochondrial function as the flies age, as measured by comparing the respiratory control ratio in 3 day- and 30 day-old whole flies.

Expression of parkScer\UAS.cGa or Pink1Scer\UAS.cUa under the control of Scer\GAL4da.PU results in lethality in the DJ-1αΔ72/DJ-1αΔ72, dj-1βΔ93/dj-1βΔ93 double mutant background.

DJ-1αΔ72; dj-1βΔ93 flies are viable, fertile and have no striking external phenotypes. There are no differences in the number of DA neurons in the fly brain between young (1 day) or aged (30 days) flies. DJ-1αΔ72; dj-1βΔ93 flies show a higher sensitivity to the oxidative agents paraquat and H2O2 than wild-type flies. However, the sensitivity of these double mutants to paraquat is not significantly higher than in dj-1βΔ93 single mutants. The double mutants do not show an increase in sensitivity to chemical agents that induce the unfolded protein response or that impair proteasome function. Expression of the either the dj-1βScer\UAS.cMa transgene or the DJ-1αScer\UAS.cMa transgene, under the control of Scer\GAL4αTub84B.PL, rescues the sensitivity of dj-1βΔ93; DJ-1αΔ72 mutants to paraquat.

DJ-1αΔ72; dj-1βΔ93 flies show a higher sensitivity to the oxidative agents paraquat, H2O2 and rotenone than wild-type flies.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
    References (7)