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General Information
Symbol
Dmel\cryΔ5'.UAS.cDa
Species
D. melanogaster
Name
FlyBase ID
FBal0190621
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of truncated cry cDNA coding sequence (with a deletion between residues 521-540).

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of cryΔ5'.Scer\UAS.cDa, under the control of Scer\GAL4tim.PE in flies exposed to constant darkness results in the flies exhibiting a significant increase in period length. The free-running period for these flies is 1.5-2.7 hours longer than for controls. The free-running period of flies expressing cryΔ5'.Scer\UAS.cDa, under the control of Scer\GAL4tim.PE is the same in cry+ and cryb flies. Under constant high levels of light, flies expressing cryΔ5'.Scer\UAS.cDa, under the control of Scer\GAL4tim.PE remain synchronised for about 4 days (whereas control flies desynchronise and become arrhythmic after 1 or 2 days), but with a long period, suggesting that they are less sensitive to constant light. Flies expressing cryΔ5'.Scer\UAS.cDa, under the control of Scer\GAL4tim.PE phase-shift almost normally at ZT15 and ZT17, but at ZT19 remain in the delay zone, becoming nearly unresponsive at ZT21 and ZT23. Flies overexpressing cryΔ5'.Scer\UAS.cDa, under the control of Scer\GAL4tim.PE display a normal pattern of delay and advance but are more reactive to light pulses. cryb flies expressing cryΔ5'.Scer\UAS.cDa, under the control of Scer\GAL4tim.PE generate a significantly enhanced phase shift response to a short light pulse, compared to cryb and control flies at ZT15, but are essentially unresponsive at ZT21. cryb flies expressing cryΔ5'.Scer\UAS.cDa, under the control of Scer\GAL4tim.PE, unlike cryb mutants, do eventually desynchronise under constant light, and do phase-shift significantly in the delay zone of the phase-response curve.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Fails to rescue
Comments

Overexpression of cryΔ5'.Scer\UAS.cDa, under the regulation of Scer\GAL4tim.PE does not rescue the cryb phenotype, with the flies remaining synchronised for about 6 days, exhibiting a long period phenotype before becoming desynchronised.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
cryΔ5'.Scer\UAS.cDa
cryΔ5'.UAS.cDa
Name Synonyms
Secondary FlyBase IDs
    References (1)