FlyBase Allele Report: EBV\BZLF1[GMR.PA]

General Information

Symbol

EBV\BZLF1^GMR.PA

Species

L. Cercopithecine herpesvirus 15

Name

glass multimer reporter construct of Adamson

FlyBase ID

FBal0190744

Feature type

allele

Associated gene

EBV\BZLF1

Associated Insertion(s)

Carried in Construct

P{GMR-BZLF1.A}

Also Known As

GMR-Z

Transgenic product class

cDNA

(Adamson et al., 2005)

wild_type

(Adamson et al., 2005)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Adamson et al., 2005)

wild_type

(Adamson et al., 2005)

Mutagen

in vitro construct

(Adamson et al., 2005)

Progenitor genotype

Carried in construct

P{GMR-BZLF1.A}

Cytology

Description

GMR regulatory sequences drive expression of a EBV\BZLF1 cDNA.

(Adamson et al., 2005)

Allele components

Component

Use(s)

Regulatory region(s)

GMR

engineered transcription regulatory region

Encoded product / tool

EBV\BZLF1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of viral infectious disease

CEA

(Adamson et al., 2005)

model of infectious mononucleosis

CEA

(Adamson et al., 2005)

model of Epstein-Barr virus hepatitis

CEA

(Adamson et al., 2005)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of viral infectious disease

is ameliorated by 14-3-3ε^S-696

(Adamson and Lajeunesse, 2012)

is ameliorated by l(2)gd1^EY04750

(Adamson and Lajeunesse, 2012)

is ameliorated by hyd¹⁵

(Adamson and Lajeunesse, 2012)

is exacerbated by mTor^k17004

(Adamson et al., 2014, Adamson and Lajeunesse, 2012)

is exacerbated by mTor^ΔP

(Adamson et al., 2014, Adamson and Lajeunesse, 2012)

is exacerbated by rpr^GMR.PH

(Adamson and Lajeunesse, 2012)

is exacerbated by Ras85D^e1B

(Adamson and Lajeunesse, 2012)

is exacerbated by p53^GMR.Ex

(Adamson and Lajeunesse, 2012)

is exacerbated by dlg1^G0276

(Adamson and Lajeunesse, 2012)

is exacerbated by ago^EY01092

(Adamson and Lajeunesse, 2012)

is exacerbated by Rab5^KG05684

(Adamson and Lajeunesse, 2012)

model of infectious mononucleosis

is ameliorated by hyd¹⁵

(Adamson and Lajeunesse, 2012)

is ameliorated by 14-3-3ε^S-696

(Adamson and Lajeunesse, 2012)

is ameliorated by l(2)gd1^EY04750

(Adamson and Lajeunesse, 2012)

is exacerbated by mTor^k17004

(Adamson et al., 2014, Adamson and Lajeunesse, 2012)

is exacerbated by mTor^ΔP

(Adamson et al., 2014, Adamson and Lajeunesse, 2012)

is exacerbated by rpr^GMR.PH

(Adamson and Lajeunesse, 2012)

is exacerbated by Ras85D^e1B

(Adamson and Lajeunesse, 2012)

is exacerbated by p53^GMR.Ex

(Adamson and Lajeunesse, 2012)

is exacerbated by dlg1^G0276

(Adamson and Lajeunesse, 2012)

is exacerbated by ago^EY01092

(Adamson and Lajeunesse, 2012)

is exacerbated by Rab5^KG05684

(Adamson and Lajeunesse, 2012)

model of Epstein-Barr virus hepatitis

is ameliorated by l(2)gd1^EY04750

(Adamson and Lajeunesse, 2012)

is ameliorated by 14-3-3ε^S-696

(Adamson and Lajeunesse, 2012)

is ameliorated by hyd¹⁵

(Adamson and Lajeunesse, 2012)

is exacerbated by mTor^ΔP

(Adamson et al., 2014, Adamson and Lajeunesse, 2012)

is exacerbated by Ras85D^e1B

(Adamson and Lajeunesse, 2012)

is exacerbated by rpr^GMR.PH

(Adamson and Lajeunesse, 2012)

is exacerbated by mTor^k17004

(Adamson et al., 2014, Adamson and Lajeunesse, 2012)

is exacerbated by p53^GMR.Ex

(Adamson and Lajeunesse, 2012)

is exacerbated by dlg1^G0276

(Adamson and Lajeunesse, 2012)

is exacerbated by ago^EY01092

(Adamson and Lajeunesse, 2012)

is exacerbated by Rab5^KG05684

(Adamson and Lajeunesse, 2012)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | adult stage

(Adamson et al., 2005)

decreased occurrence of cell division | third instar larval stage

(Adamson et al., 2005)

increased cell death | third instar larval stage

(Adamson et al., 2005)

visible

(Adamson and Lajeunesse, 2012, Adamson et al., 2005)

Phenotype Manifest In

eye

(Adamson et al., 2014, Adamson and Lajeunesse, 2012, Adamson et al., 2005)

eye disc

(Adamson et al., 2005)

ommatidium

(Adamson and Lajeunesse, 2012)

Detailed Description

Statement

Reference

Expression of EBV\BZLF1^GMR.PA results in dose-dependent eye phenotypes including rough eye, diminished pigmentation, and loss of ommatidia.

(Adamson and Lajeunesse, 2012)

Flies carrying EBV\BZLF1^GMR.PA show loss of all eye structures except the interommatidial bristles, which are disorganised and scattered throughout the thin cuticle that remains in place of the eye. EBV\BZLF1^GMR.PA third larval instar eye discs show a significantly smaller number of cells which incorporate BrdU compared to wild-type discs and there is an increase in cell death in the discs compared to wild type. Flies carrying a weakly expressing P{GMR-BZLF1.A} line show a dose sensitive eye phenotype, with flies carrying one copy having a moderate eye phenotype with some eye tissue (but no recognisable ommatidia) and flies having two copies lacking all eye structures except the interommatidial bristles.

(Adamson et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

EBV\BZLF1^GMR.PA has visible phenotype, enhanceable by sv^spa-pol/sv[+]

(Adamson et al., 2005)

EBV\BZLF1^GMR.PA has abnormal size | adult stage phenotype, enhanceable by sv^spa-pol/sv[+]

(Adamson et al., 2005)

NOT Enhanced by

Statement

Reference

EBV\BZLF1^GMR.PA has visible phenotype, non-enhanceable by dap^GMR.PdN

(Adamson et al., 2005)

EBV\BZLF1^GMR.PA has visible phenotype, non-enhanceable by BacA\p35^GMR.PH

(Adamson et al., 2005)

EBV\BZLF1^GMR.PA has visible phenotype, non-enhanceable by CycE^AR95

(Adamson et al., 2005)

EBV\BZLF1^GMR.PA has visible phenotype, non-enhanceable by Rbf^GMR.PD

(Adamson et al., 2005)

NOT suppressed by

Statement

Reference

EBV\BZLF1^GMR.PA has abnormal size | adult stage phenotype, non-suppressible by CycE^AR95

(Adamson et al., 2005)

EBV\BZLF1^GMR.PA has abnormal size | adult stage phenotype, non-suppressible by BacA\p35^GMR.PH

(Adamson et al., 2005)

EBV\BZLF1^GMR.PA has visible phenotype, non-suppressible by dap^GMR.PdN

(Adamson et al., 2005)

EBV\BZLF1^GMR.PA has visible phenotype, non-suppressible by BacA\p35^GMR.PH

(Adamson et al., 2005)

EBV\BZLF1^GMR.PA has visible phenotype, non-suppressible by CycE^AR95

(Adamson et al., 2005)

EBV\BZLF1^GMR.PA has visible phenotype, non-suppressible by Rbf^GMR.PD

(Adamson et al., 2005)

EBV\BZLF1^GMR.PA has abnormal size | adult stage phenotype, non-suppressible by dap^GMR.PdN

(Adamson et al., 2005)

EBV\BZLF1^GMR.PA has abnormal size | adult stage phenotype, non-suppressible by Rbf^GMR.PD

(Adamson et al., 2005)

Suppressor of

Statement

Reference

EBV\BZLF1^GMR.PA is a suppressor of increased occurrence of cell division | larval stage phenotype of EBV\BRLF1^GMR.PA

(Adamson and Lajeunesse, 2012)

Other

Statement

Reference

EBV\BRLF1^GMR.PA, EBV\BZLF1^GMR.PA has visible phenotype

(Adamson and Lajeunesse, 2012)

Phenotype Manifest In

Enhanced by

Statement

Reference

EBV\BZLF1^GMR.PA has eye phenotype, enhanceable by Tor[+]/mTor^ΔP

(Adamson et al., 2014)

EBV\BZLF1^GMR.PA has eye phenotype, enhanceable by Tor[+]/mTor^k17004

(Adamson et al., 2014)

EBV\BZLF1^GMR.PA has eye phenotype, enhanceable by Rab5^KG05684

(Adamson and Lajeunesse, 2012)

EBV\BZLF1^GMR.PA has eye phenotype, enhanceable by ago^EY01092

(Adamson and Lajeunesse, 2012)

EBV\BZLF1^GMR.PA has eye phenotype, enhanceable by dlg1^G0276

(Adamson and Lajeunesse, 2012)

EBV\BZLF1^GMR.PA has eye phenotype, enhanceable by p53^GMR.Ex

(Adamson and Lajeunesse, 2012)

EBV\BZLF1^GMR.PA has eye phenotype, enhanceable by rpr^GMR.PH

(Adamson and Lajeunesse, 2012)

EBV\BZLF1^GMR.PA has eye phenotype, enhanceable by Ras85D^e1B

(Adamson and Lajeunesse, 2012)

EBV\BZLF1^GMR.PA has eye phenotype, enhanceable by mTor^ΔP

(Adamson and Lajeunesse, 2012)

EBV\BZLF1^GMR.PA has eye phenotype, enhanceable by mTor^k17004

(Adamson and Lajeunesse, 2012)

EBV\BZLF1^GMR.PA has eye phenotype, enhanceable by sv^spa-pol/sv[+]

(Adamson et al., 2005)

NOT Enhanced by

Statement

Reference

EBV\BZLF1^GMR.PA has eye phenotype, non-enhanceable by BacA\p35^GMR.PH

(Adamson et al., 2005)

EBV\BZLF1^GMR.PA has eye phenotype, non-enhanceable by CycE^AR95

(Adamson et al., 2005)

EBV\BZLF1^GMR.PA has eye phenotype, non-enhanceable by Rbf^GMR.PD

(Adamson et al., 2005)

EBV\BZLF1^GMR.PA has eye phenotype, non-enhanceable by dap^GMR.PdN

(Adamson et al., 2005)

Suppressed by

Statement

Reference

EBV\BZLF1^GMR.PA has eye phenotype, suppressible by hyd¹⁵

(Adamson and Lajeunesse, 2012)

EBV\BZLF1^GMR.PA has eye phenotype, suppressible by l(2)gd1^EY04750

(Adamson and Lajeunesse, 2012)

EBV\BZLF1^GMR.PA has eye phenotype, suppressible by 14-3-3ε^S-696

(Adamson and Lajeunesse, 2012)

NOT suppressed by

Statement

Reference

EBV\BZLF1^GMR.PA has eye phenotype, non-suppressible by BacA\p35^GMR.PH

(Adamson et al., 2005)

EBV\BZLF1^GMR.PA has eye phenotype, non-suppressible by CycE^AR95

(Adamson et al., 2005)

EBV\BZLF1^GMR.PA has eye phenotype, non-suppressible by Rbf^GMR.PD

(Adamson et al., 2005)

EBV\BZLF1^GMR.PA has eye phenotype, non-suppressible by dap^GMR.PdN

(Adamson et al., 2005)

Other

Statement

Reference

EBV\BRLF1^GMR.PA, EBV\BZLF1^GMR.PA has eye phenotype

(Adamson and Lajeunesse, 2012)

EBV\BRLF1^GMR.PA, EBV\BZLF1^GMR.PA has ommatidium phenotype

(Adamson and Lajeunesse, 2012)

EBV\BRLF1^GMR.PA, EBV\BZLF1^GMR.PA has interommatidial bristle phenotype

(Adamson and Lajeunesse, 2012)

EBV\BRLF1^GMR.PA, EBV\BZLF1^GMR.PA has eye disc | third instar larval stage phenotype

(Adamson and Lajeunesse, 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Tor^ΔP/+ partially enhances the eye-phenotype associated with EBV\BRLF1^GMR.PA-expression.

Tor^k17004/+ partially enhances the eye-phenotype associated with EBV\BRLF1^GMR.PA-expression.

(Adamson et al., 2014)

While EBV\BZLF1^GMR.PA expression alone does not significantly alter the number of mitotic cells in eye discs, expression of EBV\BZLF1^GMR.PA reverses the EBV\BRLF1^GMR.PA overproliferation, such that the number of mitotic cells is lower than in wild-type controls. The number of short hair-like bristles in the adult eye is also decreased. The eye color of the double-transgenic flies is very similar to that of single-transgenic EBV\BZLF1^GMR.PA flies (orange), suggesting that pigment cells do not over-proliferate as in EBV\BRLF1^GMR.PA-eyes. The double-transgenic flies show a complete loss of ommatidia, although the bristles remain.

(Adamson and Lajeunesse, 2012)

The eye phenotype of flies carrying a weakly expressing P{GMR-BZLF1.A} line is unaffected by dap^GMR.PdN, Rbf^GMR.PD, CycE^AR95 or BacA\p35^GMR.PH. The eye phenotype of flies carrying a weakly expressing P{GMR-BZLF1.A} line is enhanced by sv^spa-pol/+.

(Adamson et al., 2005)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

EBV\BZLF1^GMR.PA

Name Synonyms

glass multimer reporter construct of Adamson

(Adamson et al., 2005)

Secondary FlyBase IDs

References (4)

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