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General Information
Symbol
EBV\BZLF1GMR.PA
Species
L. Cercopithecine herpesvirus 15
Name
glass multimer reporter construct of Adamson
FlyBase ID
FBal0190744
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

GMR regulatory sequences drive expression of a EBV\BZLF1 cDNA.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of EBV\BZLF1GMR.PA results in dose-dependent eye phenotypes including rough eye, diminished pigmentation, and loss of ommatidia.

Flies carrying EBV\BZLF1GMR.PA show loss of all eye structures except the interommatidial bristles, which are disorganised and scattered throughout the thin cuticle that remains in place of the eye. EBV\BZLF1GMR.PA third larval instar eye discs show a significantly smaller number of cells which incorporate BrdU compared to wild-type discs and there is an increase in cell death in the discs compared to wild type. Flies carrying a weakly expressing P{GMR-BZLF1.A} line show a dose sensitive eye phenotype, with flies carrying one copy having a moderate eye phenotype with some eye tissue (but no recognisable ommatidia) and flies having two copies lacking all eye structures except the interommatidial bristles.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference

EBV\BZLF1GMR.PA has visible phenotype, enhanceable by svspa-pol/sv[+]/sv+

NOT Enhanced by
Statement
Reference

EBV\BZLF1GMR.PA has visible phenotype, non-enhanceable by dapGMR.PdN

EBV\BZLF1GMR.PA has visible phenotype, non-enhanceable by BacA\p35GMR.PH

EBV\BZLF1GMR.PA has visible phenotype, non-enhanceable by CycEAR95

EBV\BZLF1GMR.PA has visible phenotype, non-enhanceable by RbfGMR.PD

NOT suppressed by
Statement
Reference

EBV\BZLF1GMR.PA has visible phenotype, non-suppressible by BacA\p35GMR.PH

EBV\BZLF1GMR.PA has visible phenotype, non-suppressible by dapGMR.PdN

EBV\BZLF1GMR.PA has visible phenotype, non-suppressible by CycEAR95

EBV\BZLF1GMR.PA has visible phenotype, non-suppressible by RbfGMR.PD

Suppressor of
Other
Phenotype Manifest In
Enhanced by
Statement
Reference

EBV\BZLF1GMR.PA has eye phenotype, enhanceable by Tor[+]/TorΔP

EBV\BZLF1GMR.PA has eye phenotype, enhanceable by Tor[+]/Tork17004

EBV\BZLF1GMR.PA has eye phenotype, enhanceable by svspa-pol/sv[+]/sv+

NOT Enhanced by
Statement
Reference

EBV\BZLF1GMR.PA has eye phenotype, non-enhanceable by BacA\p35GMR.PH

EBV\BZLF1GMR.PA has eye phenotype, non-enhanceable by CycEAR95

EBV\BZLF1GMR.PA has eye phenotype, non-enhanceable by RbfGMR.PD

EBV\BZLF1GMR.PA has eye phenotype, non-enhanceable by dapGMR.PdN

Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference

EBV\BZLF1GMR.PA has eye phenotype, non-suppressible by BacA\p35GMR.PH

EBV\BZLF1GMR.PA has eye phenotype, non-suppressible by CycEAR95

EBV\BZLF1GMR.PA has eye phenotype, non-suppressible by RbfGMR.PD

EBV\BZLF1GMR.PA has eye phenotype, non-suppressible by dapGMR.PdN

Other
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference

TorΔP/+ partially enhances the eye-phenotype associated with EBV\BRLF1GMR.PA-expression.

Tork17004/+ partially enhances the eye-phenotype associated with EBV\BRLF1GMR.PA-expression.

While EBV\BZLF1GMR.PA expression alone does not significantly alter the number of mitotic cells in eye discs, expression of EBV\BZLF1GMR.PA reverses the EBV\BRLF1GMR.PA overproliferation, such that the number of mitotic cells is lower than in wild-type controls. The number of short hair-like bristles in the adult eye is also decreased. The eye color of the double-transgenic flies is very similar to that of single-transgenic EBV\BZLF1GMR.PA flies (orange), suggesting that pigment cells do not over-proliferate as in EBV\BRLF1GMR.PA-eyes. The double-transgenic flies show a complete loss of ommatidia, although the bristles remain.

The eye phenotype of flies carrying a weakly expressing P{GMR-BZLF1.A} line is unaffected by dapGMR.PdN, RbfGMR.PD, CycEAR95 or BacA\p35GMR.PH. The eye phenotype of flies carrying a weakly expressing P{GMR-BZLF1.A} line is enhanced by svspa-pol/+.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
EBV\BZLF1GMR.PA
Name Synonyms
glass multimer reporter construct of Adamson
Secondary FlyBase IDs
    References (3)