FlyBase Allele Report: Dmel\RhoGEF2[UASp.Tag:T7]

General Information

Symbol

Dmel\RhoGEF2^UASp.Tag:T7

Species

D. melanogaster

Name

FlyBase ID

FBal0190772

Feature type

allele

Associated gene

Dmel\RhoGEF2

Associated Insertion(s)

Carried in Construct

P{UASp-T7.RhoGEF2}

Also Known As

UAS-RhoGEF2, RhoGEF2-OE

Key Links

Transgenic product class

wild_type

(Barrett, 2005.12.15)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Barrett, 2005.12.15)

Mutagen

in vitro construct

(Barrett, 2005.12.15)

Progenitor genotype

Carried in construct

P{UASp-T7.RhoGEF2}

Cytology

Description

Expression of N-terminal Tag:T7 tagged version of RhoGEF2 is governed by UASp regulatory sequences.

(Barrett, 2005.12.15)

Allele components

Component

Use(s)

Regulatory region(s)

UASp

binary expression system - regulatory region

Encoded product / tool

RhoGEF2

Tagged with

Tag:T7

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Embryos expressing RhoGEF2^UASp.Tag:T7 under the control of Scer\GAL4^VP16.nos.UTR show enhanced contractile ring constriction in mid and late cellularization stages, with decreased furrow length.

(Sharma and Rikhy, 2021)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

RhoGEF2^UASp.Tag:T7, Scer\GAL4^GMR.PU is a suppressor of abnormal cell shape | adult stage phenotype of RhoGAP71E^UAS.GFP, Scer\GAL4^GMR.PU

(Rosa-Birriel et al., 2024)

Phenotype Manifest In

Suppressed by

Statement

Reference

RhoGEF2^UASp.Tag:T7, Scer\GAL4^{VP16.nanos.UTR} has actomyosin contractile ring | maternal effect | embryonic stage 5 phenotype, suppressible by Graf^EY09461, Scer\GAL4^{VP16.nanos.UTR}

(Sharma and Rikhy, 2021)

Suppressor of

Statement

Reference

RhoGEF2^UASp.Tag:T7, Scer\GAL4^GMR.PU is a suppressor of eye phenotype of RhoGAP71E^UAS.GFP, Scer\GAL4^GMR.PU

(Rosa-Birriel et al., 2024)

RhoGEF2^UASp.Tag:T7, Scer\GAL4^GMR.PU is a suppressor of pigment cell phenotype of RhoGAP71E^UAS.GFP, Scer\GAL4^GMR.PU

(Rosa-Birriel et al., 2024)

RhoGEF2^UASp.Tag:T7, Scer\GAL4^{VP16.nanos.UTR} is a suppressor of actomyosin contractile ring | maternal effect | embryonic stage 5 phenotype of Mbs^GL01207, Scer\GAL4^{VP16.nanos.UTR}

(Sharma and Rikhy, 2021)

RhoGEF2^UASp.Tag:T7, Scer\GAL4^{VP16.nanos.UTR} is a suppressor of actomyosin contractile ring | maternal effect | embryonic stage 5 phenotype of Graf^EY09461, Scer\GAL4^{VP16.nanos.UTR}

(Sharma and Rikhy, 2021)

Other

Statement

Reference

RhoGAP71E^{UAS.ORF.Tag:HA}, RhoGEF2^UASp.Tag:T7, Scer\GAL4^{VP16.mat.αTub67C} has actomyosin | embryonic stage phenotype

(Jackson et al., 2024)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

9387

y¹ w^*; P{UASp-T7.RhoGEF2}6b

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

RhoGEF2^{Scer\UAS.P\T.T:Uuuu\T7}

RhoGEF2^UASp.Tag:T7

Name Synonyms

Secondary FlyBase IDs

References (11)

Report Sections

Open Close