Allele Dmel\desat1^1573-1

General Information
Symbol	Dmel\desat1^1573-1	Species	D. melanogaster
Name		FlyBase ID	FBal0191020
Feature type	allele	Associated gene	Dmel\desat1

Allele class
Mutagen
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference P{GawB} insertion in the first intron of desat1. (Marcillac et al., 2005) Insertion at -1691bp relative to the ATG site. (Marcillac et al., 2005)
Caused by insertion	P{GawB}desat1^1573-1 (Marcillac et al., 2005, Houot et al., 2012, Marcillac and Ferveur, 2004, Marcillac et al., 2005)
Cytology
Phenotypic Data
Phenotypic Class
	courtship behavior defective (Houot et al., 2012) fertile (Marcillac et al., 2005) mating behavior defective \| male limited (Li et al., 2011) viable (Marcillac et al., 2005, Marcillac et al., 2005)
Phenotype Manifest In

Detailed Description
	Statement Reference Mutant desat1[1573-1] males show no significant difference in their courtship of males compared to females, indicating a loss of sex discrimination. Mutant desat1[1573-1] males selected for their ability to discriminate between the sexes over 10 generations exhibit a preference for wild-type females over desat1[1573-1] mutant females. Mutant desat1[1573-1] males selected for their inability to discriminate between the sexes over 10 generations exhibit a preference for desat1[1573-1] mutant females over wild-type females. In contrast, the relatively high discrimination of male pheromones shown by unselected desat1[1573-1] males disappears in lines selected for either the ability or inability to discriminate between the sexes. (Houot et al., 2012) Mutant males show increased male-male courtship in red light. (Li et al., 2011) Mutant males show an increase in the total amount of cuticular hydrocarbons compared to control males. They show a significant decrease in the relative proportion of desaturated cuticular hydrocarbons and a significant increase in the relative proportion of linear cuticular hydrocarbons compared to control males. Mutant females show an increase in the total amount of cuticular hydrocarbons compared to control females. They show a significant decrease in the relative proportion of desaturated cuticular hydrocarbons and a significant increase in the relative proportion of linear cuticular hydrocarbons compared to control females. (Houot et al., 2010) Homozygous flies of both sexes have defective cuticular hydrocarbon (CH) profiles. Both sexes produce substantially smaller amounts of unsaturated CHs with one and two double bonds (monoenes and dienes); for example, in homozygous females, 7,11-dienes represent only 2.6% of the total CH profile, compared to 37.8% in control females, while in homozygous males, 7-monoenes represent only 5.6% of the total CH profile, compared to 52.4% in control males. Homozygous flies of both sexes have much higher levels of n-alkanes than control flies, with n-alkanes representing 60.5% of the total CH profile in females (compared to 11.1% in controls) and 67.7% of the total CH profile in males (compared to 17% in controls). The overall amount of CHs is highly increased in homozygous males (+176%) and females (+48%) compared to same-sex control flies. This increase is mainly caused by linear alkanes, not methyl-alkanes. Heterozygous mutant flies show only a moderate variation in the cuticular hydrocarbon (CH) profile ; the total amount of CHs is similar to that of controls, the percentage of alkenes is decreased by 20-30% and the percentage of alkanes is increased by the same proportion. (Marcillac et al., 2005) Mutant males and females have radically decreased proportions of unsaturated hydrocarbons on their cuticle compared to wild type. This includes a reduction in the main female pheromone (7,11-dienes) in the mutant females and the main male pheromone (7-tricosene) in the mutant males. The mutants have a higher proportion of saturated hydrocarbons on their cuticle compared to wild type, particularly n-pentacosane in mutant females and n-tricosane in mutant males. Under red light conditions (in which flies are effectively blind), wild-type males indiscriminately court male and female mutant flies (whereas they clearly prefer courting female flies when presented with male and female control flies). Under white light conditions, mutant males are able to discriminate between wild-type male and female flies, preferring to court with females. However, under red light conditions, the mutant males indiscriminately court wild-type male and female flies. (Marcillac et al., 2005)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference desat1^1573-1 has courtship behavior defective phenotype, enhanceable by qtc^d00941 (Houot et al., 2012)
Suppressed by
Statement Reference desat1^1573-1 has courtship behavior defective phenotype, suppressible by Sh^MB02366 (Houot et al., 2012)
Enhancer of
Statement Reference desat1^1573-1 is an enhancer of courtship behavior defective phenotype of qtc^d00941 (Houot et al., 2012)
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference A Sh[MB02366] mutant background restores the ability to discriminate between the sexes in desat1[1573-1] mutants. Double mutant desat1[1573-1] qtc[d00941] males show significant sex discrimination ability, but in the opposite direction to normal discrimination: they spend more time courting target males than target females, and their courtship of females is much lower than in controls. These males show a decreased response to wild-type female pheromones and increased response to wild-type male pheromones. (Houot et al., 2012) The level of homosexual courtship of gb^KG07905 males is reduced to wild-type levels when they are partnered with desat1^1573-1 males instead of with wild-type males. (Grosjean et al., 2008)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 4 )
Reported As
Symbol Synonym	1573-1 (Houot et al., 2010) desat1^1-1573 (Grosjean et al., 2008) desat1^1573-1 (Marcillac et al., 2005, Marcillac and Ferveur, 2004) desat1¹⁵⁷³ (Li et al., 2011)
Name Synonym
Secondary FlyBase IDs

References ( 7 )
Research paper	Houot et al., 2012, PLoS ONE 7(1): e30799 Genes Involved in Sex Pheromone Discrimination in Drosophila melanogaster and Their Background-Dependent Effect. [FBrf0217343] Li et al., 2011, PLoS ONE 6(11): e28269 The hector G-Protein Coupled Receptor Is Required in a Subset of fruitless Neurons for Male Courtship Behavior. [FBrf0216834] Houot et al., 2010, Genetics 185(4): 1297--1309 The Consequences of Regulation of desat1 Expression for Pheromone Emission and Detection in Drosophila melanogaster. [FBrf0211531] Grosjean et al., 2008, Nat. Neurosci. 11(1): 54--61 A glial amino-acid transporter controls synapse strength and courtship in Drosophila. [FBrf0201004] Marcillac et al., 2005, Proc. Biol. Sci. 272(1560): 303--309 A single mutation alters production and discrimination of Drosophila sex pheromones. [FBrf0184242] Marcillac et al., 2005, Genetics 171(4): 1617--1628 A mutation with major effects on Drosophila melanogaster sex pheromones. [FBrf0190745] Marcillac and Ferveur, 2004, J. Exp. Biol. 207(Pt 22): 3927--3933 A set of female pheromones affects reproduction before, during and after mating in Drosophila. [FBrf0180451]

Allele Dmel\desat11573-1

Allele Dmel\desat1^1573-1