Allele Dmel\park^{Scer\UAS.T:Hsap\MYC}

General Information
Symbol	Dmel\park^{Scer\UAS.T:Hsap\MYC}	Species	D. melanogaster
Name		FlyBase ID	FBal0191121
Feature type	allele	Associated gene	Dmel\park

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - coding region fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - coding region fusion (Cha et al., 2005) in vitro construct - regulatory fusion (Cha et al., 2005)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS regulatory sequences drive expression of a wild-type full-length park cDNA with a T:Hsap\MYC tag downstream. (Cha et al., 2005)
Carried in construct	P{UAS-park-Myc} (Cha et al., 2005, Park et al., 2006, Park et al., 2009, Kim et al., 2008)
Tagged with	T:Hsap\MYC (Cha et al., 2005)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In
	adult thorax, with Scer\GAL4^ap-md544 (Cha et al., 2005) mesothoracic cleft \| ectopic, with Scer\GAL4^ap-md544 (Cha et al., 2005)
Detailed Description
	Statement Reference Animals expressing park[Scer\UAS.T:Hsap\MYC] under the control of Scer\GAL4[Mef2.PR] results in mitochondria of almost normal size in the adult thorax. (Park et al., 2009) Expression of park^{Scer\UAS.T:Hsap\MYC} in the notum of wing imaginal discs (under the regulation of Scer\GAL4^ap-md544) induces a marked cleft in the thorax, in a park-dose-dependent manner. (Cha et al., 2005)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement Reference park^{Scer\UAS.T:Hsap\MYC}/Scer\GAL4^hs.PB is a suppressor \| partially of flightless phenotype of Pink1^B9 (Park et al., 2006) park^{Scer\UAS.T:Hsap\MYC}/Scer\GAL4^hs.PB is a suppressor of locomotor behavior defective phenotype of Pink1^B9 (Park et al., 2006)
Other
Statement Reference Pink1^{Scer\UAS.T:Ivir\HA1}, Scer\GAL4^hs.PB, park^{Scer\UAS.T:Hsap\MYC} has lethal \| pupal stage \| heat sensitive phenotype (Park et al., 2009)
Phenotype Manifest In
Enhanced by
Statement Reference Scer\GAL4^ap-md544, park^{Scer\UAS.T:Hsap\MYC} has adult thorax phenotype, enhanceable by hep[+]/hep¹ (Cha et al., 2005) Scer\GAL4^ap-md544, park^{Scer\UAS.T:Hsap\MYC} has adult thorax phenotype, enhanceable by Pk92B^{K618M.Scer\UAS}, Scer\GAL4^ap-md544 (Cha et al., 2005) Scer\GAL4^ap-md544, park^{Scer\UAS.T:Hsap\MYC} has adult thorax phenotype, enhanceable by Scer\GAL4^ap-md544/bsk^{K53R.Scer\UAS} (Cha et al., 2005) Scer\GAL4^ap-md544, park^{Scer\UAS.T:Hsap\MYC} has adult thorax phenotype, enhanceable by Scer\GAL4^ap-md544/puc^Scer\UAS.cMa (Cha et al., 2005) Scer\GAL4^ap-md544, park^{Scer\UAS.T:Hsap\MYC} has mesothoracic cleft \| ectopic phenotype, enhanceable by hep[+]/hep¹ (Cha et al., 2005) Scer\GAL4^ap-md544, park^{Scer\UAS.T:Hsap\MYC} has mesothoracic cleft \| ectopic phenotype, enhanceable by Pk92B^{K618M.Scer\UAS}, Scer\GAL4^ap-md544 (Cha et al., 2005) Scer\GAL4^ap-md544, park^{Scer\UAS.T:Hsap\MYC} has mesothoracic cleft \| ectopic phenotype, enhanceable by Scer\GAL4^ap-md544/bsk^{K53R.Scer\UAS} (Cha et al., 2005) Scer\GAL4^ap-md544, park^{Scer\UAS.T:Hsap\MYC} has mesothoracic cleft \| ectopic phenotype, enhanceable by Scer\GAL4^ap-md544/puc^Scer\UAS.cMa (Cha et al., 2005)
Suppressed by
Statement Reference Scer\GAL4^ap-md544, park^{Scer\UAS.T:Hsap\MYC} has adult thorax phenotype, suppressible \| partially by Pk92B^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^ap-md544 (Cha et al., 2005) Scer\GAL4^ap-md544, park^{Scer\UAS.T:Hsap\MYC} has adult thorax phenotype, suppressible by GIIIspla2^EP1516 (Cha et al., 2005) Scer\GAL4^ap-md544, park^{Scer\UAS.T:Hsap\MYC} has adult thorax phenotype, suppressible by Scer\GAL4^ap-md544/bsk^Scer\UAS.cBa (Cha et al., 2005) Scer\GAL4^ap-md544, park^{Scer\UAS.T:Hsap\MYC} has adult thorax phenotype, suppressible by Scer\GAL4^ap-md544/hep^Act.Scer\UAS (Cha et al., 2005) Scer\GAL4^ap-md544, park^{Scer\UAS.T:Hsap\MYC} has adult thorax phenotype, suppressible by Traf4^EP578 (Cha et al., 2005) Scer\GAL4^ap-md544, park^{Scer\UAS.T:Hsap\MYC} has mesothoracic cleft \| ectopic phenotype, suppressible by GIIIspla2^EP1516 (Cha et al., 2005) Scer\GAL4^ap-md544, park^{Scer\UAS.T:Hsap\MYC} has mesothoracic cleft \| ectopic phenotype, suppressible by Pk92B^{Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^ap-md544 (Cha et al., 2005) Scer\GAL4^ap-md544, park^{Scer\UAS.T:Hsap\MYC} has mesothoracic cleft \| ectopic phenotype, suppressible by Scer\GAL4^ap-md544/bsk^Scer\UAS.cBa (Cha et al., 2005) Scer\GAL4^ap-md544, park^{Scer\UAS.T:Hsap\MYC} has mesothoracic cleft \| ectopic phenotype, suppressible by Traf4^EP578/Scer\GAL4^ap-md544 (Cha et al., 2005)
Enhancer of
Statement Reference Scer\GAL4^ap-md544, park[+], park^{Scer\UAS.T:Hsap\MYC} is an enhancer of adult thorax phenotype of Scer\GAL4^ap-md544, bsk^{K53R.Scer\UAS} (Cha et al., 2005) Scer\GAL4^ap-md544, park[+], park^{Scer\UAS.T:Hsap\MYC} is an enhancer of adult thorax phenotype of Scer\GAL4^ap-md544, puc^Scer\UAS.cMa (Cha et al., 2005) Scer\GAL4^ap-md544, park[+], park^{Scer\UAS.T:Hsap\MYC} is an enhancer of mesothoracic cleft \| ectopic phenotype of Scer\GAL4^ap-md544, bsk^{K53R.Scer\UAS} (Cha et al., 2005) Scer\GAL4^ap-md544, park[+], park^{Scer\UAS.T:Hsap\MYC} is an enhancer of mesothoracic cleft \| ectopic phenotype of Scer\GAL4^ap-md544, puc^Scer\UAS.cMa (Cha et al., 2005)
Suppressor of
Statement Reference park^{Scer\UAS.T:Hsap\MYC}/Scer\GAL4^hs.PB is a suppressor \| partially of adult thorax phenotype of Pink1^B9 (Park et al., 2006) park^{Scer\UAS.T:Hsap\MYC}/Scer\GAL4^hs.PB is a suppressor \| partially of dopamine neuron & adult brain phenotype of Pink1^B9 (Park et al., 2006) park^{Scer\UAS.T:Hsap\MYC}/Scer\GAL4^hs.PB is a suppressor \| partially of mitochondrion phenotype of Pink1^B9, park¹ (Kim et al., 2008) park^{Scer\UAS.T:Hsap\MYC}/Scer\GAL4^hs.PB is a suppressor of indirect flight muscle phenotype of Pink1^B9 (Park et al., 2006) park^{Scer\UAS.T:Hsap\MYC}/Scer\GAL4^hs.PB is a suppressor of wing phenotype of Pink1^B9 (Park et al., 2006) park^{Scer\UAS.T:Hsap\MYC}/Scer\GAL4^ple.PF is a suppressor \| partially of mitochondrion & dopamine neuron & adult brain phenotype of Pink1^B9 (Park et al., 2006) park^{Scer\UAS.T:Hsap\MYC}/Scer\GAL4^ple.PF is a suppressor of mitochondrion & dopaminergic neuron phenotype of Pink1^B9 (Park et al., 2009) Scer\GAL4^ap-md544, park[+], park^{Scer\UAS.T:Hsap\MYC} is a suppressor of mesothoracic cleft \| ectopic phenotype of Scer\GAL4^ap-md544, Traf4^EP578 (Cha et al., 2005)
Other
Statement Reference Pink1^{Scer\UAS.T:Ivir\HA1}, Scer\GAL4^hs.PB, park^{Scer\UAS.T:Hsap\MYC} has mitochondrion & indirect flight muscle phenotype (Park et al., 2009)
Additional Comments
Genetic Interactions
Statement Reference Co-expression of Pink1[Scer\UAS.T:Ivir\HA1] and park[Scer\UAS.T:Hsap\MYC] under the control of Scer\GAL4[hs.PB] at 25[o]C results in pupal lethality, but at 18[o]C, several adult escapers are obtained. These escapers have smaller mitochondria in the indirect flight muscles compared to controls. Co-expression of park[Scer\UAS.T:Hsap\MYC] in adults expressing opa1-like[Scer\UAS.T:Zzzz\FLAG] under the control of Scer\GAL4[Mef2.PR] results in smaller than normal mitochondria in the adult thorax. The swollen mitochondria phenotype seen in dopaminergic neurons of Pink1[B9] mutants is suppressed by expression of park[Scer\UAS.T:Hsap\MYC] under the control of Scer\GAL4[ple.PF]. (Park et al., 2009) Expression of park[Scer\UAS.T:Hsap\MYC] under the control of Scer\GAL4[hs.PB] partially suppresses the mitochondrial swelling phenotype in Pink1[B9], park[1] double mutants. (Kim et al., 2008) Expression of park^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^hs.PB markedly suppresses the downturned wing and crushed thorax phenotypes seen in Pink1^B9 flies. The indirect flight muscle defects are also suppressed in these animals. The loss of dopaminergic neurons in the adult brain and the enlarged mitochondria phenotype seen in the remaining dopaminergic neurons is also significantly suppressed. The rescued flies show increased climbing speed and have some flight ability. (Park et al., 2006) The thoracic cleft formation observed in park^{Scer\UAS.T:Hsap\MYC} overexpression mutants (Scer\GAL4^ap-md544) and bsk^{K53R.Scer\UAS} mutants is mutually enhanced by their co-expression. The thoracic cleft formation observed in park^{Scer\UAS.T:Hsap\MYC} overexpression mutants (Scer\GAL4^ap-md544) is suppressed by co-expression of bsk^Scer\UAS.cBa. Overexpression of puc^Scer\UAS.cMa and park^{Scer\UAS.T:Hsap\MYC} in the notum of the wing imaginal discs, under the regulation of Scer\GAL4^ap-md544 enhances the thoracic cleft observed in each single overexpression. Expression of park^{Scer\UAS.T:Hsap\MYC} in the notum of the wing imaginal discs, under the regulation of Scer\GAL4^ap-md544, in hep¹/+ mutants enhances the thoracic cleft phenotype observed in park^{Scer\UAS.T:Hsap\MYC}/Scer\GAL4^ap-md544 mutants alone. Co-expression of hep^Act.Scer\UAS, with park^{Scer\UAS.T:Hsap\MYC}, in the notum of the wing imaginal discs, under the regulation of Scer\GAL4^ap-md544 suppresses the park^{Scer\UAS.T:Hsap\MYC} phenotype of thoracic cleft formation resulting from abnormalities in JNK signalling during thorax closure. The thoracic cleft formation observed in park^{Scer\UAS.T:Hsap\MYC} overexpression mutants (Scer\GAL4^ap-md544) is suppressed by co-expression of Traf1^EP578. Overexpression of puc^Scer\UAS.cMa and park^{Scer\UAS.T:Hsap\MYC} in the notum of wing imaginal discs, under the regulation of Scer\GAL4^ap-md544 enhances the thoracic cleft observed in each single overexpression. Overexpression of Pk92B^{Scer\UAS.T:Zzzz\FLAG} and park^{Scer\UAS.T:Hsap\MYC} in the notum of wing imaginal discs, under the regulation of Scer\GAL4^ap-md544 partially suppresses the thoracic cleft observed in park^{Scer\UAS.T:Hsap\MYC} mutants. The thoracic cleft formation observed in park^{Scer\UAS.T:Hsap\MYC} overexpression mutants (Scer\GAL4^ap-md544) is enhanced by co-expression of Pk92B^{K618M.Scer\UAS}. (Cha et al., 2005) Co-expression of Traf2^EP1516, with park^{Scer\UAS.T:Hsap\MYC}, in the notum of the wing imaginal discs, under the regulation of Scer\GAL4^ap-md544 suppresses the park^{Scer\UAS.T:Hsap\MYC} phenotype of thoracic cleft formation resulting from abnormalities in JNK signalling during thorax closure. (Cha et al., 2005)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	park^{Scer\UAS.T:Hsap\MYC}/Scer\GAL4^Ddc.PL rescues park¹ (Cha et al., 2005) park^{Scer\UAS.T:Hsap\MYC} rescues park¹ (Kim et al., 2008)
Comments	The mitochondrial morphology in the indirect flight muscle of park[1] mutants is fully rescued by expression of park[Scer\UAS.T:Hsap\MYC] under the control of Scer\GAL4[hs.PB] (Kim et al., 2008) The park¹ dorsomedial dopaminergic neuron morphology and loss phenotype can be completely rescued by the expression of park^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Ddc.PL. (Cha et al., 2005)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	park^{Scer\UAS.T:Hsap\MYC}
Name Synonym
Secondary FlyBase IDs

References ( 4 )
Research paper	Park et al., 2009, Biochem. Biophys. Res. Commun. 378(3): 518--523 The PINK1-Parkin pathway is involved in the regulation of mitochondrial remodeling process. [FBrf0207317] Kim et al., 2008, Biochem. Biophys. Res. Commun. 377(3): 975--980 PINK1 controls mitochondrial localization of Parkin through direct phosphorylation. [FBrf0207333] Park et al., 2006, Nature 441(7097): 1157--1161 Mitochondrial dysfunction in Drosophila PINK1 mutants is complemented by parkin. [FBrf0193630] Cha et al., 2005, Proc. Natl. Acad. Sci. U.S.A. 102(29): 10345--10350 Parkin negatively regulates JNK pathway in the dopaminergic neurons of Drosophila. [FBrf0188253]

Allele Dmel\parkScer\UAS.T:Hsap\MYC

Allele Dmel\park^{Scer\UAS.T:Hsap\MYC}