Allele Dmel\mir-1^KO

General Information
Symbol	Dmel\mir-1^KO	Species	D. melanogaster
Name		FlyBase ID	FBal0191185
Feature type	allele	Associated gene	Dmel\mir-1

Allele class
Mutagen	FLPase, SCEI endonuclease, recombination, I-CreI endonuclease, ends-in gene targeting
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	ends-in gene targeting (Sokol and Ambros, 2005) FLPase (Sokol and Ambros, 2005) I-CreI endonuclease (Sokol and Ambros, 2005) recombination (Sokol and Ambros, 2005) SCEI endonuclease (Sokol and Ambros, 2005)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	mir-1^{KO.Scer\SceI.RS} (Sokol and Ambros, 2005)
Nature of the lesion	Statement Reference Ends-in homologous targeting event using mir-1^{KO.Scer\SceI.RS} as the donor. The expected target locus duplication produced by the first homologous recombination event has been reduced to a single copy using Crei\I-CreI recombinase. This results in the replacement of 57bp of the mir-1 locus, including the entire mir-1 21mer, with a XhoI site. (Sokol and Ambros, 2005)
Cytology
Phenotypic Data
Phenotypic Class
	hypoactive \| larval stage \| recessive (Sokol and Ambros, 2005) lethal \| recessive \| second instar larval stage (Sokol and Ambros, 2005)
Phenotype Manifest In
	embryonic/larval somatic muscle \| second instar larval stage (Sokol and Ambros, 2005)
Detailed Description
	Statement Reference Mutants show no defects in targeting of the DL1 glomerulus in the antennal lobe. (Berdnik et al., 2008) Most homozygotes die as small second instar larvae 2-7 days after hatching. These larvae never grow larger than the size they are as young second instars, they become increasingly lethargic and compromised in their movement and prior to death they stop moving entirely (their only sign of life is responsiveness to touch and contractions of the dorsal vessel). In addition, the bodies of the larvae appear to collapse and flatten over time. The organisation and morphology of the body wall musculature of mutant first instar larvae appears normal, but in mutant second instar larvae the body wall musculature is severely disrupted. The muscle function of newly hatched homozygous larvae is essentially normal; body wall contraction rates are unaffected, and dorsal vessel contraction rates and behaviour are almost indistinguishable from wild type. However, only 15% of newly hatched mutant larvae score positive for solid food ingestion within 30 minutes (in contrast to 100% of wild-type controls). All mutant larvae score positive for solid food ingestion by 7 hours after being exposed to food. Mutant larval excretion rates are normal. Newly hatched mutant larvae ingest liquid food at the same rate as wild-type controls. Mutant first instar larvae that are fed on sucrose show the same foraging behaviour and longevity as wild-type larvae fed on sucrose, arresting development as first instars and continuing to crawl incessantly and at normal rates long after age matched mutant larvae fed on solid food have become immobilised and died. The body wall contraction rates of mutant first instar larvae fed on sucrose are maintained at nearly wild-type levels. Homozygous embryos derived from homozygous germline clones (lacking both maternal and zygotic mir-1 function) have an identical phenotype to homozygous embryos (lacking zygotic mir-1 function), and do not show any defects in the differentiation or development of the embryonic mesoderm or muscle system. (Sokol and Ambros, 2005)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	mir-1^KO is rescued by mir-1^+t8.6 (Sokol and Ambros, 2005) mir-1^KO is rescued by Scer\GAL4^how-24B/mir-1^{Scer\UAS.P\T.cSa}/Scer\GAL4^how-24B (Sokol and Ambros, 2005)
Not rescued by	mir-1^KO is not rescued by mir-1^8.6.XhoI (Sokol and Ambros, 2005)
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	DmiR-1^KO (Sokol and Ambros, 2005) mir-1^KO
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Sokol and Ambros, 2005, Genes Dev. 19(19): 2343--2354 Mesodermally expressed Drosophila microRNA-1 is regulated by Twist and is required in muscles during larval growth. [FBrf0190563]
Supplementary material	Berdnik et al., 2008, Curr. Biol. 18(22): Supplemental Data. [FBrf0210620]

Allele Dmel\mir-1KO

Allele Dmel\mir-1^KO