Overexpression of
hpoScer\UAS.cUa in the GMR domain of the developing eye (under the control of
Scer\GAL4GMR.PF) induces massive cell death without disrupting cell differentiation or patterning. In mid-pupal retina (around 24 hours after pupal formation), both photoreceptor and pigment cells continue to die precociously. In comparison, in wild-type pupal retinae cell death occurs prominently in the pigment cells, and around the retinal boundary. Compared with wild-type adult eyes,
hpoScer\UAS.cUa overexpressing adult flies are arrested as pharates at room temperature, and at 18[o]C show small rough eyes.
hpoScer\UAS.cUa overexpression causes apoptosis in all epithelial tissues, for example, in the wing disc short pulses of
hpoScer\UAS.cUa expression using heat shock (
Scer\GAL4hs.PB) causes apoptosis. In comparison, very few cells undergo apoptosis in wild-type wing discs.
Overexpression of
hpoScer\UAS.cUa in the eye, under the control of
Scer\GAL4GMR.PF, results in a 'small eye' phenotype in the adult, caused by the induction of cell death.
Expression of
hpoUAS.cUa under the control of
Scer\GAL4dpp.PU causes undergrowth of the expressing region of the wing disc in the larva. Expression of
hpoUAS.cUa under the control of
Scer\GAL4nub.PU causes an extreme reduction in the size of the adult wing.
hpoScer\UAS.cUa;
Scer\GAL4GMR.PF flies have severely reduced and mispatterned eyes. At midpupal stages, the developing retinas in these animals are disorganised with very reduced numbers of inter-ommatidial cells. Increased levels of cell death are seen in the eye discs of these animals at late third instar.
Overexpression of
hpoScer\UAS.cUa, under the regulation of
Scer\GAL4C5 results in a large increase in apoptosis. Overexpression of
hpoScer\UAS.cUa, under the regulation of
Scer\GAL4C5 during imaginal disc development results in loss of eye, head, and wing tissues through the arrest of cell proliferation and the induction of apoptosis.
