FlyBase Allele Report: Dmel\hpo[UAS.cUa]

General Information

Symbol

Dmel\hpo^UAS.cUa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Udan

FlyBase ID

FBal0191267

Feature type

allele

Associated gene

Dmel\hpo

Associated Insertion(s)

Carried in Construct

P{UAS-hpo.U}

Also Known As

UAS-hpo, UAS.hpo, UAS-Hippo

Key Links

Allele class

Mutagen

in vitro construct - regulatory fusion

Nature of the Allele

Allele class

Mutagen

in vitro construct - regulatory fusion

(Udan et al., 2003)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Carried in construct

P{UAS-hpo.U}

Cytology

Nature of the lesion

Statement

Reference

UASt regulatory sequences drive expression of hpo full-length ORF consisting of residues 1-669. This corresponds to the cDNA clone RE21564.

(Udan et al., 2003)

Allele components

Product class / Tool use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

hpo

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Alzheimer's disease 1

modeled by Hsap\APP^{Aβ42.UAS.cUa}

(Irwin et al., 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate | pharate adult stage, with Scer\GAL4^GMR.PF

(Verghese et al., 2012)

abnormal size | adult stage, with Scer\GAL4^cv-c-C5

(Udan et al., 2003)

abnormal size | adult stage, with Scer\GAL4^nub.PU

(Sansores-Garcia et al., 2011)

abnormal size | adult stage, with Scer\GAL4^unspecified

(Udan et al., 2003)

abnormal size | larval stage, with Scer\GAL4^dpp.PU

(Sansores-Garcia et al., 2011)

decreased occurrence of cell division | larval stage, with Scer\GAL4^cv-c-C5

(Udan et al., 2003)

fertile, with Scer\GAL4^VP16.nos.UTR

(Sun et al., 2008)

increased cell death, with Scer\GAL4^cv-c-C5

(Udan et al., 2003)

increased cell death, with Scer\GAL4^GMR.PF

(Verghese et al., 2012, Nolo et al., 2006, Udan et al., 2003)

increased cell death, with Scer\GAL4^hs.PB

(Verghese et al., 2012)

increased cell death | larval stage, with Scer\GAL4^cv-c-C5

(Udan et al., 2003)

visible, with Scer\GAL4^GMR.PF

(Kanao et al., 2012, Hamaratoglu et al., 2006)

visible, with Scer\GAL4^nub.PU

(Dent et al., 2015)

visible, with Scer\GAL4^nub-AC-62

(Zhao et al., 2007)

Phenotype Manifest In

adult head, with Scer\GAL4^cv-c-C5

(Udan et al., 2003)

eye, with Scer\GAL4^GMR.PF

(Kanao et al., 2012, Verghese et al., 2012, Hamaratoglu et al., 2006)

eye | temperature conditional, with Scer\GAL4^GMR.PF

(Verghese et al., 2012)

eye disc, with Scer\GAL4^GMR.PF

(Verghese et al., 2012)

eye disc | third instar larval stage, with Scer\GAL4^GMR.PF

(Hamaratoglu et al., 2006)

interommatidial bristle, with Scer\GAL4^GMR.PF

(Nolo et al., 2006)

interommatidial cell | pupal stage, with Scer\GAL4^GMR.PF

(Hamaratoglu et al., 2006)

ommatidium, with Scer\GAL4^GMR.PF

(Nolo et al., 2006)

photoreceptor neuron, with Scer\GAL4^GMR.PF

(Verghese et al., 2012)

pigment cell, with Scer\GAL4^GMR.PF

(Verghese et al., 2012)

retina, with Scer\GAL4^GMR.PF

(Verghese et al., 2012)

wing, with Scer\GAL4^cv-c-C5

(Udan et al., 2003)

wing, with Scer\GAL4^nub.PU

(Dent et al., 2015, Sansores-Garcia et al., 2011)

wing, with Scer\GAL4^nub-AC-62

(Zhao et al., 2007, Nolo et al., 2006)

wing disc, with Scer\GAL4^dpp.PU

(Sansores-Garcia et al., 2011)

wing disc, with Scer\GAL4^hs.PB

(Verghese et al., 2012)

Detailed Description

Statement

Reference

Expression of hpo^Scer\UAS.cUa under the control of Scer\GAL4^nub.PU results in a reduction in wing area compared to controls.

(Dent et al., 2015)

Expression of hpo^Scer\UAS.cUa under the control of Scer\GAL4^GMR.PF results in extensive eye degeneration.

(Kanao et al., 2012)

Overexpression of hpo^Scer\UAS.cUa in the GMR domain of the developing eye (under the control of Scer\GAL4^GMR.PF) induces massive cell death without disrupting cell differentiation or patterning. In mid-pupal retina (around 24 hours after pupal formation), both photoreceptor and pigment cells continue to die precociously. In comparison, in wild-type pupal retinae cell death occurs prominently in the pigment cells, and around the retinal boundary. Compared with wild-type adult eyes, hpo^Scer\UAS.cUa overexpressing adult flies are arrested as pharates at room temperature, and at 18[o]C show small rough eyes. hpo^Scer\UAS.cUa overexpression causes apoptosis in all epithelial tissues, for example, in the wing disc short pulses of hpo^Scer\UAS.cUa expression using heat shock (Scer\GAL4^hs.PB) causes apoptosis. In comparison, very few cells undergo apoptosis in wild-type wing discs.

Overexpression of hpo^Scer\UAS.cUa in the eye, under the control of Scer\GAL4^GMR.PF, results in a 'small eye' phenotype in the adult, caused by the induction of cell death.

(Verghese et al., 2012)

Expression of hpo^UAS.cUa under the control of Scer\GAL4^dpp.PU causes undergrowth of the expressing region of the wing disc in the larva. Expression of hpo^UAS.cUa under the control of Scer\GAL4^nub.PU causes an extreme reduction in the size of the adult wing.

(Sansores-Garcia et al., 2011)

Expression of hpo^Scer\UAS.cUa in the germline, under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16}, does not result in germ-cell over-proliferation, or any alteration of cell morphology or loss of fertility.

(Sun et al., 2008)

Expression of hpo^Scer\UAS.cUa under the control of Scer\GAL4^nub-AC-62 results in a small wing phenotype.

(Zhao et al., 2007)

Expression of hpo^Scer\UAS.cUa, under the control of Scer\GAL4^GMR.PF induces apoptosis in the GMR expression domain, posterior to the morphogenetic furrow.

(Bennett and Harvey, 2006)

hpo^Scer\UAS.cUa; Scer\GAL4^GMR.PF flies have severely reduced and mispatterned eyes. At midpupal stages, the developing retinas in these animals are disorganised with very reduced numbers of inter-ommatidial cells. Increased levels of cell death are seen in the eye discs of these animals at late third instar.

(Hamaratoglu et al., 2006)

Overexpression of hpo^Scer\UAS.cUa, under the control of Scer\GAL4^GMR.PF, during eye development induces apoptosis and results in small and rough eyes.

Expression of hpo^Scer\UAS.cUa in the wing, driven by Scer\GAL4^nub-AC-62, causes a reduction in tissue size.

(Nolo et al., 2006)

Overexpression of hpo^Scer\UAS.cUa, under the regulation of Scer\GAL4^C5 results in a large increase in apoptosis. Overexpression of hpo^Scer\UAS.cUa, under the regulation of Scer\GAL4^C5 during imaginal disc development results in loss of eye, head, and wing tissues through the arrest of cell proliferation and the induction of apoptosis.

(Udan et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^nub.PU, hpo^UAS.cUa has visible phenotype, enhanceable by RtGEF^UAS.Tag:HA/Git^UAS.Tag:MYC, Scer\GAL4^nub.PU

(Dent et al., 2015)

Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, hpo^UAS.cUa has increased cell death phenotype, enhanceable by Dronc^pro.UAS, Scer\GAL4^GMR.PF

(Verghese et al., 2012)

Dronc^pro.UAS, Scer\GAL4^GMR.PF, hpo^UAS.cUa has increased cell death phenotype, enhanceable by Dark^{UAS.Tag:polyHis,Tag:MYC}, Scer\GAL4^GMR.PF

(Verghese et al., 2012)

Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, hpo^UAS.cUa has increased cell death phenotype, enhanceable by Dronc^pro.UAS/Dark^{UAS.Tag:polyHis,Tag:MYC}, Scer\GAL4^GMR.PF

(Verghese et al., 2012)

Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, hpo^UAS.cUa has abnormal developmental rate | pharate adult stage phenotype, enhanceable by Dronc^pro.UAS, Scer\GAL4^GMR.PF

(Verghese et al., 2012)

NOT Enhanced by

Statement

Reference

Scer\GAL4^nub.PU, hpo^UAS.cUa has visible phenotype, non-enhanceable by Git^UAS.Tag:MYC, Scer\GAL4^nub.PU

(Dent et al., 2015)

Scer\GAL4^nub.PU, hpo^UAS.cUa has visible phenotype, non-enhanceable by RtGEF^UAS.Tag:HA, Scer\GAL4^nub.PU

(Dent et al., 2015)

Git^UAS.Tag:MYC, RtGEF^UAS.Tag:HA, Scer\GAL4^nub.PU, hpo^UAS.cUa has visible phenotype, non-enhanceable by Pak^KK101874, Scer\GAL4^nub.PU

(Dent et al., 2015)

Git^UAS.Tag:MYC, RtGEF^UAS.Tag:HA, Scer\GAL4^nub.PU, hpo^UAS.cUa has visible phenotype, non-enhanceable by Pak3^GD8481, Scer\GAL4^nub.PU

(Dent et al., 2015)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, hpo^UAS.cUa has increased cell death phenotype, suppressible by yki^UAS.cHa/Scer\GAL4^GMR.PF

(Verghese et al., 2012)

Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, hpo^UAS.cUa has increased cell death phenotype, suppressible by Dronc^pro.C-A.UAS, Scer\GAL4^GMR.PF

(Verghese et al., 2012)

Scer\GAL4^nub-AC-62, hpo^UAS.cUa has visible phenotype, suppressible by yki^Dbo1/yki[+]

(Zhao et al., 2007)

Scer\GAL4^nub-AC-62, hpo^UAS.cUa has visible phenotype, suppressible by yki[+]/yki^Dbo2

(Zhao et al., 2007)

Scer\GAL4^nub-AC-62, hpo^UAS.cUa has visible phenotype, suppressible by yki[+]/yki^Dbo3

(Zhao et al., 2007)

Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, hpo^UAS.cUa has increased cell death phenotype, suppressible by mir-ban^EP3622/Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF

(Nolo et al., 2006)

Scer\GAL4^cv-c-C5/Scer\GAL4^C5, hpo^UAS.cUa has increased cell death phenotype, suppressible | partially by wts^x1/wts[+]

(Udan et al., 2003)

Scer\GAL4^cv-c-C5/Scer\GAL4^C5, hpo^UAS.cUa has increased cell death phenotype, suppressible | partially by BacA\p35^UAS.cHa, Scer\GAL4^cv-c-C5/Scer\GAL4^C5

(Udan et al., 2003)

Scer\GAL4^cv-c-C5/Scer\GAL4^C5, hpo^UAS.cUa has increased cell death phenotype, suppressible | partially by Scer\GAL4^cv-c-C5/Scer\GAL4^C5/Diap1^UAS.cHa

(Udan et al., 2003)

Scer\GAL4^cv-c-C5, hpo^UAS.cUa has increased cell death | larval stage phenotype, suppressible by wts^x1

(Udan et al., 2003)

Scer\GAL4^cv-c-C5, hpo^UAS.cUa has increased cell death | larval stage phenotype, suppressible by hid^unspecified

(Udan et al., 2003)

Scer\GAL4^cv-c-C5, hpo^UAS.cUa has abnormal size | adult stage phenotype, suppressible by hid^unspecified

(Udan et al., 2003)

Scer\GAL4^unspecified, hpo^UAS.cUa has abnormal size | adult stage phenotype, suppressible by hid^unspecified

(Udan et al., 2003)

Scer\GAL4^cv-c-C5, hpo^UAS.cUa has abnormal size | adult stage phenotype, suppressible | partially by hid^A206/hid[+]

(Udan et al., 2003)

Scer\GAL4^unspecified, hpo^UAS.cUa has abnormal size | adult stage phenotype, suppressible | partially by hid^A206/hid[+]

(Udan et al., 2003)

Scer\GAL4^cv-c-C5, hpo^UAS.cUa has abnormal size | adult stage phenotype, suppressible | partially by Tsc1[+]/Tsc1^unspecified

(Udan et al., 2003)

Scer\GAL4^unspecified, hpo^UAS.cUa has abnormal size | adult stage phenotype, suppressible | partially by Tsc1[+]/Tsc1^unspecified

(Udan et al., 2003)

Scer\GAL4^cv-c-C5, hpo^UAS.cUa has abnormal size | adult stage phenotype, suppressible by BacA\p35^UAS.cHa

(Udan et al., 2003)

Scer\GAL4^cv-c-C5/Scer\GAL4^C5, hpo^UAS.cUa has increased cell death phenotype, suppressible | partially by hid^A206/W[+]

(Udan et al., 2003)

Scer\GAL4^unspecified, hpo^UAS.cUa has abnormal size | adult stage phenotype, suppressible by BacA\p35^UAS.cHa

(Udan et al., 2003)

NOT suppressed by

Statement

Reference

Git^UAS.Tag:MYC, RtGEF^UAS.Tag:HA, Scer\GAL4^nub.PU, hpo^UAS.cUa has visible phenotype, non-suppressible by Pak^KK101874, Scer\GAL4^nub.PU

(Dent et al., 2015)

Git^UAS.Tag:MYC, RtGEF^UAS.Tag:HA, Scer\GAL4^nub.PU, hpo^UAS.cUa has visible phenotype, non-suppressible by Pak3^GD8481, Scer\GAL4^nub.PU

(Dent et al., 2015)

hpo^UAS.cUa has visible phenotype, non-suppressible by ex^BQ/ex^BQ

(Hamaratoglu et al., 2006)

Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, hpo^UAS.cUa has increased cell death phenotype, non-suppressible by ex[+]/ex^BQ/Mer[+]/Mer⁴

(Hamaratoglu et al., 2006)

Scer\GAL4^cv-c-C5/Scer\GAL4^C5, hpo^UAS.cUa has increased cell death phenotype, non-suppressible by Tsc1^IQ69/Tsc1[+]

(Udan et al., 2003)

Suppressor of

Statement

Reference

hpo^UAS.cUa/Scer\GAL4^GMR.PFa is a suppressor | partially of visible | adult stage phenotype of Scer\GAL4^GMR.PFa, yki^{S168A.UAS.EGFP,Tag:HA}

(Yeom et al., 2020)

hpo^UAS.cUa/Scer\GAL4^GMR.PFa is a suppressor | partially of neoplasia | adult stage phenotype of Scer\GAL4^GMR.PFa, yki^{S168A.UAS.EGFP,Tag:HA}

(Yeom et al., 2020)

Scer\GAL4^dpp.PU/hpo^UAS.cUa is a suppressor | partially of neoplasia | larval stage phenotype of Scer\GAL4^dpp.PU, dia^{CA.UAS.Tag:HA}

(Sansores-Garcia et al., 2011)

Other

Statement

Reference

Git^UAS.Tag:MYC, RtGEF^UAS.Tag:HA, Scer\GAL4^nub.PU, hpo^UAS.cUa has visible phenotype

(Dent et al., 2015)

BacA\p35^UAS.cHa, Scer\GAL4^C855a, hpo^UAS.cUa has abnormal neuroanatomy | larval stage phenotype

(Richter et al., 2011)

Scer\GAL4^Tub.PU, hpo^UAS.cUa, Diap1^UAS.cHa has abnormal cell shape | somatic clone phenotype

(Thompson and Cohen, 2006)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^nub.PU, hpo^UAS.cUa has wing phenotype, enhanceable by RtGEF^UAS.Tag:HA/Git^UAS.Tag:MYC, Scer\GAL4^nub.PU

(Dent et al., 2015)

Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, hpo^UAS.cUa has eye phenotype, enhanceable by Dronc^pro.UAS, Scer\GAL4^GMR.PF

(Verghese et al., 2012)

Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, hpo^UAS.cUa has eye-antennal disc phenotype, enhanceable by Dronc^pro.UAS/Dark^{UAS.Tag:polyHis,Tag:MYC}, Scer\GAL4^GMR.PF

(Verghese et al., 2012)

Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, hpo^UAS.cUa has pigment cell phenotype, enhanceable by Dronc^pro.UAS/Dark^{UAS.Tag:polyHis,Tag:MYC}, Scer\GAL4^GMR.PF

(Verghese et al., 2012)

Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, hpo^UAS.cUa has photoreceptor neuron phenotype, enhanceable by Dronc^pro.UAS/Dark^{UAS.Tag:polyHis,Tag:MYC}, Scer\GAL4^GMR.PF

(Verghese et al., 2012)

Dronc^pro.UAS, Scer\GAL4^GMR.PF, hpo^UAS.cUa has eye-antennal disc phenotype, enhanceable by Dark^{UAS.Tag:polyHis,Tag:MYC}, Scer\GAL4^GMR.PF

(Verghese et al., 2012)

Dronc^pro.UAS, Scer\GAL4^GMR.PF, hpo^UAS.cUa has pigment cell phenotype, enhanceable by Dark^{UAS.Tag:polyHis,Tag:MYC}, Scer\GAL4^GMR.PF

(Verghese et al., 2012)

Dronc^pro.UAS, Scer\GAL4^GMR.PF, hpo^UAS.cUa has photoreceptor neuron phenotype, enhanceable by Dark^{UAS.Tag:polyHis,Tag:MYC}, Scer\GAL4^GMR.PF

(Verghese et al., 2012)

Dronc^pro.UAS, Scer\GAL4^GMR.PF, hpo^UAS.cUa has eye phenotype, enhanceable by Dark^{UAS.Tag:polyHis,Tag:MYC}, Scer\GAL4^GMR.PF

(Verghese et al., 2012)

NOT Enhanced by

Statement

Reference

Scer\GAL4^nub.PU, hpo^UAS.cUa has wing phenotype, non-enhanceable by Git^UAS.Tag:MYC, Scer\GAL4^nub.PU

(Dent et al., 2015)

Scer\GAL4^nub.PU, hpo^UAS.cUa has wing phenotype, non-enhanceable by RtGEF^UAS.Tag:HA, Scer\GAL4^nub.PU

(Dent et al., 2015)

Git^UAS.Tag:MYC, RtGEF^UAS.Tag:HA, Scer\GAL4^nub.PU, hpo^UAS.cUa has wing phenotype, non-enhanceable by Pak^KK101874, Scer\GAL4^nub.PU

(Dent et al., 2015)

Git^UAS.Tag:MYC, RtGEF^UAS.Tag:HA, Scer\GAL4^nub.PU, hpo^UAS.cUa has wing phenotype, non-enhanceable by Pak3^GD8481, Scer\GAL4^nub.PU

(Dent et al., 2015)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, hpo^UAS.cUa has retina phenotype, suppressible by yki^UAS.cHa/Scer\GAL4^GMR.PF

(Verghese et al., 2012)

Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, hpo^UAS.cUa has eye disc phenotype, suppressible by yki^UAS.cHa/Scer\GAL4^GMR.PF

(Verghese et al., 2012)

Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, hpo^UAS.cUa has photoreceptor neuron phenotype, suppressible by yki^UAS.cHa/Scer\GAL4^GMR.PF

(Verghese et al., 2012)

Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, hpo^UAS.cUa has pigment cell phenotype, suppressible by yki^UAS.cHa/Scer\GAL4^GMR.PF

(Verghese et al., 2012)

Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, hpo^UAS.cUa has eye phenotype, suppressible by yki^UAS.cHa/Scer\GAL4^GMR.PF

(Verghese et al., 2012)

Scer\GAL4^nub-AC-62, hpo^UAS.cUa has wing phenotype, suppressible by yki^Dbo1/yki[+]

(Zhao et al., 2007)

Scer\GAL4^nub-AC-62, hpo^UAS.cUa has wing phenotype, suppressible by yki[+]/yki^Dbo2

(Zhao et al., 2007)

Scer\GAL4^nub-AC-62, hpo^UAS.cUa has wing phenotype, suppressible by yki[+]/yki^Dbo3

(Zhao et al., 2007)

Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, hpo^UAS.cUa has interommatidial cell | pupal stage phenotype, suppressible by wts^x1/wts^x1

(Hamaratoglu et al., 2006)

Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, hpo^UAS.cUa has ommatidium phenotype, suppressible by mir-ban^EP3622/Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF

(Nolo et al., 2006)

Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, hpo^UAS.cUa has interommatidial bristle phenotype, suppressible by mir-ban^EP3622/Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF

(Nolo et al., 2006)

hpo^UAS.cUa has wing phenotype, suppressible by Scer\GAL4^nub-AC-62/mir-ban^EP3622

(Nolo et al., 2006)

Scer\GAL4^cv-c-C5/Scer\GAL4^C5, hpo^UAS.cUa has wing phenotype, suppressible | partially by Scer\GAL4^cv-c-C5/Scer\GAL4^C5/Diap1^UAS.cHa

(Udan et al., 2003)

Scer\GAL4^cv-c-C5/Scer\GAL4^C5, hpo^UAS.cUa has adult head phenotype, suppressible | partially by Scer\GAL4^cv-c-C5/Scer\GAL4^C5/Diap1^UAS.cHa

(Udan et al., 2003)

Scer\GAL4^cv-c-C5/Scer\GAL4^C5, hpo^UAS.cUa has wing phenotype, suppressible | partially by BacA\p35^UAS.cHa, Scer\GAL4^cv-c-C5/Scer\GAL4^C5

(Udan et al., 2003)

Scer\GAL4^cv-c-C5/Scer\GAL4^C5, hpo^UAS.cUa has adult head phenotype, suppressible | partially by BacA\p35^UAS.cHa, Scer\GAL4^cv-c-C5/Scer\GAL4^C5

(Udan et al., 2003)

Scer\GAL4^cv-c-C5/Scer\GAL4^C5, hpo^UAS.cUa has wing phenotype, suppressible | partially by hid^A206/W[+]

(Udan et al., 2003)

Scer\GAL4^cv-c-C5/Scer\GAL4^C5, hpo^UAS.cUa has adult head phenotype, suppressible | partially by hid^A206/W[+]

(Udan et al., 2003)

Scer\GAL4^cv-c-C5/Scer\GAL4^C5, hpo^UAS.cUa has wing phenotype, suppressible | partially by wts^x1/wts[+]

(Udan et al., 2003)

NOT suppressed by

Statement

Reference

Git^UAS.Tag:MYC, RtGEF^UAS.Tag:HA, Scer\GAL4^nub.PU, hpo^UAS.cUa has wing phenotype, non-suppressible by Pak^KK101874, Scer\GAL4^nub.PU

(Dent et al., 2015)

Git^UAS.Tag:MYC, RtGEF^UAS.Tag:HA, Scer\GAL4^nub.PU, hpo^UAS.cUa has wing phenotype, non-suppressible by Pak3^GD8481, Scer\GAL4^nub.PU

(Dent et al., 2015)

Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, hpo^UAS.cUa has eye phenotype, non-suppressible by ex^BQ/ex^BQ

(Hamaratoglu et al., 2006)

Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, hpo^UAS.cUa has interommatidial cell | pupal stage phenotype, non-suppressible by ex^BQ/ex^BQ

(Hamaratoglu et al., 2006)

Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, hpo^UAS.cUa has interommatidial cell | pupal stage phenotype, non-suppressible | somatic clone by ex^BQ/ex^BQ/Mer⁴/Mer⁴

(Hamaratoglu et al., 2006)

Scer\GAL4^cv-c-C5/Scer\GAL4^C5, hpo^UAS.cUa has wing phenotype, non-suppressible by Tsc1^IQ69/Tsc1[+]

(Udan et al., 2003)

Scer\GAL4^cv-c-C5/Scer\GAL4^C5, hpo^UAS.cUa has adult head phenotype, non-suppressible by Tsc1^IQ69/Tsc1[+]

(Udan et al., 2003)

Suppressor of

Statement

Reference

hpo^UAS.cUa/Scer\GAL4^GMR.PFa is a suppressor | partially of eye phenotype of Scer\GAL4^GMR.PFa, yki^{S168A.UAS.EGFP,Tag:HA}

(Yeom et al., 2020)

Scer\GAL4^dpp.PU/hpo^UAS.cUa is a suppressor | partially of wing disc phenotype of Scer\GAL4^dpp.PU, dia^{CA.UAS.Tag:HA}

(Sansores-Garcia et al., 2011)

Other

Statement

Reference

Git^UAS.Tag:MYC, RtGEF^UAS.Tag:HA, Scer\GAL4^nub.PU, hpo^UAS.cUa has wing phenotype

(Dent et al., 2015)

Git^UAS.Tag:MYC, RtGEF^UAS.Tag:HA, Scer\GAL4^nub.PU, hpo^UAS.cUa has eye phenotype

(Dent et al., 2015)

BacA\p35^UAS.cHa, Scer\GAL4^C855a, hpo^UAS.cUa has embryonic/larval optic lobe | larval stage phenotype

(Richter et al., 2011)

Scer\GAL4^ptc-559.1, hpo^UAS.cUa, Diap1^UAS.cHa has wing disc phenotype

(Thompson and Cohen, 2006)

Scer\GAL4^Tub.PU, hpo^UAS.cUa, Diap1^UAS.cHa has wing disc | somatic clone phenotype

(Thompson and Cohen, 2006)

Additional Comments

Genetic Interactions

Statement

Reference

The reduction in wing area seen in flies expressing hpo^Scer\UAS.cUa under the control of Scer\GAL4^nub.PU is not enhanced by co-expression of either RtGEF^{Scer\UAS.T:Ivir\HA1} or Git^{Scer\UAS.T:Hsap\MYC} alone, but it is significantly enhanced by simultaneous co-expression of both RtGEF^{Scer\UAS.T:Ivir\HA1} and Git^{Scer\UAS.T:Hsap\MYC} together.

The strong reduction in wing area seen in flies co-expressing all of hpo^Scer\UAS.cUa, RtGEF^{Scer\UAS.T:Ivir\HA1} and Git^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^nub.PU is unaffected if they are also carrying either Pak^KK101874 or Pak3^GD8481.

(Dent et al., 2015)

Co-expression of hpo^Scer\UAS.cUa and yki^Scer\UAS.cHa in eye-antennal discs, under the control of Scer\GAL4^GMR.PF, completely rescues the small eye phenotype of hpo activation.

Co-expression of Nc^pro.Scer\UAS with hpo^Scer\UAS.cUa in the developing eye under the control of Scer\GAL4^GMR.PF results in increased cell death, compared to either single mutant. These larvae develop into pharate adult stages with small eyes.

Co-expression of Nc^{pro.C-A.Scer\UAS} with hpo^Scer\UAS.cUa in the developing eye (both under the control of Scer\GAL4^GMR.PF completely suppresses the cell death seen upon expression of hpo^Scer\UAS.cUa alone.

Co-expression of Ark^{Scer\UAS.T:Zzzz\His6,T:Hsap\MYC}, Nc^pro.Scer\UAS and hpo^Scer\UAS.cUa in the developing eye, under the control of Scer\GAL4^GMR.PF induces massive apoptosis in the eye-antennal disc and adult. This phenotype appears stronger than controls as both photoreceptor and pigment cells are eventually eliminated in animals co-expressing hpo^Scer\UAS.cUa.

(Verghese et al., 2012)

The small wing phenotype caused by expression of hpo^Scer\UAS.cUa under the control of Scer\GAL4^nub-AC-62 is strongly suppressed by one copy of yki^Dbo1, yki^Dbo2 or yki^Dbo3.

(Zhao et al., 2007)

In eye-discs mosaic for ft⁸, the expression hpo^Scer\UAS.cUa under the control of Scer\GAL4^GMR.PF induces apoptosis as in wild-type tissue.

(Bennett and Harvey, 2006)

The adult eye phenotype and the loss of interommmatidial cells seen in hpo^Scer\UAS.cUa; Scer\GAL4^GMR.PF mid-pupal retinas is not suppressed by ex^BQ/ex^BQ or in ex^BQ Mer⁴ double homozygous clones, but is suppressed by wts^x1/wts^x1. The resulting animals have an excess of inter-ommatidial cells, as in wts^x1/wts^x1 animals.

The increase in cell death seen in late third instar eye discs from hpo^Scer\UAS.cUa; Scer\GAL4^GMR.PF animals is not suppressed by ex^BQ/+; Mer⁴/+.

(Hamaratoglu et al., 2006)

Coexpression of ban^EP3622 with hpo^Scer\UAS.cUa, both driven by Scer\GAL4^GMR.PF, significantly suppresses the eye defects and eliminates the apoptosis caused by hpo overexpression.

Coexpression of ban^EP3622 with hpo^Scer\UAS.cUa in the wing, driven by Scer\GAL4^nub-AC-62, suppresses the Scer\GAL4^nub-AC-62>hpo^Scer\UAS.cUa small wing phenotype.

(Nolo et al., 2006)

Coexpression of th^Scer\UAS.cHa and hpo^Scer\UAS.cUa, under the control of Scer\GAL4^ptc-559.1, in wing discs leads to a folding of the epithelium in the ptc expression region.

Clones in the wing disc that express hpo^Scer\UAS.cUa, under the control of Scer\GAL4^tub, (rescued for apoptosis by expression of th^Scer\UAS.cHa) cause nuclei to move basally, inducing cells to change shape and causing a basally-directed folding of the epithelium.

(Thompson and Cohen, 2006)

Apoptosis induced by overexpression of hpo^Scer\UAS.cUa, under the regulation of Scer\GAL4^unspecified is suppressed by coexpression of th^unspecified. The small head and wing phenotypes observed in hpo^Scer\UAS.cUa (under the regulation of Scer\GAL4^unspecified or Scer\GAL4^C5) can be partially suppressed by coexpression of th^unspecified and BacA\p35^Scer\UAS.cHa. The small wing and eye phenotypes caused by hpo^Scer\UAS.cUa overexpression, (through Scer\GAL4^unspecified or Scer\GAL4^C5) can be partially suppressed by heterozygosity for W^hid-A206, indicating that hpo requires W for its action. However, heterozygosity for Tsc1^unspecified does not affect the hpo^Scer\UAS.cUa overexpression phenotype. The Scer\GAL4^C5-driven hpo^Scer\UAS.cUa cell death phenotype observed in the wing can be partially suppressed through wts^x1 heterozygosity.

(Udan et al., 2003)

The small head and wing phenotypes, caused by an increase in apoptosis, seen in flies that express hpo^Scer\UAS.cUa under the control of Scer\GAL4^C5 is partially rescued by coexpression of th^Scer\UAS.cHa.

The small wing and eye phenotypes due to an increase in apoptosis caused by hpo^Scer\UAS.cUa overexpression, driven by Scer\GAL4^C5) can be partially suppressed by heterozygosity for W^hid-A206, indicating that hpo requires W for its action. However, heterozygosity for Tsc1^IQ69 does not affect the hpo^Scer\UAS.cUa overexpression phenotype.

The Scer\GAL4^C5-driven hpo^Scer\UAS.cUa cell death phenotype observed in the wing can be partially suppressed through wts^x1 heterozygosity.

(Udan et al., 2003)

Xenogenetic Interactions

Statement

Reference

Co-expression of hpo^Scer\UAS.cUa and BacA\p35^Scer\UAS.cHa under the control of Scer\GAL4^C855a results in underproliferation of the optic lobe neuroepithelia in larvae.

(Richter et al., 2011)

(Udan et al., 2003)

Complementation and Rescue Data

Rescued by

hpo^UAS.cUa is rescued by Scer\GAL4^cv-c-C5/hpo^K71R.UAS.cUa/Scer\GAL4^C5

(Udan et al., 2003)

Comments

Apoptosis induced by overexpression of hpo^Scer\UAS.cUa, under the regulation of Scer\GAL4^C5 is rescued by coexpression of hpo^{K71R.Scer\UAS.cUa}.

(Udan et al., 2003)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

hpo^Scer\UAS.cUa

hpo^UAS.cUa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Udan

Secondary FlyBase IDs

References (24)

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