peloUASp.cXa/Scer\GAL4VP16.nos.UTR partially rescues pelo1/peloPB60
peloUASp.cXa/Scer\GAL4da.PU partially rescues peloΔ
peloUASp.cXa/Scer\GAL4[-] partially rescues pelo1
peloUASp.cXa/Scer\GAL4C587 partially rescues pelo1
Expressing peloUASp.cXa under the control of Scer\GAL4VP16.nos.UTR fully rescues the male sterility and spermatogenesis defects (male germline stem cell number, individualization complex formation and spermatid nuclei elongation defects) observed in peloPB60/pelo1 transheterozygotes.
The near complete sterility (minimal number of laid eggs, very low hatching rate) of pelo1/peloPB60 transheterozygous females can be partially rescued by expression of peloScer\UAS.P\T.cXa under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 in the mutant background. Expression under the Scer\GAL4nos.UTR.T:Hsim\VP16 driver rescues the spermatogenesis defects (no individualization complexes and waste bags) as well as the male sterility, which can also bes rescued by Scer\GAL4αTub84B.PL-driven expression.
Expression of peloScer\UAS.P\T.cXa under the control of Scer\GAL4da.PU suppresses the resistance of peloΔ homozygous mutants to the Drosophila C virus, rendering them sensitive to the infection.
The GSC loss phenotype in pelo1 females is partially rescued by leaky expression of the peloScer\UAS.P\T.cXa transgene without a driver; expression driven by Scer\GAL4C587 in the somatic cells of the germarium confers very limited extra rescue compared to that conferred by the transgene alone.