FB2021_01, released Feb 18, 2021

Allele: Dmel\peloUASp.cXa

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General Information
Symbol
Dmel\peloUASp.cXa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Xi
FlyBase ID
FBal0191293
Feature type
allele
Associated gene
Dmel\pelo
Associated Insertion(s)
Carried in Construct
P{UASp-pelo.X}
Also Known As
UASp-pelo, UAS-pelo
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
in vitro construct - regulatory fusion
(Xi et al., 2005)
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
       
      Progenitor genotype
      Carried in construct
      P{UASp-pelo.X}
      Cytology
      Nature of the lesion
      Statement
      Reference

      UASp regulatory sequences drive expression of pelo. The pelo sequence corresponds to the AT07625 clone.

      (Xi et al., 2005)
      Allele components
      Product class / Tool use(s)
      Regulatory region(s)
      UASp
      Encoded product / tool
      pelo
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      peloUASp.cXa/Scer\GAL4VP16.nos.UTR rescues pelo1/peloPB60

      (Li et al., 2019)

      peloUASp.cXa/Scer\GAL4αTub84B.PL rescues pelo1/peloPB60

      (Yang et al., 2015)

      peloUASp.cXa/Scer\GAL4VP16.nos.UTR rescues pelo1

      (Xi et al., 2005)
      Partially rescues

      peloUASp.cXa/Scer\GAL4VP16.nos.UTR partially rescues pelo1/peloPB60

      (Yang et al., 2015)

      peloUASp.cXa/Scer\GAL4da.PU partially rescues peloΔ

      (Wu et al., 2014)

      peloUASp.cXa/Scer\GAL4[-] partially rescues pelo1

      (Xi et al., 2005)

      peloUASp.cXa/Scer\GAL4C587 partially rescues pelo1

      (Xi et al., 2005)
      Comments

      Expressing peloUASp.cXa under the control of Scer\GAL4VP16.nos.UTR fully rescues the male sterility and spermatogenesis defects (male germline stem cell number, individualization complex formation and spermatid nuclei elongation defects) observed in peloPB60/pelo1 transheterozygotes.

      (Li et al., 2019)

      The near complete sterility (minimal number of laid eggs, very low hatching rate) of pelo1/peloPB60 transheterozygous females can be partially rescued by expression of peloScer\UAS.P\T.cXa under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 in the mutant background. Expression under the Scer\GAL4nos.UTR.T:Hsim\VP16 driver rescues the spermatogenesis defects (no individualization complexes and waste bags) as well as the male sterility, which can also bes rescued by Scer\GAL4αTub84B.PL-driven expression.

      (Yang et al., 2015)

      Expression of peloScer\UAS.P\T.cXa under the control of Scer\GAL4da.PU suppresses the resistance of peloΔ homozygous mutants to the Drosophila C virus, rendering them sensitive to the infection.

      (Wu et al., 2014)

      The GSC loss phenotype in pelo1 females is partially rescued by leaky expression of the peloScer\UAS.P\T.cXa transgene without a driver; expression driven by Scer\GAL4C587 in the somatic cells of the germarium confers very limited extra rescue compared to that conferred by the transgene alone.

      (Xi et al., 2005)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      peloScer\UAS.P\T.cXa
      peloUASp.cXa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Xi
      (Xi et al., 2005)
      Secondary FlyBase IDs
        References (6)