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General Information
Symbol
Dmel\cidEGFP
Species
D. melanogaster
Name
FlyBase ID
FBal0191314
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
EGFP-Cid, gcid-EGFP-cid
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

A genomic fragment of cid is expressed from its own promoter and has EGFP fused within the fragment 11 amino acids before the start of the histone fold.

P{gcid-EGFP-cid} is composed of three distinct fragments. First, a 772 bp cid PCR fragment encompassing 412 bp upstream of the cid translational initiation codon and 354 bp of the coding region up to codon 118. Second, a PCR fragment encompassing the cid region coding for amino acids 119 through 225 as well as 513 bp of the flanking 3' genomic region. A third fragment encompassing the EGFP coding region, and flanked by three glycine residues at both sides to serve as flexible linkers, is inserted between the cid codons specifying amino acids 118 and 119. Thus, EGFP is inserted within the N-terminal tail of the cid protein.

Allele components
Product class / Tool use(s)
Regulatory region(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

When provided by the mother, a single copy of cidT:Avic\GFP-EGFP (insertion line III.2) rescues the lethality of cidT12-1/cidT22-4 transheterozygotes with high efficiency (88% of expected).

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
cidEGFP
cidT:Avic\GFP-EGFP
Name Synonyms
Secondary FlyBase IDs
    References (17)