Allele Dmel\cv-c^Scer\UAS.cDa

General Information
Symbol	Dmel\cv-c^Scer\UAS.cDa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Denholm	FlyBase ID	FBal0191512
Feature type	allele	Associated gene	Dmel\cv-c

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Denholm et al., 2005)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS sequences drive expression of full-length cv-c. (Denholm et al., 2005)
Carried in construct	P{UAS-cv-c.D} (Denholm et al., 2005, Simoes et al., 2006, Brodu and Casanova, 2006, Pilgram et al., 2011)
Cytology
Phenotypic Data
Phenotypic Class
	neurophysiology defective, with Scer\GAL4^Rapgap1-OK6, cv-c¹ (Pilgram et al., 2011)
Phenotype Manifest In
	embryonic Malpighian tubule, with Scer\GAL4^ct.CtB (Denholm et al., 2005) embryonic Malpighian tubule tip cell, with Scer\GAL4^ct.CtB (Denholm et al., 2005) neuromuscular junction, with Scer\GAL4^Rapgap1-OK6, cv-c¹ (Pilgram et al., 2011) posterior spiracle primordium, with Scer\GAL4^ems.HRE (Simoes et al., 2006)
Detailed Description
	Statement Reference Expression of cv-c[Scer\UAS.cDa] in motoneurons under the control of Scer\GAL4[OK6] fails to rescue the abnormal quantal content (QC) phenotype of cv-c[1]/+ larvae. Expression of cv-c[Scer\UAS.cDa] in muscle under the control of Scer\GAL4[G14] rescues the abnormal quantal content (QC) phenotype of cv-c[1]/+ larvae. (Pilgram et al., 2011) Expression of cv-c^Scer\UAS.cDa under the control of Scer\GAL4^ems.HRE results in a failure of invagination of the most distal cells of the posterior spiracular chamber. (Simoes et al., 2006) Malpighian tubules that express cv-c^Scer\UAS.cDa under the control of Scer\GAL4^ct.CtB are significantly shorter in length and fatter around their circumference than wild-type tubules. The length of the tip cell is increased; the tip cell stalk is ~7μ in wild-type tubules, but can be up to 50μ in Scer\GAL4^ct.CtB>cv-c^Scer\UAS.cDa tubules. (Denholm et al., 2005)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Suppressor of
Statement Reference Scer\GAL4^G14/cv-c^Scer\UAS.cDa is a non-suppressor of neurophysiology defective \| dominant phenotype of Dys^E6 (Pilgram et al., 2011)
Phenotype Manifest In
Suppressor of
Statement Reference cv-c^Scer\UAS.cDa/Scer\GAL4^69B is a suppressor \| partially of presumptive embryonic/larval tracheal system phenotype of Egfr^f2 (Brodu and Casanova, 2006) cv-c^Scer\UAS.cDa/Scer\GAL4^69B is a suppressor \| partially of tracheal primordium phenotype of Egfr^f2 (Brodu and Casanova, 2006)
NOT Suppressor of
Statement Reference Scer\GAL4^G14/cv-c^Scer\UAS.cDa is a non-suppressor of neuromuscular junction phenotype of Dys^E6 (Pilgram et al., 2011)
Additional Comments
Genetic Interactions
Statement Reference The increased quantal content (QC) measured at the neuromuscular junction of Dys[E6]/+ mutants is not reduced as a result of expression of cv-c[Scer\UAS.cDa] under the control of Scer\GAL4[G14]. (Pilgram et al., 2011) Expression of cv-c^Scer\UAS.cDa, under the control of Scer\GAL4^69B partially suppresses the Egfr^f2 mutant invagination phenotype. (Brodu and Casanova, 2006)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	Scer\GAL4^G14/cv-c^Scer\UAS.cDa rescues cv-c¹ (Pilgram et al., 2011)
Partially rescues	Scer\GAL4^ct.CtB/cv-c^Scer\UAS.cDa partially rescues cv-c^M62 (Denholm et al., 2005)
Fails to rescue	Scer\GAL4^Rapgap1-OK6/cv-c^Scer\UAS.cDa fails to rescue cv-c¹ (Pilgram et al., 2011)
Comments	Expression of cv-c[Scer\UAS.cDa] in motoneurons under the control of Scer\GAL4[OK6] fails to rescue the abnormal quantal content (QC) phenotype of cv-c[1]/+ larvae. Expression of cv-c[Scer\UAS.cDa] in muscle under the control of Scer\GAL4[G14] rescues the abnormal quantal content (QC) phenotype of cv-c[1]/+ larvae. (Pilgram et al., 2011) Expression of cv-c^Scer\UAS.cDa, under the control of Scer\GAL4^ct.CtB, partially rescues the Malpighian tubule phenotype of cv-c^M62 embryos. (Denholm et al., 2005)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	cv-c^Scer\UAS.cDa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Denholm (Denholm et al., 2005)
Secondary FlyBase IDs

References ( 4 )
Research paper	Pilgram et al., 2011, J. Neurosci. 31(2): 492--500 The RhoGAP crossveinless-c Interacts with Dystrophin and Is Required for Synaptic Homeostasis at the Drosophila Neuromuscular Junction. [FBrf0212767] Brodu and Casanova, 2006, Genes Dev. 20(13): 1817--1828 The RhoGAP crossveinless-c links trachealess and EGFR signaling to cell shape remodeling in Drosophila tracheal invagination. [FBrf0195282] Simoes et al., 2006, Development 133(21): 4257--4267 Compartmentalisation of Rho regulators directs cell invagination during tissue morphogenesis. [FBrf0192475] Denholm et al., 2005, Development 132(10): 2389--2400 crossveinless-c is a RhoGAP required for actin reorganisation during morphogenesis. [FBrf0187455]

Allele Dmel\cv-cScer\UAS.cDa

Allele Dmel\cv-c^Scer\UAS.cDa